Search PubMed⌕ Search

Biomedical subjects

Z Wang

Publications and source records attributed to Z Wang.

At least 829 records · Page 46Linked to original sources
PubMed2000

[A report of two Chinese familial Budd-Chiari syndrome].

OBJECTIVE: To investigate the etiology of two Chinese familial Budd-Chiari syndrome (BCS). METHOD: Four patients with familial BCS (from A and B families), and the other 41 family members were detected by angiography, ultrasound Dopler, etiology analysis and Factor V Leiden (FvL) mutation analysis. RESULT: Four BCS patients were proved by angiography, 2 by ultra sound Dopler in family A. Ten members in family A were varicosis in low extremeties. FvL mutation was detected in 4 of 6 patients and 2 normal family members. A II(2), A III(7, 11, 15,) B II(10) and B III(5) had FvL mutation. The FvL mutations were compatible with Mendel hereditary law. CONCLUSION: FvL mutation may be one of main risk factors and varicosis in low extremeties may be another risk factors for familial BCS.

Adolescent↗
PubMed2000

[Construction of membrane-anchored interleukin-6 receptor beta subunit mutant and its effect on IL-6 signal transduction].

OBJECTIVE: To investigate whether the membrane-anchored gp130 mutant containing the second and the third fibronectin type III modules of the extracellular region could still transduce IL-6 signal. METHOD: The membrane-anchored gp130 mutant cDNA was amplified by the overlap extension PCR and inserted into the mammalian expression vector pRc/RSV. The mutant was proved to be effectively expressed in SKO-007 cells and Jurkat cells by dot blot hybridization. The mutant's effects on IL-6 signaling in these two kinds of cells were detected by electrophoresis mobility shift assay (EMSA). RESULTS: In SKO-007 cells, the mutant could enhance the activated APRF, but the effect was not as strong as that of wide-type gp130. IL-6 could not activate nuclear factors in Jurkat cells. After the expression vectors pRc/RSVgp130 and pRc/RSV were transfected into Jurkat cells, IL-6 could activate APRF, but could not do NF-IL6. Similar to the results in SKO-007 cells, the effects of the mutant was also weaker than that of wild-type gp130. CONCLUSION: Although the mutant could still transduce IL-6 signal, it is not as efficiently as the wild-type gp130. It suggested that the Ig-like domain of gp130 also played an important role in IL-6 signal transduction.

Antigens, CD↗
PubMed2000

[Clinical and experimental study on treatment of bronchial asthma with pingchuan oral liquid].

OBJECTIVE: To observe the clinical effect of Pingchuan oral liquid, including Hanchuan oral liquid (HCOL) and Rechuan oral liquid (RCOL), and to explore its mechanism. METHODS: HCOL and RCOL were prepared based on the therapeutic principles of eliminate phlegm, remove blood stasis, lower reversed Qi flow and relieve asthma. HCOL was used to treat 50 patients of bronchial asthma in Group A and RCOL for 55 patients in Group B, and they were compared with 52 patients treated with Guilong Kechuanning capsule in the control group. The relevant animal experiment was also conducted. RESULTS: The total effective rate of HCOL was 88.0%, that of RCOL was 90.9%, they were significantly higher than that of the control (73.1%, P < 0.05). Moreover, the two new preparations showed the effects in easing main symptoms and signs of asthma, decreasing peripheral eosinophilic granulocyte count and immunoglobulin level, and improving pulmonary function superior to those of Guilong Kechuanning capsule (P < 0.05, P < 0.01). Experimental study showed that they could lower blood thromboxane B2 and endothelin, raise blood nitric oxide and 6-keto-prostaglandin F1 alpha. CONCLUSION: Pingchuan oral liquid is obviously effective in treating bronchial asthma, the mechanism possibly lays on adjusting immune function, anti-allergy and antagonizing inflammatory media.

