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Biomedical subjects

V I Skvortsova

Publications and source records attributed to V I Skvortsova.

At least 37 records · Page 2Linked to original sources
PubMed2004

The effects of hormones of the hypothalamo-hypophyseal-adrenal, renin-angiotensin, and thyroid hormone systems on the formation of dyscirculatory encephalopathy.

Measurements were made of plasma levels of free (f) thyroxine (fT4), triiodothyronine (fT3), thyrotropic hormone (TSH), adrenocorticotrophic hormone (ACTH), aldosterone, and renin in patients with dyscirculatory encephalopathy (DE). Their influences on the development of chronic circulatory insufficiency were assessed. A total of 39 patients were studied (aged 45-73 years) with DE stages I and II, without acute or chronic (in the exacerbation phase) somatic illness. These observations showed that diffuse lesions of brain tissues of different severities were accompanied by the following changes in thyroid homeostasis: 1) significant combined increases in TSH without alteration to the "fT3-TSH" negative feedback regulatory mechanism in patients with stage I DE; 2) significant combined decreases in TSH levels with marked suppression of the conversion of thyroxine into triiodothyronine and an interaction with impairments in the "fT3-hypophysis" system in patients with stage II DE. In addition, there were changes (increases) in cortisol levels with simultaneous decreases in renin levels in patients with stage II DE as compared with patients with stage I DE. Correlation analysis demonstrated the absence of any relationship between the age of the patients, the state of hormonal homeostasis, and the extent of vascular stenosis. These results suggest a role for hormones of the hypothalamo-hypophyseal-adrenal, thyroid, and renin-angiotensin systems in the mechanism by which DE develops as well as the possibility of using tests for these hormones as additional criteria for assessing the severity of diffuse brain lesions.

Aged↗
PubMed2004

[Possibilities and perspectives of berlition usage in the treatment of alcohol polyneuropathy].

A comparison study of efficacy and tolerability of the drug berlition 300 oral and berliton 300 U and vitamin B1 has been conducted in 56 patients with alcohol polyneuropathy (15 female, 41 male, mean age 42.6 years). The key mechanisms of berlition action are increasing of endoneural blood flow, strengthening of antioxidant system functioning and reduction of "oxidative stress" intensity; improvement of glucose consumption and restoring of nerve energetic balance as well as intensification of nerve growth factor releasing and nerve growth acceleration after its experiment cutting or squeezing. Usage of berlition in the cohort studied was accompanied by a positive dynamics of both subjective and objective clinical symptoms. Comparing to vitamin B1, the drug was significantly more effective by clinical and electrophysiological indices. Berlition therapy is tolerable and safe. The results of the study confirm an assumption of berlition efficacy in alcohol polyneuropathy and allow to recommend the drug for a wide clinical application.

Adult↗
PubMed2004

Connection between p53 gene Bam HI RFLP polymorphism with the volume of brain infarction in patients with carotid atherothrombotic ischemic stroke.

PURPOSE: The aim of our investigation was to study the connection between p53 gene Bam HI RFLP polymorphism and the brain infarction volume in patients with atherothrombotic ischemic stroke that could highlight certain genetic aspects of the individual sensibility of brain tissue to acute ischemia. MATERIALS AND METHODS: Diallelic Bam HI RFLP polymorphism in 5' flanking region p53 gene was studied in 96 patients with carotid atherothrombotic stroke from Moscow population. Magnetic resonance imaging was conducted on day 7 after the stroke onset. The manual morphometry and "Osiris" morphometric hardware (by the Hospital of the University of Geneva) were used for assessment of the infarction volume. RESULTS: The predominance of small-size infarctions (< 40 cm3) was revealed in patients with (-/-) Bam HI RFLP p53 genotype versus patients with (-/+) (X2 = 19.7; P < 0.001) and (+/+) (X2 = 12.288; P < 0.001) genotypes. According to the Bayesian's statistics, in patients with (-/-) p53 Bam HI genotype the development of a small-size infarction in atherothrombotic ischemic stroke can be prognosticated with probability more than 65%. CONCLUSIONS: A significant association between p53 gene Bam HI RFLP polymorphism and the infarction volume was found in patients with carotid atherothrombotic stroke from Moscow population. These results additionally confirm that apoptosis plays an important role in the formation of ischemic brain lesion and that drugs with anti-apoptotic properties may prove beneficial in stroke patients.

Acute Disease↗
PubMed2004

[Analysis of the glutathione S-transferase P1 gene Ile105Val polymorphism in the patients with sporadic motor neuron disease from Russia].

