Biomedical subjects
Publications and source records attributed to Z Wu.
The Chinese medication Qizhi Weitong Powder has been proved very helpful in depressing gastric evacuation and small intestinal propel reducing gastric secretion, lowering acidity of gastric liquid and activity of pepsin as well as enhancing peristalsis of domestic rabbits. Other effects such as distinct protection to ligated ulcer of pylorus and painkilling have also been confirmed.
This paper reports the standards for microscopic quantitative analysis of Rhizoma Coptis. By comparing the microscopic quantitative analysis with spectrophotometry, the content of Rhizoma Coptis in Xianglian Pills has been determined and the accuracy of this method confirmed.
AIM: To evaluate biological effects in vitro of transforming growth factor-beta (TGF-beta) and/or insulin on proliferation of the human periodontal ligament (PDL) cells. METHODS: Cell cultures and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay. RESULT: TGF-beta at concentrations of 0.1 to 10 micrograms/L and insulin at 10 to 1,000 U/L had significant proliferative effects on human PDL cells (P < 0.01). From the 3rd day, the significant proliferation of PDL cells by TGF-beta or insulin were observed (P < 0.01) and lasted to the 5th day. The effect of combination of TGF-beta with insulin was the most significant one (P < 0.01). CONCLUSION: TGF-beta and/or insulin may be valuable in promoting the regeneration of periodontal connective tissue.
PURPOSE: Neuron-specific enolase (NSE) of containing gamma-enolase is considered valuable in the diagnosis of tumours of neuroectodermal origin. METHOD: We used rapid electrophoretic method on cellulose acetate plate to determine the pattern of enolase isoenzymes in 21 aqueous humor and 23 serum specimens from retinoblastoma (Rb) and 21 aqueous and 25 serum specimens from 25 control cases to evaluate NSE in the diagnosis of Rb. The assay allowed assessment of all three major isoenzymes (alpha alpha, alpha gamma and gamma gamma), and NSE relative activity and its percentage in the total relative activity of the three enolase isoenzymes were assessed by means of fluorometer. RESULT: Aqueous from all patients with Rb contained alpha alpha, alpha gamma and gamma gamma isoenzymes and presented strong positive, the positive rate of NSE being 100% and its relative activity accounting for 45 +/- 9% of the total relative activity of the 3 enolase isoenzymes; No enolase was detectable in control aqueous with cataract, glaucoma and Coats's diseases (4 cases), but in two patients with traumatic hyphema alpha alpha and faint alpha gamma and gamma gamma were presented in aqueous. The control serum contained only an alpha alpha band, while the sera form patients with Rb contained alpha alpha, alpha gamma and gamma gamma bands in 18 of 23 specimens, the positive rate being 78.2%, and only alpha alpha bands in the rest 5 specimens (21.8%). In patients with Rb, the NSE relative activity and its percentage in the total relative activity of the 3 isoenzymes in serum (36 +/- 9%) were significantly lower lever than those in aqueous. CONCLUSION: The amounts of NSE increase significantly in both serum and aqueous from patients with Rb, and the immunoelectrophoretic assay for determination of enolase patterns is valuable in the diagnosis of Rb and differential diagnosis of Rb. In addition, the method is rapid, simple and requires only a little amount (< 5 microL) of samples.
