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PubMed · 12877165

Immuno-based sample preparation for trace analysis.

Marie-Claire Hennion·Valérie Pichon
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Abstract

Immuno-based sample preparation techniques are based upon molecular recognition. Thanks to the high affinity and high selectivity of the antigen-antibody interaction, they have been shown to be a unique tool in the sampling area. Immuno-based sample preparation methods include the widely encountered immunoaffinity extraction sorbents, so-called immunosorbents, as well as membrane-baed or ultrafiltration techniques. This review describes the new developments and applications that have occurred in recent years with emphasis on (i) the antigen-antibody interactions, (ii) and their importance for the properties and use of immunosorbents, (iii) multiresidue extractions, (iv) the on-line coupling to chromatographic or electrophoretic separations, and (v) the high potential for improving MS detection. The recent use of artificial antibodies for sample pretreatment, so-called molecularly imprinted polymers, is also described.

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Antigen-Antibody ReactionsChromatography, GasElectrophoresis, CapillaryImmunoassayJournal ArticleReview

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Marie-Claire Hennion, Valérie Pichon. 2003-06-06. Immuno-based sample preparation for trace analysis.. https://doi.org/10.1016/s0021-9673(03)00529-6

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