The use of beta-galactosidase as a tracer in immunocytochemistry.
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Animals↗
Biomedical subjects
V Eusebi
Publications and source records attributed to V Eusebi.
'Carcinoid' tumours of the breast: the morphological spectrum of argyrophil carcinomas.
Fourteen 'carcinoid' tumours of the breast are described. They are separable into five with and nine without intracellular mucin. All the tumours are argyrophil, but none is argentaffin. Four tumours studied ultrastructurally contain dense-core granules. Argyrophil carcinomas represent the endocrine analogues of ductal carcinoma in situ, of invasive ductal carcinoma and probably of lobular carcinoma also. Current views vary between the one that the so-called carcinoid is a rare and totally distinct entity to the view, at the other extreme, that it is a very common variant of conventional breast cancer. On the basis of our findings, an intermediate view is justified: argyrophil carcinomas constitute about 5% of breast carcinomas and some varieties at least have non-argyrophil analogues. Factors influencing the prognosis in individual cases are discussed. Argyrophil carcinomas of the breast form a tumour spectrum with a wide range of morphological and histochemical appearances and a variable prognosis.
Aged↗
Primary endocrine carcinoma of the parotid salivary gland associated with a lung carcinoid: a possible new association.
An endocrine carcinoma of the left parotid salivary gland in a 58-year-old woman is reported. The tumour displayed a large argyrophilic cell-component and at ultrastructural level endocrine-like granules (ELG) were evident. As endocrine-paracrine cells are not normally present in the parotid, it is suggested that the endocrine elements may have been derived from an anomalous differentiation of the ductal epithelial stem cells. A bronchial carcinoid, removed seven years previously, proved structurally, cytologically and histochemically different from the tumour of the parotid salivary gland. It is proposed that the occurrence of the two tumours might be an as yet undescribed association which is more than fortuitous.
Carcinoid Tumor↗
Endocrine-paracrine cells in pancreatic exocrine carcinomas.
Eleven cases of primary pancreatic adenocarcinomas have been investigated histochemically, immunohistochemically and with electron-microscopy. Endocrine-paracrine (EP) cells were present in six of these tumours. In one case numerous 5HT-enterochromaffin cells (EC) of the intestinal type and a few somatostatin immunoreactive D cells were found. Two cases contained insulin-immunoreactive cells and another case displayed glucagon-IR elements. In the remaining two cases argyrophilic cells were present. These findings demonstrate that polypeptide hormone or amine production is not restricted to islet cell tumours. It is suggested that both endocrine and exocrine components of the tumours studied might have derived from a common precursor.
Adenocarcinoma↗
A two-stage immunocytochemical method for oestrogen receptor analysis: correlation with morphological parameters of breast carcinomas.
An immunocytochemical method for oestrogen receptor (ER) analysis of breast carcinomas is described and compared with the dextran-coated charcoal (DCC) assay and a cytochemical method. The method described is superior to the purely cytochemical, especially in terms of sensitivity, demonstration of nuclear receptors and correlation with the DCC assay. The DCC assay is reliable except in the case of poorly cellular tumours: in these the immunocytochemical method is far superior, being independent of tumor cellularity. With this new method, lobular carcinomas are shown to be ER-rich compared with their ductal counterparts, a conclusion currently disputed, largely because methods employed mostly fail to take account of tumour cellularity. A combination of the DCC assay and the immunocytochemical method here described gives the maximum information about the ER-status of a particular carcinoma.
Adult↗
Benign smooth muscle cell metaplasia in breast.
Three different benign breast lesions showing smooth muscle cells in the stroma are reported. Benign smooth muscle cells are rarely found in the breast, and it is likely that they originate from metaplastic changes of the stroma itself.
Adenofibroma↗
Masson's intravascular vegetant hemangioendothelioma.
Eleven cases of Masson's hemangioendothelioma are reported. This benign condition must be distinguished histologically from numerous malignant endothelial lesions. Two different papillary structures were observed; one defined as "inflammatory papilla" was constantly associated with a thrombus; the other, defined as "fibrous papilla" was observed in 4 cases where no thrombus could be found. It is postulated that the inflammatory papillae are an exuberant endothelial growth during the normal organisation of a thrombus; fibrous papillae, it is suggested, might be reactive to blood or lymph stasis.
Adolescent↗
Non-encapsulated sclerosing lesion of the breast.
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Breast Diseases↗
Lobular endocrine neoplasia in fibroadenoma of the breast.
