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Biomedical subjects

W Haase

Publications and source records attributed to W Haase.

At least 217 records · Page 12Linked to original sources
PubMed1977

Studies on isolated subcellular components of cat pancreas. I. Isolation and enzymatic characterization.

Pancreas of the cat was fractionated into its subcellular components by centrifugation through an exponential ficoll-sucrose density gradient in a zonal rotor. This enables a preparation of four fractions enriched in plasma membranes, endoplasmic reticulum, mitochondria and zymogen granules, respectively. The first fraction, enriched by 9- to 15-fold in the plasma membrane marker enzymes, hormone-stimulated adenylate cyclase, (Na+K+)-ATPase, and 5'-nucleotidase, is contaminated by membranes derived from endoplasmic reticulum but is virtually free from mitochondrial and zymogen-granule contamination. The second fraction from the zonal gradient shows only moderate enrichment of the above marker enzymes but contains a considerable quantity of plasma membrane marker enzymes and represents mostly rough endoplasmic reticulum. The third fraction contains the bulk of mitochondria and the fourth mainly zymogen granules as assessed by electron microscopy and marker enzymes for both mitochondria and zymogen granules, namely succinic dehydrogenase, trypsin and amylase. Further purification of the plasma membrane fractions by differential and sucrose step-gradient centrifugation yields plasma membranes enriched 40-fold in basal and hormone-stimulated adenylate cyclase and (Na+K+)-ATPase.

Adenosine Triphosphatases↗
PubMed1977

HLA frequencies in primary immunodeficiency diseases (pidd).

Thirteen patients with primary immunodeficiency disorders and their twenty-five healthy first-degree relatives were tissue typed and their HLA make-up was compared with that of a normal control population. HLA-A2 occured in 92.3% of patients as opposed to 60.8% in the control group (P less than 0.02), HLA-A9 in 7.6% vs. 25% (P less than 0.02) and HLA-B8 in 0% vs. 21% (P less than 0.04). One of the patients with severe combined immunodeficiency showed one "extraneous" HLA specifity.

Adolescent↗
PubMed1977

[The influence of quality of irradiation on survival in inoperable bronchial carcinoma (author's transl)].

Irradiation of inoperable bronchial carcinoma with constant ortho-volt technique has worse results than with mobile irradiation, since the individual dosage in several fields can be raised without damaging the skin. With mega-voltage limits of skin tolerance, changed absorption or protection of healthy tissues are absent, so that it is easier to deliver doses affective on the tumor. Survival-rates can be related to dosage. During the first few years similar doses of mega-voltage lead to more frequent survival. In part such results depend on differences in standard dosage with the various techniques. More important for the worse results of orthovoltage treatment are the higher doses, the greater volume of irradiation and scatter which may cause greater damage to immunity. 5 years survival of patients with a central inoperable bronchial carcinoma is hardly altered but during the first 2-3 years megavolt therapy is clearly more effective.

Carcinoma, Bronchogenic↗
PubMed1977

[Contribution to the radiation therapy of cerebral tumors (author's transl)].

Postoperative radiation therapy chiefly has to be regarded under the aspects of prevention or delay of recurrences. Primary radiation therapy without surgical extirpation of the principal mass of the tumor generally represents a palliative measure. As the radiosensitivity of a cerebral tumor is not exclusively dependent on the histological picture, histology should not influence the indication for radiotherapy. A diagnosis however, based on all clinical means, being reliable to a high degree, is a condition of the radiation therapy of brain tumors without a clear histologic determination. In this paper are reported the results of treatment from 132 intracranial tumors, and compared with data from literature.

Adenoma↗
PubMed1977

Systemic air embolism in experimental penetrating lung injuries.

Several reports have described systemic air embolism in association with penetrating lung injuries. We produce lung lacerations in 18 dogs ventilated with air containing charcoal powder. An ultrasonic bubble detector was positioned over the carotid artery in six dogs. Evidence of arterial air embolism was found in every case of pulmonary laceration in open-chest, tension pneumothorax, and hypovolemic preparations. The quantity of air was small, producing only transient arrhythmia. No air was detected under control conditions. We conclude that systemic air embolism is a frequent accompaniment of penetrating lung injuries, that it is rarely recognized clinically because of its generally small quantity and minor, nonspecific effects, but that the potential for dangerous air embolism does exist with lung wounds and warrants precautions in management.

Animals↗
PubMed1976

[Sitosterol in familial hyperlipoproteinemia type II. A randomized double-blind cross-over study].

The effect of beta-sitosterol on the lipid and lipoprotein level was evaluated in a randomised double-blind cross-over trial in 24 patients with primary familial type II hyperlipoproteinaemia over a period of 16 weeks. All patients completed the trial, however 10 of them had to be excluded from the evaluation due to fluctuations of their body weight or unreliable drug intake. Sitosterol lowered the total cholesterol level by 12.5% (P less than 0.01) from 9.96 mmol/l (3.69 g/l) to 8.37 mmol/l (3.23 g/l). The LDL-cholesterol level was lowered by 19.5% (P less than 0.05). The sitosterol concentration in plasma was consistently lower than 0.3% of total cholesterol. No side effects or tachyphylaxis was observed in the course of the trial. A return to normal of an increased serum cholesterol level by a combination of a lipid lowering diet and sitosterol monotherapy was only achieved in one patient.

Adolescent↗
PubMed1976

[Field study on the decrease of lipids using etofibrate].

A field-trial on Etofibrat was performed on 4405 patients suffering from primary and secondary hyperlipoproteinemia. The results were proved statistically. After a 3 to 4 weeks treatment the concentration of cholesterol as well as tryglicerides in the serum decreased significantly. After 6 to 8 weeks treatment the lipid-lowering effect was even stronger. Nearly 90% of the patients investigated gave a positive response to the treatment. Part of the population had undergone a treatment with other lipid-lowering agents before-most likely without sufficient success-in these cases a further lipid-lowering effect due to Etofibrat could be shown. Under-dosage in premedication cannot be excluded. The stratification of the patients in different groups of diagnosis showed a nearly similarity of both blood lipids independent of the diagnosis. This could also be confirmed for the group of patients suffering from diabetes. To prove the lipid-lowering efficacy of Etofibrat a population was withdrawn from treatment. Cholesterol as well as tryglicerides increased significantly during the interval without treatment. During a long-term study both lipid fractions could be kept down without increasing of the daily Etofibrat dose. The tolerance of Etofibrat was stated to be good up to very good. Objectively the measured enzymes SGOT, SGPT and gamma-GT showed a decrease of the means. Subjectively the occurrence of an often intermediate heat sensation and/or rubedo was of relevance. The low daily dose compared with other lipid-lowering agents gives indication for a better pharmacocinetic behaviour of the drug;

Alanine Transaminase↗
PubMed1976

[Therapy of cervico-brachialgia. Controlled clinical comparison of a high-dose combination of neurotropic vitamins with an analgesic].

The study revealed that cervicobrachialgias (cervical syndrome) can be effectively treated with high-dosed neurotropic vitamins. This refers in particular to pain caused by pressure on the cervical plexus and paravertebral pain on pressur, Regarding paravertebral pain on pressure, it was possible to prove a significant superiority of the therapy with Neurotrat forte. The duration of therapy in both groups was, however, too short in order to prove a significant improvement regarding the lateral listing of the cervical spine. Both forms of treatment produced similar results in view of an improvement of impaired sensibility, paresthesia and alleviation of pain. In both groups side effects were only occasionally observed.

Adult↗