Biomedical subjects

Z Wang

Publications and source records attributed to Z Wang.

At least 811 records · Page 45Linked to original sources

PubMed2000

[Lymph node metastasis and the extent of lymph node dissection for gastric cancer: report of 326 cases].

OBJECTIVE: To investigate the pattern of lymph node metastasis and the extent of lymph node dissection for gastric cancer. METHODS: 326 patients with gastric cancer admitted from 1990 to 1999 were analyzed retrospectively after D(2), D(3) or D(3) plus para-aortic lymphadenectomy (D(3) + PAL). RESULTS: The total incidence of lymph node metastasis was 69.9%; node involvement was 15.4% and 77.4% respectively for early gastric cancer and advanced gastric cancer. Depth of invasion, tumor size and histology affected lymph node metastasis significantly (P < 0.05). For T(1) patients, node involvement was mainly confined to N(1) and one patient had N(2) metastasis; 8.1% of T(2) patients and 28.7% of T(3), T(4) patients had N(3), M(1) lymph node metastasis. Among 107 patients who received D(3) + PAL, 16a2b1 lymph node metastasis was found in 15.0%. The patients who had 16a2b1 node involvement were all suffered from advanced gastric cancer and N(1)-N(2) node metastasis. In the patients with serosal invasion-positive tumors or tumors size over 5 cm, the incidence of 16a2b1 metastasis was high, and that of entire stomach cancer was up to 38.5%. The 3-year and 5-year survival rates for D(3) + PAL group were 60.7% and 50.0% respectively. After D(3) + PAL, the 1-and 2-year survival rate, of patients with 16a2b1 metastasis were 60.0%, 50.0% respectively. CONCLUSIONS: D(2) lymphadenectomy should be taken for patients with early gastric cancer and D(3) lymphadenectomy for those with relatively early' advanced gastric cancer. For advanced gastric cancer with suspect or confirmed N(1)-N(2) node metastasis, tumor size over 5 cm and/or serosa invasion, D(3) plus para-aortic lymphadenectomy appears to be a necessary surgical procedure.

Adult↗

PubMed2000

[Vitrectomy for pseudophakic retinal detachment].

OBJECTIVE: To study the method of vitrectomy for treatment of pseudophakic retinal detachment. METHODS: 32 pseudophakic retinal detachment eyes were treated with vitrectomy, primary vitrectomy in 11 eyes (34.4%), vitrectomy after conventional buckling failure in 21 eyes (65.6%), combined with silicone oil 10 eyes (31.3%), removal of intraocular lens 12 eyes (37.5%). All eyes had passed the 6 months to 5 years of follow-up examination. RESULTS: Intra-operative new retinal breaks were identified in 7 eyes (21.9%). The retinal total reattachment was in 25 eyes (78.1%); part reattachment was in 4 eyes (12.5%), and their visual acuity was improved than that before the operation. Due to severe proliferative vitreoretinopathy (PVR), failure occurred in 3 eyes. CONCLUSIONS: The main advantage of vitrectomy is the better intra-operative sight to the peripheral retinal breaks, removal of retinal traction and the low rate of PVR after vitrectomy. Vitrectomy for complicated type of pseudophakic retinal detachment can improve the success rate of the retinal detachment surgery and the visual acuity outcome.

Adult↗

PubMed2000

[MRI features of confusable tumors of orbital apex].

OBJECTIVE: To analyze magnetic resonance imaging (MRI) features of confusable tumors including cavernous hemangioma, cavernous lymphangioma and neurilemmoma located at orbital apex, which could provide evidences for diagnosis and surgical treatment. METHODS: MRI was performed on 16 cases with tumors of orbital apex confirmed by pathology, and their MRI findings were analyzed. RESULTS: The time taken to attain complete enhancement after contrast on MRI was significantly different in the three types of tumor (P < 0.01) Their enhancement patterns were related to their pathologic findings. CONCLUSIONS: The different enhancement patterns of various tumors of orbital apex are characteristic for diagnosis and differential diagnosis.

Adolescent↗

PubMed2000

[The recognition of the epidemic area of tsutsugamushi disease on Nan Peng Lie Island in China and the strategy of prevention].

