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Biomedical subjects

Y Yasui

Publications and source records attributed to Y Yasui.

At least 181 records · Page 10Linked to original sources
PubMed1988

[Favorable response of advanced hepatocellular carcinoma to proper hepatic arterial administration of cytokines and the significance of the administration].

The objectives in administering anti-cancer therapeutics to the feeding artery of the tumor are to allow the agent to come in direct contact with the tumor cells, to lower the concentration of the agent in body circulation, to lessen the severity of side effects, and to augment efficacy of the agent. A remarkable partial regression was observed in two patients with advanced hepatocellular carcinoma, both at the stage in which surgical excision was diagnosed impossible; one was given successive and daily bolus administration of OH-1, a anti-tumor agent consisting of natural human tumor necrosis factor-alpha (nHuTNF-alpha) and natural human interferon (nHulFN-alpha), and the other a successive and daily combined bolus administration of OH-1 and 5-FU. On investigating the role of the anti-cancer activity of OH-1 by analyzing the NK activity of rat liver large granular lymphocytes, we found that the NK activity was suppressed dose-dependently by nHuTNF-alpha, but not significantly. Thus, an increase in TNF dose in hepatic artery therapy seems undesirable from the standpoint of NK activity. The authors are presently carrying out investigations to elucidate the effector mechanism of the anticancer activity of OH-1.

Animals↗
PubMed1988

[Pharmacokinetic and therapeutic studies of the administration of peplomycin by continuous subcutaneous infusion].

A continuous subcutaneous infusion of peplomycin was performed on 15 patients with cervical cancer. Eight patients received 5 mg of peplomycin a day and 7 patients received 2.5 mg a day. The peplomycin concentrating in the serum after infusion for 24 hours thus were 0.094 microgram/ml and 0.056 microgram/ml, respectively. The concentration of peplomycin in the tumor was found to be higher than in normal cervical tissue, and in the lymph nodes it was equal or higher than in the serum. Histological changes were minimum. A Grade IIa determination, however, was found in one patient, which may have been due to the total dose and/or the time of the examination. No pulmonary toxicity developed in any patients.

Adult↗
PubMed1988

[Comparison of methods for evaluating the nephrotoxicity of cisplatin].

The BUN, serum creatinine, creatinine clearance and the urinary excretion of leucine aminopeptidase (LAP), alkaline phosphatase (ALP), beta 2-microglobulin (beta-m), and N-acetyl-beta-glucosaminidase (NAG), were measured in 21 gynecological cancer patients treated with CAPF (CPA + ADM + CDDP + 5-FU) to evaluate the sensitivity of these indices to renal tubular damage. After receiving CDDP almost all patients displayed an increase in excretion of beta-m but no urinary enzyme activities. However, NAG index (NAG activity/urinary creatinine) rose markedly in all patients. We concluded that NAG index is a valuable method in providing sensitive indices for detecting renal tubular damage caused by CDDP.

Acetylglucosaminidase↗
PubMed1988

[High-dose CDDP therapy using a balloon-occluded arterial infusion (BOAI) in recurrent ovarian cancer].

An intraarterial high-dose CDDP, using a balloon catheter, has been infused into five patients with a recurrent ovarian cancer. The dose of CDDP was 150 mg-200 mg/body. Two patients experienced a partial tumor regression, whereas three showed no change. With regard to the toxicity, the NAG index (NAG activity/urinary creatinine) rose markedly in all patients, but toxicity was almost similar to that seen from a systemic administration. No significant catheter complication occurred.

Adult↗
PubMed1987

Changes of neurotensinlike immunoreactivity in the striatum of the cat after intrastriatal injection of kainic acid.

In the course of our study on the effects of kainic acid (KA) on neuropeptidelike immunoreactivity in striatal neurons, it was found that neurotensinlike immunoreactivity (NT-LI) became visible immunohistochemically in cell bodies of some striatal neurons after injecting KA stereotaxically into the head of the caudate nucleus (Cd) of the cat. The adult cats injected with KA were allowed to survive for 1-56 days, and their brains were examined by the indirect immunoperoxidase method. In the intact Cd, NT-LI was observed in axonal elements that accumulated to form irregularly shaped patches and displayed a discrete island-and-matrix pattern. At the site of KA injection, where neuronal cell loss and gliosis were produced. NT-LI almost disappeared by the fourth day after KA injection. In parallel with these changes, many neuronal cell bodies with NT-LI became visible in striatal regions surrounding the site of KA injection. Most of these neuronal cell bodies that showed NT-LI were medium-sized and extended thick dendrites with spines; spiny processes were rarely seen on the cell bodies. On the other hand, when a lesion was placed in the head of the Cd by thermocoagulation, no NT-LI could be seen in cell bodies of striatal neurons. Thus it was presumed that NT-LI in cell bodies of medium-sized, spiny neurons of the cat striatum might be at a low level under normal conditions but enhanced in cell bodies of the neurons that survived the KA injections.

