Search PubMed⌕ Search

Biomedical subjects

Y Fukaya

Publications and source records attributed to Y Fukaya.

At least 73 records · Page 4Linked to original sources
PubMed1988

Effect of tannin on oxidative damage of ocular lens.

The protective effect of geraniin (tannin from Geranium thunbergii) against oxidative damage was examined in the mouse ocular lens. Oxidative damage in the lens was induced by diamide, diazene dicarboxylic acid bis (N,N-dimethylamide); diamide oxidized the sulfhydryl groups in both the membrane and cytoplasm but did not increase lipid peroxide. Geraniin showed protective effects on the changes in the Na+/K+ ratio, GSH level, Na,K-ATPase activity, GSH reductase activity and the sulfhydryl level of the membranous protein in the diamide-treated lens, but such protective effects of geraniin were not observed in the cell-free system of the lens. In addition, geraniin itself was unable to reduce GSSG to GSH and also unable to inhibit the oxidative reaction of the sulfhydryl group to diamide. These results suggest that in the intact lens geraniin would act primarily on the lens cell membrane surface to inhibit an influx of diamide into the inner part of the plasma membrane and the cytoplasm, and consequently that geraniin may protect sulfhydryl groups in the cell membrane and cytoplasm from their oxidation by diamide and keep the redox system of the lens in a normal state.

Animals↗
PubMed1988

[Metastatic tumor of the spermatic cord from renal cell carcinoma].

The patient was a 54-year-old man with a hard nodule (25 x 15 mm) in his left scrotum. His left spermatic vein had been ligated 6 months earlier because of a varicocele. Preoperative diagnosis was left renal tumor with multiple lung metastasis. The intrascrotal nodule was considered to be a metastatic tumor from renal cell carcinoma. Left nephrectomy was performed and the metastatic lesion of spermatic cord was also removed. Pathological examination showed that the left renal tumor was renal cell carcinoma (clear cell type). The spermatic cord nodule was tumor embolus in dilated veins and consisted of the same clear cells as renal cell carcinoma. Judging from these results, in this case renal cell carcinoma retrogradely metastasized through the spermatic vein to the intrascrotal lesion.

Carcinoma, Renal Cell↗
PubMed1987

Effects of tannins on the oxidative damage of mouse ocular lens. I. Using the oxidative damage model induced by the xanthine-xanthine oxidase system.

The protective effects of various tannins on ocular lens against the induced oxidative damage were examined. Oxidative damage on mouse lenses was induced by incubating them with xanthine-xanthine oxidase, ADP and Fe3+ (X.XOD system). X.XOD system caused an increase in lipid peroxide of lens membrane and decreases in Na,K-ATPase and GSH reductase activities in the lenses. After pretreatment of lenses with X.XOD system, the lenses were incubated with tannins in the medium containing no X.XOD system and the effects of tannins on biochemical parameters in the lenses were determined. Higher molecular tannins (penta-O-galloyl-beta-D-glucopyranose and geraniin) decreased the lipid peroxide in the lens and restored GSH content, Na,K-ATPase and GSH reductase activities in the lens to the level comparable to control. However, all of tannins tested restored much insufficiently the cation level (ratio of Na+/K+) in the lens regardless of extents of restoration of Na,K-ATPase level by them. Because it was supposed that tannins might act primarily on the plasma membrane, the effect of tannins on lens plasma membrane was examined using cell free system. Lens was homogenated and separated into membrane pellet and supernatant. When the pellet was treated with X.XOD system, the lipid peroxide in the pellet increased and its Na,K-ATPase activity decreased. In addition, the treated pellet decreased the GSH level and GSH reductase activity in the supernatant, when the pellet was combined with the supernatant. Higher molecular tannins reduced lipid peroxide content in the X.XOD-treated pellet to control level and the pellet in which lipid peroxide content was reduced by tannins caused much less decreases of GSH level and GSH reductase activity in the supernatant. These results suggest that, in intact lens, higher molecular tannins act on plasma membrane to eliminate lipid peroxide produced by the X.XOD system and consequently suppress the decreases in both Na,K-ATPase and GSH reductase activities without their entering inside the cell.

Animals↗
PubMed1987

[Neurogenic bladder due to the anterior spinal artery syndrome: 2 case reports].

A 43-year-old woman with anterior spinal artery syndrome was admitted with complaints of tetraplegia and urinary retention. Her bladder had capacity of more than 500 ml and was inactive, with low voltage of external urethral sphincter. She was treated with distigmine bromide and intermittent catheterization. 50 days later, urodynamic study showed normal bladder and incomplete coordination of external sphincter. A 78-year-old man had chief complaints of urinary retention and paraplegia. A catheter was left indwell for 6 months. Then he was treated with intermittent catheterization, but urgency incontinence was still observed. We discussed the therapy of neurogenic bladder due to the anterior spinal artery syndrome.

Adult↗
PubMed1986

A novel type of aryl sulfotransferase obtained from an anaerobic bacterium of human intestine.

