Biomedical subjects

Yu-Feng Huang

Publications and source records attributed to Yu-Feng Huang.

At least 37 records · Page 2Linked to original sources

PubMed2005

[Advances in researches on the relationship between single nucleotide polymorphism and prostate cancer].

Single nucleotide polymorphism (SNP) has been regarded as the third generation of heredity markers with many marked characteristics. It has been developed many new experimental techniques for detecting SNP in recent years, such as TaqMan probe technique, gene chip technique, denaturing high performance liquid chromatograph, matrix assisted laser desorption ionization-time off light mass spectrometry and minisequencing technique. Great progress has been made in researches on human tumors with SNP as heredity markers. This article reviews the genes and SNP related to the development of prostate cancer, including prostate specific antigen response component, enzymes, hormones and their receptors, cell cycle regulating protein, cytokines, adhesion molecules, vitamins and so on, and aims to explore the possible mechanism of the disease.

Humans↗

PubMed2005

[Comparison of clinical efficacy between Huorongbushen and indomethacin in patients with oligospermia or asthenospermia].

OBJECTIVE: To compare the clinical efficacy and safety between Huorongbushen and indomethacin in patients with oligospermia or asthenospermia. METHODS: 86 patients with oligoasthenospermia were received at our clinic of andrology. They were randomly divided into group A and B. The patients in group A received Huorongbushen 48 g daily for 3 months, and the other patients in group B were given indomethacin 50 mg daily for 3 months. The sperm parameters of the patients were analyzed by computer-assisted sperm analysis system before and after treatment. RESULTS: Patients of group A were significantly improved in sperm concentration, forward sperm motility, total sperm motility, straight line velocity and average path velocity. Patients of group B remained unimproved in sperm concentration and were significantly improved in other sperm parameters. The patients in group A reported a significantly higher sperm concentration than that in group B. There was no significant difference between group A and B in other sperm parameters. CONCLUSION: Compared with indomethacin, Huorongbushen is efficacious for oligoasthenospermia with lower side effects.

Adult↗

PubMed2005

Re-activation characteristics of preserved aerobic granular sludge.

In some industrial plants, wastewater was intermittently or seasonally generated. There may be periods during which wastewater treatment facilities have to be set into an idle phase over several weeks. When wastewater was generated again, the activated sludge flocs may have disintegrated. In this experiment, re-activation characteristics of aerobic granular sludge starved for 2 months were investigated. Specific oxygen utilization rate (SOUR) was used as an indicator to evaluate the metabolic activity of the sludge. The results revealed that aerobic granular sludge could be stored up to two months without running the risk of losing the integrity of the granules and metabolic potentials. The apparent color of aerobic granules stored at room temperature gradually turned from brownish-yellowish to gray brown. They appeared brownish-yellowish again 2 weeks after re-activation. The velocity and strength of granules after 2-month idle period could be fully restored about 3 weeks after re-activation. Metabolic activity, however, dropped to 15.8 mg O2/(g MLVSS x h), i.e. 74 % reduction after 2 months of storage. After restarting the reactor, it took 2 weeks that SOUR of up to 48.5 mg O2/(g MLVSS x h) was achieved. A stable effluent COD concentration of less than 150 mg/L was achieved during the re-activation process.

Aerobiosis↗

PubMed2005

[Oral sildenafil demonstrates the effectiveness and well tolerance in the treatment of patients with erectile dysfunction].

Erectile dysfunction (ED) is a common disease in male populations, and the morbidity is increasing. Currently, sildenafil is the first line oral therapy in the treatment of erectile dysfunction, and widely used by the patients from different areas, who are variable in ages, etiologies and degrees of severity, and by the patients with different diseases such as cardiovascular problems, diabetes mellitus and nephropathy. The drug manifests the effectiveness and well tolerance. In this paper, we review and summarize some relevant articles published to share information with the medical colleagues.

Administration, Oral↗

PubMed2004

Relationship between lipocalin-type prostaglandin D synthase and alpha-glucosidase in azoospermia seminal plasma.

