Biomedical subjects

Y Wu

Publications and source records attributed to Y Wu.

At least 577 records · Page 32Linked to original sources

PubMed1999

[Long-term results of surgical treatment of intractable Menière's disease for control of vertigo].

OBJECTIVE: To acquire the long-term (an average of over 10 years follow-up) results of surgical treatment of patients with Meniere's disease for control of vertigo. METHOD: Endolymphatic sac surgery was done in 6 cases (1 case of endolymphatic sac decompression and 5 cases of sac-mastoid shunting) and translabyrinth vestibular neurotomy was operated on 6 cases. RESULT: Complete relief from symptoms of vertigo occurred in the 6 cases through vestibular neurotomy and there were no recurrence in 11-13 years follow-up. Three of 6 cases with endolymphatic sac surgery had complete control of vertigo and were cured clinically. Symptoms of vertigo of the rest 3 cases were effectively controlled. CONCLUSION: Endolymphatic sac surgery and translabyrinth vestibular neurotomy are effective way for control of vertigo of patients with intractable Meniere's disease. Translabyrinth vestibular neurotomy are more effective for control of vertigo for appropriately selected patients.

Adult↗

PubMed1999

[Clinical analysis of 28 patients with unsuccessful septoplasty].

OBJECTIVE: To analyse the causes of unsuccessful septoplasty. METHOD: 28 cases were investigated by high-resolution CT scan and nasal endoscopy. Another operation was completed in endoscopic sinus surgery. RESULT: 16 upper or posterior nasal septal deviation and 12 paraseptal structural deformities and chronic sinus diseases were observed, which were neglected in the first operation. CONCLUSION: Before septoplasty, CT and endoscopic evaluation of the paranasal sinuses and paraseptal structures should be performed and accompanied nasal diseases should be considered.

Adolescent↗

PubMed1999

[The clinic features of human immunodeficiency virus (HIV) on ear, nose, throat head and neck].

OBJECTIVE: To identify the clinic features of HIV infection on ENT-Head and neck area and to explore its treatment. METHOD: 68 HIV cases in zambia were analysed. RESULT: The ages of the patients are from 3 months to 46 year old. Among them 12 cases with Lump of neck, 9 with ulcer of oral-pharynx, 9 with parctid gland enlarged, 2 with submaxillary plleymon, 5 with nasal bleeding, 17 with purulent otitis Media, 4 with atrophic rhinitis. 18 cases in 58 HIV patients had tubericulosis, diarhea and anemia. 51 cases improved after treatment while 15 cases didn't improve and 2 died. CONCLUSION: It is very important to find and treat HIV patients and to prevent transmission as early as possible.

Adolescent↗

PubMed1999

[Determination of chlorogenic acid, glycyrrhizin and glycyrrhetinic acid in yinqiaojiedupian by high performance capillary electrophoresis].

A simple, rapid and reproducible capillary electrophoretic method was developed for the determination of chlorogenic acid, glycyrrhizin and glycyrrhetinic acid in Yinqiaojiedupian. The buffer solution used in this method was a mixture of 20 mmol/L NaH2PO4 and 5 mmol/L Na2B4O7(pH 7.0). The other conditions were as follows: applied voltage, 20 kV (positive to negative polarity); L = 60 cm; Leff = 55 cm; i.d. = 75 microns; lambda = 254 nm; temperature, 25 degrees C; injection time, 5 s hydrostatic. The linear calibration range was 1.568-100.4 mg/L for glycyrrhetinic acid, 8.000-128.0 mg/L for glycyrrhizin and 8.080-129.3 mg/L for chlorogenic acid, and recoveries were 98.0%-103% for glycyrrhetinic acid, 97.3%-102% for glycyrrhizin and 97.3%-100% for chlorogenic acid. The relative standard deviations (n = 3) were 0.2%-1.3% (intra-day) and 1.9%-3.0% (inter-day) for glycyrrhetinic acid, 0.5%-1.2%(intra-day) and 1.9%-2.1%(inter-day) for glycyrrhizin and 0.7%-1.3%(intra-day) and 1.6%-2.1%(inter-day) for chlorogenic acid. The contents of these three acids in Yinqiaojiedupian were successfully determined within 10 min.

Anti-Infective Agents↗

PubMed1999

[A new viscoelastic model for granulocytes].

In this paper, a new viscoelastic model for granulocytes was introduced. The stress was placed on the theory and its experimental results of three-parameter viscous model with micropipette aspirating technique. The small deformation of single rat granulocyte into the micropipette was analyzed by the model. Within the small deformation, the fit of the experiment data using the three-parameter viscous model was more satisfactory than that using the standard solid model, and the former suggested that cytoplasm of granulocyte was treated as a liquid rather than a solid.

