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Biomedical subjects

Y Nomoto

Publications and source records attributed to Y Nomoto.

At least 163 records · Page 9Linked to original sources
PubMed1987

Detection of immunoglobulins and/or complement in kidney tissues from non-obese diabetic (NOD) mice.

Immunofluorescent studies were performed on kidney tissues from non-obese diabetic (NOD) mice. Tissues from twenty-two NOD mice ranging in age from 8 to 33 weeks were examined by immunofluorescence and light microscopy. It was shown that the immunofluorescent pattern of immunoglobulins and complement deposition in glomeruli from NOD mice were changed from diffuse linear, focal and/or local fine granular to diffuse coarse granular depending on the age. These findings in NOD mice were consistent with those in human diabetic nephropathy. It was suggested that the immunofluorescent studies in NOD mice are useful for evaluation of the pathogenesis of renal involvement in patients with diabetes mellitus.

Animals↗
PubMed1987

[Interventricular septal wall motion abnormality in left bundle branch block].

An experimental study was performed to clarify the mechanism of perfusion defects in the interventricular septum on T1-201 scintigraphy, as seen in patients with left bundle branch block (LBBB) having normal coronary arteries. In anesthetized open-chest dogs, the following parameters were assessed during right atrial pacing as a control, left ventricular pacing to produce right bundle branch block (RBBB), and right ventricular pacing for LBBB; 1. intramuscular pressure in the interventricular septum, 2. blood flow of the left anterior descending coronary artery (LAD) measured by an electromagnetic flowmeter; 3. regional myocardial blood flow (MBF) determined at three sites, including the interventricular septum, LAD area, and left circumflex coronary artery (LCx) area using the H2-washout method. Aortic pressure, left ventricular pressure, and M-mode echocardiograms were recorded during the procedures. During right ventricular pacing, LAD flow remained unchanged; whereas MBF at the interventricular septum decreased from 99.6 +/- 23.4 to 79.2 +/- 17.6 ml/min/100 g, but MBF at the LCx area increased from 103.2 +/- 19.8 to 122 +/- 18.4 ml/min/100 g. In contrast, there were no significant changes in regional flow in any sites during left ventricular pacing. During right ventricular pacing, an early systolic dip was observed in the septal wall concomitantly with the onset of rise in intramuscular pressure in the interventricular septum. However, the beginning of the rise in left ventricular pressure was delayed 33 +/- 4 msec after that of the septal intramuscular pressure.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1987

Clinical effect of newly developed oral carbonaceous adsorbent (AST-120) in patients with advanced stages of chronic glomerulonephritis.

Clinical effects of a newly developed oral carbonaceous adsorbent (AST-120) in patients with advanced stages of chronic glomerulonephritis is described. Measurements of serum creatinine were performed before, during and after the administration of AST-120. The reciprocal of serum creatinine concentration was also plotted serially in each patient. The mean levels of the slope of the reciprocal serum creatinine vs time plot after administration of AST-120 was decreased compared with those before the administration of AST-120 but there was no statistical significance (p greater than 0.10). Further studies with more patients are needed to determine whether the administration of AST-120 may be useful for treatment of patients with chronic glomerulonephritis.

Adsorption↗
PubMed1986

Effect of immunoglobulin deposition on the amount of glomerular sialic acids in patients with membranous nephropathy.

A study of double immunofluorescence staining of immunoglobulins and sialic acids in the glomeruli from patients with membranous nephropathy was described. Renal biopsy specimens from patients with membranous nephropathy were stained with rhodamine-labeled limulus polyphemus (LPA) and tricum vulgaris (WGA). The binding of LPA or WGA was hardly observed in the glomerular capillary walls from patients with membranous nephropathy although the deposition of immunoglobulin was marked. Both binding of LPA and deposition of IgG in the glomerular capillary walls were observed in only one out of nine patients with membranous nephropathy (11%). In that case, binding of LPA was observed in the inside of glomerular capillary walls although the deposition of IgG was observed on the outside of the walls. It was suggested that decrease of sialic acids in the glomerular capillary walls might be due to deposition of IgG in some patients with membranous nephropathy.

Arthropod Proteins↗
PubMed1986

Detection of immunoglobulins and other serum proteins in the dermal and glomerular capillary walls from patients with diabetes mellitus.

Deposition of immunoglobulins or acute phase reactant (APR) proteins in the dermal and glomerular capillary walls from patients with diabetes mellitus was examined to determine whether development of microangiopathy in such patients was related to exudation and/or entrapment of these proteins. Skin and renal biopsy specimens were obtained from patients with diabetic nephropathy and diabetes mellitus without nephropathy. These biopsy samples were stained with FITC-labeled anti-human IgG, IgA, IgM, C3, APR proteins, and beta-lipoprotein antisera. Linear depositions of IgA, IgG, and/or APR proteins were observed in the dermal and/or glomerular capillary walls from some patients with diabetic nephropathy or diabetes mellitus without nephropathy. It is indicated that deposition of such immunoglobulins in the dermal and glomerular capillary walls might be due to exudation and/or entrapment of these substances in patients with diabetes mellitus.

