Dual signal-enhanced immunochromatographic test strip based on Au@PtNPs: From sensitive detection of thiamethoxam to multiplex pesticide screening in vegetables.

Zhengtao Li; Lingjun Geng; Jiashuai Sun; Jingcheng Huang; Rui Xu; Zhen Guo; Shouyi Dou; Liying Zhang; Fangling Du; Yemin Guo; Xia Sun; Nortoji A Khujamshukurov

doi:10.1016/j.foodchem.2026.150416

PubMed · 42462301

Dual signal-enhanced immunochromatographic test strip based on Au@PtNPs: From sensitive detection of thiamethoxam to multiplex pesticide screening in vegetables.

Zhengtao Li·Lingjun Geng·Jiashuai Sun·Jingcheng Huang·Rui Xu·Zhen Guo·Shouyi Dou·Liying Zhang·Fangling Du·Yemin Guo·Xia Sun·Nortoji A Khujamshukurov

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Abstract

Immunochromatographic test strip (ICTS) is a rapid analytical technique widely used in environmental and food detection owing to its merits of simple operation and short analysis time. Herein, three-dimensional nanoflower-structured gold‑platinum nanoparticles (Au@PtNPs) were synthesized via a seed-growth method. Compared with conventional gold nanoparticles (AuNPs), Au@PtNPs exhibited stronger signal intensity, excellent catalytic performance, and efficient antibody binding efficiency. Colorimetric Au@PtNPs-ICTS and catalytic colorimetric Au@PtNPs-ICTS were developed for the sensitive detection of thiamethoxam (THI) in vegetables. The limits of detection (LODs) for colorimetric Au@PtNPs-ICTS and catalytic colorimetric Au@PtNPs-ICTS quantitative analysis were 0.18 ng/mL and 0.093 ng/mL, respectively, representing approximately 3-fold and 6-fold improvement compared to AuNPs-ICTS (0.56 ng/mL). Furthermore, highly sensitive detection of multiple pesticide residues (chlorpyrifos, acetamiprid, and imidacloprid) was achieved by replacing the corresponding target antigens and antibodies, which further verified the universality of this immunochromatographic strategy.

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Thiamethoxam Vegetables Gold Metal Nanoparticles Food Contamination Neonicotinoids Chromatography, Affinity Platinum Pesticide Residues Limit of Detection Pesticides Immunoassay Nitro Compounds Enzyme-catalyzed signal amplification Immunochromatography Nanocomposites Journal Article Evaluation Study

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Zhengtao Li, Lingjun Geng, Jiashuai Sun, Jingcheng Huang, Rui Xu, Zhen Guo, Shouyi Dou, Liying Zhang, Fangling Du, Yemin Guo, Xia Sun, Nortoji A Khujamshukurov. 2026-07-14. Dual signal-enhanced immunochromatographic test strip based on Au@PtNPs: From sensitive detection of thiamethoxam to multiplex pesticide screening in vegetables.. https://doi.org/10.1016/j.foodchem.2026.150416

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