Animals↗
PubMed1987

Direct projections from the caudal spinal trigeminal nucleus to the striatum in the cat.

The results of a WGA-HRP and HRP study in the cat indicated that some neurons in the marginal zone (lamina I) of the caudal spinal trigeminal nucleus sent their axons contralaterally to the striatum; mainly to the dorsal part of the putamen, and additionally to the ventrolateral part of the caudate nucleus, at the stereotaxic rostrocaudal levels of A 13.0-A 15.5.

Animals↗
PubMed1987

Thalamocortical and thalamo-amygdaloid projections from the parvicellular division of the posteromedial ventral nucleus in the cat.

Projections from the parvicellular division of the posteromedial ventral thalamic nucleus (VPMpc) of the cat were examined. After injection of horseradish peroxidase conjugated with wheat germ agglutinin (WGA-HRP) into the VPMpc, both anterogradely labeled axon terminals and retrogradely labeled neuronal cell bodies were found ipsilaterally in three discrete regions of the cerebral cortex, i.e., in the orbital cortex, caudoventral part of the infralimbic cortex, and medial part of the fundus of the posterior rhinal sulcus (perirhinal area); in the subcortical regions, anterogradely labeled axon terminals were seen ipsilaterally in the rostrodorsal part of the lateral amygdaloid nucleus. Neuronal connections between these VPMpc-recipient regions were further verified by injecting WGA-HRP into each of the three cortical and the lateral amygdaloid regions. After injection of WGA-HRP into each of the three cortical regions, labeled neuronal cell bodies and axon terminals were seen ipsilaterally in the VPMpc, especially in its medial part, and in the other two of the three VPMpc-recipient cortical regions. In the rostrodorsal part of the lateral amygdaloid nucleus, both axon terminals and neuronal cell bodies were labeled after WGA-HRP injection into the perirhinal area, and only axon terminals were labeled after WGA-HRP injection into the orbital cortex, but no labeling was observed after WGA-HRP injection into the infralimbic cortex. After injection of WGA-HRP into the rostrodorsal portion of the lateral amygdaloid nucleus, both axon terminals and neuronal cell bodies were labeled ipsilaterally in the perirhinal area and the ectorhinal area, and only neuronal cell bodies were labeled ipsilaterally in the VPMpc (especially in its medial part) and orbital cortical region; no labeling was observed in the infralimbic cortex. The present results indicate that the VPMpc of the cat is connected reciprocally with the orbital, infralimbic, and perirhinal cortical regions on the ipsilateral side, that the three VPMpc-recipient cortical regions are reciprocally connected with each other, that the VPMpc sends fibers ipsilaterally to the rostrodorsal part of the lateral amygdaloid nucleus, which may relay information from the VPMpc to the perirhinal cortical area, and that the VPMpc-recipient area in the lateral amygdaloid nucleus receives cortical fibers from the orbital and perirhinal cortical regions.

Amygdala↗
PubMed1987

Direct projections from the dorsal column nuclei and the spinal trigeminal nuclei to the cochlear nuclei in the cat.

A retrograde and anterograde wheat germ agglutinated horseradish peroxidase WGA-HRP study in the cat indicated that some neurons in the dorsal column nuclei and the interpolar and caudal spinal trigeminal nuclei send fibers to the dorsal and ventral cochlear nuclei; to the pyramidal cell layer of the dorsal cochlear nucleus and to the cochlear granule cell domain, bilaterally with an ipsilateral dominance.

Animals↗
PubMed1987

Direct projections from Ammon's horn to the septum in the cat.

