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Biomedical subjects

Y Yasui

Publications and source records attributed to Y Yasui.

At least 199 records · Page 11Linked to original sources
PubMed1986

Mystacial vibrissae representation within the trigeminal sensory nuclei of the cat.

Somatotopic arrangements of axon terminals of primary afferent fibers innervating follicles of the mystacial vibrissae were examined in the cat by the transganglionic horseradish peroxidase (HRP) method. Forty to 60 hours after injecting HRP into a single or a group of vibrissal follicles, transported HRP was visualized by the tetramethylbenzidine technique. HRP-labeled axon terminals were distributed in the ventral subnucleus of the principal sensory trigeminal nucleus (ventral Vp), in the oral and interpolar spinal trigeminal nuclei (Vo and Vi), and in the caudal spinal trigeminal nucleus (Vc) (layer I, deep part of layer II, layers III-V) with its spinal extension into the dorsal horn of the first cervical cord segment (rostral C1). In cross sections through the caudal parts of the ventral Vp, Vi, and layer IV of the Vc and rostral C1, a single mystacial vibrissa was represented in a one-to-one fashion by a patch of dense terminal arbors of primary afferent fibers. The more dorsally a horizontal row of the mystacial vibrissae was located, the more ventrally was it represented in the ventral Vp, the more ventrolaterally in the Vi, and the more ventrally in layer IV of the Vc and the rostral C1. In addition, the more anteriorly a vibrissa was located in a horizontal row of the mystacial vibrissae, the more medially was it represented in the ventral Vp, the more ventromedially in the Vi, and the more laterally in layer IV of the Vc and rostral C1; the most posteriorly located vibrissae in the horizontal rows of the mystacial vibrissae were represented along the lateral border of the ventral Vp and Vi, and most medially in layer IV of the Vc and rostral C1. Thus, the representation pattern in the ventral Vp was rotated clockwise at about 45 degrees angle in the Vi, and projected as a mirror image in layer IV of the Vc and rostral C1. It was also indicated that the anterior-posterior arrangement of the mystacial vibrissae was represented in a rostral-caudal organization within layer IV of the Vc and rostral C1. It was also indicated that the anterior-posterior arrangement of the mystacial vibrissae was represented in a rostral-caudal organization within layer IV of the Vc and rostral C1. Patchy patterns probably replicating the distribution of the vibrissae on the face of the cat were also revealed by the cytochrome oxidase histochemical staining in cross sections through the caudal parts of the ventral Vp, Vi, and layer IV of the Vc and rostral C1.

Animals↗
PubMed1986

A morphological evidence of direct connections from the cochlear nuclei to tensor tympani motoneurons in the cat: a possible afferent limb of the acoustic middle ear reflex pathways.

The central circuitry of the acoustic middle ear reflex activating the tensor tympani muscle was studied in the cat by tracer methods using horseradish peroxidase (HRP), wheat germ agglutinin (WGA) or HRP conjugated with WGA (WGA-HRP). The results indicate that the dorsal and ventral cochlear nuclei send fibers to motoneurons innervating the tensor tympani muscle, bilaterally with an ipsilateral dominance.

Afferent Pathways↗
PubMed1986

Trigeminal nerve branches containing primary afferent fibers to the dorsal division of the principal sensory trigeminal nucleus: a transganglionic horseradish peroxidase study in the cat.

Trigeminal nerve branches containing primary afferent fibers to the dorsal division of the principal sensory trigeminal nucleus (Vp) were examined in the cat by the transganglionic horseradish peroxidase method. The results indicate that the pterygopalatine, superior alveolar, infraorbital, buccal, lingual, inferior alveolar and mental nerves contain primary afferent fibers terminating in the dorsal division of the Vp probably conveying sensory information chiefly from the intraoral structures.

Afferent Pathways↗
PubMed1986

Direct projections from the parvocellular part of the posteromedial ventral nucleus of the thalamus to the infralimbic cortex in the cat.

It was shown in the cat by the anterograde and retrograde WGA-HRP method that the medial portion of the parvocellular part of the posteromedial ventral nucleus of the thalamus sent fibers ipsilaterally to the caudoventral part of the infralimbic cortex on the medial surface of the frontal lobe, as well as the orbital cortical regions and the rostrodorsal part of the lateral amygdaloid nucleus.

Amygdala↗
PubMed1986

[The effect of immunochemotherapy with PSK in malignant tumors of the female reproductive organs].