OBJECTIVE: To disclose the nature of RB1 germline mutations in Chinese and to develop a practical and effective way for mutational screening. METHODS: Leukocyte DNA was prepared from 8 Chinese patients with hereditary retinoblastoma. PCR combined with nonisotopic heteroduplex-SSCP analysis was used to screen leukocyte DNA for RB1 germline mutations, exon-by-exon, without the use of restriction endonuclease digestion. The mutations were finally identified by sequencing. In order to testify the effectiveness of this method, the same method was used to detect other 17 samples which have been previously analyzed by other methods. RESULTS: Heterozygous germline mutations were detected in the leukocyte DNA of 6 out of 8 Chinese patients: G del/codon 46, T del/codon 131, CAGAA del/codon 257-258, GCAgta-->GCAgca/donor of exon 16, C-->T/codon 661, and C-->T/codon 787. Heteroduplex-SSCP analysis may detect RB1 germline mutations in 68% (17/25) unselected patients, which is more effective than SSCP(56%) or heteroduplex analysis(64%) alone. The corrected RB1 mutation detection rate may be 80% if the samples were previously analyzed by Southern blotting, which is much higher than those reported on literature. CONCLUSION: Mutations involving a few base pairs in RB1 gene are common in Chinese. Heteroduplex-SSCP analysis is more useful and effective than SSCP or heteroduplex analysis alone for the rapid screening for unknown mutations.
PURPOSE: To evaluate Color Doppler Imaging (CDI) in the value of Persistent Hyperplastic Primary Vitreous(PHPV). METHODS: 3 cases (4 eyes) of PHPV were reproted and were examinated by high resolution CDI, the blood flow pattern was evaluated in basic of two dimensional grayscale image. RESULTS: CDI shows a band vascular structure coursing through the band-shaped moderately echogenic structure in the central portion of vitreous chamber, with the apex located near the optic disk and the base extending toward the lens. Spectral display of the Doppler signal indicated arterial flow. CONCLUSION: Central vessel exhibiting arterial flow is compatible with a prominent persistent hyaloid vascular structure. This CDI imaging feature, in addition to microphthalmia and lack of calcification, is virtually pathogenomonic of PHPV and aids in enabling distinction between PHPV and simulating lesions.
PURPOSE: To observe the effect of excimer laser photorefractive keratectomy (PRK) correcting myopia and astigmatism, and to study the related factors. METHOD: PRK was performed with the aim to correct myopia errors from 1.00 to 16.00 diopters on 529 myopia eyes (306 patients). All eyes were divided into two groups: Group I: -1.00-6.00 D(294 eyes); group II: -6.25-16.00 D(235 eyes). All the patients were followed up for 3 to 12 months. RESULTS: At postoperative 12 months the rates of uncorrected visual acuity more than or equal to 10/20 or 20/20 were 97.5%, 77.5% in group I, and 80.3%, 32.8% in group II respectively; 92.5% of eyes were within +/- 1.00 diopter of the desired emmetropia in group I, but 42.6% in group II; The influential factors of changes of postoperative diopters was the severity of corneal haze, methods of removing corneal epithelium, intraocular pressure, the central corneal thickness. CONCLUSION: The excimer laser PRK is regarded to be a very predictable, safe, stable, and effective method to correct myopia up to -6.00 D. Prior to operation, the plan would be disigned according to diopters, ages, corneal thickness intraocular pressure. During operation, the corneal epithelium bad better to be removed by laser.
TaA, the extracellular polysaccharide purified from the filtrate of fermented Tremella aurantia, is a water-soluble neutral polysaccharide. Gas chromatography of its alditol acetates has shown that TaA is composed of mannose and xylose by the ratio of 2.85:1. The physical and chemical properties of TaA have been characterized.
BACKGROUND: Human herpesvirus-6 (HHV-6) infection is ubiquitous in selected population with seroprevalence of 60-80%. Little is known for that in China, except few sporadic studies. To understand prevalence of HHV-6 antibody in Chinese population, this seroepidemiological study was conducted. METHODS: Sera were collected from 430 healthy persons and donors living in North China, and tested for HHV-6 antibodies using IFA with HHV-6 GS strain passaged on HSB-2 cells as antigen, and titer equal or higher than 1:10 was regarded as seropositive. RESULTS: Of the 430 serum samples tested, 297 (69.1%) were positive for HHV-6 IgG. Both seropositive rate and GMT in females were significantly higher than those in males, with X2 = 7.05, P < 0.01 and F = 7.23, P < 0.01, respectively. Statistically significant difference in prevalence of HHV-6 antibody among various age groups was observed in both sexes, with X2 = 20.08 and 20.28, P = 0.04, respectively, and young children had a higher titer than adults. But, no significant difference in prevalence was observed in blood donors between various age groups or both sexes. CONCLUSIONS: This study suggests that HHV-6 infection with seropositive IgG is ubiquitous in North China, and its importance should be further studied.
