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Biomedical subjects

Y Kondo

Publications and source records attributed to Y Kondo.

At least 811 records · Page 45Linked to original sources
PubMed1986

[Effect of sulfamonomethoxine and ormetoprim on leucocytozoon infection in chickens].

The preventive effect of sulfamonomethoxine (Smm) and ormetoprim (Omp) medicated in combination against leucocytozoon infection in chickens was tested in field trials. In trial 1, medication was given continuously throughout the experimental period. In trial 2, medication was given for seven days and no medication was given for the next seven days; then this schedule for the medication was repeated throughout the experimental period. In trial 1, almost complete prevention was obtained when Smm and Omp were added to the feed at the level of 12 ppm and 14 ppm, respectively, or at the level of 15 ppm and 5 ppm, respectively. Only a partial effect was obtained, however, when these drugs were used at the level of 9 ppm and 3 ppm, respectively. In trial 2, a nearly complete effect was obtained when Smm and Omp were added to the feed at the level of 18 ppm and 6 ppm respectively, but we found no effect when these drugs were used at the level of 15 ppm and 5 ppm, respectively. There were no significant differences among the groups as to the weight gain.

Animals↗
PubMed1986

[Randomized cross-over study on the effects of methylprednisolone, metoclopramide and droperidol on the control of nausea and vomiting associated with cis-platinum chemotherapy].

A randomized cross-over study on the effects of methylprednisolone (MP), metoclopramide (MT) and droperidol (DP) on the control of nausea and vomiting associated with cis-platinum chemotherapy was performed. Sixty-three patients were entered into the study; 60 patients (21 treated with MP, 18 with MT and 21 with DP) were eligible for evaluation of antiemetic efficacy, whereas the side effects of these drugs were evaluated in all of the 63 patients. Out of the above 60 patients, 36 were entered into the cross-over treatment. Antiemetic efficacy was evaluated in terms of duration of nausea, duration of vomiting, frequency of vomiting, duration of inability to take food and the amount of food first taken after cis-platinum treatment. Generally, the MP-treated group of patients showed favorable effects in these points, MP being especially more effective than other drugs with a statistically significant difference for the control of nausea and disturbed intake of food during the first 24 hours after the treatment with CDDP. Anticipatory nausea and vomiting were not observed in this study. Side-effects were minimal and all of the patients except one, who showed severe diarrhea following administration of MT and could not tolerate the treatment thereafter, were safely treated.

Adult↗
PubMed1986

[Intraperitoneal cavity injection of OK-432 with fresh human serum in a case of carcinomatous peritonitis--determination on the complement-derived chemotactic factor].

Large numbers of neutrophils appear in the peritoneal cavity within a few hours after administration of OK-432 into the peritoneal cavity, and play an important role in the destruction of cancer cells. As OK-432 can activate complement in vitro, it is possible that a chemotactic factor such as C5a is generated and attracts neutrophils into the cavity. We injected OK-432 with fresh human serum preincubated at 37 degrees C for 60 min in order to generate large amounts of complement-derived chemotactic factor. Neutrophils increased in number within a few hours after the injection of OK-432 with preincubated human serum into the peritoneal cavity of a recurrent gastric cancer patient with carcinomatous ascites, and persisted much longer compared with treatment using OK-432 alone. Cancer cells rapidly decreased in number and ascites disappeared two weeks after the treatment with OK-432 and preincubated human serum. It was found that C5a levels measured by RIA initially increased a few hours before neutrophils appeared, and then decreased gradually, after injection of either OK-432 with preincubated human serum or with OK-432 alone. Although complement-derived chemotactic factor might be responsible for attracting neutrophils into the peritoneal cavity, the role of other chemotactic factors should also be investigated.

Adenocarcinoma↗
PubMed1985

Ultrastructural organization of the glomerular basement membrane as revealed by a deep-etch replica method.

The fine structure of the glomerular basement membrane was re-evaluated by using a deep-etch replica method. The structure of the laminae rarae interna and externa of the rat glomerular basement membrane was basically identical in that 6 to 8 nm fibrils were interconnected to form a three-dimensional, polygonal network. The size of the mesh was quite variable but most often ranged from 20 to 25 nm in width. In addition, a zipper-like substructure of the epithelial slit diaphragm was observed. By contrast, the lamina densa was composed of closely packed particles. After exposure of the bovine glomerular basement membrane to ultrasonic waves or trypsin, the particles of the lamina densa were effectively removed. The underlying structure showed the fibrillar network closely resembled that seen in the laminae rarae of the rat glomerular basement membrane. The glomerular basement membrane thus revealed was as principally composed of a fibrillar network, which might be regularly arranged units of type-IV collagen. Numerous fine particles, most likely proper components of the glomerular basement membrane, were attached onto this basic fibrillar structure, giving rise to a morphologic appearance different from that of the laminae rarae.

Animals↗
PubMed1985

Epithelioid haemangioendothelioma of the bone tissue.