Adult↗
PubMed1986

Effect of immunoglobulin depositions of glomerular sialic acids in patients with IgA nephropathy.

A study of double immunofluorescence-staining of immunoglobulins and sialic acids in the glomeruli from patients with IgA nephropathy is described. Renal biopsy specimens from patients with IgA nephropathy were stained with rhodamine-labeled antihuman IgA, IgG or IgM antisera and then stained with FITC-labeled Limulus polyphemus (LPA), Tricum vulgaris (WGA) or antihuman C3 antisera. Marked positive stainings of IgA and C3 and positive binding of LPA or WGA were observed in the glomerular mesangial areas from patients with IgA nephropathy. LPA or WGA were not bound with glomerular capillary walls from patients with moderate and advanced stages of IgA nephropathy, although depositions of IgA and C3 were markedly observed in such walls. There was a significant inverse correlation between the deposition of IgA and the binding of LPA or WGA in glomerular capillary walls obtained from these patients with IgA nephropathy. The levels of proteinuria from patients with moderate and advanced stages of IgA nephropathy were significantly higher than those with minimal and slight stages of such disease. It is suggested that the decrease of sialic acids in glomerular capillary walls might be due to a deposition of IgA in some patients with IgA nephropathy. It is concluded that high levels of proteinuria might be due to the decrease of sialic acids in glomerular capillary walls from patients with moderate and advanced stages of IgA nephropathy.

Adult↗
PubMed1986

Cytopathic effects of antigens in patients with IgA nephropathy.

The cytopathic effects of extracts of pharyngeal cells from patients with IgA nephropathy on fibroblasts were determined. Autoradiographical analysis of antigens in the fibroblasts was also described. Freeze and thawed extracts of pharyngeal cells obtained from patients with IgA nephropathy, other glomerular diseases and healthy adults were cultured with fibroblasts such as Vero or Hel cells at 37 degrees C for 1 or 2 weeks. The cytopathic effects of fibroblasts were examined with a light microscope. In addition, the eluate obtained from renal tissues of IgA nephropathy was labeled with iodine-125 by the chloramine-T method. These cultured fibroblasts were incubated with iodine-125-labeled eluate from the same or other patients with IgA nephropathy. The degree of cytopathic effects of extracts of pharyngeal cells from patients with IgA nephropathy on Vero or Hel cells was significantly increased compared with that from patients with other glomerular diseases or healthy adults without glomerular diseases. The cytopathic effects of such fibroblasts were not inhibited by filtration of extracts of pharyngeal cells obtained from patients with IgA nephropathy. It was shown that the antibodies eluted from renal tissues of patients with IgA nephropathy specifically bound with the nuclear regions of such fibroblasts. It was suggested that some antigenic substances might exist in the epithelial cells of the upper respiratory tracts of some patients with IgA nephropathy, and that such antigens were able to be transferred to cultured fibroblasts.

Adult↗
PubMed1986

Successful use of continuous ambulatory peritoneal dialysis (CAPD) in the treatment of patients with renal and cardiac failure.

This report described successful uses of continuous ambulatory peritoneal dialysis (CAPD) in the treatment of two patients with renal and cardiac failure. Case 1 was a 46-year-old woman with renal failure and mitral regurgitation. There were some predictable disadvantages to make a blood access and to increase the cardiovascular stress for her condition. Therefore, we treated her using CAPD and succeeded in improving her condition. Case 2. was a 62-year-old man with renal failure and aortic insufficiency. Beside having renal failure, he showed intractable cardiac failure. He was initially treated using in full ECUM (extracorporeal ultrafiltration method) and CAPD. Two week after admission he was successfully treated only by CAPD. It is suggested that CAPD will be the first choice of treatment in patients with renal and cardiac failure.

Aortic Valve Insufficiency↗
PubMed1986

Clinical effect of dipyridamole in patients with IgA nephropathy.

Clinical effects of dipyridamole and carbazochrome sodium sulfonate in patients with IgA nephropathy are described. Oral administration of 300 mg of dipyridamole and 180 mg of carbazochrome sodium sulfonate per day was employed in the present study. Urinalysis and renal function tests, i.e. serum creatinine (s-Cr), blood urea nitrogen (BUN), glomerular filtration rate (GFR) and phenolsulfonphtalein (PSP) tests, were performed before and after the administration of dipyridamole. It was demonstrated that the administration of dipyridamole was effective in reducing the level of proteinuria in the patients. The administration of carbazochrome sodium sulfonate was not effective in reducing the proteinuria level. It was concluded that the administration of dipyridamole may be useful for treatment of patients with IgA nephropathy.

