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Biomedical subjects

Y Tanizaki

Publications and source records attributed to Y Tanizaki.

At least 127 records · Page 7Linked to original sources
PubMed1984

Histamine release from whole blood induced by anti-IgE: relationship to patient age, age at onset and serum IgE levels.

Anti-IgE-induced histamine release from basophils was examined in 46 asthmatic subjects using a whole blood method. Basophils from subjects less than 30 years old released more histamine than those from subjects aged 41 to 50. The age at onset of the disease also affected the reactivity of basophils to anti-IgE: basophils showed a high response in subjects whose age at onset was between 0 and 10 years, and low response in the subjects whose age at onset was between 41 and 50 years. There was a correlation between histamine release and serum IgE levels. However, individual dose-response curves of histamine release varied greatly in whom serum IgE levels were low.

Adolescent↗
PubMed1983

New in vitro method for detecting asthma allergen. Counting reactive basophils after addition of allergen.

Changes in the morphology and number of basophils were examined after the addition in vitro of house dust extract in cells from seventy-seven patients with bronchial asthma. Morphological changes (reactive basophils) showed a close relation to the end-point titrations of antigen in skin tests, RAST score and bronchial provocation tests, but changes in number of basophils did not. With a RAST score of 2 or greater the change in reactive basophils varied from 40 to 80% (mean 58.1%), which was much greater than seen in persons with no RAST, score 0. Patients with positive bronchial inhalation tests, showed 40 to 80% (mean 57.8%) reactive basophils, whereas persons with a negative bronchial challenge test showed a range of reactive basophils from 0 to 50% (mean 28.9%). The difference in percentage of reactive basophils between the positive and negative groups of persons tested by bronchial inhalation was statistically significant.

Allergens↗
PubMed1983

Effect of BSA on Ca2+ influx in mast cells stimulated by ovalbumin.

To examine the effect of bovine serum albumin (BSA) on the cell surface of mast cells, we measured 45Ca influx in mast cells from actively sensitized rats, and in cells passively sensitized in vitro with IgE-containing serum before or after BSA purification. Mast cells from actively sensitized rats showed a marked increase in 45Ca uptake after stimulation by ovalbumin. The uptake of 45Ca by mast cells passively sensitized before separation through BSA was also increased in response to ovalbumin. Variation in 45Ca uptake increased in the cells sensitized after separation. We did not observe a significant increase in 45Ca uptake in mast cells incubated with inactivated or normal serum compared to that of unstimulated cells.

Animals↗
PubMed1983

Inhibitory effect of nifedipine and cromolyn sodium on skin reactions and 45Ca uptake and histamine release in rat mast cells induced by various stimulating agents.

The effect of nifedipine, one of the calcium channel antagonists, was examined in allergic and nonspecific reactions using rat skin and peritoneal mast cells. Rat passive cutaneous anaphylaxis response mediated by IgE antibody was inhibited by nifedipine (100 micrograms/rat) and also by cromolyn sodium (5 mg/rat). Skin reactions induced by histamine or methacholine were not inhibited, and skin reaction induced by serotonin was slightly inhibited by these drugs. The 45Ca uptake and histamine release in sensitized mast cells stimulated by ovalbumin were suppressed by nifedipine. In actively sensitized cells, nifedipine seems to be more effective on the inhibition of 45Ca uptake, but less effective on histamine release than cromolyn sodium. In mast cells, nonimmunologically stimulated by concanavalin A, compound 48/80, and calcium ionophore A23187, nifedipine exerted considerable inhibitory effects on both 45Ca and histamine release.

Animals↗
PubMed1983

Inhibitory effect of the CA2+ antagonist nifedipine on histamine release from rat peritoneal mast cells.

45Ca uptake and histamine release was examined in mast cells from rats sensitized with ovalbumin and Bordetella Bertussis as an adjuvant. The uptake of 45Ca by the mast cells was significantly increased by stimulation with ovalbumin as was the release of histamine from the mast cells. Nifedipine, a calcium antagonist, inhibited the increase in both 45Ca uptake and histamine release stimulated by ovalbumin, though the effect on 45Ca uptake was stronger than that on histamine release.

Animals↗