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Biomedical subjects

Y Mizoguchi

Publications and source records attributed to Y Mizoguchi.

At least 217 records · Page 12Linked to original sources
PubMed1985

Possible participation of cholestatic factor in the pathogenesis of intrahepatic cholestasis in alcoholic liver disease.

To elucidate the immunopathogenesis of intrahepatic cholestasis in alcoholic liver disease, the authors investigated the possible participation of a cholestatic factor. The peripheral lymphocytes from patients with intrahepatic cholestasis in alcoholic liver disease were stimulated with ethanol and liver specific lipoprotein in vitro and the culture supernatant was fractionated by gel filtration, using a Sephadex G-75 column. When a definitive fraction (fraction 4) was injected into the mesenteric vein of rats, a marked reduction in bile flow was observed. Similar results were obtained when the blood serum of patients was fractionated in an identical manner and the same fraction was injected into rats via the mesenteric vein. Histologically, dilated bile canaliculi with diminution of microvilli and increased vesicles around the dilated canaliculi were observed by electron microscopy after injection of the active fraction into rats. These results strongly suggest that not only the sensitized lymphocytes produce the cholestatic factor which caused the intrahepatic cholestasis by specific stimulation, but also this factor is involved significantly in the pathogenesis of intrahepatic cholestasis which is observed in patients with intrahepatic cholestasis in alcoholic liver disease.

Animals↗
PubMed1985

Changes in action potential and beta-adrenergic effects of the circular muscle of postpartum rat uterus.

Spike potential dominated in the circular muscle of postpartum rat uterus during the period between 0 and 15 hr after the delivery of the first newborn. During the postpartum period ranging between 20 and 48 hr, the plateau potential was dominant. Application of 10(-9) M isoprenaline strongly depressed the contraction during early postpartum period (0-15 hr), and the depressant effect was much smaller thereafter. In vivo treatment of postparturient rats with estradiol-17 beta (50 micrograms) or progesterone (50 micrograms) for 2 days did not alter the postpartum change in action potential or the effect of isoprenaline. The postpartum changes in muscle properties mentioned above were prevented in the distended portion of uterus, when several fetuses and placentas were artificially kept inside the uterus for 2 days, while other fetuses were delivered out. The hormonal influences on the circular muscle of postpartum rat uterus were discussed in view of the above experimental findings.

Action Potentials↗
PubMed1985

Effects of polyamines on the mechanical and electrical activities of the isolated circular muscle of rat uterus.

Effects of polyamines (0.05-1.2 mM) on the mechanical and electrical activities in the circular muscles of pre-(day 20 of pregnancy) and post-partum (10-15 h after delivery) rat myometria were studied. In the prepartum preparations, spermine and spermidine, added to the Mg-free Krebs solution, depressed contractions in a dose-dependent manner, whereas cadaverine and putrescine potentiated them. The latter agents depressed contractions when preparations were treated with indomethacin. Plateau potentials were spontaneously generated in the Mg-free solution in the prepartum circular muscle. The duration of the plateau became shorter, and spike potentials supermounted on the plateau potential were depressed when spermine or spermidine was applied. In the postpartum preparations, spike activity was depressed by spermine and spermidine. In both pre- and post-partum preparations, the membrane activity was little affected by cadaverine and putrescine. In view of the above findings, it was hypothesized that polyamines in general took the place of divalent cations in causing membrane stabilization. Cadaverine and putrescine probably caused a potentiation of contraction by stimulating the synthesis of endogenous prostaglandins.

Animals↗
PubMed1985

Studies on the effects of estrogen on the antibody response in asymptomatic HB virus carrier.

Antibody-forming cells against trinitrophenylated sheep red blood cells (TNP-SRBC) were induced to a similar extent when peripheral blood mononuclear cells from normal individuals, patients with chronic active hepatitis, and asymptomatic HBV carriers were stimulated in vitro with pokeweed mitogen (PWM). Although these antibody responses were significantly augmented by adding estrogen simultaneously with PWM to mononuclear cell cultures prepared from normal individuals and patients with chronic active hepatitis, no such augmentation was demonstrable in asymptomatic carriers. The DNA synthesis in PWM-stimulated mononuclear cells was also increased by estrogen in normal individuals and patients with chronic active hepatitis, but this was not the case in asymptomatic carriers. These observations suggest that a factor (or factors) may be correlated at least partially with the induction of the asymptomatic carrier state, such as unresponsiveness to the estrogen.

Antibody Formation↗
PubMed1984

Adult-type systemic varicella in a female patient in course of treatment of her so-called reactive lymphocytic (T-cell) hyperplasia.

A 31-year-old woman was examined in this case. Biopsy revealed an abnormal nontumorous proliferation of T-cells in the lymph node. Clinically, the patient was treated by C-MOPP and steroid under clinical suspicion of T-cell lymphoma. In the period of observation of the course of illness, the patient contracted varicella from her real child and died on the 8th day of illness. She was examined pathologically, electron microscopically, and by the fluorescent antibody technique. Virus infections foci, which were mostly necrotic ones, were found in the parenchymatous and interstitial cells of skin, lung, adrenal gland, digestive organs, liver, lymph nodes, and genital organs. In the circulatory system, proliferative foci with degeneration of endothelial and adventitial cells were noticed in small veins or venules, but little change was found in the arterial system. Electron microscopic observation suggested a destruction of the so-called limiting basement membrane around blood vessels. This was an important finding indicative of a mechanism of viral transmission to the interstitial cell system.

Adult↗
PubMed1984

Physiological significance of the slope of the regression equation between oxygen consumption and heart rate in exercise testing.

The relationship between oxygen consumption (VO2) and heart rate (HR) was studied in 62 male children. Based on clinical evaluation and history, they were divided into three groups, i.e., athlete, ordinary and failed. There was a high linear correlation between HR and VO2 in each individual. The averaged values of the slope and standard deviations were 2.09 +/- 0.189 in the athlete group (n = 17), 2.60 +/- 0.140 in the ordinary group (n = 16) and 3.17 +/- 0.591 in the failed group (n = 29). The statistical differences were confirmed among the groups (p less than 0.005). The results suggested that the slope of the HR vs. VO2 relation was related to an inotropic state of cardiac function. We concluded that the slope was a more suitable and more direct evaluation of cardiac function during exercise. Moreover, the method was non-invasive and safe because it required no potentially hazardous maximum work load for the patients.

Child↗