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Biomedical subjects

T Usui

Publications and source records attributed to T Usui.

At least 487 records · Page 27Linked to original sources
PubMed1984

Free amino acid concentrations in plasma, erythrocytes, granulocytes, and lymphocytes in umbilical cord blood, children, and adults.

Concentrations of free amino acids were measured concurrently in plasma, erythrocytes, granulocytes, and lymphocytes in umbilical cord blood, neonates, children, and adults. In each age group, the patterns of free amino acids were fairly similar in plasma and erythrocytes except for aspartic acid which was more abundant in erythrocytes. Of the amino acids in granulocytes, 71-77% was taurine; in lymphocytes taurine, aspartic acid, and glutamic acid comprised 35-44%, 18-24%, and 20-28%, respectively, of the total in all age groups. Leukocytes may contribute to the interorgan transport of amino acids to about 10% of the erythrocytes' contribution. Postnatally, the levels of glutamic acid and tyrosine in plasma; threonine plus glutamine, serine plus asparagine, and tyrosine in erythrocytes; histidine in granulocytes; and glutamic acid in lymphocytes were significantly increased (p less than 0.001); while the levels of phenylalanine and lysine in plasma; taurine in erythrocytes; valine and phenylalanine in granulocytes; and threonine plus glutamine, tyrosine, and phenylalanine in lymphocytes were significantly decreased (p less than 0.001). After the neonatal period concentrations of taurine and aspartic acid in erythrocytes, taurine and valine in granulocytes, and tyrosine and phenylalanine in lymphocytes increased gradually with age; while concentrations aspartic acid in plasma, histidine in granulocytes, and glycine in lymphocytes decreased gradually with age. The levels of glycine and valine in plasma, alanine and valine in erythrocytes, serine plus asparagine, glycine, alanine, and tyrosine in granulocytes, and aspartic acid, serine plus asparagine, and alanine in lymphocytes remained constant in all age groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1984

Superoxide dismutase activity in leukemic blasts of children with acute leukemia.

Superoxide dismutase activity was determined in leukemic blasts of 24 patients with acute leukemia and was correlated to its type. The activity was significantly lower in five patients with T cell or B cell leukemia, and there was a wide range of distribution in 15 patients with non-T, non-B cell leukemia. Manganese superoxide dismutase activity was significantly lower in all types of acute lymphoblastic leukemia than in normal mononuclear leukocytes. Both total and manganese superoxide dismutase activities in acute myeloblastic leukemia, however, were significantly higher than those in normal polymorphonuclear leukocytes. The prognosis in patients with a low superoxide dismutase activity in leukemic blasts seemed to be poorer. Determination of superoxide dismutase activity in leukemic blasts may have a prognostic value in the management of children with acute leukemia.

Adolescent↗
PubMed1984

Decreased superoxide dismutase activity of erythrocytes and leukocytes in Fanconi's anemia.

Superoxide dismutase (SOD) activity was determined in the erythrocytes and leukocytes of 5 patients with Fanconi's anemia (FA) and 1 with atypical Fanconi's anemia without any hematological disorder. SOD activity was decreased in the blood cells of the patients with Fanconi's anemia, but was normal in the atypical patient. The influence of SOD on the chromosome aberrations and hematological disorder in FA is discussed.

Anemia, Aplastic↗
PubMed1984

Presumed BCG infection in a boy with chronic granulomatous disease. A report of a case and a review of the literature.

A male child with chronic granulomatous disease (CGD) developed protracted axillary lymphadenopathy with liquefaction following a bacille Calmettle-Guérin (BCG) immunization. Except for phagocytic dysfunctions characteristic of the underlying disease, immunological examinations were normal. The literature dealing with CGD cases with disseminated BCG infection was reviewed. It is concluded that the possibility of CGD should be considered in those who developed such adverse reactions to BCG immunization.

BCG Vaccine↗
PubMed1984

Effect of zinc ion on human chorionic gonadotropin-stimulated in vitro production of cyclic AMP and testosterone by rat testis.

This study explores the effects of several divalent metal ions on the in vitro production of cyclic AMP, cyclic GMP, and testosterone by rat testicular tissue and on the amount of binding of [125I]human chorionic gonadotropin (hCG) in the testis. Zn2+ at concentrations of 10(-6) to 10(-4) M enhanced the hCG-stimulated production of cyclic AMP and testosterone, but only in the presence of Ca2+ X [125I]hCG binding to rat testicular tissue was not affected by Zn2+ X Cu2+, Ni2+, Co2+, and Mn2+ did not increase cyclic AMP or testosterone production in concentrations of 10(-7) to 10(-3) M and even inhibited them at a high concentration (10(-2) M). Cyclic GMP production was not affected by these divalent ions. These results suggest that Zn2+ may play an important role in rat testicular steroidogenesis.