Animals↗

PubMed1999

[The advances of research for problems of developing flow in blood vessel].

The development and the tendency of the problems of developing flow in the vessel are reviewed in this paper. It is pointed out that the problems of developing flow of viscoelastic blood vessel are front subjects, and that consideration shouldbe be give to three aspects, namely the blood flow, the blood vessel's wall motion and the coupling between the blood and the wall.

Blood Flow Velocity↗

PubMed1999

[First discovery of Taenia saginata asiatica infection in Yunnan province].

AIM: To investigate the aetiology, species and epidemiological factors of Taenia infection in a pilot area of Lanping County, Yunnan Province. METHODS: Two patients with taeniasis were treated with antiscolex capsule and praziquantel, respectively. Five local weaning pigs free from tapeworm infection were fed with gravid proglottids obtained from the patients. After 2-3 months, the pigs were necropsied to examine cysticerci. In addition, one pig naturally infected with Taenia was treated as well. RESULTS: Based on morphological characteristics of the scolex and gravid proglottid of four tapeworms obtained from the patients, the worms were similar to T. saginata. 23 cysticerci recovered from two experimentally infected pigs were found in the livers, omentum majus and mesenterium, and 3 cysticerci were found in the liver and omentum majus in a naturally infected pig as well. The protoscolex of mature Cysticercus had two rows of rudimentary hooklets, one rostellum and four acetabula resembling to C. cellulosae. According to morphological characteristics and the location of cysticerci, the tapeworm was identified as Taenia saginata asiatia. CONCLUSION: T. saginata asiatica infection was first reported in Yunnan Province.

Adult↗

PubMed1999

[Diagnosis and prognosis evaluation of primary non-Hodgkin's lymphoma of the nose].

OBJECTIVE: To Study the diagnosis of primary nasal non-Hodgkin's Lymphoma (NHL) and to find the relationship between its classification, stage and prognosis. METHOD: The data of 10 cases with primary nasal NHL documented pathologically were analysed retrospectively. RESULT: The classification and stage were related to the prognosis significantly. Following up seven cases, 5T-ML with severe systemic symptoms died 4-6 months after diagnosis and treatment. One earlier T-ML is survival till now, and another B-ML was alive for 2.5 years. CONCLUSION: Early diagnosis of NHL of nose based on pathology is very important for treatment and prognosis. Repeat biopsies will be necessary when the diagnosis is suspected. The prognosis of late T-ML is disastrous.

Adolescent↗

PubMed1999

[Relationship between complement activation and reactive oxygen species generation in inflammatory response].

OBJECTIVE: To verify the proposed activation feedback mechanism between complement and polymorphonuclear leukocytes(PMN) derived reactive oxygen species(ROS). METHODS: In vitro tests were designed to identify the activation feedback process by alternative activations of complement by inulin or PMNs by 12-O-tetradecanoylphorbol-13-acetate(TPA) in the test medium in consisting serum complement and isolated PMNs, with chemiluminescence technique to determine the production of ROS and complement fixation trial for complement activations. RESULTS: Evidently that activated complement could activate PMNs to release ROS which further activated complement, so the activating feedback cycle was set. CONCLUSIONS: The complement and PMNs-ROS activation feedback cycle mechanism was recognized. It was expected to serve for delineating the regulation of inflammatory response.

Animals↗

PubMed1999

[Apoptosis of human pancreatic carcinoma cell lines induced by combinations of chemotherapeutic drugs].

OBJECTIVE: To investigate the apoptotic in human pancreatic carcinoma cell lines P3, SW1990 and Capan-2 induced by the combined treatment of 5-fluorouracil (5FU), mitomycin C (MMC), carboplatin(CBP) or adriamycine (ADM) or epirubicin (E-ADM). METHODS: The changes of cell morphology were monitored by means of microscopic and fluorescence technique after treated with the combinations of chemotherapeutic drugs. The rate of apoptosis was calculated with flow cytometry and the fragmentation of DNA was measured by agarose gel electrophoresis. RESULTS: The combinations of chemotherapeutic drugs were able to induce the apoptosis of human pancreatic carcinoma cell lines. Compared with the cell necrosis, the dosage of drugs was less and the time was shorter for inducing apoptosis. With the increased dosage and/or the time prolonged for treating the cells, the rate of apoptotic cells was increased. However, the apoptosis reaction of various human pancreatic carcinoma cell lines, which were mentioned above, induced by the same combinations of chemotherapeutic drugs were various different. CONCLUSIONS: The apoptosis process might be a mechanism of an inhibiting cancer cell growth induced by the combinations of chemotherapeutic drugs. The examination of apoptosis reaction for the cells induced by different methods and/or chemotherapeutic drugs could become an important index of testing their ability of inhibiting pancreatic neoplasm.

