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PubMed · 11572785

Conditional transgenic technologies.

A D Ryding·M G Sharp·J J Mullins
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Abstract

Transgenic technology has been revolutionised by the development of techniques that allow temporo-spatial control of gene deletion or expression in transgenic animals. The ability to switch gene expression 'on' or 'off' in restricted tissues at specific times allows unprecedented flexibility for exploring gene function in both health and disease. As use of these techniques grows in all areas of biomedical research, an understanding of this topic is essential. In this review we examine the theory, application and limitations of these strategies, with particular reference to endocrine research.

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AnimalsBacterial ProteinsBrainBreedingCytochrome P-450 CYP1A1EcdysoneEndocrinologyEnzyme InductionGene DeletionGene ExpressionGene Expression Regulation, EnzymologicGene Transfer TechniquesHumansIntegrasesLiverMiceMice, TransgenicModels, AnimalMutagenesis, Site-DirectedProtein Synthesis InhibitorsRecombination, GeneticResearchStem CellsTetracyclineViral ProteinsJournal ArticleResearch Support, Non-U.S. Gov'tReview

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A D Ryding, M G Sharp, J J Mullins. 2001. Conditional transgenic technologies.. https://doi.org/10.1677/joe.0.1710001

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