Animals↗
PubMed1984

Radioimmunoassay of diethylstilbestrol in plasma of patients with prostatic carcinoma.

A method for the radioimmunoassay of diethylstilbestrol (DES) was established and it was used to measure the plasma concentration of DES in patients with prostatic carcinoma receiving intravenous drip infusion of 500 mg of diethylstilbestrol diphosphate (DES-DP). The sensitivity of this assay system was 40 pg per tube, with a recovery of 98%. The cross reactivity of the anti-DES antibody with estradiol, testosterone, and ethynylestradiol was less than 0.01% of DES, and that with DES-DP was 0.7%. In patients with prostatic carcinoma, plasma DES concentration at 6 and 24 hr after was declined to 10.2% and 4.4% of the level at the termination of intravenous administration of DES-DP, respectively; however, the concentration in patients with renal dysfunction was higher than that in ones with normal renal function.

Diethylstilbestrol↗
PubMed1984

The mitogenic effect of the lymphocytosis promoting factor from Bordetella pertussis on human T gamma and non-T gamma cells.

We studied the effect of lymphocytosis promoting factor (LPF), derived from the supernatant fluid of a culture of phase I Bordetella pertussis strain Tohama, on human lymphocyte proliferation. LPF was a potent mitogen for human mononuclear cells, specifically T cells. LPF failed to induce cytoplasmic immunoglobulin production by B cells. Removal of the monocytes from the T cell fraction diminished responses to LPF, but the response could be restored completely by the addition of 5.0% monocytes. These results suggest that LPF-induced cell proliferation is at least partially dependent on monocytes. In contrast to PHA, LPF stimulated T gamma cells to a greater extent than non-T gamma cells, but the magnitude of the T gamma or non-T gamma cell response was less than that of T cells, indicating that synergistic interactions between T gamma and non-T gamma cells are required for maximal response.

Bacterial Toxins↗
PubMed1984

Comparison of phorbol myristate acetate and phytohaemagglutinin as stimulators of in vitro T lymphocyte colony formation of human peripheral blood lymphocytes. I. Surface markers of colony cells.

Human T lymphocyte colonies were grown in methylcellulose semi-solid cultures in the presence of phytohaemagglutinin (PHA) and/or phorbol myristate acetate (PMA). Surface marker analysis showed lower percentages of OKT3- and OKT4-positive cells in PMA-induced colonies than those in PHA-induced colonies. The percentage of OKIa1-positive cells in PMA-induced colonies was approximately twice that in PHA-induced colonies. The percentage of OKT9-positive cells in PMA-induced colonies was significantly lower than that in PHA-induced colonies. These data suggest that the subsets of PMA-induced colony cells express a more immature phenotype than that of PHA-induced colony cells and that, among PMA-induced colony cells, there are fewer T cells in the proliferative status at the time tested. When 3 X 10(5)/ml monocyte-depleted T cells, at which concentration of seeded cells neither PHA nor PMA could induce colony growth, were cultured in the presence of both PHA and PMA, T cell colony growth was observed. In T cell colonies induced by a combination of PHA and PMA, the percentages of OKT3-, OKT4- and OKT8-positive cells were different from those in colonies induced by either PHA or PMA alone. These results suggest that PMA acts not only as a substitute for monocytes and/or interleukin-1, but may directly affect lymphocyte proliferation induced by a combination of PHA and PMA.

Antibodies, Monoclonal↗
PubMed1984

Vitamin D metabolism in osteogenesis imperfecta during calcitonin therapy.

The effect of calcitonin on plasma concentrations of 25-hydroxyvitamin D (25-OHD) and 1,25-dihydroxyvitamin D [1,25-(OH)2D] was studied in six patients with osteogenesis imperfecta. The mean pretreatment value of plasma 1,25-(OH)2D was significantly higher than the mean value of age-matched control subjects (P less than .05). High or normal plasma levels of 1,25-(OH)2D before calcitonin therapy were decreased after 1 month of therapy and remained normal thereafter in all six patients. Plasma 25-hydroxyvitamin D concentrations, which were normal before calcitonin injection, remained normal during calcitonin administration. These results indicate that there may be acute and chronic effects of calcitonin on vitamin D metabolism.

Adolescent↗