Ile105Val polymorphism in exon 5 of glutathione S-transferase (GSTP1) gene was examined in a group of patients with motor neuron disease (MND) and control sample. No statistically significant differences in the allele and genotype frequency distributions between the samples examined were demonstrated. We conclude that Ile105Val polymorphism is not associated with the risk of the disease development in the patients from Russia with sporadic form of MND.

Alleles↗
PubMed2004

[The results of the MATCH study evidence for advantages of Clopidogrel monotherapy as secondary prophylaxis in cerebrovascular pathology].

A multicenter randomized double-blind placebo-controlled clinical trial The Management of Atherothrombosis with Clopidogrel in High Risk Patients (MATCH) with Transient Ischemic Attack (TIA) or Ischemic Stroke (IS) studied efficacy and safety of a co-medication with acetylsalicylic acid (ASA) and Clopidogrel in comparison with a Clopidogrel monotherapy. It is shown that an addition of ASA to Clopidogrel therapy did not result in significant reduction of the risk of vascular events in patients with cerebrovascular pathology. But comparing to the placebo group, there was a significantly higher frequency of life-threatening bleedings [96 cases (2.6%) versus 49 (1.3%); p < 0.001] and pronounced bleedings [73 cases (1.9%) versus 22 (0.6%); p < 0.001] in the ASA receiving group. Therefore, the results of the MATCH trial demonstrated that ASA did not have any additional benefits while added to Clopidogrel it essentially raised a number of side effects.

Aged↗
PubMed2004

[A randomized, double-blind, placebo-controlled study of Cerebrolysin safety and efficacy in the treatment of acute ischemic stroke].

The aim of the study was to assess safety and efficacy of the neuroprotective drug Cerebrolysin in acute ischemic stroke. Thirty-six patients with ischemic stroke in carotid artery territory aged 45-85 years, were eligible for inclusion in the trial if they were admitted to the hospital within the first 12h after stroke onset. Patients were randomly and blindly assigned to placebo (n = 12) or 1 or 2 dosages of Cerebrolysin: 10 ml/d (n = 12) and 50 ml/d (n = 12) for 10 days with concomitant standard basic treatment in each group. A quantitative time-related analysis of the dynamics of neurological deficit revealed the tendency towards acceleration of improvement assessed by the Clinical Global Impression Scale and NIHSS in both Cerebrolysin groups by 30 day of the treatment. The significant reduction in the volume of MRI ischemic focus was shown in both Cerebrolysin groups (p < 0.05 vs Placebo) on day 3. Acute pharmacological test revealed a decrease (p < 0.05 vs Placebo) of the size and spread of delta and theta foci in 72.7% patients, receiving 50 ml/d of Cerebrolysin. In none of the cases, Cerebrolysin treatment provoked any paroxysmal activity on EEG. The trial demonstrated safety, efficacy and good tolerability of hige-dose Cerebrolysin in the treatment of ischemic stroke.

Acute Disease↗
PubMed2004

[Free radicals in cerebral ischemia].

The review considers an involvement of active oxygen forms, nitric oxide (NO) and its active forms in accumulation of low molecular weight cytotoxic compounds that lead directly to cell death. A dual role of free radicals in cerebral ischemia, free radical function as secondary messengers are analysed.

Brain↗
PubMed2004

[Cryoglobulinemia and extracorporal plasma therapy in severe ischemic stroke].

Forty-five patients with carotid ischemic stroke have been examined within the first 24 hours from the disease onset. The Orgogozo and NIH scales were used to objectify a severity of the patient's state and the Barthel scale--to evaluate a degree of functional rehabilitation. Cryoglobulins (CG) were measured in blood serum by spectrophotometric method on the 1st, 2nd, 3rd, 7th and 21st days. Selective plasmapheresis was included in the therapeutic complex for 22 randomly selected patients. In both groups, a level of CG positively correlated with a severity of the patient's state on the 1st day of the monitoring (r=-0.5; p<0.01). The study of hemostasis indicated the presence of a direct correlation between an increase of platelet aggregation, blood hyperviscosity and CG concentration (r=0.46; p<0.05 and r=0.74; p<0.01, respectively). Higher scoring on the Orgogozo scale and Barthel scale prevailed in the group treated with plasmapheresis that significantly correlated with a decrease in CG concentration in blood serum (r=-0.6; p<0.05 and r=0.5; p<0.05 respectively). An evaluation of platelet aggregation, blood viscosity and a coagulogram on the 21st day of the disease revealed a significant improvement of blood rheology in the group of patients switched to efferent therapy.