In our series of investigations concerning immunopotentiators, we evaluated the host immunocompetence of patients triggered by PSK. Our subjects for screening consisted of seven patients with malignant tumors of female reproductive organs chosen at random from our patients. Four of them were treated with chemotherapy (CAPF) alone and served as controls, whereas the other three were administered PSK, 3.0 g/day, plus chemotherapy as above. Cellular and humoral immunities were measured at three points. Firstly prior to chemotherapy, secondly, two weeks after the start of chemotherapy, and thirdly, prior to next chemotherapy. The results indicated higher values of T-cell subset ratio OKT-4/8 of 2.73 +/- 0.56 on the average for cellular immunocompetence as compared to 1.35 +/- 0.45 for normal subjects in the PSK-administered group, whereas there was no increase in the non-PSK-administered group, in which the value was 1.28 +/- 0.30. Thus, PSK was considered to extert an immunopotentiating effect on the T-lymphocytes.

Adjuvants, Immunologic↗
PubMed1986

[Comparative studies of antitumor activities of oxalate (1R, 2R-cyclohexanediammine) platinum (II) and cis-dichlorodiammine-platinum (II) using ovarian cancer transplanted into nude mice].

A new platinum coordination complex, l-OHP [oxalato (1R, 2R-cyclohexanediammine) platinum (II)], has been shown to be an active anticancer agent in animals. In the present study comparisons of the effects on human ovarian carcinomas transplanted into nude mice (OCl-1-N, OS-4-N, OY-1-N) between l-OHP and CDDP, were investigated. l-OHP (6 mg/kg, 9 mg/kg) and CDDP (3 mg/kg, 6 mg/kg) were administered intraperitoneally in a schedule of every four days for three doses. Simultaneously the tumor size and the body weight were measured and the peripheral WBC and serum BUN were examined. The results were as follows: 1) The antitumor activity of l-OHP corresponded to that of CDDP in proportion to the Pt content and mouse LD50. 2) In l-OHP, there was no elevation of the BUN value and less decrease in body weight, but the peripheral leukopenia was more severe, although reversible. l-OHP is an active anticancer agent against xenografts in nude mice and is strongly recommended for clinical application.

Animals↗
PubMed1985

Coexistence of neuropeptides in projection neurons of the thalamus in the cat.

Coexistence of neuropeptides was suggested by double-staining immunohistochemistry in projection neurons in the thalamus of the cat; cholecystokinin (CCK)-like immunoreactivity (LI) and vasoactive intestinal polypeptide (VIP)-LI in the rostral group of the intralaminar nuclei, CCK-LI and neurotensin (NT)-LI in the anterodorsal nucleus and NT-LI and VIP-LI in the laterodorsal nucleus.

Animals↗
PubMed1985

Cerebral cortical projections to the reticular regions around the trigeminal motor nucleus in the cat.

Cerebral cortical regions which send projection fibers to the reticular regions around the trigeminal motor nucleus were identified in the cat by the horseradish peroxidase (HRP) method. The reticular region around the trigeminal motor nucleus are known to contain many interneurons for masticatory motoneurons. After injections of HRP into the reticular regions around the trigeminal motor nucleus, HRP-labeled neuronal cell bodies in the cerebral cortex were found in layer V. They were distributed bilaterally in the orbitofrontal cortical regions, mainly in the rostral extension of the orbital gyrus close to the presylvian sulcus; more were located in the floor and lateral bank of the presylvian sulcus than in the crown of the orbital gyrus. After injections of HRP conjugated with wheat germ agglutinin (WGA-HRP) into these cortical regions, many labeled presumed axon terminals were distributed bilaterally in the reticular regions around the trigeminal motor nucleus; mainly in the region ventral to the trigeminal motor nucleus and in the intertrigeminal region between the main sensory trigeminal nucleus and the trigeminal motor nucleus. Terminal labeling in these regions was more prominent after WGA-HRP injection into the lateral bank of the presylvian sulcus than after WGA-HRP injection into the crown of the orbital gyrus. Thus, the present results indicate that the main part of the cortical region projecting directly to the reticular regions around the trigeminal motor nucleus in the cat is folded into the presylvian sulcus.

Animals↗
PubMed1985

Leucine-enkephalin-like immunoreactive afferent fibers to pudendal motoneurons in the cat.

Leucine-enkephalin-like immunoreactive (ENK-LI) afferent fibers to the feline homologue of the Onuf's nucleus (pudendal motoneurons) originate mainly from lamina X of the sacral cord. They make synaptic contacts chiefly upon dendrites of pudendal motoneurons; the synaptic terminals most often contain pleomorphic synaptic vesicles and occasionally round synaptic vesicles. Large-cored vesicles in the axon terminals within the Onuf's nucleus often showed ENK-LI.

Animals↗
PubMed1985

Multipolar neurons and axodendritic synapses in the mesencephalic trigeminal nucleus of the cat.

In the mesencephalic trigeminal nucleus (MTN) of the adult cat, multipolar neurons with 1-9 smooth dendritic processes were labeled with horseradish peroxidase which was applied to central cut ends of the masseter, deep temporal, medial pterygoid, superior alveolar and inferior alveolar nerves. These constituted 40% of the total population of MTN neurons; most of them were jaw-closing muscle afferent neurons and located mainly at the levels of the superior colliculus. Axon terminals were found electron microscopically to make synaptic contacts upon horseradish peroxidase-labeled dendritic profiles of MTN neurons.