Direct projections from Ammon's horn to the septum were studied in the cat by the anterograde tracing method after injecting WGA-HRP (wheat germ agglutinin-horseradish peroxidase conjugate) into Ammon's horn. The results were further confirmed by the retrograde WGA-HRP method after injecting WGA-HRP into the septum. Pyramidal neurons in fields CA1, CA2 and CA3 were observed to send their axons ipsilaterally to the lateral septal nucleus; the septal parts of the hippocampus sent projection fibers to the dorsomedial portions of the lateral septal nucleus via the medial aspects of the subcallosal fornix, while the hippocampal regions successively more proximal to the temporal pole sent projection fibers to progressively more ventrolateral portions of the lateral septal nucleus via more lateral aspects of the subcallosal fornix. It was also found that the septal parts of fields CA1, CA2 and CA3 sent projection fibers bilaterally to the dorsomedial aspects of the lateral septal nucleus. Field CA4 appeared to send projection fibers only sparsely, if at all, to the medial septal nucleus. The rudimentary parts of the hippocampal formation, taenia tecta and indusium griseum, were found to have reciprocal ipsilateral connections with the dorsal portions of the lateral septal nucleus.

Animals↗
PubMed1987

Efficacy of anticancer agents in vitro and in vivo using cultured human endometrial carcinoma cells--study of therapeutic index.

Employing the new cell line, NUE-1, which was derived from cells of ascites in a woman with endometrial carcinoma, the sensitivity test for anticancer agents was carried out in culture and xenografts in nude mice. Anticancer activity in vitro was evaluated by counting surviving cells, and the therapeutic index was expressed by LD50 for mice/MLD90 in vitro. NUE-1 cells were inoculated subcutaneously in BALB/c nude mice, and then tumors serially transplanted were used as materials. Anticancer agents (ADM, CDDP, CHA3, CQ, MMC) at 1/3 LD50 dosage for mice were administered intraperitoneally on a schedule of 3 doses for every 4 days. The results were as follows: The therapeutic index of ADM was highest at five to nineteen times the others In vivo, ADM demonstrated + activity, whereas others had no significant effect There was a close correlation between the therapeutic index and in vivo anticancer effect using nude mice.

Animals↗
PubMed1987

[Squamous cell carcinoma of the endometrium coexistent with synchronous adenocarcinoma of the cervix].

Primary squamous cell carcinoma of the endometrium is extremely rare. The patient reported here with squamous cell carcinoma of the endometrium and a synchronous adenocarcinoma of the cervix, represents an extreme, unique example of multiple Müllerian neoplasms. The 70-year-old female had a three-month history of vaginal bleeding. On July 13, 1984, she underwent a radical hysterectomy with a pelvic lymphadenectomy. A histological examination substantiated keratinizing squamous cell carcinoma of the endometrium this tumor extending outside the uterus, and an adenocarcinoma of the cervix. Post-operatively she received chemotherapy and irradiation therapy, but metastases reoccurred and she died on December 1, 1984.

Adenocarcinoma↗
PubMed1987

[Antitumor effects of the tumor necrosis factor (PAC-4D) against human gynecological carcinoma transplanted into nude mice].

The antitumor effects of human recombinant TNF (PAC-4D) were examined on three human gynecological carcinomas transplanted into CD-1 nude mice (uterine cervical carcinoma: UZ-1-N; ovarian carcinoma: OCl-1-N and OS-4-N). PAC-4D was administered intratumorally at a dose of 1,000 U, 3,000 U or 10,000 U/head from day 0 to day 4. Tumor size, body weight and peripheral WBC were measured on days 0, 4, 8, 12 and 16 and histological studies were made on day 6. The results were as follows: With UZ-1-N, intratumoral administration of PAC-4D at doses of 3,000 U and 10,000 U/head caused a marked inhibition of the tumor growth. Similarly, antitumor activity of PAC-4D against OCl-1-N was remarkable at a dose of 10,000 U/head. The administration of PAC-4D at doses of 3,000 U and 10,000 U/head was not effective against OS-4-N. The histological changes of grade II A-B by Ohboshi-Shimosato criteria of response were observed in the tumor of the effective groups against PAC-4D. There were no influences on the body weight and WBC after administration of PAC-4D. Although tumor cells possessed different sensitivities to PAC-4D, PAC-4D is strongly recommended for the treatment of gynecological malignancies.

Animals↗
PubMed1987

Establishment and characterization of the cell-line of a human endometrial carcinoma.

A new cell line of a human endometrial carcinoma, designated as NUE-1, has been established in culture and maintained for more than five years. The cell line has a doubling time of 40 h and grows as a confluent monolayer together with a constant population of free-floating cells. Electron microscopically, NUE-1 cells showed poorly differentiated features. Chromosome analysis revealed hyperdiploidy and two marker chromosomes. When cells from passage 6 were injected subcutaneously into nude mice, tumors developed. The histological pattern was indistinguishable from that of the original tumor, and was undifferentiated carcinoma. The cells contained no estrogen or progesterone receptors.

Cell Division↗