BACKGROUND: [corrected] To determine the correlation of lipocalin-type prostaglandin D synthase (L-PGDS) and alpha-glucosidase in semen. METHODS: We analyzed 68 seminal plasmas for lipocalin-type prostaglandin D synthase (L-PGDS) and alpha-glucosidase, L-PGDS was analyzed by ELISA. The semen donors were categorized in 3 groups: normal, obstructive and non-obstructive azoospermia. We then evaluated their correlation. RESULTS: The difference of L-PGDS concentration (P<0.001) and alpha-glucosidase activity (P<0.001) among the 3 clinical groups was statistically significant. Correlation between L-PGDS concentration and alpha-glucosidase was also statistically significant. L-PGDS concentration correlated positively with alpha-glucosidase activity (r=0.882). CONCLUSIONS: L-PGDS in seminal plasma, like alpha-glucosidase, suggests an obstruction of the seminal ducts and may be a potential marker that may aid in the differential diagnosis of obstructive and non-obstructive azoospermia.

Adult↗

PubMed2004

Col1a1 promoter-targeted expression of p20 CCAAT enhancer-binding protein beta (C/EBPbeta), a truncated C/EBPbeta isoform, causes osteopenia in transgenic mice.

CCAAT enhancer-binding protein (C/EBP) transcription factors regulate adipocyte differentiation, and recent evidence suggests that osteoblasts and adipocytes share a common pluripotent progenitor in bone marrow. However, little is known about the role of C/EBP transcription factors in the control of osteoblast differentiation or function. In this study, the function of C/EBP transcription factors was disrupted in osteoblast lineage cells by overexpressing a naturally occurring dominant negative C/EBP isoform. Expression of FLp20C/EBPbeta was driven by a 3.6-kb Col1a1 promoter/first intron construct, and four transgenic (TG) mouse lines were established. Northern blotting and reverse transcription-PCR indicated that the transgene was targeted to bone, with lower levels of expression in lung, skin, and adipose tissue. TG mice from two lines showed reduced body weight compared with wild type littermates. All TG lines showed evidence of osteopenia, ranging from mild to severe, as evidenced by reduced trabecular bone volume. Severely affected lines also showed reduced cortical bone width. Dynamic histomorphometry demonstrated an associated decrease in mineral apposition and bone formation rates. Long bones and calvariae of TG mice showed reduced COL1A1 and osteocalcin mRNA levels and increased bone sialoprotein mRNA, consistent with an inhibition of terminal osteoblast differentiation. Ex vivo analysis of primary osteoblast differentiation showed similar effects on marker expression and reduced expression of the mature osteoblast marker Col2.3-green fluorescent protein, demonstrating a cell-autonomous effect of the transgene. These data suggested that C/EBP transcription factors may be important determinants of osteoblast function and bone mass.

Adipocytes↗

PubMed2004

Occurrence of Porphyromonas gingivalis and Tannerella forsythensis in periodontally diseased and healthy subjects.

BACKGROUND: The purpose of this study was to compare the prevalence and level of Porphyromonas gingivalis (P. gingivalis) and Tannerella forsythensis (T. forsythensis) in subgingival plaque samples from both healthy individuals and periodontal patients in different age groups. METHODS: A total of 498 subgingival plaque samples were studied. These samples were collected from 407 individuals diagnosed with periodontal disease (210 adult periodontitis [AP], 78 rapidly progressive periodontitis [RPP], and 119 refractory periodontitis [Ref-P] cases) and 91 healthy (H) subjects. P. gingivalis and T. forsythensis were detected by indirect immunofluorescent assay using species-specific polyclonal antisera to P. gingivalis strain (FDC 381) and T. forsythensis strain (FDC 335). The prevalence of P. gingivalis and T. forsythensis was compared by chi-square analysis. Differences in P. gingivalis and T. forsythensis levels among various periodontal status and age groups was determined by one-way analysis of variance and Fisher's multiple comparison tests. The association between the presence of P. gingivalis or T. forsythensis in different periodontal status and age groups was measured using odds ratios. RESULTS: P. gingivalis was found in 85.7% (P < 0.0001) and T. forsythensis in 60.7% (P = 0.0002) of diseased subjects compared to 23.1% and 39.6%, respectively, in healthy subjects. P. gingivalis, but not T. forsythensis, was detected more frequently in any diseased group than in the H group in every age group (P<0.0001). No significant difference was found in the prevalence of P. gingivalis and T. forsythensis among age groups, except T. forsythensis was more prevalent in the age group of 40 to 59 years than in the age group < 20 years (chi2 = 3.93, P = 0.047) in the H group. The mean level of P. gingivalis and T. forsythensis was significantly higher in diseased groups than in the H group (P < 0.0001). The odds ratio for P. gingivalis in the AP group (25.0) was greater than any other group for P. gingivalis or T. forsythensis compared to the H group. CONCLUSIONS: These data suggested that P. gingivalis is closely associated with the pathogenesis of periodontitis and that it may not be a normal inhabitant of periodontally healthy subjects. T. forsythensis is also important in the pathogenesis of periodontal disease; however, whether it causes periodontal disease or is a secondary invader of periodontal lesions remains to be determined.

