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Biomedical subjects

Z Wu

Publications and source records attributed to Z Wu.

At least 487 records · Page 27Linked to original sources
PubMed1997

[Photorefractive keratectomy to correct myopic astigmatism].

The VISX 20/20 excimer laser was used to treat 260 eyes with myopia and myopic astigmatism of 166 patients. All patients were followed up for one year. The diopter of preoperative astigmatism was from -0.25-(- 4.00 D (-1.00 +/- 0.62 D). The diopter was lowered to -0.07 +/- 0.40 D at 6 months postoperatively (P < 0.05) and -0.06 +/- 0.39 D at 12 months postoperatively (P < 0.01). Photorefractive keratectomy (PRK) is effective and safe for myopic astigmatism.

Adolescent↗
PubMed1997

[Effects of endothelin-1 on the progesterone production of rat preovulatory ovarian granulosa cells].

The effect of endothelin-1 (ET) on progesterone production of isolated rat granulosa cells and its mechanism were investigated. ET could inhibit the hCG-stimulated progesterone production significantly at the concentration of 10(-8) mol/L (P < 0.05, n = 6) and highly significantly at the concentration of 10(-7) mol/L (P < 0.05, n = 6), but ET did not inhibit the basal progesterone production at various concentrations (10(-10)-10(-7) mol/L). Further experiments showed that the inhibitory effect of ET on the hCG-stimulated progesterone production could be reversed by rabbit antiserum to ET (ET-A, 1:1000) or cAMP (10(5) mol/L). ET could decrease the binding sites and the affinity constant (ka) of LH/hCG receptor on granulosa cells. All these findings suggest that ET may be an intraovarian regulatory factor which may inhibit progesterone production stimulated by hCG from rat granulosa cells through inerfering LH/hCG receptor function and cAMP formation.

Animals↗
PubMed1997

[Clinical significance of residual urine volume in bladder outlet obstruction with benign prostatic hyperplasia].

Bladder outlet obstruction (BOO) with benign prostatic hyperplasia (BPH) was determined by pressure-flow studies. Residual urine volume was measured by urethral catheterization. Fifty-one patients with or without BOO were investigated for residual urine. The mean residual urine volume was 32.8 +/- 35.9 ml and 25.2 +/- 22.7 ml in BOO group (31 patients) and no BOO group (20) respectively. The range of residual urine volume was from 6 to 210 ml and from 3 to 88 ml in the two groups respectively. Residual urine volume was not statistically different between the BOO group and no BOO group (P > 0.05). The results of pressure-flow studies were significantly different between the BOO group and no BOO group (P < 0.01). Residual urine determination was not a reliable criterion for selection of patients for surgery. The cause of residual urine is not only BOO. The patients with BOO may present insignificant residual urine. It is evident that the absence of post-void residual urine does not rule out BOO.

Aged↗
PubMed1997

[Color Doppler ultrasonography in evaluation of intraocular lesions].

OBJECTIVE: To evaluate the value of color Doppler ultrasonography (CDU) in the diagnosis of intraocular lesions. METHODS: 82 cases of intraocular diseases were examined by high-resolution CDU (7.5 mHz), and the blood flow pattern was evaluated at the base of two-dimensional image. RESULTS: CDU might show the blood flow of retinal vessels in all 21 cases of retinal detachment, no blood flow could be detected in 21 of 22 vitreous hemorrhages and/or membranes, and only in one case with diabetic proliferative retinopathy did fine blood flow be seen. Intratumor blood flow with branchy pattern could be detected in 17 of 18 cases with choroidal melanomas and 3 of 4 cases with choroidal metastatic carcinoma, and spectral analysis revealed high-resistance, simulating arterial blood flow waves. In 5 cases with choroidal hemangioma, intratumor blood flow with filled or sparkled pattern was detected, and the spectral analysis revealed both arterial and venous blood flow waves. No blood flow in the lesion was detected in simulating benign lesions i.e. 5 choroidal hemorrhages and 2 subretinal hemorrhages. In 3 cases (4 eyes) with persistent hyperplastic primary vitreous (PHPV), CDU showed band-shaped arterial blood flow coursing from optic nerve disc to the posterior surface of the lens in the vitreous cavity. CONCLUSION: CDU is helpful in the differential diagnosis among retinal detachment, vitreous hemorrhage, vitreous membrane, malignant choroidal melanoma, choroidal metastatic tumor, choroidal benign lesion or hemangioma, PHPV and lesions with white pupil.