Acute Disease↗
PubMed2004

[Long-term survival of poststroke patients and problem of the cardiovascular risk factors control according to the data of Krasnodar stroke register].

Out of 254 patients included in Krasnodar stroke register in the last half-year of 1997, 48.8% survived one year, 26.8% were alive by June 2003 and 5.5% have left the region. A dynamics of the prevalence of the risk factors for secondary stroke during 5.7 +/- 0.2 years indicated a significant increase of cardiovascular system lesion. However, the corrective means of major modifying cardiovascular risk factors substantially differed from those recommended by the results of large randomized clinical studies conducted over the last years. Use of detected reserves of secondary prophylaxis in patients who survived stroke is restricted by organizational and economic problems.

Aged↗
PubMed2004

[The dynamics of recovery processes in ischemic stroke as shown by comparison of clinical and tomographic indices].

The aim of this study was to assess the relation between MRI-changes of the brain infarction (BI) and neurological status in patients with hemispheric ischemic stroke. Clinical and MRI examinations were performed in 70 patients (aged from 46 to 89 years, 37 females, 33 males) 1, 3, 7-10, 14 and 21-30 days (in all cases) and 2, 6 and 12 months (n=29) after stroke onset. On FLAIR-images the criteria of assessment of cystic and gliosis transformation were determined. The tissue contrast of BI is assigned mainly to brain edema and hemorrhagic transformation, the cystic and gliosis transformation visualized in 1-2 months after stroke onset. The ratio between cystic and gliosis transformation correlated with clinical dynamics and the size of BI.

Aged↗
PubMed2004

[Protein HLDF and antibodies to it as molecular pathogenetic factors and new markers of acute cerebral blood circulation disturbances].

Clinic-experimental complex investigation and ELISA determination of protein HLDF as also primary antibodies (Abs) to HLDF values in blood and cerebro-spinal fluid (CSF) of patients with hypertensive crises and acute aterotrombotic ischemic stroke were performed. Statistically improved difference in serum HLDF and Abs content in examined patients in comparison with age-matched controls as also significant intergroup's differences in protein and Abs dynamic content were determined. Correlation between content and dynamics of investigated factors in CSF and blood serum with determined clinical and instrumental parameters was revealed. Pathogenetic and prognostic significance of revealed changes in molecular factors was viewed.

Acute Disease↗
PubMed2003

Neurophysiological characterization of transitory global amnesia syndrome.

A total of 27 patients with transitory global amnesia (TGA) in the acute and late (from 7 days) periods and 31 patients with dyscirculatory encephalopathy and subjective memory impairments (control group) were studied. EEG data and assessment of the P300 cognitive evoked potential wave established differences in the nature of beta1 activity between these groups. The extent of beta1 activity on the EEG showed different relationships with the latent period of the P300 wave: in the control group, there were increases in beta1 activity with increases in the latent period (r = 0.43). while beta1 activity in TGA decreased with increases in latent period (r = -0.23). These changes were most marked in the frontocentral areas. The authors believe that these patterns of changes in EEG and cognitive evoked potentials in the acute period of TGA and at later periods, as well as in the control group, provide evidence of the functional nature of TGA syndrome, which is not related to any damaged brain structure.

Aged↗
PubMed2003

[Role of missense mutation (M235T) in the angiotensinogen gene in development of cerebral ischemia].

Possible correlation of M/T polymorphism of angiotensinogen gene with risk of ischemic stroke and basic risk factors of cerebral pathology (levels of arterial pressure and blood cholesterol; presence of diabetes mellitus, coronary heart disease, or myocardial infarction in anamnesis; and stenosis of major cerebral arteries) was studied. It was shown that M/T polymorphic variants of angiotensinogen gene were factors determining neither clinical variant of cerebral ischemia development (acute ischemic stroke or chronic brain ischemia) nor formation of main risk factors of stroke.

Aged↗
PubMed2003

[Association of homozygosity for short allele (S) of heavy neurofilament subunit gene with motor neuron disease and oxidative stress development].