6-Ketoprostaglandin F1 alpha↗
PubMed2000

Experimental study on apoptosis of HL-60 cell induced by arsenic trioxide.

OBJECTIVE: To explore the mechanism of arsenic (arsenic trioxide, AS2O3) in treating leukemia. METHODS: The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied ith flow cytometry. Function on telomerase activity was also observed. RESULTS: After the effect of arsenic on HL-60 cell line, PI negative/AnnexinV-FITC positive cells increased, it mainly acts at the G2-M phase that suggests the apoptosis takes place, mild inhibition on telomerase was also found 48 hours later. CONCLUSION: Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells.

Apoptosis↗
PubMed2000

[Effect of chrysotile on nitric oxide production and anti-oxidasic activity in rabbit alveolar macrophages].

This study aimed to explore the role of nitric oxide (NO) and nitric oxide synthase (NOS) in chrysotile asbestos-caused diseases. The production of NO2-/NO3- (the final product of NO) and the alterations of NOS, SOD and GSH-Px activity were investigated when rabbit alveolar macrophages (AM) were stimulated by UICC chrysotile. The results revealed that with the dosage elevation of UICC chrysotile, the rabbit alveolar macrophages showed: (1) increased mortality, decreased survival activity; (2) increased product of NO2-/NO3-, decreased SOD and GSH-Px activity; (3) increased NOS activity in the lower dosage groups, but decreased NOS activity in the higher dosage groups. There was significantly negative correlation between NO release and SOD (or GSH-Px) activity (r1 = -0.7125, P < 0.05; r2 = -0.8496, P < 0.05 respectively). Moreover, the significantly positive correlation between NO release and NOS activity was found in the lower dosage groups, but significantly negative correlation in the higher dosage groups. These findings suggest that chrysotile could induce the alveolar macrophages to increase NO release and to decrease SOD and GSH-Px activity, which may play a role in the process of asbestos-caused diseases.

Animals↗
PubMed2000

[Experiment study on fit of anterior osseointegrated implant frameworks related to the number of fixture abutments].

Good fitness of osseointegrated frameworks was a key factor to protect implant for function. The purpose of this study was to evaluate the fitness of anterior implant fixed bridge in one-piece casting made of Ni-Cr alloy in different numbers of fixture abutments. The specimens were measured by the three-dimensional measurement system (German Opton uMM550). The results demonstrated that there were statistically significant differences in dimensional changes among one, two or three fixture numbers, the space of retention was 50-150 microns, and the flow of retention was 100-250 microns.

Dental Abutments↗
PubMed2000

[Influence of the administrators of family planning on the spread of norplant in countryside of Sichuan].

The purpose of the study was to understand the family planning administrators' knowledge of Norplant and their fluence on the spread of Norplant in countryside of Sichuan. 156 administratoras were surveyed. It was found that most of the administrators worked hard on education, propagation, organization and management of using Norplant, and they recognized the contraceptive efficacy of Norplant. However, the educational level of the administrators was generally low. They were short of the training on Norplant related knowledge(training rate was only 36.5%). The degree of the users' satisfaction and the administrators' knowledge on Norplant varied between areas. The result indicated that the basic quality of the administrators and their knowledge of Norplant were influences on the spread of Norplant in the countryside of Sichuan. Soit is very important to improve the basic quality and knowledge of the administrators of family planning.

Adolescent↗
PubMed2000

[The correlation between anticardiolipin antibodies and disease activity in patients with systemic lupus erythematosus].