OBJECTIVE: To investigate the region for the epidemic area of tsutsugamushi disease. METHODS: Epidemiological studies, Orientia tsutsugamushi isolation, and preventive measures were used. RESULTS: The region belonged to epidemic area of south subtropical zone. The main host was found Rattus norvegicu. The main biological vector was Leptotrombidium deliens. The seasonal trends of the quantity of Rattus norvegicu and Leptotrombidium deliens were consistent with the incidence change of the disease in the region. Orientia tsutsugamushi has been isolated from Rattus norvegicu and Leptotrombidium deliens. Data showed that Orientia tsutsugamushi isolated strains mainly belongs to Karp. Sero-epidemiological data showed that antibodies of Orientia tsutsugamushi are 100.0% positive in the residents of the region and 4.0% in army men. After taking comprehensive preventive measures, the incidence was descending. CONCLUSION: Nan Peng Lie island was proved an epidemic area of tsutsugamushi disease.

Animals↗

PubMed2000

[The epidemiological study of HIV infection among paid blood donors in one county of China].

OBJECTIVE: To observe the prevalence of HIV infection among paid blood donors and risk factors. METHODS: Selected the residents between 18 to 50 years old from two villages in one county of China in 1997. We observed the prevalence of HIV infection and keep following them to 2000. RESULTS: Nine hundred sixty-three residents were investigated and the prevalence of HIV was 1.8% (17/963). There were 88 paid blood donors among 963 interviewed subjects, the HIV prevalence was significantly higher (17.0% or 15/88) than those who didn't have the experiences of paid blood donations (0.2% or 2/875). Only one person out of 668 who were followed from 1997 to 2000 has converted to sero-positive, the incidence of HIV infection is 0.06 per 100 person-years. Nearly half of sero-negative and sero-positive people have the experiences of muscle injection. Among those with HIV-positive spouse, we found no one converted to sero-positive. The CD(4) count of those sero-positive people declined every year. HIV subtype and C2-V3 region sequence analysis were made for five HIV-positive people in 1998, the result indicated that all of them were infected with HIV-1 subtype B' and the genetic divergence rate was 2.4%. CONCLUSION: There was a high prevalence of HIV among paid blood donors in some regions, and paid blood (plasma) donations were the main risk factor for HIV infection. Although no evidence suggests that during the follow-up period the epidemic of HIV infection has spread locally, it still shall take measurements to prevent further transmission of HIV through paid blood donors.

Adolescent↗

PubMed2000

[Analysis on the intervention effect of HIV/AIDS cases].

OBJECTIVE: To study the effect of a comprehensive intervention strategy on the HIV infected individuals and their families. METHODS: Eleven HIV positive persons and their families were included to a comprehensive intervention strategy including routine surveillance, safer sex education and improving the status of hygiene. The outcome was compared with 2 fully developed AIDS cases who were not included to the intervention strategy. RESULTS: Of those HIV positive persons, that subjected to the intervention strategy, none had developed to AIDS, with only 1 transmitted HIV to his spouse among the 6 married individuals. All of their families were stable. Of those that not had been subjected, 2 individuals developed to AIDS and transmission in family was confirmed. CONCLUSION: The comprehensive intervention strategy on HIV/AIDS was effective which could the possibility of HIV in-family transmission and enhance the quality of life and the stability of their families.

Acquired Immunodeficiency Syndrome↗

PubMed2000

[A study on relationship between plasma heat stress protein 70 and cytokine in patients with heat apoplexy].