Animals↗
PubMed1985

Direct cortical projections to the parabrachial nucleus in the cat.

Direct projections from the cerebral cortex to the parabrachial nucleus in the cat were examined by the horseradish peroxidase (HRP)method. When HRP was injected into the parabrachial nucleus, retrogradely labeled neuronal cell bodies were seen, bilaterally with an ipsilateral predominance, mainly in the orbital gyrus, the lateral bank of the presylvian sulcus, and a restricted region in the infralimbic cortex on the medial surface of the frontal lobe (stereotaxic coordinates; Fr: 22, L: 1, H: -1); all labeled neurons were in deep pyramidal cell layer. After injecting HRP conjugated to wheat germ agglutinin (WGA-HRP) into the cortical regions where retrogradely labeled neurons were found after injecting HRP into the parabrachial nucleus, anterogradely labeled cortical fibers were traced to the parabrachial nucleus. Corticoparabrachial fibers originating from the orbital gyrus and the lateral bank of the presylvian sulcus ran ipsilaterally through the internal capsule and the cerebral peduncle down to the lower brainstem, whereas those from the infralimbic cortex coursed down ipsilaterally through the medial forebrain bundle. These cortical fibers to the parabrachial nucleus were distributed bilaterally with an ipsilateral predominance. Cortical fiber terminals in the parabrachial nucleus were topographically arranged: Corticoparabrachial fibers from the lateral bank of the presylvian sulcus ended most massively in the dorsal part of the lateral parabrachial nucleus. Corticoparabrachial fibers from the orbital gyrus ended most heavily in the medial parabrachial nucleus and less heavily in the lateral parabrachial nucleus. Corticoparabrachial fibers from the infralimbic cortex ended mostly in the parabrachial regions surrounding the brachium conjunctivum.

Animals↗
PubMed1985

Topographical projections from the posterior thalamic regions to the striatum in the cat, with reference to possible tecto-thalamo-striatal connections.

Projections from the posterior thalamic regions to the striatum were studied in the cat by the anterograde tracing method after injecting wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) into the caudalmost regions of the lateroposterior thalamic nucleus (caudal LP), suprageniculate nucleus (Sg) and magnocellular division of the medial geniculate nucleus (MGm). The results were further confirmed by the retrograde tracing method after injecting WGA-HRP into the regions of the caudate nucleus (Cd) and putamen (Put) where afferent fibers from the caudal LP, Sg and MGm were distributed. Fibers from the MGm, Sg or caudal LP were distributed mainly in the medial, middle or lateral part of the caudal half of the putamen (caudal Put), respectively. Although there was a considerable overlap, thalamostriatal fibers from the caudal LP terminated more caudally than those from the MGm. On the other hand, thalamocaudate fibers from the MGm, Sg and lateral part of the caudal LP overlapped with each other in the ventrolateral part of the caudal half of the caudate nucleus (caudal Cd). Fibers from the medial part of the caudal LP were distributed in the ventral part of the caudal Cd. In the superior colliculus (SC) of the cats with WGA-HRP injections in the caudal LP, retrogradely labeled neuronal cell bodies were mainly seen ipsilaterally in the superficial SC layer, and simultaneously, anterogradely labeled axon terminals were observed in the striatum. On the other hand, when WGA-HRP was injected into the Sg or MGm, labeled SC neurons were mainly located in the intermediate and deep SC layers. Thus, ascending impulses from the superficial SC layer may possibly be conveyed ipsilaterally via the caudal LP to the ventral and ventrolateral parts of the caudal Cd and the lateral part of the caudal Put, whereas those from the intermediate and deep SC layers may be relayed via the Sg and/or MGm to the ventrolateral part of the caudal Cd and the middle and medial parts of the caudal Put.

Animals↗
PubMed1985

[Combination chemotherapy with peplomycin, cis-platinum and mitomycin C in gynecologic cancer].

Eight patients with gynecologic cancer (cervix: 6, corpus: 1 and vulva: 1) were treated with combination chemotherapy, PPM therapy consisting of continuously infused PEP 4mg/m2 days 1-5, CDDP 13 mg/m2 days 1-5, and MMC 3mg/m2 day 1. This was repeated every three to five weeks. Six of the eight patients were evaluable, two had a complete response and one had a partial response, for an overall response rate of 50%. Because of hematological toxicity, blood transfusion was carried out in four patients. Nephrotoxicity and pulmonary toxicity were slight. Nausea and vomiting were controlled with dexamethasone and domperidone. PPM therapy is considered to be an effective and useful combination chemotherapy for patients with gynecologic cancer.

Adenocarcinoma↗