Adult↗

PubMed2004

[A de novo complex chromosomal rearrangement including translocation on 1,5 and 12 in a female carrier with her healthy girl].

We reported in the paper one case of a de novo complex chromosomal rearrangement (CCR) involving three different chromosomes,1, 5 and 12. Two pregnancies of the female carrier over three years resulted in two spontaneous abortions. Initial cytogenetic analysis of her peripheral lymphocyte by G banding suspected a karyotype 46,XX,t(1;5;12)(1pter -->1q25::12q24-->12qter;5qter--> 5p11::1q25-->1qter;12pter -->12q24::5p11-->5pter). Fluorescense in -situ hybridization (FISH) was used to confirm the karyotype 46,XX,t(1;5;12)(1pter-->1q23::12q22-->12qter;5qter-->5p11::1q25-->1qter;12pter-->12q22::1q23-->1q25::5p11-->5pter). Seven years later she was pregnant again and refused to have prenatal diagnosis. The fetus is normal both in phenotype and karyotype. Comparing previously reported female CCR carriers with the case, we conclude that female CCR carriers may not always present spontaneous abortion or have offspring with congenital malformation and can have chance to get a healthy child.

Abortion, Habitual↗

PubMed2003

[Molecular cloning of human sperm surface protein P34H gene and semi-quantitative analysis of its expression in testis and epididymidis].

OBJECTIVES: To identify, clone and sequence human sperm protein P34H gene and perform semi-quantitative analysis of its expression in testis and epididymidis. METHODS: Total RNA was prepared from human epididymidis tissue. cDNA fragment encoding human P34H was amplified by RT-PCR using specific primers, and then was cloned into pGEM-T vector. Inserted P34H gene was sequenced by ABI 377 DNA Sequencer. Meanwhile, semi-quantitative analysis of P34H expression in testis and caput, corpus, cauda epididymidis was done by RT-PCR based on beta-actin as an inner control. RESULTS: Human sperm protein P34H gene was successfully cloned and its cDNA sequence was submitted to GenBank(Accession No. AF515625). CONCLUSIONS: The cloning of P34H gene may be useful for the further basic and clinical studies on P34H.

Base Sequence↗

PubMed2003

[The distribution and detection of androgen receptor in extra-testis tissues].

Androgen receptor(AR) plays an important role in modulating the effects of androgen on target cells. It is well known that AR is mainly existed in testis. This paper reviewed the distribution of AR and its mRNA in prostate, epididymis, skin of penis, and some other tissues in non-genital system and tumors, such as the skin of scalp, hippocampus, fat, gastric cancer, cancer of larynx, and so on. Besides, this paper also reviewed the detection methods to AR, and further investigated the function of androgen.

Androgens↗

PubMed2003

[Andrology in the 21st century: keeping pace with the time].

This editorial briefly reviewed the history of andrology, its rapid development in recent years, the conception of male health, international andrology studies, the achievements of Chinese andrologists and the existing problems. It is highly necessary for Chinese andrology community to strengthen the international academic communication, join the International Society of Andrology and attend its activities actively.

Andrology↗

PubMed2003

[Detection and clinical significance of phospholipase A2 in semen of male infertile patients].

