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Biomedical subjects

V Eusebi

Publications and source records attributed to V Eusebi.

At least 163 records · Page 9Linked to original sources
PubMed1985

Amphicrine--composite calcitonin and mucin-producing--carcinoma of the thyroid.

A case of thyroid carcinoma in a 38-year-old male with a high serum level of calcitonin is reported. The tumor was composed of polygonal argyrophilic cells, signet ring Alcian blue-positive cells, and a minority of amphicrine elements. The same polymorphic cellular component was seen at the ultrastructural level. Anticalcitonin immunoperoxidase revealed that the majority of cells were positive, including the amphicrine cells. In spite of calcitonin production it is felt that cases similar to the present one should be distinguished from "ordinary medullary" carcinoma and the terms "amphicrine" or "composite calcitonin and mucin-producing carcinoma" are proposed.

Adult↗
PubMed1985

Small-cell (oat cell) carcinoma of the endometrium.

We report a case of small-cell (oat cell) carcinoma of the endometrium in a 59-year-old woman. The tumor was confined to a portion of an adenomatous polyp. It exhibited argyrophilia with Grimelius' stain and dense-core endocrine-type granules by electron microscopy. The patient was alive and well one year later. To our knowledge, this is the fourth reported case of a small-cell carcinoma of the endometrium exhibiting endocrine differentiation.

Carcinoma, Small Cell↗
PubMed1985

Chromogranin-reactive endocrine cells in argyrophilic carcinomas ("carcinoids") and normal tissue of the breast.

Breast carcinomas, either positive or negative with the Grimelius' silver procedure, benign fibroadenomas, duct papillomas, and areas of histologically normal breast tissue were tested immunocytochemically with the mouse monoclonal antibody LK2H10 directed against human chromogranin. This is regarded as a general stain for polypeptide-hormone-producing cells and tumors. In 3 of the 9 cases of argyrophilic carcinoma, but in none of 12 ductal infiltrating carcinomas, chromogranin-positive cells were found: the number of reactive cells was very low in 1 case, while in the other 2 carcinomas about 50% of the argyrophilic cells appeared stained. In areas of histologically normal breast tissue, rare argyrophilic chromogranin-positive cells were detected. This study is the first reported evidence concerning the presence of endocrinelike cells probably belonging to the diffuse neuroendocrine system in the normal mammary parenchyma. Our data are consistent with the endocrine nature of at least some of the breast argyrophilic carcinomas.

Adult↗
PubMed1984

Morphofunctional findings in adenocarcinoma of endometrium.

Ten cases of adenocarcinoma of the endometrium were studied using histochemical methods for mucopolysaccharides and immunocytochemical methods for the localization of various markers, such as EMA (epithelial membrane antigen), GCDFP-15 (gross cystic disease fluid protein) and CEA. Four cases appeared to contain High Iron Diamine-positive substance and 6 cases were positive using an immunocytochemical method with a polyclonal anti-CEA antiserum. In contrast, no cases stained when a monoclonal anti-CEA antiserum was employed. This suggested that HID- positive material could be a sign of differentiation towards cells with a secretory function.

Adenocarcinoma↗
PubMed1984

Spindle cell tumours of the skin of debatable origin. An immunocytochemical study.

Twelve cases of malignant spindle-cell and sarcomatoid tumours of the skin of debatable nature were studied by immunocytochemical methods, using four antisera which might help contribute to resolution of the problems. The initial diagnosis made on structural grounds was confirmed by immunocytochemistry in six of eight cases in which a specific diagnosis had been made (one melanoma, three squamous carcinomas and two atypical fibroxanthomas). One case, initially regarded as AFX was reclassified as a squamous carcinoma, while a further case of possible AFX could not be confirmed by immunocytochemical study. Of the four cases in which structural examination was inconclusive, two were identified as squamous carcinomas and one as a melanoma by virtue of tumour markers. The fourth case was an intriguing actin-rich tumour of uncertain nature. Immunocytochemistry, despite certain limitations, has a valuable role to play in the analysis of the problematic spindle-cell malignant and pseudomalignant tumours of the skin.