OBJECTIVE: To study the change of levels of plasma heat stress protein 70 (HSP70) and cytokine and their interrelationship in pathogenicity of heat apoplexy. METHODS: Plasma HSP70 was determined with Western blot, and plasma interleukin-2 (IL-2), soluble interleukin-2 receptor (sIL-2R) and interleukin-6 (IL-6) were determined with enzyme-linked immunosorbent assay (ELISA). RESULTS: Plasma level of HSP70 was 4 211.2 +/- 1,286.2 (integral optical density) in average in the patients of heat apoplexy group, 4,137.8 +/- 1, 207.5 in those of severe heat apoplexy group, and 6,043.5 +/- 1,354.8 in control group, with very significant difference (P < 0.01). Plasma IL-2 level was (64.3 +/- 32.3) pg/ml and (200.0 +/- 50.0) pg/ml and sIL-2R (54.9 +/- 33.3) U/ml and (167.5 +/- 89.0) U/ml in the patients with heat apoplexy and in controls, respectively, with very significant difference. But, there was no significant difference in IL-6 between varied groups. CONCLUSIONS: The above-mentioned results suggest that establishment of heat acclimatization correlates to the interaction of network of neuroendocrine-cytokine-HSP, in which decrease in plasma IL-2 was one of the important factors causing reduction of HSP70 expression. Balance of network regulation was broken by pathological factors, finally resulting in heat apoplexy.

Adolescent↗

PubMed2000

[Detection of C gene mutation strain in vertical transmission of hepatitis B virus and its significance].

OBJECTIVE: This study aims at detecting C gene mutation strain in vertical transmission of hepatitis B virus (HBV). METHODS: HBV C gene nt 2,022 - 2,321 was directly sequenced. RESULTS: Homology of HBV nucleotide sequence and amino acid sequence in mothers, fathers and their fetus was 100%. Mutation of 2,189 and 2,288 nucleotide of C gene resulted in substitution at the 97th and 130th amino acids. CONCLUSION: C gene mutation strain is present in vertical transmission of HBV. Presence of mutation strain may be the cause of failure in vaccination.

Adult↗

PubMed2000

[Characteristics of producing-fumonisin and dimorphic fungus of fusarium moniliforme].

OBJECTIVE: To study the carcinogenesis of fusarium moniliforme and its fumonisins in animals. METHODS: The producing-fumonisin and dimorphic fungus pathogenicity of F. moniliforme from Zhejiang, China were identified and characterized. RESULTS: The F. moniliforme isolated from foodstuffs was shown to produces fumonisin B(1) > 400 microg/g in medium by IC-ELISA. Ten foodstuffs samples showed (2% - 100%) detection rate of F. moniliforme and contained fumonisin B(1) between (3.7 - 143.1) microg/g. The strains of F. moniliforme formed yeast-like colony in Sabouraud's agar plates contained 9% NaCl at 37degrees C incubator and budding multiplication mostly. In blood agar plates the strains of F. moniliforme showed grass green haemolytic reactions. CONCLUSION: It is important to carry out (or to perform) oulepidemiologicol survey of F. moniliforme as a primary pathogenic fungus, as well as the mycotoxins of F. moniliforme in vitro.

Carcinogens, Environmental↗

PubMed2000

[Genetic epidemiological study on non-insulin dependent diabetes mellitus].

OBJECTIVE: To study the general genetic pattern of non-insulin dependent diabetes mellitus patients. METHODS: 1,608 children were investigated for NIDDM family history, and 280 nuclear families were collected. RESULTS: The prevalence rates of NIDDM among first-degree relatives (2.38%), the parents (26.00%), the siblings (2.44%) and the offsprings (1.24%) were higher than that in general population respectively. The s/q was 10.17 by Penrose method, which was close to 1/q(1/2). The p(0) was 0.0244 by simple segregation analysis, which was lower than 0.10. The heritability of NIDDM was 0.54, using the Falconer Threshold Model. CONCLUSION: NIDDM has a familial aggregation, but not fit to the mono-genetic models. NIDDM has the feature of multifactorial inheritance.

Adult↗

PubMed2000

[p16, p53 and c-erbB-2 gene expression in bladder carcinoma].

