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Biomedical subjects

V K Sharma

Publications and source records attributed to V K Sharma.

At least 415 records · Page 23Linked to original sources
PubMed1977

[3H]-ouabain binding to denervated and innervated skeletal muscle.

In cat skeletal muscles, the major part of specific [3H]-ouabain binding was found in the sympathetic nerve endings of red, slow muscle fibres. Skeletal muscle denervation increased specific [3H]-ouabain binding to muscle membrane preparation. This increase may be involved in the development of spontaneous fibrillation in the denervated muscle.

Adenosine Triphosphatases↗
PubMed1977

Inhibition of [3H]norepinephrine uptake in peripheral organs of some mammalian species by ouabain.

In order to demonstrate the possible involvement of (Na+ + K+)-ATPase in the high affinity uptake of [3H]-norepinephrine in the sympathetic nerve endings, the effect of ouabain on [3H]norepinephrine uptake in spleen and heart slices of five mammalian species was examined. The ouabain sensitivity of [3H]norepinephrine uptake in the heart slices form various species, as determined by the estimation of IC52, was, in increasing order, lamb (2,3 muM) less than calf (2.5 muM) less than guinea pig (4 muM) less than rabbit (10muM) less than rat (greater than 500 muM). The IC50 values in the spleen slices were: lamb (1 muM) less than calf (3.2 muM) less than rabbit (9.5 muM) less than guinea pig (25 muM) less than rat (greater than 500 muM). The IC50 values for the inhibition of specific [3H]ouabain binding in the microsomal fractions of spleen and heart of the five mammalian species by ouabain were similar to the IC50 values for the inhibition of [3H]norepinephrine uptake by the cardiac glycoside. Since ouabain is known to bind exclusively to (Na+ + K+)-ATPase of a microsomal fraction, these results suggest that the inhibition of [3H]norepinephrine uptake in the sympathetic nerve endings by ouabain is mediated by the inhibition of (Na+ + K+)-ATPase.

Animals↗
PubMed1977

Toads as natural reservoir of salmonella.

The investigation conducted on 329 toads for the presence of Salmonella, Arizona and Edwardsiella revealed that 36.7% toads were intestinal carriers of Salmonella. None of the toads proved positive for Arizona and Edwardsiella. The Salmonella population (S. brijbhumi) in the intestines of 5 of the 27 toads ranged from 1.5 X 10(9) to 2.5 X 10(10) organisms per gram of faecal matter. Some of the serotypes viz: S. tel-hashomer, S. brijbhumi and S. goverdhan were recovered more frequently than others. An unnamed serotype (4, 12, 27:k:-) and S. goverdhan var 1 + (1,9,12:k:1,6), hitherto undescribed in the literature, have also been reported.

Animals↗
PubMed1976

Evidence of morphological and physiological transformation of mammalian cells by strong magnetic fields.

Cultures of L-929 and WI-38 cells, frozen to 4.2 degrees K and exposed for 4 to 8 hours to 5000-oersted magnetic fields, were markedly inhibited in their growth as compared to controls. In cultures grown on cover slips, approximately 7 days after exposure, morphologically distinct cells emerged and were propagated from generation to generation; 3 weeks later, in flask cultures, contact inhibition was abolished. It is concluded that under certain experimental conditions, strong magnetic fields induce morphological and physiological transformations of target cells.

Cell Division↗