Biomedical subjects

Yu-Feng Huang

Publications and source records attributed to Yu-Feng Huang.

At least 55 records · Page 3Linked to original sources

PubMed2003

[Infection of Chlamydia trachomatis and apoptosis of spermatogenic cells].

OBJECTIVE: To evaluate the relationship between infection of Chlamydia Trachomatis(Ct) and apoptosis of spermatogenic cells. METHODS: Apoptotic spermatogenic cells were examined by Wright-Giemsa staining and the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate(dUTP)-biotin nick-end labeling(TUNEL) technique. RESULTS: Apoptosis rate of Ct infective group was significantly higher than that of normal group(P < 0.01). CONCLUSIONS: Ct infection may cause the apoptosis of spermatogenic cells, which affords an objective evidence for illustrating the mechanism of Ct-infection-induced male infertility.

Adult↗

PubMed2003

[Inhibin B levels of serum and seminal plasma in fertile and infertile males].

OBJECTIVES: To investigate the possible differences in the inhibin B levels of seminal plasma and serum between fertile and infertile males and to obtain information on the relation between serum inhibin B or seminal plasma inhibin B and spermatogenesis. METHODS: Semen and blood samples were collected from fertile(n = 20), oligospermia(n = 20), asthenospermia(n = 22) and non-obstructive azoospermia(NOA) (n = 20) males at 8:00 am = 10:00 am. Semen parameters were analyzed. Levels of inhibin B in seminal plasma and serum, ACP, Fru, alpha-Glu in seminal plasma, serum levels of FSH, T, LH were determined. RESULTS: Both levels of serum inhibin B and levels of seminal plasma inhibin B correlated significantly negatively with serum FSH(r = -0.536, P < 0.001 vs r = -0.288, P = 0.01), and statistically positively with sperm concentration(r = 0.49, P < 0.001 vs r = 0.48, P < 0.001). There was positive correlation between levels of seminal plasma inhibin B and activity of alpha-Glu in seminal plasma (r = 0.377, P = 0.001). The difference in levels of seminal plasma inhibin B was found only between fertile males or asthenospermia and NOA (P < 0.01 and P < 0.05, respectively). However, significant differences in levels of serum inhibin B were found not only between males with normal sperm concentration (including fertile males and asthenospermia) and NOA (P < 0.01), fertile males and oligospermia (P < 0.05), but also between oligospermia and NOA (P < 0.05). There was no correlation between serum inhibin B and seminal plasma inhibin B. CONCLUSIONS: Both levels of serum inhibin B and seminal plasma inhibin B could reflect testis spermatogenesis status. Levels of seminal plasma inhibin B could also reflect the function of seminiferous duct, but the wide range of values limited its applicability.

Adult↗

PubMed2003

[Genetic variations and possible therapeutic ways of male infertility].

Chromosome aberrant and some gene mutations play an important role in male infertility. A knowledge of a variety of new genetic causes is crucial for the prevention and treatment of male infertility. This review focused on known genetic causes of male infertility, male fertility-related candidate genes from knockout mice, genetic analysis and treatment of infertility in men.

Animals↗

PubMed2003

[Advances in the studies of Chlamydia trachomatis infection in males].

Chlamydia trachomatis(Ct) infection in reproductive tract is one of the most common sexually transmitted diseases at present. However, relatively fewer studies are made on Ct infection in men. The paper reviews the epidemiology of Ct infection in males, Ct infection and male diseases, Ct infection and male infertility, and the detection of Ct infection in men. It aims at providing a theoretical basis and a practical guide for the prevention and control of Ct infection in men.

Chlamydia Infections↗

PubMed2003

[Advances in microinsemination with spermatid].

Men with non-obstructive azoospermia(NOA) can now be treated by using intra-oocyte round spermatid injection(ROSI) or elongated spermatid injection(ELSI). Spermatids can be retrieved from semen or from testis biopsy specimens. But the rates of fertilization and pregnancy with spermatids have been disappointing. Many problems limiting success rate and hindering a wide application of this technique still remain unresolved, including the incomplete maturation of spermatid nuclear, oocyte activation and identification of a live spermatid.

Azoospermia↗

PubMed2003

[Chinese traditional medicine yi kang ling to treat immunity infertility caused by anti-sperm antibody: the experimental research. I].

