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PubMed · 6376831

Decrease in rabbit bladder mucosal glycoprotein after oophorectomy.

K Weisman·H Callahan·H S Cooper·R W Fritz·S G Mulholland
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Abstract

Hormonal manipulation has been shown to result in less efficient bladder clearance of bacteria. We describe the use of a double antibody technique to semiquantitatively demonstrate the diminution of bladder glycoproteins from the transitional epithelium in oophorectomized rabbits. Rabbit bladder glycoprotein was isolated and used to immunize Swiss-Webster mice. Bladders of normal and oophorectomized rabbits were sequentially stained with mouse antirabbit sera and fluoresceinated goat antimouse antibody. A significant loss of bladder epithelial glycoprotein was evident in oophorectomized rabbits as compared to controls. This study, utilizing a semiquantitative immunologic staining technique, suggests an intact glycoprotein layer is important in the bladder defense mechanism.

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AnimalsCastrationEpitheliumFemaleFluorescent Antibody TechniqueGlycoproteinsMiceMucous MembraneOvaryRabbitsUrinary BladderJournal ArticleResearch Support, Non-U.S. Gov't

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K Weisman, H Callahan, H S Cooper, R W Fritz, S G Mulholland. 1984. Decrease in rabbit bladder mucosal glycoprotein after oophorectomy.. https://doi.org/10.1016/s0022-5347(17)49632-7

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