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Biomedical subjects

Y Fukaya

Publications and source records attributed to Y Fukaya.

84 records · Page 5Linked to original sources
PubMed1984

[Study on the concentration of FT-207 and 5-FU in serum, lymph nodes and tissues after administration of FT-207 suppositories].

In 21 cancer patients receiving administration of FT-207 suppositories, we studied the concentration of FT-207 and 5-FU in the blood before surgery, and in the blood and tissues-tumor, normal and lymph node-sampled during surgery. These 21 cases were comprised of 12 cases of stomach cancer, 1 case of lymphosarcoma, 4 cases of rectal cancer, 2 cases of sigmoid cancer and 2 cases of breast cancer. Two hours after administration, the concentration of 5-FU reached its highest level and continued to maintain a level at which it could be expected to bring about clinical results even after four hours. The concentration of 5-FU in tumors and lymph nodes was high, especially in tumors compared with normal tissue (p less than 0.01). No difference could be found in the concentrations of FT-207 and 5-FU in serum and tissues from each 12 cases of stomach cancer, not only between of the early cancer and advanced cancer but also between patients more than 60 years old and those under 60 years old. No side-effects occurred in any case during suppository administration.

Adult↗
PubMed1984

[Assessment of the exposure of pest control operators to organophosphorus pesticides. Organophosphorus pesticides in blood and alkyl phosphate metabolites in urine].

Pest control operators usually spray pesticides in small areas such as a kitchen in a restaurant and are exposed to various pesticides, especially those of the organophosphorus (OP) type. In order to evaluate their occupational exposure to OP pesticides during the work, OP pesticides in blood and alkyl phosphate metabolites in urine of these operators were analyzed and the relationship between pesticide exposure and analytical results were studied. OP pesticides in blood were analyzed by gas chromatography with flame photometric detector (FPD-GC) after separation of phospholipid in blood with silicagel column chromatography. OP pesticides were not detected in any blood samples (the limit of detection was 1 ng/ml). Dimethylphosphate (DMP) and dimethylthiophosphate (DMTP), being urinary metabolites of OP pesticides, were analyzed by FPD-GC after benzyl derivatization. This method eliminated interfering peaks in gas chromatograms. The ratio of two isomeric derivatives of DMTP was found to be constant. Both DMP and DMTP of the exposed group were significantly higher than those of the non-exposed group, DMP being higher than DMTP. The ratio of DMP to DMTP in the fenitrothion-dichlorvos-exposed group was significantly higher than that in the fenitrothion-exposed group. It was considered that the ratio might reflect a result of pesticide exposure. The urinary metabolites of OP pesticides tended to become lower with the lapse of time since the last exposure. However, small amounts were detected in a few samples even 5 days after the last exposure.

Adult↗
PubMed1982

[Automated microdetermination of lead in capillary blood from earlobes by flameless atomic absorption spectrophotometry].

Recent development of flameless atomic absorption spectrophotometry has made possible more precise and sensitive determinations of metals including lead. This means that a sample size of microliter order of blood has come to give a sufficient analytic signal. Blood lead which has usually been determined using venous blood was determined in the present study using a small amount of capillary blood. The method developed is of lead determination in the capillary blood sample from earlobe by means of flameless atomic absorption spectrophotometry equipped with an automated microsampling system or autosampler. Thus, 70 microliters of heparinized whole blood sample from earlobe gave a satisfactory result in the lead analysis. The procedure is as follows. 1) Blood obtained from the earlobe is collected in a heparinized capillary tube. 2) The blood is diluted in the ratio of 1 : 9 with Triton 5000 X solution for complete hemolysis of erythrocytes, by which the matrix-bound lead is released and a better distribution of samples is made possible in the graphite tube. 3) Finally the sample is analyzed by a flameless atomic absorption spectrophotometer with an autosampler. Making sure the analytical method to be accurate and reliable for blood lead determination, the authors compared capillary blood lead levels (Pb-Bc) with venous ones (Pb-Bv) from the same subjects in the same sampling session. It was found that the correlation between Pb-Bc and Pb-Bv was highly significant (n = 144, r = +0.998, y = 0.97 x+0.32, p less than 0.001) and that they were almost of the same level. It was concluded that the method developed may be recommended for the routine clinical use.

Adult↗
PubMedDate not supplied

Improved patency of an elastomeric vascular graft by hybridization.

A newly devised hybrid graft with high antithrombogenicity for small caliber vascular grafts was developed. The design concept was based on the incorporation of a compliant open cell structured graft, autogenous endothelial cells (ECs), and artificial basement membrane. The latter, a gel complex of type I collagen and dermatan sulfate that showed enhanced adhesion and growth of ECs but reduced platelet adhesion, was coated onto a microporous polyurethane graft (internal diameter, 3 mm; length, 4.5 cm), with near-natural compliance. Ten seeded grafts were implanted bilaterally into the carotid arteries of dogs; anticoagulant or antiplatelet therapy was not administered. This hybrid graft showed a marked improvement in patency at 1 month compared with that of simply preclotted grafts (control specimens). This result was explained by the almost complete endothelialization when the graft was implanted, a high degree of adherent strength resistance to shear stress, and a high proliferative potential. Thus, this approach of combining biomechanical and cellular engineering designs may lead to an important functional small caliber graft.

Animals↗