PubMed · 14051929
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J D WOFFORD. 1963. PROGRESSIVE SYSTEMIC SCLEROSIS.. https://pubmed.ncbi.nlm.nih.gov/14051929/
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A unique peptide sequence of HGGHHG screening from a combinatorial synthetic peptide library showed a good chelating ability to bind a transition metal on a chip better than hexa-His peptide. It was directly conjugated with a His-Tagged proteins onto a chip in a mild aqueous solution and can be used this chip as a high throughput technique for protein array in order to detect and purify the His-Tagged proteins.
BACKGROUND: Human chorionic gonadotropin (hCG) is among the most common analytes available for point-of-care immunotesting, with most assays currently based on simple manual assay devices. However, as the importance of good analytical performance of rapid assays is increasingly emphasized, more sophisticated immunoassay techniques are needed to meet the future challenges of rapid yet quantitative POC testing. METHODS: We developed a simple, dry-reagent, all-in-one immunoassay for the quantitative measurement of hCG in whole blood, plasma, or serum. The noncompetitive assay equally measures intact, nicked, and hyperglycosylated hCG as well as nonnicked and nicked hCG beta-subunit with a rapid and simple procedure consisting of a 5-min, one-step incubation and, subsequent to washing, the measurement of time-resolved fluorescence directly from a wet well surface. RESULTS: The assay had a detection limit (background + 3 SD) of 0.4 IU/L hCG. The within-run CV was <15% down to 2 IU/L, and the assay was linear to 6000 IU/L. The within- and between-run CVs in heparinized whole blood and plasma were </=10% throughout the measured range (4.0-4400 IU/L). The mean (95% confidence interval) difference between whole blood and plasma was -42 (-24 to -61)% without hematocrit correction and 6.5 (-14 to 27)% with hematocrit correction (n = 106). Regression analysis with the Diagnostic Products IMMULITE 2000 hCG method yielded the following: slope (SD), 1.02 (0.01); y-intercept (SD), -6 (10) IU/L; S(y|x) = 99 IU/L (n = 124; range, 1.6-4746 IU/L; r = 0.995). CONCLUSIONS: Combined with the fully automated instrumentation, the 5-min, dry-reagent assay allows quantitative and reproducible determination of hCG in whole blood while sustaining the speed and simplicity of conventional rapid assays.
Removal of Cu and Cr from chromated copper arsenate (CCA)-treated wood waste under batch electrodialytic conditions was studied. The effect of different types of extracting solutions, such as deionised water or aqueous solutions of NaCl, formic acid, oxalic acid, and EDTA, on the magnitude and direction of the fluxes of Cu- and Cr-containing species in the electrodialytic cell was investigated. Oxalic acid was found to have the best performance if simultaneous removal of the two elements is required (removal efficiencies of 80.5% for Cu and 87.4% for Cr, respectively). A mixture of oxalic acid and formic acid also led to similar removal efficiencies. In these experiments, the target elements were accumulated in both the anode and cathode compartments of the electrodialytic cell due to the formation of negatively charged complexes with the organic acids used besides the free cationic forms. The latter were not present if EDTA was the extracting solution resulting in directing the Cu and Cr fluxes to the anode compartment. Contrary, these fluxes were exclusively to the cathode compartment if deionised water or an aqueous solution of NaCl were used. These extracting solutions proved suitable for solubilising (re-mobilisation) of Cu but were less efficient for Cr removal (less than 20% removal). Overall, the results obtained show the important role of the proper selection of the type and composition of the extracting solution for the success of subsequent electrodialytic removal of Cu and Cr from CCA-treated wood waste.