This study was conducted to determine the prevalence and clinical association of anticardiolipin antibodies (ACA) in serum of patients with systemic lupus erythematosus (SLE). ACA (IgG, IgA, IgM) was measured by enzyme-linked immunosorbent assay (ELISA) in the serum sample of 87 patients of SLE. The prevalence rates of ACAIgG, ACAIgA and ACAIgM were 62.1%, 52.9% and 35.6% respectively. The titers of ACAIgG and ACAIgM were positively correlated with the disease activity (r = 0.2763, P = 0.044; r = 0.3294, P = 0.018); ACAIgG, ACAIgA and ACAIgM were negatively correlated with complement C3 (r = -0.4737, P = 0.000; r = -0.2990, P = 0.017; r = -0.2758, P = 0.029); and ACAIgG was negatively correlated with complement C4 (r = -0.4079, P = 0.005). The titers of ACAIgA in patients with thrombosis was higher than that in patients without thrombosis. The patients with central nervous system involvement (CNS-SLE) had significantly higher ACAIgG and ACAIgM titers, compared with those without CNS-SLE. The results indicate there is a correlation between ACA titers and disease activity. In patients with thrombosis, the titer of ACAIgA is higher, and in patients with CNS-SLE, the titers of ACAIgG and ACAIgM are higher.

Adolescent↗
PubMed2000

[Effects of bacterial protective liquid on radiation biology effect of mouse bone marrow cells].

To study the relationship between bacterial protective liquid (BPL) and the biological effect of mouse bone marrow cells by gamma-ray irradiation, apoptosis and the number of CD4+, CD8+ and NK cells were analyzed by flow cytometry(FCM), the results showed that BPL decreased the apoptosis and increased the proliferation of cells(P < 0.01). The theorem of action was related with raising the percentage vitality of NK cells and the quantity of CD4+ and CD8+. This study proved that BPL might have protective effect against radiation injury.

Animals↗
PubMed2000

[Effect of pretreatment on the measurement of folate in foods].

In order to measure the content of folate in foods accurately, to hydrolyze and extract folate in foods with suitable pretreatment is necessary because folate in foods is always in polyglutamate structure. The pretreatment of samples including hydrolysis with phosphate buffer(0.05 mol/L, pH 6.8) once or twice, with chick pancreatin, protease and/or amylase, and a combining of trienzyme methods was compared and discussed. A microbiological method with lactobacillus casei was used. A high result of folate in foods could be obtained when extracting folate enriched samples or vegetables with phosphate buffer once or twice respectively, or hydrolyzing "extracted oral liquids" with chick pancreatin, or when extracting samples rich in protein and/or starch with trienzyme. The RSD of interassay and intrassay were 6.5%-9.9% and 3.5%-14.1% respectively, and the recovery rate was 86.8%-106.8%.

Animals↗
PubMed2000

[Animal experimental study on endocrine factors affecting alveolar repair].

OBJECTIVE: To investigate the endocrine effects on activities of alkaline phosphatase (ALP) and acid phosphatase (ACP) and to obtain the mechanism of the resorption and regeneration of the alveolar bone. METHODS: 24 dog models with two-side osseous infrabony pocket were divided into 4 groups randomly and were studied by histologic examination, enzyme cytochemistry and image analysis system. RESULTS: The activities of ALP and ACP, except ACP in the sixth month significantly decreased in the group of thyroidism comparing with that of the control group (P < 0.01), but only the activities of ALP significantly decreased in groups of ovariectomy and diabetes (P < 0.05, P < 0.01), and there was close relationship between enzyme activities and histopathologic variations. CONCLUSION: Alveolar bone repair was regulated by systemic endocrine factors and was associated with the activities of ALP and ACP osteoblasts of and osteoclasts.

Acid Phosphatase↗
PubMed2000

[The devitalization of tumor suppressor genes in oral squamous cell carcinoma].

