A point-of-use SERS assay for rapid detecting difenoconazole and flusilazole residues in fruit juices using Au/COF substrate.

Haitong Wang; Cunxiang Bo; Bingxin Xu; Yu Liu; Mengping Zhang; Xinyuan Zhao; Liqun Song; Hua Shao; Cuijuan Wang

doi:10.1016/j.foodchem.2026.150294

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A point-of-use SERS assay for rapid detecting difenoconazole and flusilazole residues in fruit juices using Au/COF substrate.

Haitong Wang·Cunxiang Bo·Bingxin Xu·Yu Liu·Mengping Zhang·Xinyuan Zhao·Liqun Song·Hua Shao·Cuijuan Wang

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Abstract

We developed a ready-to-use surface-enhanced Raman scattering (SERS) sensor for rapid, pretreatment-free detection of difenoconazole (DIF) and flusilazole (FLU) in peach and lychee juices. The substrate combines Au nanoparticles (AuNPs) with covalent organic frameworks (COF) and is implemented on a portable 25-well plate, enabling in situ testing. Juices can be directly applied to the SERS-active Au/COF composite, allowing simultaneous adsorption and signal generation. The correlation between SERS intensity and logarithmic concentration yielded R-values between 0.925 and 0.986, meeting the monitoring needs of non-laboratory scenarios. The entire workflow completes within 12 min, offering a faster alternative to conventional methods while maintaining high sensitivity and reproducibility. Detection limits reach 0.96-1.22 ppb for DIF and FLU, both of which are below the regulatory maximum residue limits. Distinct SERS fingerprints enable reliable discrimination of mixed residues across juice matrices, supporting rapid on-site monitoring and cost-effective pesticide surveillance.

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Triazoles Pesticide Residues Gold Spectrum Analysis, Raman Fruit and Vegetable Juices Food Contamination Metal Nanoparticles Dioxolanes Silanes Difenoconazole, Flusilazole Integrated functions On-site setting Ready-to-use Surface-enhanced Raman scattering Journal Article Evaluation Study

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Haitong Wang, Cunxiang Bo, Bingxin Xu, Yu Liu, Mengping Zhang, Xinyuan Zhao, Liqun Song, Hua Shao, Cuijuan Wang. 2026-07-06. A point-of-use SERS assay for rapid detecting difenoconazole and flusilazole residues in fruit juices using Au/COF substrate.. https://doi.org/10.1016/j.foodchem.2026.150294

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