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Genomic Profiling of Chromatin State Using CUT&Tag.

Fanju W Meng·Duy K Nguyen·Patrick J Murphy
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Abstract

Alterations in chromatin state, mediated through histone modifications and the incorporation of histone variants, are fundamental to establishing transcriptional networks and cell identity. Recent advances in low-input epigenome profiling methods, such as CUT&Tag and CUT&RUN, have enabled the study of chromatin states from very limited starting materials. In this chapter, we describe procedures for generating CUT&Tag libraries to profile histone modifications and histone variants in early-developing zebrafish embryos.

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AnimalsZebrafishChromatinHistonesHistone CodeGenomicsEpigenesis, GeneticEpigenomicsCUT&TagEmbryogenesisEpigeneticsEpigenomeHistone modificationsHistone variantsJournal Article

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Fanju W Meng, Duy K Nguyen, Patrick J Murphy. 2027. Genomic Profiling of Chromatin State Using CUT&Tag.. https://doi.org/10.1007/978-1-0716-5539-9_16

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