OBJECTIVE: To study the expression of anti-oncogenes nm23, P16, P53 of squamous cell carcinoma within one sample. METHODS: A total of 27 cases of oral squamous cell carcinoma came from our the First Clinical College, China Medical University from 1997 to 1998, and none of them had the history of radioactivity therapy or chemical therapy. In them, 8 cases were in the early period, and 19 cases were in the late period. 15 cases were male and 12 cases were female. The samples were fixed in formaldehyde solution, embedded with paraffin, sliced in 5 microns, and analysed by ABC immunohistochemistry staining. The first antibodies were anti-P16, anti-nm23, anti-P53 respectively. The criteria of judgment were the following: For nm23, the case that cytoplasm or nucleus were buffy particles or ball was positive. For P16, the case that nucleus were brown was positive, while the case with the light brown cytoplasm and unstained nucleus was negative. The stained nucleus of interstitial cells were treated as the control. For p53, the positive protein product was assembled in the carcinoma cell nucleus, so the nucleus with buffy or brown particles was positive. According to the criteria, 5 high power sights were selected from each case, more than one hundred cells were counted. The case that the positive cells was more than 30 percentage was high expression, and less than 30 percentage was low expression. Two doctors completed the procedure respectively, and only the same results could be qualified. RESULTS: In the 27 cases, the devitalization of nm23 and p16 was showed in low expression, p53 in high expression. The devitalization rate: p16 was 66.7%, nm23 was 48.1%, and p53 was 25.9%. In 14 cases, combined mutation of two inhibitory genes were seen. 9 cases were p16 and nm23, among them, 4 cases had transferred to cervical lymph nodes (In all the cases, there were 6 cases who had cervical lymph node transference). CONCLUSION: p16 genetic changes are more often detected in oral squamous cell carcinoma than the other genetic changes; In a large portion of cases, there were combined genetic changes instead of single gene mutation.

Adult↗
PubMed2000

[Ultrastructure of the lymph capillaries in human dental pulp].

OBJECTIVE: The purpose of this article is to observe the ultrastructures of the lymph capillaries in the human dental pulps. METHODS: Investigations have been performed on dental pulps from 8 human permanent teeth, including 4 mandibular first premolars and 4 maxillary first premolars, which were extracted for orthodontic reason. As soon as possible, we cleaved the teeth longitudinally and immersed in the fixative solution at 4 degrees C for 1 hour, then kindly removed the pulps and immersed in the same fixative solution for 3 hours, then routinely postfixed, dehydrated, embedded, and prepared the ultrathin sections (50-70 nm) without semithin section localization. The lymph vessels were distinguished from blood vessels on ultrathin sections by their morphological features. RESULTS: We have observed three fundamental kinds of intercellular junctions, overlapping(51%), end to end(36.1%), and interdigistating (10.6%). A few complex junctions also could be seen, but we failed to find the open junction. The Weibel-Palade bodies were occasionally present in the lymphatic vessels endothelial cells of young dental pulps. And we even found the lipofuscins in the cytoplasm of the endothelium. Some inclusion bodies, such as paracrystalline inclusions and myeloid bodies, also could be seen. There were many micropinocytotic vesicles in the cytoplasm of the lymphatic endothelium, the diameter of the most vesicles was about 50-80 nm, some was about 100-500 nm. Some vesicles appeared to lie free within the cytoplasm, and some were seen to touch or open onto the luminal or abluminal surfaces of the endothelia, which suggested that many vesicles were not simple discrete units but rather from parts of chains that reached either luminal or abluminal surfaces. It was reported that micropinocytotic vesicles, open junctions and intraparietal channels provided fluid transport. CONCLUSION: Dental pulp is a special organ, and the lymph capillaries in it have their own ultrastructural features.

Adolescent↗
PubMed2000

[Expressions of five protein kinase C isoforms in salivary adenoid cystic carcinoma].