OBJECTIVE: To investigate the expression of p16, p53 and c-erbB-2 proteins in bladder carcinoma and to analyze their correlation with tumor grade, clinic stage and metastasis. METHODS: Immunohistochemical method was used to detect the p16, p53 and c-erbB-2 gene expressions in 75 cases of bladder carcinoma. RESULTS: Positive rates of p16, p53 and c-erbB-2 in 75 cases of bladder cancer were 41.3%, 44.0% and 40.0%, respectively. p16 and c-erbB-2 gene expressions were related to the tumor grade and clinic stage (P < 0.05); p53 and c-erbB-2 gene expressions were related to the clinic stage and metastasis (P < 0.01). Oncogenes and/or antioncogenes altered expressions were found in 77.3% cases of bladder carcinoma, and oncogenes and/or antioncogenes with multiple altered expressions were found in 53.3% cases. CONCLUSION: The analysis of multiple oncogene and/or antioncogene is more valuable than that of single oncogene and/or antioncogene. The altered expression of oncogene c-erbB-2 and antioncogenes p16 and p53 might play a promotive role in the tumorigenesis and development of bladder tumors.

Adult↗

PubMed2000

[Inhibition of monocytes adhesion to the intima of arterial wall by local expression of antisense monocyte chemotactic protein-1].

OBJECTIVE: To study the mechanism of monocyte recruitment in atherogenesis and to clarify the effect of monocyte chemotactic protein-1 (MCP-1) in this process. METHODS: Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue. RESULTS: Expression of antisense MCP-1 mediated by recombinant LNCX plasmid/lipsomal complex gene transfer enabled to inhibit MCP-1 gene expression and adhesion of monocyte to the intima. CONCLUSION: MCP-1 plays an important role on the recruitment of monocytes in the arterial wall, which provides a potential clue in developing a gene therapy project for the prevention and treatment of atherogenesis.

Animals↗

PubMed2000

[The conscious of mutual infiltration of nature of things (Cmnn) and alchemy and swallowing stone].

By Cmnn, it refers to an idea that a matter can possess the nature of another matter completely irrelevant to the former one. This idea consistently played an important role in the pursuing of selection of longevity medicines in ancient time. Through this idea, the alchemists connected the life activity of the human body to the natural materials like gold and cinnabar to reinforce their belief of longevity.

Alchemy↗

PubMed2000

[The effects of hypertonic saline solution perfusion on the isolated hearts of burned rats].

OBJECTIVE: To observe the effect of hypertonic saline solution perfusion on the function of isolated hearts of burned rats. METHODS: The isolated rat heart was taken as the model. The hearts from normal control and burned rats were perfused by improved isotonic (Na(+) 118 mmol/L) and hypertonic Krebs-Henseleit (Na(+) 215 mmol/L) solutions, respectively. The dynamic changes of the cardiac function were monitored during heart perfusion. RESULTS: The cardiac contractive and diastolic function were transiently inhibited and followed by quick recovery during the perfusion with hypertonic solution in both control and burned rat hearts. But when the hypertonic solution was replaced by isotonic, there appeared significant enhancement of the cardiac contractive and diastolic function. These findings indicated that hypertonic solution exerted more powerful influence on the contractive and diastolic function of burned rat heart. CONCLUSION: Hypertonic saline solution could influence the cardiac contractile and diastolic function significantly. And the magnitude of the effects was correlated with the basic cardiac condition.

Animals↗

PubMed2000

[Antiphospholipid thrombosis syndrome and acquired activated protein C resistance].

OBJECTIVE: To explore the correlation between antiphospholipid antibodies (APA) activated protein C resistance (APCR) and antiphospholipid thrombosis (APL-T) syndrome and investigate the mechanism of thrombosis in APL-T syndrome. METHODS: ELISA, APTT-LA and APTT+/-APC methods were used to detect anticardiolipin antibodies (ACA), lupus anticoagulants (LA) and APCR in 20 APL-T syndrome patients respectively. RESULTS: Twenty patients were diagnosed as APL-T. The patients were classified in three ways. According to etiology, 14 of 100 systemic lupus erythematosus (SLE) patients were diagnosed as "secondary" APL-T, and 6 of 16 "unexplained" thrombosis and spontaneous abortion as "Primary" APL-T syndrome. According to antibodies, 12 of 20 patients with positive LA were diagnosed as lupus anticoagulant thrombosis syndrome (LA-T), 12 of 20 patients with positive ACA as anticardiolipin antibodies thrombosis syndrome (ACA-T). According to clinical presentation: 7 of 20 patients with deep vein thrombosis were classified as type I APL-T, 3 of 20 patients with cerebrovascular thrombosis as type II, 1 of 20 patients with vein cerebrovascular thrombosis and spontaneous abortion as type IV (mixing), and 9 of 20 patients with spontaneous abortion as type V (fetal wastage type). The ACAs and LA are strongly associated with APL-T. There is close relationship between APCR and APL-T. CONCLUSION: The acquired APCR might be an important reason for thrombosis in APL-T caused by APA and LA.

