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Biomedical subjects

W Lange

Publications and source records attributed to W Lange.

At least 199 records · Page 11Linked to original sources
PubMed1980

[Detection of hepatitis Be antigen with an enzyme immunoassay (author's transl)].

An enzyme immunoassay (ELISA) from SEROMED was tested for its suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis. From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.3-fold higherr than ELISA and 575-fold higher than rheophoresis. The differences between RIA and ELISA were shown to be due to somwhat lower sensitivity of ELISA. However, when sera from patients with Hepatitis B from various times after the onset of hepatitis (maximally 12 months) were tested, there was only a slight difference in sensitivity between ELISA and RIA. ELISA along with RIA is considered to be a substantially sensitive test for detection of HBeAG as compared to rheophoresis and can be recommended for routine use.

Blood Donors↗
PubMed1979

Comparative studies on the pre- and postterminal blood vessels in the cerebellar cortex of Rhesus monkey, cat, and rat.

In the rhesus monkey, cat and rat, pial arteries give off branches which run vertically through all three layers of the cerebellar cortex. The large cortical arteries are surrounded by a perivascular space in the molecular layer. Their wall consists of several layers of smooth-muscle cells and the luminal endothelium. As the arteries reach the deeper layers of the cerebellar cortex, the number of smooth-muscle cells is reduced. In the rat, sometimes no smooth-muscle cells are detectable in the preterminal arterial vessels. If these deep arteries branch off by dichotomy of terminal vessels there occurs a gradual or complete loss of myocytes in all three species. In the cat, where cortical arteries give off branches at right angles, there is a sphincter-like accumulation of smooth-muscle cells at the opening to the smaller branch. The postterminal vessels and veins in all species exhibit the same mural structure found in capillaries. The wall consists only of an endothelium and occasional pericytes embedded in the basal lamina. Even the large veins which run to the pial veins show this simple mural structure.

Animals↗
PubMed1979

Comparative evaluation of immunofluorescent (IgM and IgG) and complement-fixing antibodies in influenza A infection.

The indirect immunofluorescent (FA) tests for detection of IgG and IgM antibodies to the internal antigens of influenza viruses were performed using single cell suspensions of baby hamster kidney cells. The IgG-FA test showed a good correlation with the complement fixation (CF) test performed with purified ribonucleoprotein (RNP) as antigen than the CF test with whole virus antigen. Sera which were positive only in the IgM-FA test did not react in the RNP-CF test. A rapid diagnosis of influenza was not possible alone by detection of RNP specific IgM as relatively low titers were obtained in the IgM-FA test.

Antibodies, Viral↗
PubMed1978

[Demonstration of antibodies against HBs antigen with the modified MICROELISA-hepanostika-test (author's transl)].

Among various modifications of the Hepanostika test a simple, sensitive and reproducible one is described as being the best. Of 689 sera from patients in a haemodialysis unit 110 had antibodies against HBs antigen in the Hepanostika test and 150 in the Ausab test. Those antibodies which were not demonstrated in the Hepanostika test had a relatively low concentration (mean impulses per minutes 4.3 X NCx). Those sera which were positive in both the Hepanostika and Ausab test contained antibodies in higher concentrations (mean impulse/min 29.5 X NCx). Only 11 of the 689 sera were positive in the migration-electrophoresis test. A particularly advantage of the modified Hepanostika test is that both HBs antigen and antibodies against HBs antigen can be demonstrated simultaneously on the same plate. Using this method 200 sera were examined. The same sera were tested in the Austria-II-125 test for HBs antigen and the Ausab test for antibodies against HBs antigen. The Hepanostika test was positive in five, the Austria test in four sera. According to the Hepanostika test 43 sera contained antibodies against HBs antigen, while 45 did according to the Ausab test. The two that were positive only in the Ausab test were only weakly positive.

Antibodies, Viral↗
PubMed1978

The myelination of the cerebellar cortex in the cat.

The myelination of the cerebellar cortex of the cat was investigated in 61 cats aged from 3 hrs post partum to two and a half years. The first myelinated fibers appear at the time of birth in the central medullary ray. Before the onset of myelination, all fibers reach a critical diameter of about 1 micrometer. About the 14th day of life the number of oligodendrocytes in the prospective while matter increases markedly. Thereafter, the oligodendrocytes invade the inner granular layer. It therefore seems that the myelination of the cerebellar cortex proceeds from the central medullary ray towards the granular layer. At the 60th day of postnatal life, most of the afferent and efferent fiber systems are myelinated. These findings are discussed in relation to the development of function and the maturation of the electrical activity of the cerebellar circuit.

Animals↗
PubMed1978

Paired vessels in the spinal cord of Rhesus monkey and cat.

In the spinal cord of the rhesus monkey and of the cat paired vessels near the central canal are described. These paired vessels, consisting of one artery and one vein, are enveloped by a common glial sheath. In some Virchow-Robin's spaces two arteries and two veins can be observed. The physiological significance of this morphological feature is briefly discussed.

Animals↗
PubMed1978

[Histological studies on the hypothalamic adenohypophyseal-gonadal system after mestranol and chlormadinone acetate administration in fattening bulls].

Seventy-six young steers were examined to elucidate the action of the steroid oestrogen Mestranol and the gestagen chloromadinone acetate upon the morphology of hypothalamus, adenohypophysis, testes, and accessory sexual glands. The doses used were those applicable also to real animal husbandry. The mechanisms of action of the above pharmaceutical products are discussed against the background of the experimental results. No pathological alteration was observed that might prohibit application of the tested sexual steroids to young steers.