Four cases of endocrine neoplasia within fibroadenomas of the breast are described. The tumours are identifiable by light microscopic, silver impregnation and ultrastructural studies. Argyrophilia and the presence of dense-core granules establish the identity of this tumour type. The differential diagnosis from, and possible relation to, lobular carcinoma in situ are discussed.
Adenofibroma↗
Endocrine differentiation in mucoid carcinoma of the breast.
Mucoid carcinoma of the breast is not a single homogeneous entity. It comprises two main variants separable on structural and cytological grounds and a smaller transitional type. The salient pathological differences are detailed. One variant is usually arygrophilic and contains dense core granules of the type seen in endocrine tumours. Both endocrine and amphicrine cells have been identified in this sub-type. The relationship of this endocrine variant of mucoid carcinoma to other argryophil carcinomas (so-called 'carcinoids') of the breast is discussed.
Adenocarcinoma, Mucinous↗
Immunoperoxidase method for detection of immunoglobulins.
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Immunoenzyme Techniques↗
DNA microspectrophotometric study in smear preparations from paraffin embedded tissue.
A DNA microspectrofluorimetric study on smears from cells detached from paraffin embedded tissue is presented. The cellular DNA values of these smears were compared with the cellular DNA values of fresh imprints and paraffin embedded sections obtained from the same tissue. The histograms were similar for fresh imprints and paraffin embedded smears while the DNA values in the sections were notably different. This method appears very suitable for retrospective studies of DNA using microspectrofluorimetry in routine histopathology.
DNA↗
Endocrine differentiation in basal cell carcinoma.
Argyrophilic cells have been found in 2 different basal cell carcinomas (BCC), and membrane-bound endocrine-like granules, in the carcinoid range, have been observed in 4 consecutive cases of such tumors. It is postulated that BCC can occasionally display cells showing an endocrine differentiation.
Aged↗
Tubular carcinoma: a variant of secretory breast carcinoma.
Fifteen cases of tubular carcinoma of the breast have been studied using histochemical methods for mucosubstances, immunocytochemical methods for casein and actin and conventional electron microscopy. Mucosubstances and casein were demonstrated lying freely in the lumina of the tubules. Occasionally, mucosubstances and casein assumed the form of target-like intracytoplasmic 'inclusions' like those characteristically seen in lobular carcinoma. The neoplastic cells did not react with antisera specific against actin. Even at ultrastructural level no myoepithelial cells were observed, whilst villi were revealed along the tubular luminal surface. It appears that, in addition to distinctive biological, histological and ultrastructural features, tubular carcinoma has an almost constant histochemical pattern. This suggests a differentiation towards epithelial secretory cells engaged in intensive milk protein production which has also been shown to be a feature of lobular carcinoma. It is concluded that though lobular carcinoma and tubular carcinoma of the breast have been traditionally regarded as two distinct entities, they have certain similar functional characteristics and it is postulated that these two tumours could represent the extreme variants fo the same entity: the infiltrative lobular carcinoma being the most undifferentiated and tubular carcinoma the most highly differentiated.
Actins↗
[Extra-lymphnodal pseudolymphoma].
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Diagnosis, Differential↗
Primary oat-cell carcinoma of the larynx.
A primary oat-cell carcinoma of the larynx in a 63-year-old man is reported. The oat-cell pattern appeared intermingled at one edge with a squamous carcinoma. The origin of this mixed tumour is considered together with the literature of extrapulmonary carcinomas having an oat-cell pattern.
Carcinoma, Small Cell↗
Yellow-brown spindle bodies in mesenteric lymph nodes: a possible relationship with melanosis coli.
Four cases are described in which spindle-shaped, yellow-brown bodies were seen in the mesenteric lymph nodes of patients with melanosis coli. A comparison of the staining reactions and ultrastructural appearances of the spindle bodies and melanosis pigment suggest that they are related within the broad group of lipofuscins and that the spindle bodies are formed as a result of coalescence of lysosomes containing the pigment.
Adenocarcinoma↗
Melanocyte colonization and pigmentation of breast carcinoma.
Colonization of mammary cancers by melanocytes with attendant pigmentation of cancer cells by melanin occurs in most cases of breast cancer in which the dermal--epidermal interface is reached by the tumour cells. There is evidence to suggest that the mammary cancer may become colonized at the expense of the epidermis, which is focally depleted of melanocytes. Melanocytes can even be found in groups of tumour cells within lymphatics. Colonization and pigmentation of breast cancer is usually only easily detected by selective staining methods. In very rare cases it can be gross and easily detectable in routine stains. The implications of this migratory phenomenon are discussed in terms of the biology of the melanocyte.
Breast Neoplasms↗