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Biomedical subjects

Y Wu

Publications and source records attributed to Y Wu.

At least 559 records · Page 31Linked to original sources
PubMed1999

[MR urography in the diagnosis of upper urinary tract obstruction].

OBJECTIVE: To evaluate the role of magnetic resonance urography (MRU) in the diagnosis of upper urinary tract obstruction. METHODS: MRU was performed for 35 patients suspicious of upper urinary tract obstruction. RESULTS: MRU provided high resolution images of the urinary tract. The site of upper urinary tract obstruction and hydronephrosis could be clearly shown. CONCLUSIONS: MRU, a nontraumatic technique, needs no iodinated contrast medium. It might be widely adopted in the diagnosis of upper urinary diseases.

Adolescent↗
PubMed1999

[External adrenal pheochromocytoma].

OBJECTIVE: To improve the diagnosis of external adrenal pheochromocytoma. METHODS: From 1958 to 1998, 66 cases of external adrenal pheochromocytoma, 23.3% of pheochromocytomas, at the same period in the Department of Urology, RuiJin Hospital were reviewed. RESULTS: In the 66 cases, the malignant rate was high (33%). CONCLUSIONS: (131)I-MIBG is important in the diagnosis and treatment of external adrenal pheochromocytoma. Long-term follow up after operation is imperative to the cases of external adrenal pheochromocytoma.

3-Iodobenzylguanidine↗
PubMed1999

[Responses of normal rat kidney to different ischemic ways].

OBJECTIVE: To evaluate the effects of different ischemic ways on rat kidneys. METHODS: Male Wistar rat kidneys were divided into sham operation (group A), continuous ischemia (group B), intermittent ischemia (group C), ischemic preconditioning (group D), and delayed effect of ischemic preconditioning group (group E). The functional and morphologic changes and levels of MDA of the kidneys which suffered from different ischemia were assessed. RESULTS: The functional indexes of the group C were similar to that of the sham group. In the group B, D and E, the rats showed acute renal failure. Morphological findings showed that the groups B, D and E had severe tubular necrosis (+ + + - + + + +) 24 hours after operation. The group C showed light tubular necrosis (+). 30 minutes after reperfusion, the level of MDA was significantly lower in the group C than in the groups B, D and E (P < 0.01). CONCLUSIONS: In a given ischemic period, the injury effect of intermittent ischemia-reperfusion was much lower than that of continuous ischemia-reperfusion. Experimentally, ischemic preconditioning did not have protective effect on rat kidneys, including "classic protection" and "protection of second window". Intermittent ischemia-reperfusion did not increase OFR and lipid peroxidation injury.

Animals↗
PubMed1999

[Studies of the mechanism of lowering intraocular pressure on Erycibele alkaloid].

OBJECTIVE: To investigate the molecular pharmacological mechanism of lowering intraocular pressure of Erycibele alkaloid. METHODS: Rabbit pupil diameter was measured by a pupillary ruler in constant illumination and the intraocular pressure (IOP) was measured by pneumatonometer. Iris contracting force was measured in an experiment of isolated rabbit iris. Radioimmune method was used to determine the content of cyclonucleotide. RESULTS: The pD(2) value (the affinity to M-receptors of agonist) of Erycibele alkaloid's miosis is 3.60 +/- 0.15 and its IOP lowering action is 3.49 +/- 0.07. In the isolated iris contracting experiment, its pD(2) value is 6.38 +/- 0.12. M(3) receptor antagonist is the strongest antagonist in inhibiting the miosis, IOP lowering action and isolated iris pupillary contracting action of Erycibele alkaloid. 0.01% Erycibele alkaloid can cause the decrease of cAMP and the increase of cGMP in the aqueous humor. CONCLUSIONS: The effects of miosis and IOP lowering of Erycibele alkaloid are all mediated by M(3) receptor subtype and its signal transductive mechanism is connected with cyclic nucleotide system.

Animals↗
PubMed1999

[The experimental research of purification and character of cultured retinal ganglion cell].

OBJECTIVE: To establish a cell line and purification model of retinal ganglion cells (RGCs) in vitro. METHOD: RGCs from Sprague Dawley neonatal rats (postnatal 1 - 3 days) were cultured in basal medium eagle (BME) basal medium. The growth regularity of RGCs in vitro was observed under phase-contrast microscope. RGCs were purified by Thy 1.1 with FITC antibody and detected under fluorescent microscope and phase-contrast microscope. RESULTS: Higher density of retinal cells and tectal extract facilitate cultured RGCs to survive. The purification rate of retinal ganglion cells in the experiment arrived at 95 percent. CONCLUSION: Cytokine and trophic factors from other cells in the retina and tectal extract can promote RGCs to survive, and they can be purified by Thy 1.1 antibody.