Adolescent↗
PubMed1997

[The use of "self-locking" artificial vertebral prosthesis in surgical treatment for spinal tumor].

OBJECTIVE: To evaluate the use of "self-locking" artificial vertebral prosthesis in surgical treatment for spinal tumor. METHODS: From 1992 to 1996, 14 cases with spinal tumor and paraplegia were treated with "self locking" artificial vertebral prosthesis designed by one of the authors (Ye Qibin) in our department. There were malignant giant cell tumor 2 cases; myeloma 4 cases; metastatic tumor 4 cases and one each with chondrosarcoma, malignant fibrous histocytoma, fibrosarcoma, hemangioma. After radical resection of the tumor and thorough decompression, the vertebral prosthesis was applied to reconstruct the spinal stability. Adjunctive chemotherapy or/and radiotherapy was given postoperatively. RESULTS: At the average follow-up period of 20.6 months 10 cases are still alive, 5 of them had survived for more than 2 years. 4 cases died with the average survival period of 19.8 months after operation. Almost all the patients obtained the improvement of the quality of the life with relative pain-free and intact neurologic function after operation. CONCLUSION: With "self-locking" artificial vertebral prosthesis after thorough anterior resection of spinal tumor, it is possible to provide solid internal fixation to reconstruct the spinal stability.

Adult↗
PubMed1997

[Liver resection after transcatheter hepatic arterial chemoembolization for hepatocellular carcinoma and curative effect analysis].

To study the therapeutic result of hepatic resection for those hepatocellular carcinoma (HCC) shrunked after transcatheter hepatic arterial chemoembolization (TACE) in the patients with unresectable HCC, authors reported 59 patients with HCC. Among the 59 patients, the maximum diameter of the tumor was 5.6 to 20.0 cm prior to the first TACE, mean 9.43 cm. The patients underwent 1 to 6 times of TACE, mean 2.9 times. The tumor diameters were reduced to 3.29 cm prior to operations. The duration between the last TACE treatment and sequential resection varied from 1 to 7 months, mean 2.53 months. Of the 59 patients, 35 patients' serum alpha-fetoprotein (AFP) levels were elevated. AFP levels returned to normal after TACE treatment in 13 patients. Of the patients, liver segmentectomy, combined liver segmentectomy or partial liver resection was performed in 56 patients, left trilobectomy in 2 and left hemihepatectomy in 1. Tumor necrosis ranged from 40% to 100% pathologically and complete tumor necrosis occurred in 9 patients. Of the 13 patients with AFP levels decreased to normal, 9 still had microscopic living tumor foci. The 1-, 3- and 5- year survival rates were 79.7%, 65% and 56%, respectively. These results indicated that TACE treatment can provide chance of tumor resection for those patients with unresectable HCC and good results can be obtained.

Adult↗
PubMed1997

[Diagnostic value of CD44 splice variants in urine exfoliated cells of bladder cancer].

To study the early and non-invasive diagnostic value of CD44 splice variants in urine exfoliated cells of bladder cancer. We used reverse transcriptionpolymerase chain resection (RT-PCR) and southern blot hybridization to detect CD44 splice variants in exfoliated cells in 40 urine samples (20 bladder cancers, 20 non-neoplastic controls), and compared with the results of urine cytology on the same set of samples. 90% (18/20) of the urine samples of bladder cancer showed overexpression of CD44 splice variants while none of the 20 controls did so. This method not only has a sensitivity of 90% (18/20) which was much higher than that of 65% (13/20) by using urine cytology, but also is non-invasive and comfortable. The results suggest that CD44 splice variants in exfoliated cells in urine samples are a new tumor marker for early and non-invasive diagnosis of bladder cancer.

Biomarkers, Tumor↗
PubMed1997

[Nutritional effects of glutamine-enriched parenteral nutrition on transplanted small intestine in the rat].

We evaluated the preventive effects of glutamine-enriched pareneral nutrition on the atrophy and hypofunction of the transplanted small intestines in the rat. Wistar rats received jejunal isografts and central venous catheters for parenteral nutrition (PN) for ten days. Rats received either PN with 3% alanyl-glutamine or the PN with isonitrogenous ballanced nonessential amino acids. Glutamine-enriched PN significantly increased mucosal villous height mucosal thickness, crypt depth and villous surface area compared with the non-glutamine PN. Normal enterocyte ultrastructure of the graft was maintained in the Glutamine-enriched group. Atrophied microvilli and broken mitochondrial crista were observed in the control group. It was found that the amount of absorption of 15N-glycine was greater at the 1st, 2nd, 3rd hour in glutamine-enriched group than that in the control. These results indicate that the glutamine-enriched parenteral nutrition can promote the proliferation of mucosa, maintain the ultrastructure and improve the amino acid absorption in the transplanted small bowel in the rat.