OBJECTIVE: The protein kinase C (PKC) family of isoforms plays the key role in the regulation of cellular events, including cell proliferation, differentiation and canceration. Previous work from our laboratory has shown the alteration of PKC and its inhibitor activities in salivary adenoid cystic carcinoma (SACC) [Sun Changfu, et al. West China Journal of Stomatology, 1998, 16(3): 201-203]. This study aimed to further explore the relationship between PKC isoforms and canceration of SACC. METHODS: Expressions of five isoforms of PKC (alpha, beta I, beta II, epsilon and delta) were examined in cytosolic and membranous fraction of specimen homogenates from SACCs and were compared with the adjacent normal tissues by immunoblotting. Equal amounts of cytosolic and membrane protein from control and experimental groups were simultaneously subjected to sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membranes, and reacted with first and second antibodies; Color development and image analysis were performed. RESULTS: 1. Cytosolic PKC-alpha, PKC-beta I, and PKC-beta II were expressed in SACCs and adjacent normal tissues. The Expressions of these three cytosolic isoforms were reduced significantly in SACCs compared with the adjacent normal tissues; 2. Membranous PKC-alpha was only detected in SACCs and the membranous translocation of cytosolic PKC-alpha was also seen. 3. The expressions of PKC-epsilon and PKC-delta were not detected in both SACCs and adjacent normal tissues. CONCLUSION: There was close relationship between changes of expression of PKC isoforms (alpha, beta I, beta II) and carcinogenesis of SACC. We postulated that there may be relationship between the membranous translocation of PKC-alpha and hematogenous lung metastasis in SACC.

Carcinoma, Adenoid Cystic↗
PubMed2000

[The detection of Epstein-Barr virus in middle ear effusion of secretory otitis media].

OBJECTIVE: To study the involvement of Epstein-Barr Virus (EBV) in the pathogenesis of secretory otitis media (SOM). METHOD: Epstein-Barr Virus (EBV) in blood serum, oral gargle and middle ear effusion was detected by polymerase chain reaction (PCR) method in 34 patients with secretory otitis media and 20 normal subjects. RESULT: The detection rate of EBV in serum and oral gargle in the SOM group was remarkably higher than that in normal control group (P < 0.01). EBV positive rate of middle ear effusion was higher than that of serum group in SOM individuals. CONCLUSION: EBV plays a role in the pathogenesis of secretory otitis media (SOM).

Adolescent↗
PubMed2000

[Investigation on the changing regularity of the relationship between retention index and temperature of the GDX series stationary phases].

A systematical measure of the polarity scale of the Chinese porous polymer beads of this series of stationary phase has been carried out, including the McReynolds constant at 120 degrees C by using squalane as non-polar stationary phase and the relative constant to GDX-103 at 180 degrees C. Based on the above work, two kinds stationary phases GDX-101 and GDX-103 were selected, on which the changing regularity of the relationship between retention index and temperature of the five "probes" of stationary polarity determination, including benzene, butanol, 1-nitropropane, 2-pentanone and pyridine were investigated. At the same time, the changing regularity with temperature of the relative McReynolds constant delta I of GDX-101 to GDX-103 was studied. It is found that Ip-theta c curve and delta I-theta c curve all showed a turning point at the temperature of 180 degrees C and that there was a maximum value of delta I at the temperature of 180 degrees C. So we think that the retention mechanism of this kind of stationary phase changed at 180 degrees C.

1-Butanol↗
PubMed2000

[The construction of a recombined E. coli strain with stable and high production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)].

Plasmid pJMC2 was constructed by cloning the parDE fragment of RK2 into pTZ18U-PHB which harbored phaCAB from Alcaligenes eutrophus and was transferred into E. coli HMS174 and E. coli JM107 separately. It is very stable in its hosts cultured in medium without ampicillin. E. coli HMS 174(pTZ18U-PHB) and E. coli JM107(pTZ18U-PHB) produced P(3HB-co-3HB) in a low phosphate concentration medium(18 mmol/L). The proportion of 3-hydroxyvalerate(3HV) in the polymer was 5%-8%. A fed-batch culture of E. coli HMS174(pJMC2) was conduct in a 5 L automatically controlled fermentor, the final dry cell weight, P(3HB-co-3HV) content, and th 3HV proportion were 42.5 g/L, 70% and 4.9% respectively.

Culture Media↗