OBJECTIVE: To observe the effects of Chinese traditional medicine Yi Kang Ling (YKL) on immunity infertility caused by anti-sperm antibodies (AsAb). METHODS: Based on the animal model of immunity infertility, seventy-five pairs of New Zealand rabbits were divided into three groups: YKL treatment group (sub-divided into mini-, midi- and maxi-dosage groups), prednisone treatment group and non-treatment group. Five pairs of normal rabbits were used as control. The AsAb from the rabbit serum were detected on the 15th, 30th and 45th day of treatment respectively. The sperm density, activity, the mobility and AsAb of seminal plasma from the obedient rabbits were determined. RESULTS: Statistics showed that on the 45th day in mini- and maxi-YKL and prednisone treatment groups the positive serum AsAb reversing ratio reached 100%, and the seminal plasma AsAb reversing ratios were 85% in mini- and maxi-YKL group, 83% in midi-YKL and prednisone groups, while in non-treatment group the reversing ratios of the positive serum AsAb and seminal plasma AsAb were only 20% and 25% respectively. There were also remarkable differences (P < 0.05) in both serum AsAb and seminal plasma AsAb on the 45th day of treatment between YKL, prednisone treatment groups and the non-treatment group. CONCLUSIONS: YKL can effectively reverse the AsAb positive results, hence increasing sperm mobility and improving sperm quality.

Animals↗

PubMed2003

[Role of sulfogalactosylglycerolipid and sulfolipidimmobilizing protein 1 in sperm-egg plasma membrane interaction].

Sperm-egg plasma membrane interaction is one of the important steps of mammalian fertilization. Many sperm and egg surface proteins are reported to be involved in sperm-zona pellucida interaction. Sulfogalactosylglycerolipid(SGG) is the major sulfoglycolipid in the germ cells of mammalian and lower vertebrates, mainly in the sperm head. It is a differentiation marker in spermatogenesis restricted to the zygotene and early pachytene spermatocytes. Sulfolipidimmobilizing protein 1 (SLIP1) is the major sulfoglycolipid of mammalian germ cells and eggs, with the same localization as SGG in the sperm. SLIP1 binds specificity to SGG, both playing a vital role in sperm-egg interaction. This article is aimed at reviewing the localization of SGG and SLIP1 in the germ cell surface and their role and related mechanism in gamete formation.

Animals↗

PubMed2002

Autosomal aberrations associated with testicular dysgenesis or spermatogenic arrest in Chinese patients.

AIM: To analyze the relationship between autosomal aberrations and testicular dysgenesis or spermatogenic arrest in Chinese patients and to map the corresponding regions on each autosome in regard to the recorded aberrations accompanying these distubances. METHODS: One hundred and nineteen cases of aberrant karyotypes with testicular dysgenesis, azoospermia or oligozoospermia reported in five Chinese journals and one monograph were analyzed. For each autosome, the type and frequency of chromosomal aberrations were counted and the regions corresponding to the disturbances were mapped out. RESULTS: Chromosomes 13, 14, 9, 21 exhibited a high frequency of aberration and bands 14q11 and 13p11 were the two regions showing the highest linkage to testicular dysgenesis or infertility. The frequency of chromosomal aberrations was higher in bands 9p11 and 22q than in others. CONCLUSION: Autosomes 13, 14, 9 and 21 in the order of importance play a critical role in testicular development and spermatogenesis and other autosomes may also contribute; the following regions, 14q11, 13p11,9p11, and 22q, are of high significance.

Asian People↗

PubMed2002

[Construction and identification for cell strain of anti-human seminal plasma phospholipase A2 monoclonal antibody].

OBJECTIVES: To establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb). METHODS: After having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2 was regarded as an antigen to immune BALB/C mouse to produce anti-human seminal plasma PLA2 McAb. The PLA2 McAb sensitivity and specificity were performed by ELISA technique and Western-blot analysis, respectively. RESULTS: The molecular weight of PLA2 depurated with 245 fold purification from human seminal plasma was about 34,900, while the sensitivity and typing of its McAb were 1:5(6)-1:5(8) and IgM (kappa) with a satisfied Western-Blot results. CONCLUSIONS: The PLA2, which had not been reported in international and domestic papers, may be a new type of PLA2. The establish of its McAb will provide significant tools for the research of the relationship between PLA2 in human seminal plasma and male fertility.

Antibodies, Monoclonal↗

PubMed2002

[Detection of human sperm morphology and acrosome reaction with Coomassie brilliant blue staining].

