Search PubMed⌕ Search

Biomedical subjects

V Eusebi

Publications and source records attributed to V Eusebi.

At least 145 records · Page 8Linked to original sources
PubMed1988

Extracardiac rhabdomyoma: An immunocytochemical study and review of the literature.

Two cases of 'adult' rhabdomyoma and 2 of 'fetal' rhabdomyoma have been immunologically studied using several antisera specific of skeletal muscle fibers proteins. This paper also deals with a review of the literature on these rare benign skeletal muscle proliferations. It is concluded that 'adult' rhabdomyoma contains fetal myosin and therefore the term 'adult' does not seem immunologically appropriate any longer. In addition the tumoral cells show the same level of differentiation as seen in neonatal skeletal muscle. Therefore it seems that the definition of neonatal rhabdomyoma is more appropriate for these benign muscular proliferations. The 2 cases of fetal rhabdomyoma appear phenotypically similar to normally developing myoblasts and contain fetal myosin. Therefore it seems that the definition 'fetal' is here appropriate for what appears to be a disorganized proliferation of otherwise normal fetal skeletal muscle fibers. The differential diagnosis between these latter tumors and rhabdomyosarcomas has been discussed.

Adult↗
PubMed1988

Trabecular carcinoid of the skin with cellular stroma.

A case of trabecular carcinoid of the skin of an 80-year-old man is described. This tumor's structure is totally different from that of trabecular carcinomas as described by Toker and Tang and Toker. It is formed by long, irregularly branching, serpiginous trabeculae, which are Grimelius positive and chromogranin immunoreactive. This appears to be the first case of trabecular carcinoid reported in the skin. An adnexal origin for this tumor is suggested.

Aged↗
PubMed1987

Inflammatory pseudotumor of the liver: report of two cases.

Inflammatory pseudotumor of the liver is a rare benign lesion which clinically and grossly is often interpreted as a neoplastic condition. It usually arises in children or young adults. Only 11 cases have been described in the literature, and its etiology is still uncertain. Two new cases of inflammatory pseudotumor in adult male patients are reported here. Morphologically, these cases summarize all the features seen in the other cases described previously. They showed fibrous bands delimiting microabscesses made up of granulocytes, mononuclear elements and foamy macrophages. These lesions have in common the symptomatology which is amenable to infectious disease. The purpose of this paper is to draw attention to the existence of a lesion that clinically simulates a neoplastic condition of the liver. This lesion should be histologically recognized to avoid excessive surgical treatment.

Aged↗
PubMed1987

Adenomyoepithelioma of the breast with a distinctive type of apocrine adenosis.

A newly recognized type of dimorphic carcinoma of breast, distinct from adenoid cystic carcinoma, is described. It is characterized by a predominantly solid, clear cell myoepithelial proliferation, with centrally situated glandular lumina lined by apocrine cells. All cases arose in association with a distinctive type of atypical apocrine adenosis which has to be distinguished from microglandular adenosis and from tubular carcinoma. The biological behaviour of the tumour remains to be ascertained on the basis of longer follow-up, but it appears to have only limited malignant potential.

Actins↗
PubMed1987

The interest of actin immunocytochemistry in diagnostic histopathology.

The immunohistochemical detection of different isoforms of actin provides useful data in the histopathological diagnosis of human tumours. The marked resistance of this protein to fixation and embedding procedures makes it a practical alternative to the ultrastructural search of microfilaments in routine tissue sections. Staining with monoclonal antibodies against alpha smooth muscle actin is helpful in the diagnosis of benign and malignant smooth muscle tumours and allows the differentiation of various types of spindle cell sarcomas. These antibodies are also useful in tracing the myoepithelial cells in normal and various pathological conditions of the breast and salivary glands. Skeletal muscle actin is a reliable marker in the diagnosis of rhabdomyosarcomas. Its use should be combined and complemented with other markers (i.e. desmin; foetal, slow and fast myosins; myoglobin) to monitor the degree of rhabdomyoblastic differentiation of the neoplastic cells in single cases, a parameter of potential prognostic value.

Actins↗
PubMed1987

DNA cytofluorometry in micro-dissected paraffin embedded breast tissue: description of a method.

DNA microfluorometry on smears obtained from paraffin embedded tissue has been shown to be a distinctive possibility. In this paper a simple method for the detachment of cells from mammary ducts and ductules is described. Areas of interest were selected in conventional slides stained with Haematoxylin and Eosin. The corresponding paraffin embedded block was then dewaxed. The areas under study were retraced with a stereomicroscope and the cells within ducts and ductules were scraped out with a 0.4 mm diameter fine needle. Cells were isolated with mechanical and enzymatic procedures and stained with the Feulgen reaction. The DNA content of single cells was then measured using a microfluorometer.

Breast Neoplasms↗
PubMed1986

Ultrastructural organization of nucleoli in benign naevi and malignant melanomas.

We have studied the ultrastructural organization of nucleoli in benign naevi and malignant melanomas. In benign naevus cells the nucleoli displayed a compact ribonucleoprotein distribution, with one or two large fibrillar centres. In malignant melanoma cells the nucleoli were large with an irregular, nucleolonema-like ribonucleoprotein distribution and they exhibited numerous, small fibrillar centres. Statistical evaluation of the size of fibrillar centres indicated a mean value of 0.482 micron 2 +/- 0.136 SD for naevi and 0.221 micron 2 +/- 0.128 SD for malignant melanomas. These features, together with the more dispersed chromatin pattern of malignant melanoma nuclei compared with those of benign naevus cells, are proposed as diagnostic parameters which differentiate benign naevi from malignant melanomas at the ultrastructural level.

