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PubMed · 16299479

Fiber-optic fluorescence imaging.

Benjamin A Flusberg·Eric D Cocker·Wibool Piyawattanametha·Juergen C Jung·Eunice L M Cheung·Mark J Schnitzer
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Abstract

Optical fibers guide light between separate locations and enable new types of fluorescence imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes, flexible endoscopes well suited for imaging within hollow tissue cavities and microendoscopes that allow minimally invasive high-resolution imaging deep within tissue. A challenge in the creation of such devices is the design and integration of miniaturized optical and mechanical components. Until recently, fiber-based fluorescence imaging was mainly limited to epifluorescence and scanning confocal modalities. Two new classes of photonic crystal fiber facilitate ultrashort pulse delivery for fiber-optic two-photon fluorescence imaging. An upcoming generation of fluorescence imaging devices will be based on microfabricated device components.

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AnimalsFiber Optic TechnologyHumansMicroscopy, FluorescenceOptical FibersJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Review

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Benjamin A Flusberg, Eric D Cocker, Wibool Piyawattanametha, Juergen C Jung, Eunice L M Cheung, Mark J Schnitzer. 2005. Fiber-optic fluorescence imaging.. https://doi.org/10.1038/nmeth820

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