OBJECTIVES: To evaluate the application of Coomassie brilliant blue (CBB) G250 staining for the detection of human sperm deformity rate, rate of intact acrosome and acrosome reaction. METHODS: The smear of spermatozoa before and after capacitation and induced acrosome reaction with progesterone (P) were stained with 0.05% CBB G250 and Wright-Giemisa solution respectively, and visualized with light microscopy. The deformity rate of spermatozoa, rate of intact acrosome and acrosome reaction were calculated. RESULTS: There was no any difference in detection of deformity rate of spermatozoa and rate of intact acrosome with CBB G250 and Wright-Giemisa staining(P < 0.05). The sperm population of acrosome reaction with induced P was divided by CBB staining into two types: positive staining with dark violet blue on acrosome cap and pale or negative staining on the same area. The rate of the latter was increasing with increasing inductive time, maybe representative of the rate of acrosome reaction. The mean rate was(75.1 +/- 3.8)% after induced for 1 h. CONCLUSIONS: CBB G250 staining is a reliable method for assessment of the human sperm morphology and acrosome reaction.

Acrosome↗

PubMed2002

[Ultrastructural observation of normal spermatozoa incubated with ROS in vitro].

OBJECTIVES: To observe ultrastructural changes of spermatozoa after incubate with reactive oxygen species (ROS). METHODS: Spermatozoa of normal physiological functions selected from semen samples by Percoll gradient centrifugation technique were regarded as normal sperm models in present study. Ultrastructural changes of spermatozoa observed by transmission electron microscope after model spermatozoa were incubated with ROS generated by hypoxanthine and xanthine oxidase under aerobic environment. RESULTS: After model spermatozoa were incubated with ROS, impairment of various extent in membrane and acrosome of spermatozoa and abnormality in mitochondria of spermatozoa were found. CONCLUSIONS: Excessive ROS may cause ultrastructural change in membrane, acrosome and mitochondria of spermatozoa and impair function of spermatozoa.

Adult↗

PubMed2002

[Preliminary study on hens immunized with recombinant human testis prostaglandin D synthase DNA].

OBJECTIVES: In order to identify whether the hens immunized with recombinant human testis prostaglandin D synthase (rhtL-PGDS) DNA can produce anti-L-PGDS antibody. METHODS: The serum were got from the hens immunized with recombinant plasmid pGEX-2T/htL-PGDS DNA (100 micrograms) every 2 weeks for 2 times. The exist of anti-L-PGDS antibody and its titer were tested with agarose dual immunodiffusion and ELISA with rhtL-PGDS as antigen. RESULTS: The serum anti-L-PGDS antibody in hen immunized with pGEX-2T/htL-PGDS DNA were confirmed and its titer tested by ELISA was 1:2,048. CONCLUSIONS: It is feasible to produce anti-L-PGDS antibody by immunizing hens with recombinant pGEX-2T/htL-PGES DNA.

Animals↗

PubMed2002

[Isolation and identification of spermatids from mouse testis].

OBJECTIVES: To develop a simple and effective method by which spermatids can be isolated from mouse testis. METHODS: Combination of enzymatic digestion was used to prepare suspension of spermatogenic cells from adult mouse testis, and then a modified discontinuous Percoll gradient (15%, 22%, 30%, 40%, 50%, 60%) centrifugation method was introduced to isolate spermatids from the cellular suspension. The content of spermatids in each isolated fraction by Percoll method was determined by morphology (Wright-Giemsa staining) and flow cytometry analysis, and the viability of spermatogenic cells was assessed using Eosin Y exclusion test. RESULTS: More than 97% of the testicular cells remained their viability after enzymatic digestion. After Percoll centrifuged, six fractions were formed. In each isolated fraction, the 22% fraction contained mostly spermatids(mean 86.7%) and cell viability was more than 85.5%. While in the 30% fraction, immature spermatogenic cells were present, and more than 92% of the cells remained their viability. CONCLUSIONS: A large of relatively purified spermatids can be isolated from mouse testis by enzymatic digestion combined discontinuous Percoll gradient centrifugation method.

Animals↗

PubMed2002

[The distribution of androgen receptor and its coexistence with gonadotropin releasing hormone receptor in rat submaxillary].

OBJECTIVES: To study the coexistence of androgen receptor(AR) and GnRH receptor(GnRHR), and further identify that submaxillary is a target organ of androgen and GnRH in SD Rat. METHODS: Sequential deparaffinized sections of SD rat submaxillary were immunostained with SABC method. The first antibodies were rabbit anti-rat GnRH idiotypic antibodies and mouse anti-rat androgen receptor antibodies. RESULTS: AR immunoreactive cells were found in glandular epithelial cells of serous acinus and epithelial cells in all gland ducts. While the distribution of GnRHR coincides with that of AR. The immunoreactive substances were distributed in cytoplasm of all positive cells with negative nuclei. CONCLUSIONS: The results showed that AR existed in submaxillary and was widely distributed in glandular epithelial cells with distributive pattern similar to those of GnRHR. It suggests that submaxillary is a target organ of androgen, responsible for modulating biological function of submaxillary.