Animals↗
PubMed1999

[The correlation of free radicals induced by cadmium toxicess with erythrocyte injury and band3 expression].

OBJECTIVE: To study the correlation of hydrogen peroxide induced by cadmium ion with erythrocyte injury and band3 expression. METHODS: It was application immunoscanning electronmicroscopy and cytochemistry and EDX methods. RESULT: Cadmium ion not only induced a lot of hydrogen peroxide but also caused serious distortion of erythrocyte. CONCLUSION: Cadmium ion-induced free radicals lead to oxygen free radical damage of membrane and crytoskelecton of erythrocyte, affecting the stability and deformability and band3 expression in erythrocyte.

Animals↗
PubMed1999

[Application of cerium cytochemical method in observation of renal injury caused by oxygen free radical with cadmium induction].

OBJECTIVE: To study acute renal injury caused by oxygen free radical with cadmium induction. METHODS: Electron microscopy cytochemical and energy dispersive X-ray (EDX) analysis techniques were used. RESULTS: Cadmium ions could enter the epithelial cells of renal proximal convoluted tubules. Excessive production of oxygen free radical was found in the kidney tissues, which located mainly in the endothelial cells of blood vessels, mitochondria of epithelial cells of renal proximal convoluted tubules, microvilli and basement membrane. Swelling of epithelial cells and mitochondria and drop of epithelial cells could occur in renal proximal convoluted tubules. CONCLUSION: It suggests that cadmium ions can induce the formation of large amount of oxygen free radical and cause renal injury.

Acute Kidney Injury↗
PubMed1999

[Studies on appropriate methods for dust scrubbing from dust-laden gas in shaft kilns of small-sized cement plants].

OBJECTIVE: To study an appropriate method for dust scrubbing from dust-laden gas in the shaft kilns of small-sized cement plants. METHODS: Characteristics of dust-laden gas, including its temperature, dew point, dust concentration, diameters of dust particles and their distribution, as well as fractional dust collection efficiencies of the dust precipitator settling chamber, cyclone, impinging scrubber, impinging-spray scrubber, were measured according to the National Standards for Measurement of Particulate in Exhaust Gas Emitted from the Stationary Pollution Sources and Sampling Methods of Gaseous Pollutants (GB/T16157 - 1996), with a WY-1 In-stack 7-Stage Cascade Impactor for fractional dust collection. RESULTS: The fractional dust collection efficiency of the settling chamber for the particles greater than 12 microm was 72%, while that of the cyclone for the particles of 2 microm, 5 microm and 10 microm were 40.0%, 72.5% and 88.9%, respectively, and that of impinging scrubber was 55.0%, 91.0% and 98.9%, respectively; and that of impinging-spray scrubber were 96.0%, 99.2% and 99.6%, respectively. Results showed that 81% of the dust (by weight) from the shaft kilns were greater than 10 microm, so the settling chamber was effective for the removal of this fraction of dust. But dust particles behind the settling chamber were finer, and about 59% - 69% of them were less than 2 microm in diameters. In that case, the cyclone, impinging scrubber and impinging-spray scrubber could give an overall collection efficiency of 54%, 57% and 96%, respectively. Based on this, the following combination of dust collectors were adopted to meet the national standards for exhaust emission: a settling chamber plus an electrostatic precipitator, a settling chamber plus an impinging-spray scrubber and a settling chamber plus an impinging scrubber. The cement plants could select appropriate dust collectors according to their technical and economical conditions. CONCLUSION: Based on the results of the studies, appropriate dust collectors used to treat dust-laden gas from shaft kilns to meet the national standards for exhaust emission were recommended.

Air Pollutants, Occupational↗
PubMed1999

[The mechanism of p16 gene inactivation in human bladder transitional cell carcinoma].

OBJECTIVE: To figure out the mechanism of p16 gene inactivation in human bladder transitional cell carcinoma (BTCC). METHODS: p16 gene was analyzed for genetic alterations by Southern blot analysis, PCR-SSCP and DNA methylation analysis in 52 cases of transitional cell carcinoma of bladder. RESULTS: p16 gene deletion was found in 11 of 52 (21.15%) BTCCs, only one tumor showed point mutation on p16 exon 2, nineteen cases of BTCCs (36.53%) showed p16 gene 5' CpG island methylation. CONCLUSION: There was a statistically significant association between p16 gene deletion and early stage, well differentiated tumors. Additionally, a statistically significant correlation was also noticed between p16 gene 5' CpG island methylation and tumors in late stage with poor cell differentiation.