OBJECTIVES: To explore the clinical application of anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb) for male infertility. METHODS: Enzyme-linked immunoabsorbent assay (ELISA), immunocytochemistry(ICC), as well as flow cytometry (FCM) analysis were established using two strains anti-human seminal plasma PLA2 McAb prepared by our laboratory to detect the PLA2 content in human seminal plasma and the anterior head region of spermatozoa, respectively. Then the PLA2 content in male infertile patients were compared with that in normal control with fertility. The seminal routine analysis was performed by computer-assisted semen analysis (CASA). RESULTS: The PLA2 content of infertile groups were (31.13 +/- 14.49) ng/ml in azoospermic patients, (17.71 +/- 12.45) ng/ml in oligospermic patients and (16.46 +/- 11.31) ng/ml in patients with normal sperm density, which were all higher than that of normal controls [(8.09 +/- 3.15) ng/ml, P < 0.01]; There was significantly negative correlation between PLA2 content in seminal plasma and sperm density(r = -0.602, P < 0.05), while there was insignificant correlation between PLA2 and sperm motility or percentage of motility. The PLA2 content in the anterior head region of spermatozoon of male infertile groups was significantly lower than that of normal controls by ICC and FCM(P < 0.01). CONCLUSIONS: PLA2 in human seminal plasma is closely related to male fertility, and the PLA2 deficiency in the head of spermatozoa may be one of the reasons causing male infertility. The methods detecting PLA2 content in seminal plasma and the head of spermatozoa can provide powerful evidences for exploring the mechanism of male infertility.

Adult↗

PubMed2003

[Cloning and eukaryotic expression of human lipocalin-type prostaglandin D synthase in Pichia Pastoris].

OBJECTIVES: To express human testis Lipocalin-type prostaglandin D synthase in Pichia Pastoris for further research on biological function and clinical applications. METHODS: Human testis L-PGDS gene coding region was amplified from plasmid pGEX-2T/htL-PGDS by PCR with a deletion of the signal peptide sequence. The DNA fragment was inserted into pPIC9 to construct yeast expression plasmid followed by transformation of the yeast GS115 strain with electroporation. The recombinant his-tag protein was induced to express by methanol. RESULTS: The sequence of the amplified DNA fragment was identical to that of human testis L-PGDS previously reported. The recombinant protein was found with a molecular mass of 27,000 on SDS-PAGE, which was identical to that of native L-PGDS. CONCLUSIONS: Secretory expression of human L-PGDS was obtained in Pichia Pastoris.

Cloning, Molecular↗

PubMed2003

[Significance of neutrophil elastase in diagnosis of male genital tract infection].

Neutrophil Elastase(NE) is a kind of neutral proteinase. Its value in the diagnosis of male genital tract infection has attracted increasing attention in recent years. NE has special diagnostic value in screening the typical genital tract infection caused by Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum and Trichomonas, as well as other kinds of genital infection, such as leukocytospermia and periorchitis. NE has been considered as a specific marker of the male genital tract infection.

Chlamydia trachomatis↗

PubMed2003

[Detection of cholesterol ester transfer protein in semen of infertile patients].

OBJECTIVES: To detect the cholesterol ester transfer protein (CETP) levels in semen of infertile patients and evaluate the correlation between CETP in semial plasma and infertility. METHODS: One hundred and sixty-three infertile patients and fifteen fertile males were selected randomly. The routine examination of ejaculates was fulfilled by computer aided semen analysis (CASA). The CETP levels in all seminal plasma samples and fifty-five serum samples were detected by ELISA method. RESULTS: The CETP levels in infertile patients and fertile males were (2.21 +/- 1.23) microgram/L and (1.40 +/- 0.45) microgram/L, respectively. There were no significant differences between the two groups(P > 0.05). And there were no significant differences of CETP levels in seminal plasma among groups of azoospermia(n = 29), oligoasthenozoospermia (n = 58), oligospermia(n = 15), asthenozoospermia(n = 44) and normozoospermia(n = 17) in the infertile patients(P > 0.05). The CETP in seminal plasma and serum were detected in 55 infertile patients, and there was no correlation between CETP levels in seminal plasma and serum using Spearman analysis(r = 0.009, P > 0.05). The mean CETP level in seminal plasma was almost 1/1,000 of that in serum. CONCLUSIONS: The CETP level in seminal plasma is extremely low and has no relation with the changes of sperm density or motility. It may ensure the integrity of sperm membrane before the sperm enters into female genital tract.

Adult↗

PubMed2003

[A tentative research of testosterone supplement therapy on male senile dementia].