Animals↗
PubMed1997

[DNA polymorphisms of apolipoprotein A I gene in Chinese endogenous hypertriglyceridemics].

The restriction fragment length polymorphisms (RFLPs) of apolipoprotein(apo) A I gene were studied using polymerase chain reaction (PCR) in 69 endogenous hypertriglyceridemics (HTG) and 74 healthy subjects from a population of Chinese Han nationality in Chengdu area. The loci studied included Msp 1 within intron 3 of the apo A I gene; Xmn I, 5' to the apo A I gene; and Pst I, 3' to the apo A I gene. The results showed that both in HTG group and control group M1, X1 and P1 alleles were the major alleles and homozyous M1M1, X1X1 and P1P1 genotypes were the most frequent ones. The frequencies of rare M2 and X2 alleles in Chinese were significantly higher than those in European Caucasians (0.293 vs 0.111, P < 0.0006, 0.286 vs 0.130, P < 0.004), but no differences were found in the frequency of rare P2 allele (0.071 vs 0.046, P > 0.05). The frequency of rare M2 tended to increase in the HTG group when compared with the control group (0.353 vs 0.275, P > 0.05). Both in the HTG group and control group, subjects with genotype M2M2 had a higher serum mean concentration of TC, apo C II and higher serum TG/HDL-C ratio compared with the subjects with the genotypes M1M1 and M1M2 (P = 0.05, P < 0.03, P < 0.04), and the serum TG, apo C II and apo E levels had a tendency to increase. The subjects with the genotypes P1P2 and P2P2 had a higher serum apo A II levels compared with the subjects with the genotype P1P1 (P < 0.04). The data show that the only Msp I RFLP within the intron 3 of apo A I gene is associated with endogenous hypertriglyceridemia to some extent in Chinese population.

Adult↗
PubMed1997

[The serum lipid and apolipoprotein levels of middle-aged male hyperlipidemics in Chengdu district].

It has been evidenced that cardiovascular diseases (CVD) relate with many risk factors and serum lipids play and essential role in the development of CVD. In order to further study hyperlipidemia (HL) in the middle-aged males, we analysed the body mass index (BMI), fast blood sugar (FBS), serum lipids and apolipoproteins A I, A II, B100, C II, C III and E in 223 male HL patients aged 41-60 and 349 normal male subjects who matched the HL patients in age. The result showed that the increase of serum triglycerids (TG), was as might be expected, the major characteristic of the middle-aged male HL patients in Chengdu District. Besides the serum lipid and apolipoprotein B100, C II, C III and E levels, the BMI and FBS levels in the IIb, IV and V types of HL patients were significantly higher than those in the normal subjects. It also showed that in TG increased patients the increased percentages of serum lipids were significantly higher than those of apolipoproteins. The age distribution in HL patients revealed that only 25% of the HL patients were between 41 to 50 years old, while 50% in the 56-60 year-old group. The relationship between apolipoprotein levels and serum lipid metabolism is also discussed. The results suggest that the 51-60 year-old males should pay attention to diet and increase physical activities to reduce incidence of HL which is directly associated with CVD.

Adult↗
PubMed1997

[Relation of serum apolipoprotein B100 levels to lipids and apolipoproteins A I, A II, C II, C III and E levels in the middle and old age normal males in Chengdu area].

