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Biomedical subjects

Y Mizoguchi

Publications and source records attributed to Y Mizoguchi.

At least 199 records · Page 11Linked to original sources
PubMed1986

Detection of the cholestatic factor in the liver tissue of patients with acute intrahepatic cholestasis.

A novel lymphokine, which we have designated as the cholestatic factor (CF). was produced from peripheral blood lymphocytes of patients with drug-induced allergic intrahepatic cholestasis by stimulation with a causative drug in the presence of the liver soluble fraction containing liver-specific lipoprotein (LSP). Marked reductions in bile flow and bile acid excretion were induced in rats by injecting CF through a mesenteric vein. In order to confirm the presence of CF in the liver tissue of patients, we attempted to detect this lymphokine by using the immunofluorescence technique. As a result, CF was found in the liver tissue of six out of 30 patients with acute intrahepatic cholestasis including one with hepatitis A type, one with hepatitis B type, one with hepatitis non-A non-B type and three with drug-induced allergic hepatitis. In contrast, CF was undetectable in the liver tissue of patients without intrahepatic cholestasis. These results may additionally support our assumption that CF plays an important role in the induction of intrahepatic cholestasis in various liver diseases.

Adult↗
PubMed1985

Studies on the effects of estrogen on in vitro antibody production in autoimmune liver diseases, including lupoid hepatitis and primary biliary cirrhosis.

Antibody-forming cells produced by adding trinitrophenylated sheep red blood cells (TNP-SRBC) were induced, when peripheral blood mononuclear cells from normal individuals and patients with autoimmune liver diseases, including lupoid hepatitis and primary biliary cirrhosis, were stimulated in vitro with pokeweed mitogen (PWM). Although antibody responses were significantly augmented by adding estrogen simultaneously with PWM to mononuclear cell cultures prepared from normal individuals and autoimmune liver diseases patients, a significant difference was observed according to the concentrations of estrogen between the normal subjects and patients. These observations suggest that a different responsiveness to the different concentrations of estrogen underlines the immunological abnormalities involved in autoimmune liver diseases, including lupoid hepatitis and primary biliary cirrhosis.

Adult↗
PubMed1985

Studies on the effects of estrogen on antibody responses in asymptomatic HB virus carriers and non-responders to HB vaccine inoculation.

Antibody-forming cells against trinitrophenylated sheep red blood cells (TNP-SRBC) were induced to similar extents, when peripheral blood mononuclear cells from normal individuals, non-responders to HB vaccine inoculation and asymptomatic HBV carriers were stimulated in vitro with pokeweed mitogen (PWM). Although these antibody responses were significantly augmented by adding estrogen simultaneously with PWM to mononuclear cell cultures prepared from normal individuals, no such augmentation was demonstrable in cultures from asymptomatic HBV carriers and non-responders to HB vaccine inoculation. Interleukin-1 production and DNA synthesis in PWM- and PHA-stimulated mononuclear cell were also increased by estrogen in normal individuals, but not in the other two groups. These observations suggest that asymptomatic HBV carriers and non-responders to HB vaccine inoculation share a similar deficiency, not found in normal subjects, with regard to the modulatory effects of estrogen.

Antibody Formation↗
PubMed1985

Protection of liver cells from experimentally induced liver cell injury by glycyrrhizin.

Liver cell damage is induced when isolated liver cells coated with specific antibody against the liver cell membrane are cultured with peripheral blood mononuclear cells. Although this antibody-dependent cell-mediated cytotoxicity (ADCC) was induced by closed contact of effector cells with targets via specific antibody, a cytotoxic factor or factors causing inhibition of protein synthesis in liver cells was detected in the culture supernatant of the ADCC reaction. Similarly, peritoneal exudate macrophages activated by endotoxin lipopolysaccharide (LPS) also had cytotoxic effects on isolated liver cells by producing a cytotoxic substance or substances. These liver cell injuries caused by either ADCC or activated macrophage culture supernatants were significantly reduced by pretreatment of the isolated liver cells with glycyrrhizin before the addition of the cytotoxic culture supernatants. These results suggest that glycyrrhizin may protect liver cells from immunological injuries.

Animals↗
PubMed1985

Direct estimation of cardiac reserve through analysis of relation between oxygen consumption and heart rate during exercise testing.

In order to elucidate fundamental relationship between exercise and cardiac reserve, we investigated the parameters of regression equation (VO2 = A X HR + B) between oxygen consumption (VO2 ml/kg/min) and heart rate (HR) during exercise test (Bruce). 62 male children were divided into 3 groups based on history and clinical evaluation. HR related linearly with VO2 (average r = 0.967, range 0.90-0.993). Both of the parameters A and [B] were significantly high in Athlete (A = 0.465 +/- 0.043, [B] = 36 +/- 6.07), low in Failed (0.297 +/- 0.055, 17.4 +/- 6.1) and middle in Ordinary group (0.366 +/- 0.078, 23.5 +/- 8.6) (p less than 0.005). Based on our result of linear VO2-HR relation, and on the previously reported studies that cardiac output linearly and consistently relates with VO2, we considered the meaning of the parameters as follows; A determines the possible maximum stroke volume (SV), and B determines the HR where SV reaches plateau. In conclusion, since the SV is considered as a measure of cardiac function, measuring the parameters of VO2-HR relation allows us to isolate cardiac functional reserve through the exercise tolerance test.

Adolescent↗
PubMed1985

Left ventricular diastolic pressure-volume and stress-strain relationship in children.

Diastolic pressure and volume (P-V) curves were approximately exponential and fitted the equation, dP/dV = aP + b, where a was left ventricular volume elastic constant. Stress and strain (sigma-epsilon) curves were expressed by the equation, d sigma/d epsilon = k sigma + c, where k was wall stiffness constant. These exponential curves have been fitted over the whole diastole, but theoretically, the mid-diastole should reflect diastolic elastic properties best. In the present study, therefore, special attention was paid to the mid-diastole in each patient, and both P-V and sigma-epsilon relationships were analyzed by fitting the data to the above mentioned curves during this period of time. This analysis was made in two separate groups of patients. One was the control group consisting of 2 patients with normal hearts, 2 patients with mild pulmonary stenosis, and 25 patients with post mucocutaneous lymph node syndrome. The other group consisted of patients with postoperative congenital heart disease, that is, 8 patients with atrial septal defect and 5 patients with tetralogy of Fallot. The elastic constant (a) could not be compared if the size of the hearts differed. The results of the present study, however, demonstrated that the size of the heart was closely related to the constant, a. In the control group, the constant (a) was exponentially related to the size of the heart, and expressed as follows: a = 0.30e-0.037EDV + 0.045 (r = 0.94, p less than 0.01) In contrast, the wall stiffness constant (k) was not related to the size of the heart. After surgical repair of congenital heart disease, the stiffness constant in the left ventricle was normal in patients with postoperative atrial septal defect, while it was significantly increased in patients with postoperative tetralogy of Fallot.

Cardiac Volume↗