Proteomic analysis of cisplatin-induced spermatogenesis defects in mice.

Pengyu Li; Ziran Chen; Zexuan Zhang; Hongbin Li; Yingnan Zhang

doi:10.1080/07853890.2026.2612816

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Proteomic analysis of cisplatin-induced spermatogenesis defects in mice.

Pengyu Li·Ziran Chen·Zexuan Zhang·Hongbin Li·Yingnan Zhang

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Abstract

BACKGROUND: Cisplatin is a crucial chemotherapeutic agent used for treating various cancers; however, its excessive use can cause irreversible damage to the reproductive system, and the protein expression profile of cisplatin-induced testicular injury remains unclear. METHODS: Male C57BL/6 mice were treated with cisplatin at various doses, and testes were collected for histological, immunofluorescence, and proteomic analyses. Germ cell loss and apoptosis were assessed using H&E staining, TUNEL assays, and immunofluorescence for LIN28A, SYCP3, MVH, and CDK1. Label-free quantitative proteomics identified differentially expressed proteins, which were analyzed for functional enrichment and protein-protein interactions. RESULTS: We observed that cisplatin treatment led to smaller testes, reduced sperm count, and a significant decrease in the number of spermatocytes and spermatids in mice. Label-free quantitative proteomic analysis revealed that cisplatin significantly reduced the expression of cyclin-dependent kinase 1 (CDK1), a key spermatogenesis regulator, in the testes. Reduction in CDK1 expression is correlated with spermatogenic arrest, particularly in spermatocytes. CONCLUSION: These findings highlight the critical role of CDK1 in cisplatin-induced spermatogenic dysfunction and provide new insights into fertility preservation strategies for patients with cancer undergoing chemotherapy.

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Animals Male Cisplatin Spermatogenesis Proteomics Mice Mice, Inbred C57BL Antineoplastic Agents Testis CDC2 Protein Kinase Apoptosis Sperm Count Spermatocytes CDK1 cisplatin spermatogenic injury Journal Article Research Support, Non-U.S. Gov't

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Pengyu Li, Ziran Chen, Zexuan Zhang, Hongbin Li, Yingnan Zhang. 2026-01-09. Proteomic analysis of cisplatin-induced spermatogenesis defects in mice.. https://doi.org/10.1080/07853890.2026.2612816

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Animals subject Antineoplastic Agents subject Apoptosis subject Journal Article subject Journal Article; Research Support, Non-U.S. Gov't resource Male subject Mice subject

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