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Biomedical subjects

V K Gupta

Publications and source records attributed to V K Gupta.

At least 127 records · Page 7Linked to original sources
PubMed1989

Effect of experimental zinc deficiency and repletion on sodium, potassium, copper and iron concentrations in guinea-pigs.

Zinc, sodium, potassium, copper and iron concentrations were analysed in serum and tissues of guinea-pigs fed on a diet containing 1.25 mg Zn/kg diet over a period of 60 d. The response of the Zn-deficient (ZnD) animals to Zn supplementation (100 mg Zn/kg diet) was also studied for 15 d. Serum studies in the ZnD group revealed significant decreases in the concentrations of Zn and Na from 24 d, and increases in the concentrations of Fe and K from 36 and 48 d onwards respectively; an increase in Cu was seen on day 60 only. Zn deficiency caused significant reductions in Na, K and Zn and increases in Cu and Fe contents of liver and kidney. In testis, significant decreases were noted only in Zn, K and Fe contents. Zn supplementation of the previously ZnD group resulted in marked improvements in serum and tissue mineral levels. However, hepatic Cu and Fe and renal K did not appear to respond appreciably.

Animals↗
PubMed1989

Fecundity of two hillstream fishes, Garra lamta (Ham.) and Garra gotyla gotyla (gray).

The fecundity of two hillstream fishes, Garra lamta and G. gotyla gotyla, is related to their total body length (L), total body weight (W), ovary length (X) and ovary weight (V). The relationships to L, W, X and V were all linear. The correlation coefficient values (r) showed that total body length and total body weight were the best indices in fecundity estimates in both fishes.

Animals↗
PubMed1988

Partial Purification and Characterization of NADP-Isocitrate Dehydrogenase from Immature Pod Walls of Chickpea (Cicer arietinum L.).

NADP(+)-isocitrate dehydrogenase (threo-DS-isocitrate: NADP(+) oxidoreductase [decarboxylating]; EC 1.1.1.42) (IDH) from pod walls of chickpea (Cicer arietinum L.) was purified 192-fold using ammonium sulfate fractionation, ion exchange chromatography on DEAE-Sephadex A-50, and gel filtration through Sephadex G-200. The purified enzyme, having a molecular weight of about 126,000, exhibited a broad pH optima from 8.0 to 8.6. It was quite stable at 4 degrees C and had an absolute requirement for a divalent cation, either Mg(2+) or Mn(2+), for its activity. Typical hyperbolic kinetics was obtained with increasing concentrations of NADP(+), dl-isocitrate, Mn(2+), and Mg(2+). Their K(m) values were 15, 110, 15, and 192 micromolar, respectively. The enzyme activity was inhibited by sulfhydryl reagents. Various amino acids, amides, organic acids, nucleotides, each at a concentration of 5 millimolar, had no effect on the activity of the enzyme. The activity was not influenced by adenylate energy charge but decreased linearly with increasing ratio of NADPH to NADP(+). Initial velocity studies indicated kinetic mechanism to be sequential. NADPH inhibited the forward reaction competitively with respect to NADP(+) at fixed saturating concentration of isocitrate, whereas 2-oxoglutarate inhibited the enzyme noncompetitively at saturating concentrations of both NADP(+) and isocitrate, indicating the reaction mechanism to be random sequential. Results suggest that the activity of NADP(+)-IDH in situ is likely to be controlled by intracellular NADPH to NADP(+) ratio as well as by the concentration of various substrates and products.

Journal Article↗
PubMed1987

The standardization of the reverse indirect haemagglutination test for the assay of the viral antigen of Japanese encephalitis vaccine.

The reverse indirect haemagglutination (RIHA) test has been standardized for the assay of the viral antigen of purified Japanese encephalitis (JE) vaccine. Glutaraldehyde fixed sheep erythrocytes were sensitized with ammonium sulphate purified antibodies to JE vaccine raised in mice. The sensitivity of the erythrocytes fell to about one hundredth of the initial sensitivity in the first two days after preparation. After initial loss in sensitivity the stability of the cells became stabilized and the cells retained their titre for one year at 4-8 degrees C. The initial loss in sensitivity was not reduced by storing the cells at -70 degrees C, but after freeze drying the sensitized cells with a stabilizer one day after their preparation the cells retained their sensitivity. The RIHA test has been found to be a highly reproducible and sensitive method for detecting viral antigen in 5-10 ng of protein nitrogen. The sensitivity of the test was affected by the origins of the erythrocytes, i.e. from the different sheep from which they were drawn. To obtain results more rapidly, goose erythrocytes were used in place of sheep erythrocytes and the sensitized goose erythrocytes gave RIHA results in only 40 min.

Animals↗
PubMed1985

Storage iron in human foetal organs.

Maternal plasma iron and storage iron (non-heme) in the foetal liver were estimated in 108 foetuses whose gestational ages ranged from 12-44 weeks. Simultaneous estimations of storage iron were also made on the spleen and kidney in 70 and 69 of these foetuses respectively. Although the concentration of iron per g wet tissue in these organs remained practically the same at all gestations, the total storage iron content increased with the increase in gestation due to growth of these organs. The liver, in particular, showed a steep rise in total iron content in the last 8 weeks of gestation. There was a significant positive correlation between the total storage iron content in the foetal organs and the birth weight and gestational age of the foetus, suggesting that the preterm infants had smaller iron stores at birth as compared to infants born at term. The direct relation of the concentration as well as the total content of storage iron in the foetal liver with the maternal plasma iron levels suggested that babies born to iron deficient mothers had poor iron stores in their livers.

Birth Weight↗