Adrenochrome↗
PubMed1986

Detection of alpha 2-pregnancy associated-glycoprotein (alpha 2-PAG) in cultured human peripheral blood mononuclear cells.

A study of the detection of alpha 2-pregnancy associated-glycoprotein (alpha 2-PAG) by double immunofluorescence in cultured human peripheral blood mononuclear cells (MNC) was performed. MNC was obtained from four healthy adults and isolated on a Ficoll-Hypaque density gradient. MNC was incubated with RPMI-1640 with or without pokeweed mitogen in 5% CO2/air at 37 degrees C for six days. MNC was initially stained with rhodamine-conjugated antihuman IgA, IgM, and IgG antisera, and then stained with FITC-conjugated antihuman alpha 2-PAG antisera. It was concluded that alpha 2-PAG was not produced by IgA, or by IgM and IgG producing cells in healthy adults.

Cells, Cultured↗
PubMed1986

Siblings with IgA nephropathy and diffuse proliferative glomerulonephritis (PGN) associated with identical HL-A antigens.

Siblings with IgA nephropathy and diffuse proliferative glomerulonephritis without mesangial IgA deposits (PGN) who had identical HL-A antigens are described. A 21-year-old woman suffered from IgA nephropathy and her 27-year-old sister showed diffuse proliferative glomerulonephritis (PGN) distinct from IgA nephropathy. These siblings had identical HL-A alloantigens which were A2, Aw24; Bw16, Bw35; Cw4, Cw7; DR4. Microhematuria and increased serum IgA levels were shown in their father and other sister but renal biopsies were not performed. The serotypes for the HL-A antigens of their father and other sister were A2, Aw24; Bw27, Bw35; Cw4; DR4 and A2; Bw16; Cw7; Dr2, respectively. It is demonstrated that IgA nephropathy and PGN share a common HL-A phenotype including DR4. It is postulated that the presence of DR4 antigens is not directly related to the occurrence of IgA nephropathy.

Adult↗
PubMed1986

Characterization of an acidic nuclear protein recognized by autoantibodies in sera from patients with IgA nephropathy.

A nuclear antigen is recognized by autoantibodies in the sera of some patients with IgA nephropathy. Using these autoantibodies as a reagent, this antigen was purified 77.2-fold by ammonium sulfate fractionation, DEAE chromatography and Sepharose 6BCL gel filtration. The antigenicity of this antigen was sensitive to trypsin but resistant to RNase and DNase, suggesting that the antigenic determinant resided in protein and not nucleic acids. This antigen was inactivated at 56 degrees C for 3 h. Isoelectrophoretic focussing showed that the pI was below 4. The immunoblotting (Western transfer) assay showed a single polypeptide (69,000 Daltons) which proved to be a reactive antigen.

Antibodies, Antinuclear↗
PubMed1986

Significant correlation between the immunofluorescence of alpha 2-plasmin inhibitor in glomeruli and the effects of urokinase therapy in patients with IgA nephropathy.

Correlation between the deposition of alpha 2-plasmin inhibitor (alpha 2-PI), which is one of the inhibitory factors of fibrinolytic activities, in the glomeruli and the effects of urokinase therapy in patients with IgA nephropathy is described. Urokinase (UK) is a plasminogen activator derived from fresh human urine. Urinalysis and measurements of renal function tests, i.e., serum creatinine, blood urea nitrogen, glomerular filtration rate and phenolsulfonphtalein, were performed before and at 8 and 48 weeks after the administration of urokinase. There was marked improvement of proteinuria after UK therapy in patients without deposition of alpha 2-PI in the glomeruli. In contrast, the improvement of proteinuria after UK therapy was not observed in patients with positive deposition of alpha 2-PI in the glomeruli. It was concluded that the administration of UK may be useful for treatment of proteinuria in patients with IgA nephropathy.

Fluorescent Antibody Technique↗
PubMed1986

An adult case of Fanconi's syndrome associated with membranous nephropathy.

An adult case of Fanconi's syndrome associated with membranous nephropathy is described. Renal biopsy specimens revealed typical features of membranous nephropathy associated with severe tubulo-interstitial changes in light microscopy, electron microscopy and immunofluorescent microscopy. The nephrotic syndrome due to membranous nephropathy was observed at the time of definite diagnosis of Fanconi's syndrome in this patient. It is suggested that renal tubular injuries in some patients with membranous nephropathy might be an important factor in the pathogenesis and/or development of Fanconi's syndrome.

Fanconi Syndrome↗