We analyzed distribution of heavy neurofilament (NF-H) gene S/L-polymorphic variants in 51 patients with idiopathic motor neuron disease (MND) vs control group and in relation to superoxide dismutases (SODs) activity and thiobarbituric acid reactive substances (TBARS) level in cerebrospinal fluid (CSF), erythrocytes and blood serum. We found that individuals with homozygosity for NF-H gene short allele (S/S-genotype carriers) in MND group predominate significantly over those in control one (p < 0.001). We revealed significant increase of oxidative markers in CSF and blood serum in MDN patients vs controls (p < 0.05), but not in patients with spondylogenic myelopathy, conforming non-specific role of oxidative stress in MND pathogenesis. There were no differences between TBARS level in CSF and serum in relation to the rate of MND progression, suggesting that oxidative stress does not influence the MND course. We showed normal SOD-1 activity in erythrocytes and CSF of MND patients that argued for the absence of these antioxidant enzymes deficiency in MND without SOD-1 gene mutations. We found significant association between homozygosity for short allele (S) and increased TBARS level in CSF (p < 0.02). These findings specify the role of NF-H with lower molecular weight in MND pathogenesis and make expedient antioxidants administration to MND patients homozygous for S-allele of NF-H gene.

Alleles↗
PubMed2003

[Epidemiology of stroke in Russia].

Using a register approach, an epidemiologic survey of stroke in Russia was conducted according to a program specially worked out. A total sample studied included 2,398,498 subjects, aged 25 years and older from 19 Russian cities. Overall, 9998 new cases of acute blood supply disturbances were registered for a one-year period. In 2001, stroke morbidity was found to reach 3.36 per 1000 persons, mortality rate--1.28, lethality--40.37% (61.4% in hemorrhagic and 21.8% in ischemic stroke). The highest morbidity was observed in the North-West regions (7.43 per 1000), in some cities of middle areas (5.37) and in the Far East (4.41), percentage of hemorrhagic stroke being higher as well. It is concluded that a longitudinal register for obtaining data on mortality dynamics and evaluation of efficacy of improved medico-social care program is needed.

Adult↗
PubMed2003

[Association between Bam HI RFLP p53 gene polymorphism and brain infarction volume in patients with atherothrombotic ischemic stroke].

A significant association between Bam HI RFLP p53 gene polymorphism and brain infarction volume in patients with atherothrombotic ischemic stroke (AIS) from Moscow population was found. Biallelic Bam HI polymorphism in exon 5 of p53 gene was studied in 96 AIS patients, 100 their healthy siblings, 41 patients with chronic ischemic disease and 42 their healthy siblings. Magnetic resonance tomography with morphometric program "Osiris" (the Hospital of the University, Geneva) for infarction volume measuring was used on day 1 and 7 after stroke onset. The patients with (-/-) p53 Bam HI genotype had the smaller brain infarction sizes (< 40 cm3), comparing to those with (-/+) (chi 2 = 19.7; p < 0.001) and (+/+) (chi 2 = 12.3; p < 0.001) genotypes. According to Bayes statistics, development of smaller infarction size in AIS may be predicted with over than 65% probability in the patients with (-/-) p53 Bam HI genotype. A significant association between p53 Bam HI polymorphism and infarction volume in patients with carotid atherothrombotic stroke confirms an important role of apoptosis in ischemic brain lesions formation that demands temporary antiapoptotic influence on patients with stroke.

Aged↗
PubMed2003

[Peculiarities of sporadic motor neuron disease associated with D90A and G12R mutations in Russian population].

Fifty-one blood samples of Russian patients with sporadic motor neuron disease were examined for mutations in Cu/Zn superoxide dismutase (SOD-1) gene. One female patient with amyotrophic lateral sclerosis (ALS) was heterozygous for G12R mutation. This patient suffered from ALS with cervical cord onset, pyramidal variant and fast progression. Mutation was also detected in her healthy son. Earlier, such mutation was described in 5 Italian patients with slow progressive ALS. Also, D90A SOD-1 gene associated haplotypes of the female ALS patients previously examined by the authors have been analyzed. A homozygous female patient with ALS was characterized by typical lumbar onset and extremely slow progression, as well as a female patient with heterozygous mutation and moderate progression carried so-called "Scandinavian" haplotype. To our knowledge, it is the first report on the finding of the haplotype considered as a "protective" one in the subjects heterozygous for D90A mutation with clinical symptoms of ALS. Mechanisms of "protective" influence of this haplotype on ALS course are not yet elucidated. Our finding suggests that the presence of "Scandinavian" haplotype does not completely protect from the disease development in patients exposed to other more pathogenic causative factors. This assumption is in line with modern conceptions on motor neuron disease as a complicated multifactor disorder.

Aged↗