This study was conducted in 438 normal men aged 40-70. The age groups were: 40-44, 45-49, 50-54, 55-59, 60-64, and 65-70. The results showed that the serum apolipoproteins levels (mean+/- s, mg/L) were: B100 levels 742.5 +/- 165.8, A I 1301.2 +/- 219.9, A II 299.4 +/- 49.0, C II 42.7 +/- 21.4, C III 113.3 +/- 34.0 and E 38.7 +/- 8.2. The levels of serum apoB100 increased with age. The serum apoB100 levels in the 5th and 6th age groups were significantly higher as compared with the groups 1, 2, 3 and 4 (P < 0.05 and P < 0.05). The fasting serum mean TG (1.39 +/- 0.45 mmol/L), TC(4.79 +/- 0.82 mmol/L) and LDC-C(1.62 +/- 0.40 mmol/L levels in the groups 5 and 6 were significantly higher as compared with the groups 1, 2, 3 and 4 (P < 0.01). Serum apoC II, C III and E levels were significantly increased in the groups 5, 6 (P < 0.05). The correlation analysis indicated that there was a positive correlation of apoB100 with serum TG, TC, LDL-C, apoC II, C III and E respectively (P < 0.01); and a negative correlation with HDL-C levels (r = 0.1312); and apoB100 correlated negatively with apoA I (r = -0.0706). The results suggest that serum TG, TC, LDL-C, apoC II, C III and E are the main factors related with the serum apoB100 levels.

Adult↗
PubMed1997

[Effects of chronic hypoxia on calmodulin activity in rats].

To investigate the effects of chronic hypoxia of calmodulin activity in rats, we divided 40 wistar rats into four major groups. One group acted as control. The other three groups were made hypoxic by placing them in an isobaric hypoxic chamber (O2 = 10%) for 1, 2 and 3 weeks respectively. Calmodulin activity was measured by Phosphodiesterase method. The results showed that the mean pulmonary arterial pressure and the pulmonary vascular resistance in rats were remarkably increased by chronic hypoxia. The total calcium levels and the calmodulin content in the lung tissue of rats were significantly elevated at 2-3 weeks of hypoxia exposure. However, the calmodulin content in the lung tissue of the rats exposed to hypoxia for 1 week also increased, but it was lower than those at 2-3 weeks of hypoxia exposure. Therefore, we conclude that Ca(2+)-calmodulin system may play a central role in mediating hypoxai-induced pulmonary hypertension.

Animals↗
PubMed1997

[Quantitation of human apolipoprotein B100 by immunoturbidimetric assay].

An immunoturbidimetric assay(ITA) for quantification of human serum apolipoprotein B100 (apoB100) was developed. The assay is sensitive, rapid, specific, easily automatic and thus convenient for routine work. The minimu measurable concentration of apoB100 was 3.1 micrograms in each assay. The standard curve with a working range of 0.34-1.79 g/L was plotted. The between and within assay coefficients of variation were 1.13%-3.48% and 2.91%-4.48% respectively. The recovery was 99.23% +/- 3.57%. The mean serum concentration of apoB100 in 100 normal subjects was 0.821 +/- 0.165 g/L. The correlation coefficient of RID for apoB100 to ITA was 0.8626(P < 0.001).

Apolipoprotein B-100↗
PubMed1997

[The expression of mdm2 gene related to the invasiveness of human hepatocellular carcinoma].

OBJECTIVE: mdm2 gene was used as an index to study the molecular mechanism related to the invasiveness of human hepatocellular carcinoma (HCC). METHODS: The expression of mdm2 gene in 34 HCC samples and 19 paratumor liver tissues and the relationship between the expression of mdm2 gene and invasiveness of HCC were studied using reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: The expression level of mdm2 gene (x +/- Sx) in HCC samples was higher than that in paratumor liver tissues (50.18% +/- 5.24% vs. 27.70% +/- 7.43%, P < 0.05). The expression level of mdm2 gene in HCC samples with invasiveness was higher than that in HCC samples without invasiveness (59.09% +/- 7.28% Vs. 37.87% +/- 6.37%, P < 0.05). However, significant difference in expression of mdm2 gene was not found between small and large HCC as well as between HCC with and without capsule. CONCLUSION: The expression of mdm2 gene was related to the invasiveness of HCC.

Carcinoma, Hepatocellular↗
PubMed1997

[Comparative studies on pharmacological and toxic actions of raw fructus Psoraleae and its salt-baked and improved salt-baked products].

The pharmacological and toxic actions of raw Fructus Psoraleae and its salt-baked, and improved salt-baked products were comparatively studied. The leucogenic action of the improved salt-baked product was more effective than that of the original salt-baked one. But no significant difference in antidiarrheal or antiandrogenoid effect was found between the two salt-baked products. The toxicity(LD50 and the injury on kidney) of the improved salt-baked product was lower than that of the salt-baked. one. The results indicate that this improved processing method would contribute to increasing the efficacy and decreasing the toxicity of Fructus Psoraleae.

Androgen Antagonists↗