Animals↗
PubMed1977

[Demonstration of HBs antigen with the hepanostika test, an enzyme immunoassay (author's transl)].

Sensitivity, specificity and practicability of the Hepanostika test, a newly developed enzyme immunoassay by organon Teknika for the demonstration of HBs-antigen, was compared with results of the Aursria II 125 test. Smallest demonstrable concentration of the antigen in the Hepanostika test eas 3.125 ng/ml with antigens of subgroup ad and 2.08 ng/ml in those of subgroup ay, while it was 1.04 ng/ml for both subgroups with the Austria test. Among 2930 blood donors 85 antigen carriers were discovered by both tests, while with the Hepanostison test, a passive haemagglutination test from Oranon, only nine were discovered. In serial blood samples of patients with various disease there was only a slight difference between the two tests. The Hepanostika test had 1.6--3.88% nonspecific reactions, compared with 0.5-0.8% with the Ausria II 125 test. Both tests thus gave much more specific results than passive haemagglutination. The Hepanostika test is thus a satisfactory alternative to the Ausria test. Because of its high sensitivity it can be recommended for the diagnosis of HBs antigen among blood donors and in clinical laboratories.

Blood Donors↗
PubMed1977

[Possibilities in the use of anabolic substances as veterinary drugs for metaphylaxis and biotechnology].

An account is given of the latest developments and trends in using anabolic preparations in animal husbandry. The development of veterinary medicaments for metaphylaxis and biological engineering in industrialised animal husbandry is explained and substantiated, with reference being made to protein-anabolic and growth-stimulating properties. However, full observation of and compliance with codes and bylaws on drug and food production will depend strongly on efficient interdisciplinary socialist teamworks.

Anabolic Agents↗
PubMed1977

[Results of clinical tests on the fattening-stimulating action of the steroid estrogen mestranol in bulls].

Three experiments were conducted with 5 mg Mestranol per animal and die over 70 to 80 days and resulted in daily surplus gains of twelve, 14, and 18 per cent in terminal fattening of Frisian bulls. Also achieved were fodder savings by 1 kg dry matter and energy savings by 0.7 kEFr for each kilogram of gain as well as increase in slaughter weight by 8-9 kg per animal. Those favourable effects were attributed to anabolic and sexual-inhibiting action. A sedative effect on the nervous system is assumed. Combination with CAP failed to give any advantage regarding sexual inhibition and fattening result, but it rather entailed certain shortcomings, such as lower slaughter weight and stronger fat development. The development of an anabolic medicament for veterinary bio-engineering in terms of growth stimulation is suggested.

Abattoirs↗
PubMed1976

[Demonstration of hepatitis-B(surface) antigen with the Hepatest: a new passive haemagglutination test (author's transl)].

The Hepatest, a passive haemagglutination test, proved to be, like other tests of this group, significantly more sensitive for routine examination of hepatitis B(surface) antigen than tests of the first and second generation, but was not as sensitive as the Ausria II 125 test. The lowest HBs antigen concentration demonstrable in the Hepatest was 31 ng/ml for the subtype ad and 47 ng/ml for subtype ay (in the Ausria II 125 test lowest concentrations were 1.0 and 1.5 ng/ml, respectively). There were several false-positive reactions in the specificity test. Thus all positive reactions would need to be checked, more often than with the Ausria test. But the Hepatest proved to be the most practicable of all the third generation tests, both with regard to shortness of reaction time, technical requirements and ease of reading off the results.

Adult↗
PubMed1976

The myelinated parallel fibers of the cerebellar cortex and their regional distribution.

In the cerebellar cortex of the Rhesus monkey and the cat, the supraganglionic plexus in the molecular layer exhibits regional differences. The plexus is very well developed in the vermal parts of the anterior lobe, but only poorly developed in the nodulofloccular lobe. Most of the fibers of this plexus are myelinated parallel fibers, which synapse in the typical manner with dendritic thorns of Purkinje cells. Only very few fibers of this plexus are recurrent collaterals of Purkinje cells. Their distribution throughout the cerebellar cortex does not display regional differences. These findings agree with physiological data on the disinhibition of Purkinje cells in different parts of the cerebellar cortex.

Animals↗
PubMed1976

Speculations on a possible essential function of the gelatinous sheath of blue-green algae.

Voluminous and often fluffy sheaths surrounding blue-green algal cells are observed (a) in productive natural waters, (b) in bacteria-containing laboratory cultures growing in inorganic nutrient media with added bacteria-assimilable organic matter, and (c) in axenic cultures in the same inorganic media even without added organic matter. The sheaths of bacteria-associated species in inorganic media without added organic matter are, by comparison, thin, and growth is meager. Repeated observations show that voluminous sheaths and vigorous growth of algal species are associated. It is suggested that formation and retention of a voluminous shealth provide a microenvironment around the algal cell where essential nutrients, present at only submarginal levels in the surrounding water, are concentrated and become readily available to the cell. The increase in nutrient concentration above a critical level, in turn, leads to vigorous algal growth. The voluminous sheath produced by the alga is not attacked by alga-associated bacteria when other assimilable organic matter is available; but in the absence of a more suitalble food, the bacteria feed on the less desirable gelatinous sheath, markedly reducing its thickness and causing meager algal growth.

Bacteria↗