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Biomedical subjects

Y Murakami

Publications and source records attributed to Y Murakami.

At least 973 records · Page 54Linked to original sources
PubMed1982

Purification and some properties of ornithine decarboxylase from rat liver.

Ornithine decarboxylase (EC 4.1.1.17) was purified to near homogeneity from the livers of thioacetamide- and DL-alpha-hydrazino-delta aminovaleric acid-treated rats by using three types of affinity chromatography with pyridoxamine phosphate-Sepharose, pyridoxamine phosphate-dipropylenetriamine-Sepharose and heparin-Sepharose. This procedure gave a purification of about 3.5.10(5)-fold with an 8% yield; the specific activity of the final enzyme preparation was 1.1.10(6) nmol CO2/h per mg protein. The purified enzyme gave a single band of protein which coincided with activity peak on polyacrylamide gel electrophoresis and also gave a single major band on SDS-polyacrylamide gel electrophoresis. A single precipitin line was formed between the purified enzyme and an antiserum raised against a partially purified enzyme, on Ouchterlony immunodiffusion. The molecular weight of the enzyme was estimated to be 105000 by polyacrylamide gel electrophoresis at several different gel concentrations; the dissociated subunits had molecular weights of 50000 on SDS-polyacrylamide gels. The isoelectric point of the enzyme was pH 4.1.

Animals↗
PubMed1982

A macromolecular inhibitor of the antizyme to ornithine decarboxylase.

A macromolecular factor that inhibits the activity of the antizyme to ornithine decarboxylase (ODC) was found in rat liver extracts. The factor, 'antizyme inhibitor', was heat-labile, non diffusable and of similar molecular size to ODC. The antizyme inhibitor re-activated ODC that had been inactivated by antizyme, apparently by replacing ODC in a complex with antizyme. Therefore the antizyme inhibitor can be used to assay the amount of inactive ODC-antizyme complex formed in vitro. When assayed by this method, the complex was shown to be eluted before ODC from a Sephadex G-100 column. Significant increase in ODC activity was observed when the antizyme inhibitor was added to crude liver extracts from rats that had been injected with 1,3-diaminopropane to cause decay of ODC activity, suggesting the presence of inactive ODC-antizyme complex in the extracts.

Animals↗
PubMed1982

Esophageal reconstruction with a skin-grafted pectoralis major muscle flap.

Reconstruction of the cervical esophagus is a problem in the surgical treatment of hypopharyngeal cancer. The conventional deltopectoral skin flap and the pectoralis major myocutaneous flap both have shortcomings. We developed a new technique for the immediate reconstruction of the entire cervical esophagus that utilizes the pectoralis major muscle with skin grafted directly onto it. This is especially useful in female patients who are buxom or have thick subcutaneous adipose tissue, because the anterior chest skin is not used as the skin island and, therefore, the flap is not too bulky and cosmetic problems are minimal. The breast is left unaffected with the nipple in its normal position. We report the technical details and the results in four female patients with hypopharyngeal cancer.

Esophagus↗
PubMed1982

Radiation therapy of early glottic carcinoma. A Japanese experience.

From 1969 and through 1979, 105 patients with T1 N0M0 and T2 N0M0 glottic carcinoma were treated with irradiation alone. The minimum follow-up period was 2 years, and only one patient was lost to follow-up. There were 62 T1a lesions, 14 T1b, 19 T2 with normal mobility, and 10 T2 with impaired mobility; relapses occurred in 10, 4, 1 and 3 patients, respectively. All but one patient who relapsed had local recurrence with or without nodal or distant spread. One patient had only nodal metastasis. Six of the 18 relapses were successfully cured by surgery. None of the patients who had nodal or distant spread survived. The relapse rates had no clear relation to total dose or TDF value. T1 lesions treated through larger fields (greater than or equal to 25 cm2) had a lower relapse rate than lesions treated through smaller fields, but the difference was not statistically significant.

Adult↗
PubMed1982

Experimental studies on vertical infection of mice with Japanese encephalitis virus III. Effect of gestation days at the time of inoculation on placental and fetal infection.

An experiment was carried out on the effect of gestation days at the time of inoculation on the establishment of experimental vertical infection with Japanese encephalitis virus in mice. In it, mice of the CFW strain in a closed colony were inoculated intravenously with a field strain at different times over a period from 3 to 12 days of gestation. After that, an attempt was made to recover the virus from the placenta and fetus to estimate the rate of infection. As a result, placental infection was established not when the virus was inoculated at 3 days of gestation, but when it was inoculated at 4 days of gestation or later. The rate of infection was relatively high when the virus was inoculated at 6 days of gestation or later. Fetal infection was established relatively frequently when the virus was inoculated some time between 7 and 10 days of gestation, but quite infrequently when the virus was inoculated at any other time than this. There was a difference in rate between placental and fetal infection at a given time of inoculation in days of gestation. This difference seemed to have been induced not by a difference in intensity of viremia appearing after inoculation, but by a difference in degree of development between placental and fetal tissues at the time of inoculation.

Animals↗
PubMed1982

Establishment of an attenuated strain of porcine parvovirus by serial passage at low temperature.

To prepare a live virus vaccine strain for the prevention of porcine parvovirus infection, the 90HS strain, isolated from the brain of a stillborn porcine fetus, was subjected to the first 45 serial passages in swine kidney established (ESK) cells of porcine kidney origin at 30-35 degrees C and to the 46th and later serial passages in the same cells as these at 32 degrees C. When swine were inoculated with the strain at the 38th passage level possessing such properties as expressed with rct/37+ and rct/40-, they presented viremia, virus discharge, and the transmission of virus to other swine. When swine were inoculated with the strain at the 54th and 55th passage level possessing such properties as expressed with rct/37- and rct/40-, they failed to exhibit viremia, virus discharge, and the transmission of virus to other swine, but retained for a long time hemagglutination-inhibiting antibody which had been produced after inoculation. A low virulent variant strain was obtained after 54 serial passages at low temperature. It was called the HT- strain.

Animals↗
PubMed1982

Immunity to infection with porcine parvovirus in pigs inoculated with the attenuated HT- strain.

Swine were inoculated once with the attenuated HT- strain of porcine parvovirus. Several months later they were challenged by oral inoculation with a field strain of the virus to examine an ability to prevent infection. After challenge inoculation unimmunized control swine exhibited an increase in antibody titer, viremia, and virus discharge. The virus was recovered from many organs. The swine preinoculated with the attenuated HT- strain, however, manifested none of these symptoms and were negative for virus recovery from any organ.

Animals↗