Surface-based DNA computing operations: DESTROY and READOUT.

L Wang; Q Liu; A G Frutos; S D Gillmor; A J Thiel; T C Strother; A E Condon; R M Corn; M G Lagally; L M Smith

doi:10.1016/s0303-2647(99)00046-5

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Surface-based DNA computing operations: DESTROY and READOUT.

L Wang·Q Liu·A G Frutos·S D Gillmor·A J Thiel·T C Strother·A E Condon·R M Corn·M G Lagally·L M Smith

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Abstract

DNA computing on surfaces is where complex combinatorial mixtures of DNA molecules are immobilized on a substrate and subsets are tagged and enzymatically modified (DESTROY) in repeated cycles of the DNA computation. A restriction enzyme has been chosen for the surface DESTROY operation. For the READOUT operation, both cycle sequencing and PCR amplification followed by addressed array hybridization were studied to determine the DNA sequences after the computations.

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L Wang, Q Liu, A G Frutos, S D Gillmor, A J Thiel, T C Strother, A E Condon, R M Corn, M G Lagally, L M Smith. 1999. Surface-based DNA computing operations: DESTROY and READOUT.. https://doi.org/10.1016/s0303-2647(99)00046-5

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