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Biomedical subjects

V K Sharma

Publications and source records attributed to V K Sharma.

At least 397 records · Page 22Linked to original sources
PubMed1978

alpha-adrenergic receptor in rat heart. Effects of thyroidectomy.

The effects of thyroid status on alpha-adrenergic receptors in the rat myocardium were investigated. The potent antagonist [3H]dihydroergokryptine was used to identify alpha-adrenergic receptors in rat heart particulate and sarcolemmal fractions. Administration of triiodothyronine to thyroidectomized rats decreased specific binding to alpha-adrenergic receptors in heart particulate and sarcolemmal fractions by 41% and 45%, respectively. Scatchard analysis revealed that the cardiac sarcolemmal fraction from thyroidectomized rats contained 29.3 fmol/mg of protein, as compared with 17.0 fmol/mg of protein found in the heart preparation of thyroidectomized rats treated with triiodothyronine. The equilibrium dissociation constants for the interaction of receptors with dihydroergokryptine were similar (about 1.5 nM) in the heart sarcolemmal fractions derived from these two groups of rats. The results of this study demonstrate that thyroid hormone can regulate the number of cardiac alpha-adrenergic receptors. In addition, there appears to be a reciprocal relationship between alpha-adrenergic and beta-adrenergic receptors in the rat myocardium.

Animals↗
PubMed1978

beta-Adrenergic receptors in rat skeletal muscle. Effects of thyroidectomy.

Thyroid hormones may participate in the regulation of beta-adrenergic receptors in skeletal muscle sarcolemmal membrane. Since skeletal muscles are not innervated by sympathetic nerve endings, the biochemical mechanism involved in the control of beta-adrenergic receptors by thyroid hormones appears to be mediated by thyroid-induced regulation of serum levels of catecholamines.

Alprenolol↗
PubMed1978

Enterobacteria of emerging pathogenic significance from clinical cases in man and animals and detection of toads and wall lizards as their reservoirs.

A total of 416 samples comprising faecal samples from diarrhoeic cases of man, calves, sheep and goats, and urine samples from patients with urinary tract infections, were examined for the presence of enterobacteria of emerging pathogenic significance. Citrobacter freundii from 20, C. intermedius biotype-a from four, Serratia marcescens (serotype 05:H13, bactericin type 16) from one and Erwinia herbicola from two human stool samples were isolated. Only two urine samples yielded C. freundii. Citrobacter freundii was isolated from 10 and C. intermedius biotype-a from two calves. From sheep and goats, two isolates of C. freundii and three of C. intermedius biotype-a were obtained. None of these samples yielded Edwarsiella tarda or Yersinia enterocolitica. The examination of 99 toads and 145 wall lizards revealed that toads were reservoirs for C. freundii, C. intermedius biotype-a and Salmonella brijbhumi, whereas wall lizards were reservoirs for C. freundii, C. intermedius biotype-a, E. herbicola, Enterobacter cloacae and Salmonella spp. These bacteria were present in the range of 2.0 x 10(6) to 6.0 x 10(11) organisms per gram of intestinal contents. In addition, toads were carriers for Edwardsiella tarda (new serotypes 04167:H1 and 05159: non-motile). None of the toads and wall lizards proved positive for C. intermedius biotype-b (C. koseri), S. marcescens and Y. entercolitica. C. freundii, C. intermedius biotype-a, E. herbicola and S. marcescens were resistant to penicillin and erythromycin whereas E. tarda isolates were also resistant to gentamycin, neomycin, colistin and sulfamethaxazole.

Animal Diseases↗
PubMed1978

[3H]-Ouabain binding to peripheral organs of cats: effect of ethanol.

1 The specific [3H]-ouabain binding to microsomal fractions derived from cat heart, liver, spleen, and kidney increased significantly following chronic administration of ethanol. 2 Since ouabain binds exclusively to cell membrane (Na+ + K+)-adenosine triphosphatase ((Na+ + K+)-ATPase), these results provide evidence for an increase in number of (Na+ + K+)-ATPase macromolecules during chronic alcoholism. 3 The importance of the increase in number of (Na+ + K+)-ATPase molecules in the adaptive increase in ethanol metabolism and cardiac myopathy in chronic alcoholism is discussed.

Adenosine Triphosphatases↗
PubMed1977

Muscarinic cholinergic receptors in rat heart. Effects of thyroidectomy.

The effects of thyroid status on muscarinic cholinergic receptors in the rat myocardium were investigated. The potent muscarinic antagonist [3H]quinuclidinyl benzilate was used to identify muscarinic cholinergic receptors in rat heart particulate fractions from control, hypothyroid, and hyperthyroid rats. Thyroidectomy increased specific [3H]quinuclidinyl benzilate binding to heart particulate fractions by about 60% as compared to euthyroid rat cardiac preparations. Administration of triiodothyronine to euthyroid rats decreased specific binding to muscarinic cholinergic receptors by about 20%. Scatchard analysis revealed that the cardiac particulate fraction from thyroidectomized rats contained 134 fmol of [3H]quinuclidinyl benzilate binding sites per mg of protein, as compared with 85 fmol/mg of protein found in the heart preparation of thyroidectomized rats chrinically treated with triiodothyronine. The equilibrium dissociation constant for the interaction of receptors with quinuclidinyl benzilate was the same (1 nM) in the heart particulate fractions derived from these two groups of rats. The results of this study demonstrate that thyroid hormone can regulate the number of cardiac muscarinic cholinergic receptors. Thus, the parasympathetic nervous system may participate in the cardiovascular abnormalities in different thyroid states.

Animals↗