An automatic analyzer of RBC rheological parameters and a microscopic morphological observation technique were employed to investigate the effect of three electric field patterns on erythrocyte sedimentation and aggregation in a physiological saline solution and in autologous plasma, respectively. Experimental results indicate that in a physiological saline solution: 1) Erythrocytes present a lightly aggregation and settle faster by an alternative current electric field; 2) There exists no erythrocyte aggregation by a direct current electric field of constant current and the enhancement of erythrocyte sedimentation is caused only by the action of electric force; 3) Time courses of erythrocyte sedimentation fit well with an exponential type curve by a direct current electric field of constant voltage, and this exponential type sedimentation curve is not caused by erythrocyte aggregation but caused by the decrease of circuit current with time duration. Also shown by experimental results is that the above three electric field patterns all affect the erythrocyte sedimentation in antologous plasma in different contents, which predicts some change in erythrocyte aggregation behavior in autologous plasma by an external electric field.
This paper introduces a new apparatus and method that suits to measuring the water vapor transmission rate (WVTR) of wound covering materials. WVTR of synthetic materials, amniotic membrane, porcine and cadaveric skin was measured under different conditions of four temperatures, three mediums, and "water cup" or "inverted water cup". The temperature was in close relationship with the WVTR except Omiderm, amniotic membrane, and cadaveric skin between 30 degrees C and 37 degrees C groups. The medium did not affect the WVTR (P > 0.05). There was significant difference between the "water cup" and "inverted water cup" groups(P < 0.01). This study suggests that the apparatus is easy-to-operate, reliable and suitable for the measurement of WVTR of burn wound coverings and other membranous materials.
The component of the erythrocyte membrane protein under three states obstruction, after surgical relief of obstruction, and normal) were analyzed qualitatively by using SDS-PAGE and the method of thin scanning. The result revealed that the membrane protein band 4.2 was absent under the state of obstruction and it recovered after surgical relief of obstruction. Then the mechanical properties of the above three cases of erythrocytes were tested by the micropipette aspiration system and the data were analyzed by Voigt model. The result showed that the elastic modulus and the coefficient of viscosity of the erythrocytes under the state of obstruction were greater than those of the normal; thus the deformability decreased, but "overcorrection" appeared after surgical relief of obstruction. The possible relations between the mechanical properties and the change of membrane protein were discussed.
PURPOSE: To evaluate the use of ultrasound biomicroscope (UBM) in imaging anterior segment tumors of eye. METHODS: 13 cases of anterior segment tumors of eye were examined with UBM. RESULTS: 3 cases had the ocular history of trauma or surgery and were clinically diagnosed as anterior iris cysts. UBM displayed the cysts as thin-walled, no internal reflectivity, and at the locate of anterior humour angle; 8 cases had superficial anterior humour, narrow anterior angle and clinically be suspected as tumors behind iris or ciliary body. UBM showed cystic reflectivity in 7 cases and a solid tumor in ciliary body in one case. 2 cases were clinically diagnosed as iris nevi. UBM displayed solid convex lesions. CONCLUSION: UBM is a valuable new technique in the diagnosis and accurate localization of anterior segment tumors.
PURPOSE: To discuss clinic and histopathologic features of keratoacanthoma. The diagnosis and differential diagnosis were discussed. METHODS: The authors analyze clinical characteristics of keratoacanthoma which is testified by hisopathology. Clinically, the tumors developed rapidly. They appeared as a dome or nodule with a central keratin filled crater and elevated rolled margins. RESULTS: Histologically, they had a cup-differentiated squamous opithalium surrounding a central mass of keratin. The base was rather uniform. The lesions may extend into underlying stroma even orbicularis muscle. CONCLUSION: Keratoacanthoma is a specialized variant of pseudocarcinomatous hyperplasia. It has clinic and histopathologic characteristics and differs from other tumors.
