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PubMed · 12605863

A quantitative diffraction-based sandwich immunoassay.

Jane B Goh·Pui L Tam·Richard W Loo·M Cynthia Goh
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Abstract

It is shown that diffraction-based sensing can be enhanced for diagnostic purposes through the use of a secondary label. The limit of detection for anti-rabbit IgG was reduced more than 40-fold by using a gold-conjugated secondary antibody. The response to secondary antibody binding was linear for concentrations from 25 to 500 ng/ml of anti-rabbit IgG, suggesting that quantitative determinations can be readily done. Moreover, the binding of the secondary antibody was observed as soon as 1 min after its introduction to the surface-bound primary complex.

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AnimalsAntibodiesAntibody SpecificityGoldImmunoassayImmunoglobulin GLinear ModelsMiceOptics and PhotonicsProtein BindingRabbitsReproducibility of ResultsSensitivity and SpecificityJournal Article

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Jane B Goh, Pui L Tam, Richard W Loo, M Cynthia Goh. 2003-02-15. A quantitative diffraction-based sandwich immunoassay.. https://doi.org/10.1016/s0003-2697(02)00634-6

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