Actins↗
PubMed1984

Apocrine differentiation in lobular carcinoma of the breast: a morphologic, immunologic, and ultrastructural study.

The frequency of apocrine differentiation in breast carcinomas, assessed on purely morphologic grounds, is controversial. Apocrine differentiation in two cases of lobular carcinoma in situ (lobular neoplasia; LCIS) is reported for the first time. Using an immunohistochemical method for the detection of GCDFP-15, a protein present in apocrine epithelium and in the fluid of tension cysts of the breast, the apocrine differentiation in LCIS is confirmed. The histiocytoid variant of invasive lobular carcinoma is shown to be "apocrine" in nature, antigenically at least. The ultrastructural findings in one case of histiocytoid carcinoma are discussed in the context of an apparent discrepancy between the morphologic features of this tumor and the presence of an apocrine antigenic marker.

Adult↗
PubMed1984

Giant cell tumor of major salivary glands: report of three cases, one occurring in association with a malignant mixed tumor.

Three cases of a heterofore undescribed neoplasm of major salivary glands morphologically similar to giant cell tumor of bone are presented. All tumors were located in the parotid gland of adult individuals, and all patients are alive and well following surgical excision. One of the three cases was associated intimately with a malignant mixed tumor (carcinoma in pleomorphic adenoma). Ultrastructural and immunohistochemical studies failed to provide conclusive evidence about the specific nature of the tumor cells. The major salivary glands should be added to the long list of organs in which extraskeletal giant cell tumors have been observed, whether alone or in association with an epithelial malignancy.

Adenoma, Pleomorphic↗
PubMed1984

Immunohistochemical localization of myoglobin in nonmuscular cells.

Myoglobin has been detected by the immunoperoxidase technique in the cytoplasm of nonmuscular cells present in malignant tumors infiltrating skeletal muscle. The positive cells were reactive histiocytes in one case, and malignant cells (breast carcinoma, malignant melanoma, and malignant lymphoma) in the others. These findings indicate the need to exercise caution in the interpretation of myoglobin stain for the diagnosis of rhabdomyosarcoma under these circumstances.

Breast Neoplasms↗
PubMed1984

Primary oat cell carcinoma of the kidney.

A malignant renal neoplasm with all the morphologic attributes of oat cell (small cell, neuroendocrine) carcinoma is presented. It metastasized widely to regional lymph nodes and resulted in the death of the patient. Ultrastructurally, the tumor contained dense-core endocrine-type secretory granules. It had a cell component which was argyrophilic and which gave a positive immunocytochemical reaction for calcitonin. To the best of our knowledge, this is the first documentation of this tumor type in the kidney.

Aged↗
PubMed1984

Squamous cell carcinoma with sarcoma-like stroma of the nose and paranasal sinuses: report of two cases.

Two cases of squamous cell carcinoma with sarcoma-like stroma of the nose and paranasal sinuses are described. To the best of our knowledge, this is the first report of such a localization for this tumour type. Keratin was localized by immunocytochemistry in the epithelial cells, as well as in occasional stromal-like elements. The importance of distinguishing these tumours from morphologically similar neoplasms is emphasized in view of their different therapeutic approach.

Aged↗
PubMed1984

Neoplastic cells containing lysozyme in gastric carcinomas.

A case of gastric carcinoma mostly composed of cells with histological, immunohistochemical and ultrastructural features of Paneth cells prompted a comparative investigation of the occurrence of similar cells in gastric, colorectal and mammary carcinomas. Cells containing lysozyme were demonstrated by the immunoperoxidase-PAP technique in 34.9% of 83 gastric carcinomas. They were found in 38% of intestinal-type and in 30% of diffuse-type tumours. Paneth-type granules were demonstrated ultrastructurally in 4 of 7 carcinomas in which lysozyme had been demonstrated immunohistochemically. No lysozyme was demonstrated in a series of 30 breast carcinomas and in only 1 of 27 cases of colorectal neoplasm. The possibility of using lysozyme as a marker for some carcinomas of gastric origin is considered.

Adult↗
PubMed1984

Morpho-functional changes in normal cyclic endometrium.

