Diffraction-based assay for detecting multiple analytes.

Betty Goh; Richard W Loo; Richard A McAloney; M Cynthia Goh

doi:10.1007/s00216-002-1478-5

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Diffraction-based assay for detecting multiple analytes.

Betty Goh·Richard W Loo·Richard A McAloney·M Cynthia Goh

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Abstract

The principles of diffraction are utilized to enable the simultaneous detection of multiple analytes in solution, forming the basis of a multi-analyte sensor. Probe molecules are immobilized on a substrate such that each type of molecule defines a specific pattern within the same region of substrate. The binding of a target molecule to its complementary probe is heralded by a characteristic diffraction image. This principle is demonstrated using antibody conjugates.

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Animals Antibody Specificity Avidin Biosensing Techniques Biotin Fluorescein Immunoglobulin G Light Mice Molecular Probes Protein Binding Rabbits Sensitivity and Specificity Solutions Substrate Specificity Journal Article Research Support, Non-U.S. Gov't

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Betty Goh, Richard W Loo, Richard A McAloney, M Cynthia Goh. 2002-08-10. Diffraction-based assay for detecting multiple analytes.. https://doi.org/10.1007/s00216-002-1478-5

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