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Biomedical subjects

Y Takeuchi

Publications and source records attributed to Y Takeuchi.

At least 847 records · Page 47Linked to original sources
PubMed1987

Influences of strain and diet on the promoting effects of sodium L-ascorbate in two-stage urinary bladder carcinogenesis in rats.

The influences of strain and diet on the promoting effects of sodium L-ascorbate (SA) on two-stage urinary bladder carcinogenesis was investigated in male F344 and Lewis rats. Two kinds of commercial basal diets, Oriental MF and Clea CA-1, were used. Rats were given 0.05% N-butyl-N-(4-hydroxybutyl)nitrosamine in their drinking water for 4 weeks and then basal diet with 5% SA or without SA for 32 weeks. Treatment with SA increased the induction of neoplastic lesions of the urinary bladder in rats initiated by 0.05% N-butyl-N-(4-hydroxybutyl)nitrosamine. The promoting effect of SA for urinary bladder carcinogenesis was: F344 strain-Oriental MF diet greater than Lewis strain-Clea CA-1 diet greater than F344 strain-Clea CA-1 diet = Lewis stain-Oriental MF diet. In both strains or with both diets, SA-treatment increased the urinary pH and the concentrations of sodium ion and total ascorbic acid. These results demonstrate that strain and diet strongly influence susceptibility to the SA-promoting effects in rat urinary bladder carcinogenesis.

Animals↗
PubMed1987

Postoperative deep body temperature rhythm.

The postoperative deep body temperature rhythms of fifteen patients who received aorto-coronary bypass surgery (group I), and of seven patients who received non-cardiac major surgery (group II), were studied. Postoperative patients, especially those who received aortocoronary bypass surgery, showed greatly disturbed deep body temperature rhythm. There existed infradian and ultradian rhythm in both groups, and there existed two patients in group I who did not show sinusoidal rhythm. The patients of group I also showed a longer period of rhythm than did those of group II. The mesor and amplitude of the patients in group I showed a greater individual variation than did those in group II. The acrophase of both groups deviated widely. The patients who underwent cardiac surgery needed a longer time for temperature rhythm recovery than did those who underwent general surgery.

Adult↗
PubMed1987

Molecular relationship between two types of phospholipase B from Penicillium notatum and reconstitution of active enzyme from its peptide fragments.

Two types of phospholipase B of Penicillium notatum, the native type and the modified type that is thought to be generated by the introduction of some nicks into the native type of enzyme by the endogenous protease(s), were distinguished on a slab sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis under a nonreducing condition. The native form migrated with a rate corresponding to 95K Da, whereas the modified form migrated more slowly, corresponding to 106K Da, presumably because of its more extended conformation. That the "106K" protein was indeed a nicked product of the "95K" protein was confirmed by amino acid analysis, peptide mapping, N- and C-terminal sequence analyses, and immunoblotting. The peptide fragments (70K and 37K + 32K) comprising the modified protein were isolated by gel filtration in the presence of SDS and 2-mercaptoethanol (the 32K peptide was suggested to be a partial proteolytic product of the 37K peptide). When the "95K" protein was subjected to the same treatment under denaturing condition, it retained a low, but significant, enzymatic activity; in contrast, the separated peptide fragments did not show any significant activity. By a coincubation of these fragments, however, a restoration of enzymatic activity was observed through a reformation of the active complex, corresponding to the original modified protein. The enzymatic activity of this complex was further increased by a treatment with guanidine X HCl, followed by dialysis. The association of peptide fragments appears to occur through the formation of interpeptidal disulfide bonds.

Amino Acid Sequence↗
PubMed1987

Direct connections of primary afferent fibers with spinal neurons projecting to the facial nucleus in the cat.

Spinal neurons projecting to the facial nucleus, located in the lateral portion of lamina V from the C1 to C4 segments, had long dendrites extending into lamina IV. The detailed morphology of these spinal neurons was confirmed using the Golgi impregnation method. Dorsal root fibers were distributed mainly to lamina IV at the C1 and C2 segments. These findings indicated the possibility of direct connections between the primary afferent fibers and spinal neurons projecting to the facial nucleus in lamina IV of the upper cervical cords. In order to confirm the connections, electron microscopic observation was carried out on the dorsal horn after injections of horseradish peroxidase into the facial nucleus and dorsal root fibers.

Afferent Pathways↗
PubMed1987

Treatment effect of combined maxillary protraction and chincap appliance in severe skeletal Class III cases.

The present study was designed to evaluate the true treatment effects of the maxillary protracting appliance with chincap for skeletal Class III cases and to evaluate the difference of true treatment effects between the cases in which the maxilla was protracted from the first molars and the cases protracted from the first premolars. Cephalograms of 63 cases (the first molar protraction group--27 cases protracted from the first molars, the first premolar protraction group--36 cases protracted from the first premolars) treated with the combined maxillary protraction and chincap appliance were used. Template analysis was performed to evaluate the estimated treatment effects without growth change. Forward movement of the maxilla and backward rotation of the mandible were characteristic features of the estimated treatment effects in 63 cases. In comparing the two groups, the maxilla was displaced more anteriorly and rotated more upward and forward in the first molar protraction group. Therefore, the intraoral site of protraction should be selected by considering vertical dimensions of skeletal and dental structures, and the amount of forward displacement of the maxilla required in the treatment of the individual patient.

Adolescent↗
PubMed1987

Synthesis and antitumor activity of tropolone derivatives. 5.

As part of a study on the structure-activity relationship of antitumor-active tropolone derivatives, a series of bistropone analogues, related to potently active bistropolone 1a, were synthesized and tested for their antitumor activity in in vitro (KB cell) and in vivo (leukemia P388 in mice) systems. The methoxytropones 3 and 5, hydroxytropothione 10, and (N-methylamino)tropones 12 and 13 were inactive in both systems. Methoxytropone 6 exhibited weak activity, whose potency was equal to that of monotropolone 2a.

Animals↗