Antineoplastic Combined Chemotherapy Protocols↗

PubMed1999

Distribution of human lens crystallins and their sulphydryl contents of different age in two-dimension electrophoresis.

PURPOSE: To analyze water-soluble (WS) human lens proteins of fetus, adult and age-related cataract by two-dimensional IEF/SDS-PAGE electrophoresis. METHODS: DACM [N-(7-Dimethylamino-4-methyl-3-coumarinyl) maleimide] was used to determine the lens proteins sulphydryl (SH) content. RESULT: Protein SH contents in WS lens proteins have no significant difference among fetus, adult and age-related cataract lens. This is different from the relative published results obtained in lens proteins of animal cataract model using similar SH detecting methods. CONCLUSIONS: IEF/SDS-PAGE electrophoresis demonstrated that there were much more fragmentation of crystallins during lens development and cataractogenic process. It is suggested that this phenomenon is likely to be due to further conformational changes in the fragmented cyrstallins during aging and cataractogenic process.

Adult↗

PubMed1999

An experimental study on homoharringtonine liposome and glaucoma filtration surgery.

PURPOSE: To observe the inhibitory action of homoharringtonine liposome during the healing process of wounds in the filtering sites. METHODS: Posterior sclerectomies were performed in 14 rabbits. Postoperatively one eye of each rabbit received subconjunctival injections of HH liposome and fellow eye received saline injection in a randomized masked fashion. RESULTS: Fourteen days after operation the IOP of experimental eyes reduced significantly (P < 0.01) as compared with the controlled eyes, and the number of remaining filtering blebs increased noticeably (P < 0.05). Pathohistological examination revealed that the number of fibroblasts per square micron in the filtering sites and the thickness of the scars in the center of the filtering sites of the experimental eyes were less than those of the controlled eyes. No serious ocular toxic and side effects were found. CONCLUSION: This experiment suggest that homoharringtonine liposome can markedly inhibit the scar formation of filtering sites after glaucoma filtering surgery, promote the formation of filtering blebs, and provide an experimental evidence for its clinical use in future.

Animals↗

PubMed1999

[Effect of N-methyl-D-aspartate and dexamethasone on apoptosis of rat cultured retinal ganglion cells].

PURPOSE: To investigate the effect of N-methyl-D-aspartate(NMDA) and Dexamethasone on cultured rat retinal ganglion cells (RGCS). METHODS: RGCs were obtained from 1-3 days old SD rats. In two groups, exposure of cocultured ganglion cells to N-methyl-D-aspartate (20-500 mumol/L) lasted for 24 hours. Apoptotic cells were identified by Hochest 33258 in one group; In another group, 0.4% Trypan blue dye was added. Cells excluding the dye were counted, and the survival rate of cells was determined by the ratio of the excluding cells in the experiment over in the control; Cocultured RGCs and purified RGCs were exposed to Dexamethasone(1 x 10(-4), 1 x 10(-5) and 1 x 10(-6) mol/L). After 24 hours, apoptotic cells were identified by Hochest33258. RESULT: Cocultured RGCs showed distinct morphological appearance of apoptotic cells when they were exposed to NMDA. The survival rate of cells was dose-related to the concentration of NMDA. Cocultured RGCs didn't show typical apoptotic appearance at 24 hours after exposure to Dexamethasone. But purified RGCs did so, even in the control group. CONCLUSION: NMDA has the effect of inducing cocultured RGCs apoptosis. Dexamethasone had no this effect on RGCs. Without additional neurotrophic factors, purified RGCs may become apoptotic cells when cultured in vitro for 24 hours.

Animals↗

PubMed1999

[A new digital filter method for eliminating 50Hz interference from the ECG].

The article discusses advantages and disadvantages of several conventional digital filter methods. Then, based on Levkov method, the author proposed a new filter method. We use these methods to remove 50 Hz interference from more than 50 persons' ECG signals. The results show that this new method is the best, and it can satisfy the real time requirement of digital ECG machine.

Algorithms↗

PubMed1999

[The absorption spectra of crystal thin film Cs3Cu2I5].