Eight cases of normal endometrium in the early proliferative phase and an equal number of cases in the late secretive phase were stained histochemically for acidic mucosubstances and immunocytochemically for epithelial membrane antigen (EMA), carcinoembryogenetic antigen (CEA) and Gross Cyst Disease Fluid Protein -15 (GCDFP-15) production. Alcian blue positive material was observed in both phases of the cycle, although the distribution was different. Sulphated mucosubstances were only present in the secretive phase. No CEA nor GCDFP-15 immunoreactive material was detected. EMA positive staining was observed in all cases, but the distribution varied according to the two different phases of the cycle.

Adult↗
PubMed1984

Small-cell osteosarcoma of the mandible. Case report.

A case of small-cell osteosarcoma of the mandible in an 8-year-old girl is reported. This unusual variant may simulate Ewing's sarcoma and other small-cell neoplasms affecting jaws in children. The correct recognition of this type of tumor may be important for an appropriate choice of treatment.

Child↗
PubMed1983

Strumal carcinoid of the ovary: an immunohistochemical and electron microscopic study.

A case of strumal carcinoid of the ovary is reported. The follicles contained T4-immunoreactive substance, whereas the carcinoid component of the tumor had a trabecular structure and showed argyrophilic elements. Pancreatic-polypeptide and enteroglucagon were localized in these cells by an immunocytochemical method. The same cells displayed small electron-dense endocrine-like granules. A common precursor for the thyroid and carcinoid components is postulated.

Carcinoid Tumor↗
PubMed1983

Immunohistochemical analysis of benign and malignant papillary lesions of the breast.

The histocytological diagnostic criteria and recently developed immunohistochemical procedures selective for either the epithelial or the myoepithelial mammary cells have been tested in a series of 60 cases of papillary lesions of the breast. These included 15 benign solitary intraductal papillomas, 41 papillary carcinomas (29 pure and 12 associated with other types of in situ or invasive ductal carcinoma), and four cases of "suspected" papillary carcinomas. Markers for epithelial cells (EMA) and for apocrine metaplasia (GCDFP-15) did not permit a distinction between benign and malignant papillary lesions; however, immunocytochemical staining for CEA using monoclonal antibodies, and for actin (a marker of the myoepithelial cells) was discriminative in this respect. Benign papillomas have a basal layer of actin-rich myoepithelial cells; the cytoplasm of the epithelial cells is CEA negative. Papillary carcinomas lack the myoepithelial layer, except in areas where multiple papillomas are present, associated with ductal or papillary cancer. CEA was detected in 85% of carcinomas. Two of the cases of "suspected carcinoma" lacked myoepithelial cells and were interpreted as carcinomas. It is concluded that the immunocytochemical methods for cell markers can offer valuable data in the study and diagnosis of papillary lesions of the breast; it is difficult, however, to be categorical in borderline cases since in our experience, the behavior of the malignant papillary lesions of the breast is usually favorable. Residual foci of multiple intraductal papillomas were found in seven cases of papillary carcinoma, supporting the pre-neoplastic potential of this condition.

Actins↗
PubMed1983

Merkel cell carcinoma of the skin: the structure and origin of normal Merkel cells.

A series of 15 Merkel cell tumours of skin is reported. They occur dominantly on the head and neck and on the extremities of elderly women, frequently presenting as a reddish nodule. Three cases were associated with squamous carcinoma at the same site, an association deserving further study. There are two main patterns: the commoner one takes the form of a trabecular carcinoma in the dermis mimicking metastatic carcinoma, including oat-cell carcinoma and neuroblastoma: a dissociated-cell form mimicks malignant lymphoma. The triad of vesicular nuclei with very small nucleoli, abundant mitotic activity and apoptosis is so characteristic as to be virtually pathognomonic in conjunction with structural features. Argyrophilia is common, but Bouin fixation is necessary to demonstrate it regularly. Small round secretory granules (89 +/- 18 nm) with narrow haloes, and an abundance of intermediate size filaments are among the ultrastructural hallmarks. There is a close similarity between better differentiated tumour cells and normal Merkel cells. The neural crest origin of MC is in doubt both on the basis of studies of the development and regeneration of MC and from the study of Merkel cell tumours.

Adenocarcinoma↗