This paper summarizes the "study on small hepatocellular carcinoma and its extension" in Liver Cancer Institute, Zhongshan Hospital of Shanghai Medical University during the past 25 years. The results indicated that it was an important approach to obtain long-term HCC survivors, of the 239 patients with 5-year survival, small HCC resection accounted for 51.4%. It was an effective approach to improve the prognosis of HCC in the entire series, the 5-year survival of inpatients treated in authors' institution was 4.8% in 1958-1970, 12.2% in 1971-1983, and 46.7% in 1984-1995; which were correlated to the increase proportion of small HCC resection in the series; it was more effective as compared to large HCC resection, the 5 year survival was 61.3% (n = 645) versus 33.6% (n = 950). Extensions of small HCC study included early detection and treatment of small recurrent HCC, of the 147 patients with re-resection, the 5-year survival was 48.9% calculated from the time of first resection. Another extension was conversion of large HCC into small HCC, using multimodality combination treatment, 72 out of the 663 patients with surgically verified unresectable HCCs have been converted to resectable, 5-year survival being 62.1%, which was comparable to that of small HCC resection. Studies on related basic aspect of small HCC such as cell origin of recurrence, and molecular aspect of small HCC, indicated that biological characteristics, particularly the tumor invasiveness, remained the key link for further prolong survival after small HCC resection. Recently, a "patient-like" human HCC metastatic model in nude mice has been established. Experimental interventions have also been tried. Clinical trials for prevention of recurrence after small HCC resection have shown preliminary encouraging results. However, the "cost-effectiveness" of screening, the invasiveness of HCC, the multicentric origin, the coexisted Child C cirrhosis, etc., remained great challenge.
To study the effect of hypertonic saline solution on the rheology and putative mechanism, we investigated the changes of plasma viscosity, blood viscosity and reduced viscosity in dogs treated with hypertonic saline solution after burn-blast combined injury and observed the effect of hypertonic saline solution therapy on the viscoelastic property of erythrocyte membranes measured by micropippette aspiration technique in rats with burn. The results showed that the blood viscosity and reduced viscosity increased significantly in dogs after injury, and plasma viscosity also increased significantly at 24 h after injury. Elastic moduli and viscous coefficients of erythrocyte membranes increased obviously in rats after burn. The hypertonic saline solution therapy could significantly improve blood viscosity and reduced viscosity, but it did not significantly improve elastic moduli and viscous coefficients of erythrocyte membranes. These suggested that the hardness of erythrocyte membranes increased, the deform property of erythrocyte membranes decreased, and the blood rheology became worse after burn blast combined injury. Hypertonic saline solution therapy could significantly improve the blood rheology. The effect did not bear a significant relationship with the change of single biomechanics property of erythrocyte membranes. It might be related with other factors.
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Cholagogic capsule is mainly composed of Fiuctus Cardui, Mariae, Oleum Menthae Piperilae, Radix Currmae, olia taraxaci, Oleum Hyperici. It is shown that Cholagogic Capsule increase the rat's bile secretion significantly, but bile component is not influenced effectively, the retention jaundice models of which are created by ANIT isn't influenced effectively. It can reduce the mouse pinna swelling caused by dimethythenzene and depress the metatarsal swelling induced by carragenin, the antiinflammatory effect keeps for more than 4 h. It inhibits the contraction of gallbladder smooth musele of Guinea Pig caused by Ach. The results show that cholagogic Capsule increase bile secretion, antispasmodic and antiinflammatory effect. It is suggested the therapeutic effects of Cholagogic Capsule in sand-like calcul and acute chronic cholecystitis are related to the above pharmaological effects.