In this paper we measured the absorption spectra of crystal thin film Cs3Cu2I5 at room and liquid nitrogen temperatures, respectively, and calculated the excitonic coefficient of the material. The excitonic coupling energy deltaE(ex)(1) = (0.53 +/- 0.07) eV, excitonic radius a(ex) = 0. 326 nm,and forbidden zone width E(g) = (5.00 +/- 0.07) eV. The spectral analysis shows that the electron and exciton excitations of the Cs3Cu2I5 localize in the CuI subcrystal lattice, and reveals the law that governs the variance of the first excitonic peak position of the serial Cs(x)Cu(1-x) I compounds with their mol-components at low temperature.

English Abstract↗

PubMed1999

[Spectroscopic study on the structure and orientation of 1-hydroxyl-5-dodecyloxy-naphthalene LB films].

Ultraviolet-visible (UV-Vis), infrared transmission reflection absorption (RA) and ATR spectra were used to study the mono-and multi-layer Langmuir-Blodgett (LB) films of C12H25ONaph (1,5)OH at various temperatures to investigate dependencies of the aggregation and phase transitions on the number of monolayers. The UV-vis spectra of the LB films revealed that C12H25ONaph (1,5)OH forms aggregates in the LB film. From the temperature dependence of UV-vis absorption spectra, it is clear that the aggregate was gradually converted to monomer with the temperature increased. A comparison of the infrared transmission and ATR spectra reveals that the hydrocarbon chains were tilted considerably in the LB film.

English Abstract↗

PubMed1999

[Synthesis and ion recognition function of 7-methoxy-4-methene-coumarin-monoaza-18-crown-6 (I) synthesis and spectroscopic properties].

The title reagent MMC-MAC (O5) is synthesized and characterized. Its acidic dissociation constant at ground and excited state are determined based on the changes of the absorption and fluorescence spectra in the water. According to the wavelength shift of fluorescence spectra in different solvent, the dipole moments of ground and excited state are estimated based on the molecular mechanics model using a alchemy 2000 software and the solvachromic method using the Lippert equation,the value is 3.11D and 8.13D,respectively. It's also found that fluorescence intensity of MMC-MAC(O5) aqueous solution is increased marvelously about twenty times and accompanying with delay fluorescence emit when deoxygenation from the solution by H2SO3-N+ (CH3) 4OH-. The intensity of normal fluorescence and delay fluorescence are proportional to the concentration of MMC-MAC(O5) in the range of 4 x 10(-8)-2 x 10(-6) and 2 x 10(-7)-2 x 10(-6) mol x L(-1), and the detection limits are 2 x 10(-9) and 1.3 x 10(-8) mol x L(-1) respectively.

English Abstract↗

PubMed1999

Effects of qigong on late-stage complex regional pain syndrome.

CONTEXT: Despite the growing popularity of qigong in the West, few well-controlled studies using a sham master to assess the clinical efficacy of qigong have been conducted. OBJECTIVE: To study the effect of qigong on treatment-resistant patients with late-stage complex regional pain syndrome type I. DESIGN: Block-random placebo-controlled clinical trial. SETTING: Pain Management Center at New Jersey Medical School. PATIENTS: 26 adult patients (aged 18 to 65 years) with complex regional pain syndrome type I. INTERVENTIONS: The experimental group received qi emission and qigong instruction (including home exercise) by a qigong master. The control group received a similar set of instructions by a sham master. The experimental protocol included 6 forty-minute qigong sessions over 3 weeks, with reevaluation at 6 and 10 weeks. Assessment included comprehensive medical history, physical exam, psychological evaluation, necessary diagnostic testing. Symptom Check List 90, and the Carleton University Responsiveness to Suggestion Scale. MAIN OUTCOME MEASURES: Thermography, swelling, discoloration, muscle wasting, range of motion, pain intensity rating, medication usage, behavior assessment (activity level and domestic disability), frequency of pain awakening, mood assessment, and anxiety assessment. RESULTS: 22 subjects completed the protocol. Among the genuine qigong group, 82% reported less pain by the end of the first training session compared to 45% of control patients. By the last training session, 91% of qigong patients reported analgesia compared to 36% of control patients. Anxiety was reduced in both groups over time, but the reduction was significantly greater in the experimental group than in the control group. CONCLUSIONS: Using a credible placebo to control for nonspecific treatment effects, qigong training was found to result in transient pain reduction and long-term anxiety reduction. The positive findings were not related to preexperimental differences between groups in hypnotizability. Future studies of qigong should control for possible confounding influences and perhaps use clinical disorders more responsive to psychological intervention.

Adolescent↗