OBJECTIVES: To understand the hormone levels in male senile dementia patients and the effects of testosterone supplement theraphy (TST) on male senile dementia. METHODS: The general health condition, mental health condition, hormone levels, and some other aspects were evaluated in 9 patients with male senile dementia without significant contradiction for TST. 9 patients were treated with oral testosterone undecanoate 120 mg per day for six weeks, and relative changes were observed, detected and recorded. RESULTS: In 9 cases of male senile dementia, the average score was 5.33 in mini-mental state examination (MMSE), 14.56 in Hamilton depression scale (HAMD), 5.0 in geriatric depression scale (GDS), 32.56 in partial androgen deficiency of the aging male (PADAM) measuring form. The volume of testis was small (8.33 ml on average). After 1 week of having finished TST, the levels of serum testosterone (T) dropped significantly (dropped from average 2.88 ng/ml to 2.47 ng/ml, P < 0.05) in 9 patients with TST. General evaluation with clinician's global impression(CGI) for 9 patients showed 2 cases fail and 7 cases with progress (3 of them progress significantly). Average score dropped in HAMD form (10.67) and PADAM form (28.11). Physical strength and psychological status improved significantly, but vasomotor symptoms and sexual function failed response to the treatment. CONCLUSIONS: The levels of androgen is low, and the symptoms of androgen deficiency are readily seen in male senile dementia. TST can improve life quality of most patients to some extent.

Aged↗

PubMed2003

[Detection of sexual hormone in semen of patients with idiopathic azoospermia or oligospermia and its significance].

OBJECTIVES: To detect the sexual hormone level in semen of patients with idiopathic azoospermia and oligospermia, and further analyze the relationship between sexual hormone and idiopathic azoospermia and oligospermia. METHODS: 50 male patients with idiopathic azoospermia, 50 in idiopathic oligospermia and 50 male controls with normal sperm density were selected. The sperm density and sexual hormone in semen were detected respectively by routine semen analysis and chemical luminescence technique. RESULTS: The values of LH were (5.19 +/- 0.67) IU/L and (4.77 +/- 0.68) IU/L, and those of FSH were (1.90 +/- 0.79) IU/L and (2.27 +/- 0.25) IU/L in idiopathic azoospermia and oligospermia respectively, and the values of LH and FSH were (2.19 +/- 0.22) IU/L and (1.61 +/- 0.14) IU/L in normal control group respectively. There were significant differences in the values of LH and FSH between idiopathic azoospermia and normal control group(P < 0.01 or P < 0.05). The values of PRL were (6.25 +/- 0.51) ng/ml and (6.33 +/- 0.34) ng/ml, and those of T were (1.51 +/- 0.12) ng/ml and (1.68 +/- 0.71) ng/ml in idiopathic azoospermia and oligospermia respectively, and the values of PRL and T were (6.36 +/- 0.32) ng/ml and (1.83 +/- 0.09) ng/ml in normal control group respectively. There were no significant difference in the values of PRL between idiopathic azoospermia, oligospermia and normal control group, but there were significant differences of T between idiopathic azoospermia and normal control. Compared with 0.84 +/- 0.20 in normal control, the values of T/LH were 0.35 +/- 0.09 and 0.29 +/- 0.04 in idiopathic oligospermia and azoospermia respectively and there were significant differences(P < 0.05). CONCLUSIONS: The changes of LH, FSH and T values may be one of the reasons that cause the dysfunction of spermatogenesis and sperm maturation in patients with idiopathic azoospermia and oligospermia. The study of semen hormone may lead to new strategies in the treatment to azoospermia and oligospermia.

Adult↗

PubMed2003

[Advances in sperm membrane antigens].

A series of sperm membrane antigens, the important molecular markers associated with the program of spermatogenesis and maturation of sperm, which also are for attachment of the physiological mechanism of the sperm-egg interaction and fusion and the pathological changes in the infertility, were reviewed. Some traditional crucial sperm membrane proteins, such as FA-1, PH-20, fertilin, have been cloned and sequenced, and some novel gene which encode new proteins associated with the sperm membrane also have been screened out successively. All of these provided a basement for lucubration of these proteins and the research of the contraception vaccine.

Animals↗