Animals↗

PubMed2002

[A serum marker of spermatogenesis--inhibin B].

Inhibin B is a glycoprotein secreted by testis, consisting of two disulfide-linked subunits, an alpha-subunit and a beta B-subunit. Serum inhibin B levels are significantly negatively correlated with the serum FSH levels in males, exerting a negative feedback on FSH secretion. In males the circulating levels of inhibin B increase shortly after birth and peak at 4-12 months of age, then decrease to low levels from 3-9 year. From the onset of puberty, the levels of inhibin B gradually increase. By pubertal stage II, the adult levels of inhibin B have been reached. At stage III of puberty, a negative correlation between inhibin B and FSH levels is present and persists from stage III of puberty onward. At 20-30 year of age, the levels of inhibin B reach another peak, then gradually decline with increasing age. The men with hypospermatogenesis and spermatogenesis arrest have significantly lower levels of inhibin B than those with normal spermatogenesis. The men with Sertoli-cell-only syndrome (SCO) have extremely low levels of inhibin B. There is a closely correlation between the presence of SCO and the level of serum inhibin B. A significantly positive correlation is also observed between testis volume and inhibin B level, as well as between sperm count and inhibin B level. The inhibin B is a direct product of the seminiferous tubules, reflecting the total testicular tissue. The measurable inhibin B production in adult requires the presence of germ cells. Inhibin B is regarded as a serum marker of spermatogenesis. The determination of serum inhibin B in males can be used to assess the spermatogenesis of infertile men, to diagnose the cryptorchidism and precocious puberty, to predict the outcome of testicular sperm extraction in men with non-obstructive azoospermia, and to evaluate the damage to spermatogenesis in men after radiotherapy or chemiotherapy.

Biomarkers↗

PubMed2002

[Epididymal sperm protein P34H and male reproduction].

During epididymal transit, mammalian spermatozoa acquire new surface proteins that are necessary for gamete interaction. P34H, a member of the short-chain dehydrogenase/reductase(SDR) superfamily, is acquired on the acrosomal cap of human spermatozoon during its maturation arising within epididymis. P34H has been shown to be involved in sperm-zona pellucida interaction. Research revealed that the occurrence of low concentration of sperm protein P34H were significant amongst the idiopathic infertile male population and P34H protein could also be considered as a marker of epididymal sperm maturation in human. Therefore the level of sperm protein P34H is proposed to be a auxiliary diagnostic tool for male infertility. This paper reviews the molecular properties and regulation of the expression of P34H and its association with male reproduction.

Acyl-CoA Dehydrogenase↗

PubMed2002

[Advances in eukaryotic expression systems].

The increasing popularity of eukaryotic expression systems can be attributed to their capability of performing many post-translational modifications. At present, There mainly are three expression systems including yeast expression system, insect cell expression system and mammalian cell expression system. The methylotropic yeast Pichia Pastoris usually utilizes alcohol oxidase promoter to drive the expression of foreign gene. Recently, a continuous fermentation has been developed in Pichia Pastoris with the glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. The baculovirus-mediated insect cell expression system is considered to be safe, powerful, but cell-lytic. Baculovirus-S2 system uses the popular and genetically well understood Drosphila S2 cells which do not appear to be lysed after infection. In mammalian cell expression system, recombinant adenovirus are attracting a great deal of attention as a highly efficient gene transfer vehicle. The frequency of Ad vector rescue by homologous recombination in E. coli and Cre-mediated site-specific recombination is significantly higher than by homologous recombination in vivo. Tetracycline-regulatable system is a widely used mammalian cell inducible expression system due to its high efficiency and stringency.

Adenoviridae↗

PubMed2002

[Advances in sperm capacitation].

Sperm must be capacitated before sperm-ovum fusion. Capacitation was once considered as hyperactivation. But now many investigators thought that capacitation wasn't equal to hyperactivation, and that sperm hyperactivation might be a moiety of capacitation or the result of capacitation. In the present, the methods used to study sperm capacitation include fertilization in vitro, induction of sperm acrosome reaction, FITC-labeled chlortetracycline and plant hemoagglutinin. The studies on sperm capacitation in vitro mainly focused on the inductive substances of sperm capacitation and subsequent results analysis. It could lay foundation for the manifestation of molecular mechanism of sperm capacitation and destination of sperm capacitation in molecular levels.

Adenylyl Cyclases↗