Blotting, Southern↗
PubMed1999

[Efficacy of TACE in treatment of intraportal tumor thrombi with double helical CT].

The present study observed alterations of the intraportal tumor thrombi before and after transcatheter hepatic arterial chemo-embolization(TACE) in 36 patients with massive hepatocellular carcinoma(HCC). Elscint double helical CT was used to scan liver in the portal phase before and after TACE. The results showed that the scan technique could be used to evaluate the distribution of intraportal tumor thrombi. After treating with TACE, retention of iodized oil was found in the intraportal tumor thrombi(14/36), and the intraportal tumor thrombi were reduced in 28 out of 36 cases. The survival rate was more than 1 year in 20 patients. These data indicate that the blood supply of the intraportal tumor thrombi comes from hepatic arterial system. TACE seems to be valuable for the treatment of intraportal tumor thrombi with massive HCC.

Adult↗
PubMed1999

[PCR for the detection of 16S rRNA gene bacteria].

According to the high conservative region of 16S rRNA gene in bacteria, PCR primers of the broad-range bacteria, gram-positive bacteria and gram-negative bacteria were synthesized to detect 13 bacterium species and (40) clinical specimens. All the tested bacterium species were positive. The lowest concentration of Escherichia Coli detected by serial dilution was 4 CFU.ml-1. The positive rate of PCR (27/40) was higher than that of bacterium culture(18/40). The results indicate that these PCR primers possess high specificity and sensitivity in identifying 16S rRNA gene of bacteria.

Bacteriuria↗
PubMed1999

[Study on the isolation method for the different states of atmospheric pollutant].

An annular denuder system(ADS), which consisted of denuder tubes striping the reactive gases from air and leaving the particles on the filters was designed. After sampling, the absorbed gases and particles were separately leached with water and determined by ion chromatography. The detection limit of samples was about 1.0 x 10(-4) mg/m3 for SO2, HNO2, SO4(2-) and NO3-. The loss of particles on the denuder faces was less than 5% and the collection effectiveness of particles was 100%. Absorbtion effectiveness for gases was more than 95%. The average RSD was less than 6.5%.

Air Pollutants↗
PubMed1999

[The synthesis of multifunctional groups and multibranched compounds: IX. Synthesis of several branched building block].

The branched building plays a pivotal part in the study of Dendrimers. In order to synthesized Dendrimers more effectively, we designed and synthesized four new branched building blocks via 2-4 steps starting from diethyl malonate and pentaerythritol. Their structures were confirmed by IR and 1HNMR etc. They compare favourably with previous branched building blocks as regards to steric hindrance and branched effect.

Malonates↗
PubMed1999

[Influence of different total parenteral nutrition support upon hepatic regeneration after partial hepatectomy in rats].

This study was designed to investigate the effects of different TPN on liver regeneration after partial hepatectomy in rats. Forty Wistar rats were randomly divided into four groups. Groups A and B (normal rats) received BCAA-enriched TPN (45% BCAAs) and standard TPN (24% BCAAs) respectively after 34% hepatectomy; Groups C and D (rats with cirrhosis of liver) received the same TPN as Groups A and B received respectively after 34% hepatectomy. On the third day after operation, all rats were killed and specimens of liver and blood were taken for measurement of activity of liver regeneration. The results showed that DNA synthesis rate and mitosis rate in Groups A and B were significantly greater than those in Groups C and D respectively; RLR, DNA synthesis rate and mitosis rate in Groups A and C were significantly higher than those in Groups B and D respectively. These suggest that the effect of the same TPN on liver regeneration in normal rats is better than that in cirrhosis rats and that BCAA-enriched TPN can provide more excellent supplementation than standard TPN can do either in normal liver or cirrhosis liver after partial hepatectomy.

Animals↗
PubMed1999

[Assay of aluminum content in alum and alum-processed medicinal herbs and its distribution in blood and brain in mice].

OBJECTIVE: To assay the aluminum content in alum and some Chinese medicinal herbs processed with alum, and compare the aluminum distribution of these herbs in blood and brain in mice. METHOD: Aluminum content of the herbs was assayed with spectrophotometry, snd aluminum distribution in blood and brain was assayed with atomic absorption spectrophotometry. RESULTS: Aluminum content in alum was found higher than that in the alum-processed herbs. Aluminum distribution in blood and brain after oral administration at equimolecular doses, equivalent to 5.7 mg/kg of metal Al, was studied in mice, and no significant differences were observed. CONCLUSION: Aluminum distribution in blood and brain in mice is related to the dosages of aluminum administered and has little to do with the kind of herbs used. All these medicinal herbs do not improve the absorption of aluminum in mice.

Alum Compounds↗