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Biomedical subjects

Y Takeuchi

Publications and source records attributed to Y Takeuchi.

At least 865 records · Page 48Linked to original sources
PubMed1987

Isolation of human immunodeficiency virus from a Japanese hemophilia B patient with AIDS.

Human immunodeficiency virus (HIV) was isolated from a Japanese hemophilia B patient with AIDS. This isolate, HIV[GUN-1], was infectious to several mature T-cell lines. Proteins with apparent molecular weights of 160, 55 and 25 kilodaltons were detected. Restriction enzyme cleavage patterns of the proviral genome indicated that HIV[GUN-1] is related to but clearly different from HTLV-III or ARV-2.

Acquired Immunodeficiency Syndrome↗
PubMed1987

Increased lactate dehydrogenase in serum in measles infection.

We determined lactate dehydrogenase (LD; EC 1.1.1.27) in serum from 75 sequential measles patients and 105 control patients. In the measles patients, LD was increased to 1147.9 +/- 43.8 (mean +/- SE) U/L, markedly more than in the control group (517.2 +/- 15.0 U/L, p less than 0.001). Both LD-3 and LD-4 isoenzymes were increased, but the concentration of LD-5 was normal. Patients with high LD values (greater than 1500 U/L) had longer hospital stays than did those with lower values (p less than 0.01). Our results suggest that increased LD in serum is a common finding in measles infection and presumably originates from lymphocytes.

Bronchitis↗
PubMed1987

[Risk factors in the surgical repair of tetralogy of Fallot: analysis of preoperative factors by stepwise regression].

Fifty-one patients undergoing total repair of tetralogy of Fallot between November 1977 and June 1985 were analyzed with respect to determinants of operative mortality and postoperative right to left ventricular peak systolic pressure ratio (RV/LV). The risk factors were age, sex (Sex), previous shunting operation (Shunt), extent of right ventricular outflow tract patch (Patch), various PA-indexes, maximal left atrial volume/BSA (LA/BSA), left ventricular end diastolic volume index (LVEDVI), LVEDVI/normal-LVEDVI, RV/LV, Hb, BSA and weight. Univariate analysis indicated that RV/LV and LA/BSA were associated with operative death. A model for operative mortality with three variables emerged from the multiple stepwise regression analysis: Patch, Shunt and Sex (R = 0.421, R2 = 0.177, F = 3.37). A model for predicted RV/LV with five variables: Weight, Shunt, BSA, Patch and Sex (R = 0.602, R2 = 0.363, F = 5.12). However, it was thought difficult to predict operative mortality and postoperative RV/LV by the preoperative variables. Intra- and postoperative factors might have larger influences on operative mortality and RV/LV than the preoperative ones.

Analysis of Variance↗
PubMed1987

[Cytologic studies of cervical adenocarcinoma].

Seventy three cases of cervical adenocarcinoma, classified histologically according to WHO classification, were cytodiagnostic examined for cellular arrangement, cellular shape, nuclear shape, chromatin pattern and appearance rate of nucleolus. In adenosquamous carcinoma, the observed cancer cells were classified further into five categories and the incidence of each type was clarified. The authors obtained the following conclusions. The cytological findings in cellular arrangement, cellular shape, chromatin pattern and nucleolus findings were closely related with the degree of differentiation of endocervical type adenocarcinoma. Since no substantial cytodiagnostic differences were seen between endometrioid adenocarcinoma and endocervical type adenocarcinoma, it appears to be difficult to differentiate between them. Clear cell adenocarcinoma is characterized by sheet-like cell arrangement, polycrystal cell shape, round nuclei, finely granular chromatin pattern and appearance of macronucleolus. Among them the occurrence of macronucleolus was clearly observed and appeared of high value in making a diagnosis. Adenosquamous carcinoma, showing no characteristic finding in cytology, is proved to have morphological diversity. Careful observation of the intermediate type cancer cells is necessary to presume the histology of adenosquamous carcinoma.

Adenocarcinoma↗
PubMed1986

Molecular cloning and in vitro transcription of rat 4.5S RNAH genes.

4.5S RNAH (4.5S RNA associated with poly A containing RNA) has extensive homology to major interspersed repeat B1 in rodent genomes. We developed a new cloning technique for screening genomic library that eliminates the signal produced by repeated sequences or pseudogenes and applied it to cloning of 4.5S RNAH genes. Six phage clones (2, 3, 6, 9, 10 and 15) which hybridize with 4.5S RNAH were isolated from a rat gene library by this method. The restriction fragments containing the 4.5S RNAH locus were subcloned into plasmids and sequenced. Clones 2, 3, 9 and 15 contained one to five base substitutions in the coding region for 4.5S RNAH and were probably pseudogenes. In clone 2, the 4.5S RNAH locus was linked directly with the identifier sequence. Clone 6 contained three copies of the 4.5S RNAH gene (6a, b and c) which were clustered in the same direction within 455 base pairs. 6b was linked directly with 6c and ubiquitous repetitive DNA sequences B2 were inserted immediately after 6a and 6c. These three sequences as well as the sequence in clone 10 were colinear with rat 4.5S RNAH. In an in vitro transcription system, only clone 10 gave intact 4.5S RNAH.

Animals↗
PubMed1986

Cloning and characterization of rat 4.5S RNAI genes.

Genomic clones containing genes for 4.5S RNAI, which is an abundant small nuclear RNA found in rodent cells, were obtained from a rat genomic library. Thirty-four clones that formed RNase A resistant hybrids with 3'-end-labeled 4.5S RNAI were isolated, and seven of them (clones lambda I39, lambda I41, lambda I42, lambda I51, lambda I106, lambda I123 and lambda I154) were characterized by sequencing and in vitro transcription. Clones lambda I41 and lambda I123 carry one and two genes, respectively, with identical sequences to that of 4.5S RNAI and are actively transcribed in vitro. However, the other five clones contain sequences that seem to be pseudogenes for 4.5S RNAI, since they have nucleotide substitutions or deletions in the sequence corresponding to 4.5S RNAI or are not transcribed. Four clones (lambda I39, lambda I42, lambda I106 and lambda I154) were found to have 13-18 nucleotide-long direct repeats flanking the 4.5S RNAI sequences. The genomic organization of the genes and their related sequences is discussed.

Animals↗