Cell Nucleolus↗
PubMed1986

RMZ: a new cell line from a human alveolar rhabdomyosarcoma. In vitro expression of embryonic myosin.

The RMZ cell line was established from a bone marrow metastasis of a human alveolar rhabdomyosarcoma. Since the beginning of the in vitro culture, RMZ cells showed differentiation-related morphological heterogeneity: actively proliferating polygonal or spindle-shaped cells were observed along with a few multinucleated myotube-like structures and giant cells, frequently multinucleated. All these cell types were still present after over 40 passages. A set of clonal derivatives has been obtained from the second in vitro subculture. All the clones showed the same morphological heterogeneity of the parental cells, but differed from one another in the degree of differentiation. Multinucleated myotube-like structures were strongly stained by anti-desmin antibody; most mononuclear cells were weakly stained. About 80% of RMZ and cloned cells were scored as desmin-positive in cytocentrifuged preparations. The expression of embryonic myosin heavy chain, specifically recognized by the monoclonal antibody BF-G6, was found in RMZ cell line and was localised in the myotube-like structures. Only a few giant cells and rare mononucleated polygonal cells were stained. The average proportion of BF-G6 positive cells in cytocentrifuged preparations was of about 6% of the total RMZ cells. In the two RMZ clones studied, the expression of embryonic myosin was correlated to the proportion of myotube-like structures: a BF-G6 positivity of 35% was found in the most differentiated one.

Cell Count↗
PubMed1986

Fetal heavy chain skeletal myosin. An oncofetal antigen expressed by rhabdomyosarcoma.

Fetal heavy chain skeletal myosin is normally present in fetal skeletal muscle. The study of 21 cases of rhabdomyosarcoma using specific antisera for fetal myosin, as well as for slow myosin, myoglobin, and desmin, led to positive findings in all cases with at least one antiserum. Desmin was localized in all cases and fetal myosin in 17 cases (81%), while myoglobin and slow myosin were present in 11 and eight cases, respectively. The localization of fetal myosin in rhabdomyosarcoma indicates that it is a type of oncofetal antigen. Because fetal myosin is found in small rhabdomyoblasts, it can be a useful marker in cases that usually constitute diagnostic problems.

Adult↗
PubMed1986

Acantholytic variant of squamous-cell carcinoma of the breast.

Three cases of acantholytic squamous-cell carcinoma of the breast are reported. They all had histological features resembling those of angiosarcoma or adenocarcinoma. They were not angiosarcoma, since in all three cases areas of squamous differentiation were present; in addition, the neoplastic cells were negative when stained for factor VIII, but were positive with anti-epidermal keratin. The glandular pattern exhibited, especially in Case 2, was difficult to differentiate from that of an ordinary carcinoma. However, the presence of dyskeratotic cells within the lumina, and the absence of alcian blue/periodic acid-Schiff positive material, and epithelial membrane antigen staining, were evidence against the diagnosis of adenocarcinoma. The patients died 5, 6, and 16 months after the diagnosis. Tumors with these histological features may have a very aggressive clinical course.

Aged↗
PubMed1986

Fast and slow myosins as specific markers of muscle: an immunocytochemical study.

Slow and fast myosins are specific markers of skeletal muscle. The specificity of anti-fast and anti-slow myosin antisera been verified on frozen and paraffin embedded tissues. The optimal fixative useful for routine histopathological purposes has been investigated. It is suggested that these two antisera can be used in the diagnosis of rhabdomyoblastic tumours substituting for or complementary to myoglobin, a well known marker of striated muscle.

Animals↗
PubMed1986

Cellular neurothekeoma.

The histological and immunohistochemical features of 3 cases of cellular neurothekeomas of skin are presented. Based on the findings in these cases and comparisons made with others reported in the literature, it is proposed that neurothekeomas may be divided into two distinct types: the myxoid and cellular variants. The relationship between these variants and a related entity, the Pacinian neurofibroma, is discussed.

Female↗
PubMed1986

Immunocytochemistry of rhabdomyosarcoma. The use of four different markers.

Fast myosin and slow myosin are specific markers of skeletal muscle, in addition to myoglobin. This study of 15 specimens of rhabdomyosarcomas from 13 patients using specific antisera for the three markers as well as for desmin led to positive findings in all cases with at least one antiserum. Desmin was present in all cases; fast myosin and myoglobin were present in 10 cases each. Slow myosin was present in six cases. It appears that the combination of several markers is helpful in differentiating rhabdomyosarcomas from other tumors. The markers considered were generally more abundant in neoplastic elements with large amounts of cytoplasm. This finding suggests that the larger cells of rhabdomyosarcomas are more differentiated than smaller rhabdomyoblasts, which were often negative with some of the antisera used.

Adolescent↗
PubMed1986

Apocrine carcinoma of the breast. A morphologic and immunocytochemical study.

The mode of recognition and hence the frequency of apocrine differentiation in breast carcinomas, assessed on purely morphologic grounds, remains uncertain. One hundred consecutive cases of breast carcinoma were studied in order to establish the incidence of this type of tumor. With the use of an immunocytochemical method for the detection of GCDFP-15, a protein present in apocrine epithelium and in the fluid of tension cyst of the breast, the presence of apocrine differentiation was confirmed in 4 cases initially diagnosed as apocrine carcinomas on histologic grounds. Eight additional cases contained immunoreactive cells: 1 contained 10% of positive cells scattered throughout the tumor, and the other 7 cases were only focally positive. In 4 of these latter cases positive staining was confined to the in situ component. The ultrastructural findings in 2 cases of apocrine carcinoma are discussed in order to link the morphologic features for recognizing this tumor type and the presence of the antigenic apocrine marker.

Adult↗