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ZrO₂@C-based colorimetric/photothermal dual-mode immunosensor coupled with a novel monoclonal antibody for quantification of Aspergillus ochraceus biomass.

Chen Wang·Jingjing Zhang·Yong-Huan Yun·Qi Zhang·Peiwu Li·Xiaoqian Tang
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Abstract

Aspergillus ochraceus contaminates agricultural products and produces nephrotoxic, carcinogenic ochratoxin A (OTA), posing severe food safety hazards. A dual-signal lateral flow immunochromatographic assay (dLFIA) based on ZrO₂@C nanoprobes was established for quantitative detection of A. ochraceus biomass. A novel monoclonal antibody (mAb 4B4) was prepared as the capture antibody to immobilize A. ochraceus mycelial lysate antigen on the test line, and a rabbit polyclonal antibody (pAb G2801) as the detection antibody to modify ZrO₂@C composites (synthesized via UiO-66 pyrolysis) into 200 nm colorimetric/photothermal nanoprobes. This dLFIA achieved limits of detection of 0.164 μg/mL (colorimetric) and 0.517 μg/mL (photothermal). This efficient and reliable method allows quantitative analysis of A. ochraceus biomass, which is suitable for routine monitoring of fungal contamination in agro-food matrices.

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Antibodies, MonoclonalAspergillus ochraceusColorimetryFood ContaminationZirconiumChromatography, AffinityBiomassImmunoassayAnimalsLateral flow immunochromatographyMetal-organic frameworkJournal ArticleEvaluation Study

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Chen Wang, Jingjing Zhang, Yong-Huan Yun, Qi Zhang, Peiwu Li, Xiaoqian Tang. 2026-07-11. ZrO₂@C-based colorimetric/photothermal dual-mode immunosensor coupled with a novel monoclonal antibody for quantification of Aspergillus ochraceus biomass.. https://doi.org/10.1016/j.foodchem.2026.150381

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