A novel type of aryl sulfotransferase is produced by an anaerobic bacterium of human intestine, Eubacterium A-44. Aryl sulfotransferase separated from this bacterium differs from the sulfotransferase which uses 3'-phosphoadenosine 5'-phosphosulfate as a donor. The enzyme catalyzes stoichiometric transfer of a sulfate group from a phenol sulfate ester to other phenolic compounds, with strict specificity. The optimal pH and molecular weight were 8-9 and 315,000, respectively.

Arylsulfotransferase↗
PubMed1986

Effects of different weight loads on the body during motorcycle riding.

The authors examined the physiological changes of men riding motorcycles carrying different loads. Nine healthy professional riders were the subjects. They used professional Honda MD 90B-90CC motorcycles. Each subject made test rides with the loads of 0, 10, 15, and 20 kg, taken at random, on a concrete road for 20 min at a speed of 30 km/h. The environmental temperature at the time of the test, skin temperature, vibration sense threshold at a vibration level of 125 Hz, critical flicker fusion frequency, pinching power, grasping power, pulse rates, and blood pressure were measured. The pulse rates were significantly different for the rides with the four different loads, and the changes in pulse rate after the ride with the loads of 10 and 20 kg were comparatively higher than those before the ride. The changes of systolic blood pressure after the rides with the loads of 15 and 20 kg were significantly higher than those before the rides. The other changes observed were not significantly different.

Adult↗
PubMed1986

[An analysis of prognostic examination in cirrhotic portal hypertension and hepatoma].

With an increasing number of patients with advanced liver cirrhosis, the discrepancy between the preoperative examination and results of surgery for bleeding varices is widening. To correct this discrepancy, additional prognostic examinations to Child's criteria and routine hepatic laboratory tests were studied in our 246 cirrhotic patients with esophageal varices. These included wedged hepatic vein pressure, clearance and maximal removal rate of indocyanine green, and hepaplastin test. We performed the endoscope assisted terminal esophagoproximal gastrectomy with the EEA stapler gun with devascularization and splenectomy. No operative death and complications developed when the results of following 4 preoperative examinations were: wedged hepatic vein pressure below 400 mm of saline, peripheral disappearance rate (K) of indocyanine green above 0.04 min-1, maximal removal rate (Rmax) of the dye above 0.3mg/kg/min and hepaplastin test more than 40%. It is necessary for these indicators to be satisfied simultaneously prior to performing this surgery. In addition, these values should be changed a little in their critical limits when these cirrhotic patients also had hepatoma and were candidates for hepatectomy.

Adult↗
PubMed1985

Surgical treatment of skin depigmentation caused by burn injuries.

Thin split-skin autografts of normal color were performed on depigmented skin caused by second- and third-degree burns in 32 patients. Repigmentation appeared in almost all the recipient areas soon after grafting and no depigmentation occurred again in the treated area.

Adolescent↗
PubMed1985

Effect of islet cell autograft from the highly scarred pancreas on pancreatectomized dogs.

Ligation and section of the main and accessory pancreatic ducts were performed in adult mongrel dogs. Four weeks after the procedure we carried out total pancreatectomy in six duct-ligated dogs, and an attempt was made to prepare the graft of islets from the highly scarred pancreas of the duct-ligated dogs by means of a newly developed collagenase perfusion method. We succeeded to normalize the high fasting glucose level which occurred after the total pancreatectomy in three of six dogs through the autotransplantation of the procured graft. However, insulin secretion was severely depressed in transplanted animals. Two of the three dogs showed high plasma glucose levels 15 and 20 weeks after the transplantation, respectively. Histological examination of the spleen revealed well preserved islets with good immunocytochemical insulin staining in one transplanted dog whose plasma glucose level was controlled for about five months. No pancreatic exocrine tissue was detected in the histological sections of the spleen in the transplanted dogs and in a transplanted control dog.

Animals↗
PubMed1985

[Effects of urokinase on the transfer of 1-hexylcarbamoyl-5-fluorouracil (HCFU) into the blood, bile and pancreatic juice].

We studied effects of urokinase (Uronase) on the transfer of an oral anticancer agent, 1-hexylcarbamoyl-5-fluorouracil (HCFU) into blood, bile and pancreatic juice in 5 patients in whom pancreaticoduodenectomy has been performed for cancer of the periampullary region, and who had been simultaneously provided with drainage of the bile duct and pancreatic duct. Following oral administration of 500 mg of HCFU, HCFU and 5-FU concentrations in blood reached a peak at 2 hours, those in bile at 4 hours, and those in pancreatic juice at 4 to 6 hours. The administration of 24,000 IU of urokinase in combination with HCFU resulted in increased HCFU and 5-FU concentrations in blood, bile and pancreatic juice--the HCFU concentration in bile increased to 3 times and that in pancreatic juice, to about 5 times the level in the some counterparts in urokinase--untreated patients. These changes seemed to have resulted from the acceleration by urokinase of distribution of the anticancer agent into the organs.

Administration, Oral↗