Activated Protein C Resistance↗

PubMed2000

[Study of platelet membrane glycoproteins and its fibrinogen binding reaction in patients with cerebral infarction and diabetes mellitus].

OBJECTIVE: To investigate the change of platelet membrane glycoproteins and its fibrinogen binding reaction in patients with cerebral infarction and type II diabetes mellitus (DM), and assess their value in the study of thrombosis and pro-thrombosis state. METHODS: By flow cytometry, the platelet glycoprotein (GP) I b, GP II b, GP III a, GP II b- III a complex and P-selectin expression as well as fibrinogen binding reaction were analyzed in 85 patients with type II DM and 28 with cerebral infarction, and compared with that in 30 normal subjects. RESULTS: The platelet membrane GP II b- III a complex, P-selectin expression and fibrinogen binding reaction were higher, while the platelet surface GP I b was lower in the patients with cerebral infarction and type II DM with vascular disease than those in normal group. The GP II b and GP III a were not significantly changed. There was no difference of these parameters between the type II DM without vascular disease patients and normal group. CONCLUSION: The platelet fibrinogen binding reaction, P-selectin and GP II b- III a complex expression were increased while the GP I b decreased in patients with cerebral infarction and type II DM with vascular disease.

Adult↗

PubMed2000

[The impact of hepatitis B virus infected patients or donors on the outcome of hematopoietic stem cell transplantation].

OBJECTIVE: To investigate the impact of recipients and/or donors infected with hepatitis B on the outcome of hematopoietic stem cells transplantation (HSCT). METHODS: We analyzed retrospectively the transplantation outcome in 26 of 164 hematological diseases patients who and whose donors were infected with hepatitis prior to transplantation. RESULTS: (1) Three of the 26 patients developed VOD after HSCT, the incidence (11.5%) is significant higher than that in patients and donors who did not infected with HBV (P < 0.05). (2) Two of 5 patients transfused hematopoietic stem cells from HBsAg + donors developed hepatitis B. (3) Four patients with allogeneic stem cell transplantation developed hepatic failure (HF) which occurred during cyclosporin (CsA) being tapered off or withdrawal. The incidence of HF in patients and/or donors infected with HBV (15.4%) is obviously higher than that in patients and donors who did not (0.08%, P < 0.01). (4) In 4 patients with HbeAg +, 2 patients died of HF after allogeneic HSCT, the other 2 patients survived after autologous HSCT. CONCLUSION: Donors and recipients infected with hepatitis B are not contraindication of HSCT, but patients with allogeneic HSCT are in danger to develop LF during CsA being tapered off or withdrawal.

Adolescent↗

PubMed2000

[Retroviral-mediated high efficient in vitro expression of human coagulation factor VIII].

OBJECTIVE: To develop a retroviral-mediated high efficient expression system of human coagulation factor VIII. METHODS: The retroviral vector LNC-VIIIBD was generated by cloning a B-domain-deleted FVIII cDNA (760aa - 1639aa) into retroviral vector pLNCX. Several cell lines including NIH3T3, CHO, COS-7 and human hepatic cell line L-02 were infected with viral supernatant from the highest productive PA317 clones. The antigen and procoagulant activity of human FVIII in the cell culture medium were measured by ELISA assay and one-stage method, respectively. RT-PCR was performed for the detection of F VIII BD mRNA. RESULTS: Human FVIII was expressed in all four target cells. The highest expression was observed in NIH3T3, the procoagulant activity of secreted FVIII was up to 1.6 U, and the FVIII antigen was 500 ng by 10(6) cells/ml in 24 hours, respectively. CONCLUSION: The constructed retroviral vector was able to generate high level expression of human FVIII in some cell lines, and it might have potential utility in the gene therapy for Hemophilia A.

3T3 Cells↗