An epithelioid haemangioendothelioma arising in a 43-year-old Japanese woman is presented. The patient first complained of severe back pain and neurogenic disturbances due to compression fracture of the spinal bone. Radiographically the tumour masses showed osteolytic lesions without reactive new bone formation. Similar lesions were noted in other bones. Biopsy and surgical specimens showed the features of epithelioid haemangioendothelioma. A lymphocyte associated antigen, HLA-DR which has been regarded as a B lymphocyte marker was detected in the tumour cells. The importance of this finding was emphasized with respect to the identification or classification of vascular tumours.

Adult↗
PubMed1985

Electron microscopy of monoclonal antibody-linked chains of thyroglobulin molecules.

Negative staining electron microscopy visualized immune complexes of hog thyroglobulin produced by 3 monoclonal antibodies, each towards a different conformation-dependent antigenic structure of the thyroglobulin. Equimolar mixtures of the thyroglobulin and any one of the 3 antibodies formed peculiar unbranched chains of 'ovoid' thyroglobulin molecules linked by the antibody molecules in an 'end to end' fashion. A mixture of the thyroglobulin and the 2 different antibodies produced branched chains. A set of 3 conformation-dependent antigenic structures was thus located near each end of the elongated thyroglobulin molecule. The results also indicated a topological similarity between the 2 subunits of thyroglobulin.

Animals↗
PubMed1985

Organ specificity of epithelial cells grown in tissue culture from explants obtained from various levels of the rat gut.

Monolayer cultures of epithelial cells grown from explants of fetal rat small intestine can differentiate into columnar as well as goblet cells in tissue culture (Kondo et al., Exp cell res 153 (1984) 12) [9]. In this study we have cultured tissue from various levels of the fetal rat gastrointestinal tract in order to characterize those cell types that can be cultured from these tissues and to determine the growth potential of these cells using this culture system. Explants of esophagus and forestomach tissue yielded monolayer outgrowths of squamous epithelial cells which grew as closely apposed polygonal cells capable of developing cornified envelopes. Explants of the glandular portion of the stomach yielded outgrowths of densely packed cells which were more pleiomorphic and which did not desquamate or form cornified envelopes. Explants of small intestine and colon yielded outgrowths of epithelial cells, some of which differentiated into goblet cells, while others developed a 'columnar' cell morphology. The following differences between squamous- and non-squamous cultures were observed. Squamous epithelium showed self-sustained growth, while growth of non-squamous epithelial cells became self-limiting. Cytochalasin B (CB) (5 micrograms/ml for 1 h) induced contraction of the whole cell sheet of non-squamous cells, but of only individual squamous cells. Squamous cell outgrowth was observed from tissues derived from fetuses of all ages (13-day fetuses through to 3-week-old rats); whereas non-squamous epithelial outgrowth was poor when rats older than the 21st gestational day were studied. These results indicate that cultures established in this manner developed the general characteristics of the cells of the organ from which they were derived, even when undifferentiated fetal tissue was used. The growth conditions needed for columnar epithelium are more stringent than those needed for squamous tissues. This technique opens the way for further characterization of growth requirements of gastrointestinal columnar epithelium.

Animals↗
PubMed1985

Daunomycin-induced nephropathy in rats.

A single intravenous injection of daunomycin into rats induced severe glomerular injury with massive proteinuria. Mesangial thickening due to an increase in the matrix appeared as early as 5 weeks after injection. Focal and segmental glomerular tuft distortion developed by 10 weeks associated with a progressive mesangial change, which was accompanied by detachments of endothelial cells and podocytes from the glomerular basement membrane (GBM) resulting in obliteration of the affected tufts. After 20 weeks, the lesion ultimately progressed to cause diffuse and global glomerular obliteration. Scattered glomeruli also showed frank shrinkage with a mild obliterative change. By observing a number of isolated glomeruli in scanning electron microscopy, it was revealed that podocyte alterations were variable from case to case and foot processes remained discrete in some cases until 10 weeks, despite the presence of marked proteinuria. Anionic sites distributed throughout the GBM and on the surface of podocytes were usually preserved in proteinuric rats as far as evaluated by ruthenium red and colloidal iron stainings. Our results indicate that loss of foot processes and of glomerular anionic sites are not causative factors but consequences of proteinuria.

Animals↗
PubMed1985

Lipaemia retinalis in a 29-day-old infant with type 1 hyperlipoproteinaemia.

A 29-day-old infant born of consanguineous parents had type 1 hyperlipoproteinaemia associated with lipaemia retinalis. Biochemical analyses disclosed hypertriglyceridaemia, hyperchylomicronaemia, and lipoprotein lipase deficiency. Ophthalmoscopically all retinal vessels had the creamy appearance of lipaemia retinalis. The plasma lipoprotein levels and fundus condition became normal after her ingestion of fat was limited. To our knowledge this is the youngest patient reported with type 1 hyperlipoproteinaemia associated with lipaemia retinalis.

Dietary Fats↗