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Biomedical subjects

Y Fujimoto

Publications and source records attributed to Y Fujimoto.

At least 487 records · Page 27Linked to original sources
PubMed1986

Glucosidation of estradiol-17 beta in the cultured ovaries of the silkworm, Bombyx mori.

Metabolic pathways of estradiol-17 beta and other vertebrate steroid hormones of cultured silkworm pupal ovaries were examined using 14C-labeled steroids. The isolated ovaries showed significant uptake and metabolic activity of the 14C-labeled estradiol-17 beta added to the medium. Analysis of the metabolized compounds by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed extensive metabolic conversion of [14C]estradiol-17 beta and estrone; i.e., estrone was reduced to estradiol-17 beta and estradiol-17 beta was metabolized to conjugates, including estradiol-3-beta-D-glucoside and estradiol-17-alpha-D-glucoside. [14C]Testosterone was not transformed appreciably by the ovaries. Metabolic activity and physiological significance of the vertebrate steroid hormones in the silkworm ovaries are discussed.

Animals↗
PubMed1986

The effects of reduced glutathione and cysteine on prostaglandin synthesis in rabbit kidney medulla slices.

The effects of GSH and cysteine on the generation of medullary prostaglandins E2 and F2 alpha were examined. The formation of prostaglandin E2 was enhanced by GSH, but was reduced by cysteine in a dose-related manner. GSH inhibited prostaglandin F2 alpha production, while cysteine stimulated it. These results suggest that GSH and cysteine have contrary effects on prostaglandins E2 and F2 alpha synthesis by affecting endoperoxide E2 isomerase or endoperoxide reductase.

Animals↗
PubMed1986

Further data supporting episodic degenerative discharge of thyroid hormone from nodular goiters.

To elucidate the features of thyroid hormone secretion under conditions of impaired circulation, blood samples were withdrawn at operation from thyroid and peripheral veins of 23 patients with thyroidal and non-thyroidal diseases. They were taken from the respective veins simultaneously, when the arterial supply to the thyroid tissue was partially occluded by surgical manipulation. It was found that triiodothyronine was preferentially, as compared with thyroxine, secreted from the thyroid, irrespective of the disease. Serum levels of thyroglobulin were also markedly elevated in the thyroid vein. These results suggest that episodic degenerative discharge of thyroid hormone from nodular goiters may be a cause of impaired or blunted TSH response to TRH, frequently observed in patients with common nontoxic nodular goiters.

Goiter, Nodular↗
PubMed1986

Antitumor effect of adriamycin entrapped in liposomes conjugated with monoclonal antibody against tumor-associated antigen of bovine leukemia cells.

Monoclonal antibody against tumor-associated antigen (TAA) expressed on bovine leukemia cells was conjugated to liposomes containing adriamycin (ADM), and the specificity and therapeutic effects of the conjugates were examined in vitro and in vivo using a TAA-positive bovine leukemia cell line as the target tumor. In vitro studies with the TAA-positive cell line clearly indicated that the antibody-conjugated liposomes containing ADM exerted selective effects on TAA-positive cells in the inhibition assay of 3H-thymidine incorporation. Three injections of liposomes containing ADM (4 mg/kg) into tumor-bearing nude mice significantly inhibited the tumor growth and the therapeutic effect of the antibody-conjugated liposomes was far greater than that of normal mouse IgG-conjugated liposomes as assessed in terms of tumor size.

Animals↗
PubMed1986

Justification of conservative surgical treatment of childhood thyroid cancer: report of eleven cases and analysis of Japanese literature.

Eleven thyroid carcinoma patients of 15 years old and under are reported. They represent only 1.3% of our personal series of 861 thyroid carcinoma cases. One case died from undifferentiated carcinoma of the thyroid. However, the other 10 cases of papillary or follicular carcinoma are all alive, in spite of the apparently advanced stage of the disease at the time of operation in most of the cases. From our experience and analysis of 127 cases of childhood thyroid cancer reported in the Japanese literature, it is suggested that childhood thyroid cancer constitutes an extreme case of thyroid cancer occurring in the adolescent and young adult periods, and that rather conservative surgical treatment, avoiding total laryngectomy, and preserving the recurrent laryngeal nerve and the parathyroid glands as far as possible, usually suffices for treatment. In these Japanese cases, radiation did not appear to have been an important causative factor of childhood thyroid cancer.

Adenocarcinoma↗
PubMed1986

Effect of methyl mercury on prostaglandin synthesis in rabbit kidney medulla slices.

Effects of methylmercuric chloride (MMC) in the absence and presence of ascorbic acid on the synthesis of prostaglandins E2 and F2 alpha, and the lipid peroxidation of rabbit kidney medulla slices were studied. MMC showed dose-dependent inhibition of prostaglandins E2 and F2 alpha formation. The lipid peroxidation was not stimulated by MMC alone. Ascorbic acid plus MMC were able to stimulate the lipid peroxidation markedly, but the lipid peroxidation induced by ascorbic acid plus MMC had no influence on prostaglandin E2 production decreased by MMC alone. These results suggest that MMC inhibits prostaglandin formation by affecting the activity of prostaglandin cyclooxygenase, but that lipid peroxidation is not directly responsible for this inhibitory effect of MMC.

Animals↗
PubMed1986

[Evaluation of prostate aspiration biopsy for cytological detection of prostate cancer].

The aspiration biopsy of the prostate for cytological detection was evaluated in 143 patients with clinically suspected prostate cancer and compared with the pathological diagnosis by needle biopsy performed simultaneously. Histopathological evaluation was possible in 136 cases. Sufficient cells for cytological diagnosis were obtained in 92.3% of pathologically diagnosed cases. Only 3 specimens for cytology (4.3%) of the prostate cancer before treatment were insufficient for cytology. Compared with pathological diagnosis by needle biopsy, false positive and negative rates were observed in 4.8% and 25.0% respectively. The accuracy rate of cytological diagnosis was higher the higher the degree of anaplasia in the pathological findings. In 20 clinically well-controlled patients the cytological results were negative in all of the well differentiated adenocarcinomas, whereas positive in poorly differentiated ones without exception. The cytological findings were still positive in all of the 4 cases with progressive cancer in spite of the anticancer therapy. Besides these results, because of no complication in the 182 aspiration biopsies, this may be a useful method for detection of prostate cancer, especially for screening of the cancer and judgment of efficacy during treatment.

Biopsy, Needle↗