Synthesis of 4-hydroxy-2-alkenals and 4-oxo-2-alkenols from 4-tetrahydropyranyloxy-2-butenal and their antimutagenic effect against UV-induced Escherichia coli WP2 B/r Trp-.
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Aldehydes↗
Biomedical subjects
T Uno
Publications and source records attributed to T Uno.
Direct evidence that the cuticle surface of Trichinella spiralis muscle larvae shares antigenicity with stichocyte alpha-granules and the esophagus-occupying substance.
Antibodies against the cuticle surface of Trichinella spiralis muscle larvae were purified by means of immunoaffinity chromatography and incubated with ultrathin sections of muscle larvae. Major constituents of the parasite reactive with the purified antibodies included the cuticle surface, stichocyte alpha-granules, and the esophagus occupying substance of the muscle larvae. Thus the present data suggest that the cuticle surface is an antigenically different entity from the cuticle inner layers and its origin is likely stichocyte alpha-granules.
Animals↗
[Blood flow in the operating field during controlled hypotension by nitroglycerin, trimetaphan and prostaglandin E1].
We used the laser flowmeter to measure and compare the effect of nitroglycerin (TNG), trimetaphan (TMP) and prostaglandin E1 (PGE1) on the blood flow of operating field during hypotension induced by each drug. The study was done in 33 patients (TNG:13, TMP:13 and PGE1:7) anesthetized with modified NLA who underwent radical mastectomy. We put the probe of the laser flowmeter on the skin of the opposite breast to the operating field and measured the skin blood flow change. Measurements were made before and during hypotension induced by each drug. TMP-induced hypotension decreased blood flow significantly (P less than 0.05), but TNG and PGE1 had no significant effect. Intraoperative blood losses of TNG and PGE1 group were not significantly larger than that of TMP group. From this study we conclude that TNG, TMP and PGE1 can dry the operating field and decrease intraoperative blood loss similarly. Therefore we should choose the drugs to induce hypotension considering the effect of the drug on blood flow of the important organs and each patient's complications.
Adult↗
Immunocytochemical evaluation of Trichinella spiralis larval antigen.
The antigenicity of Trichinella spiralis muscle larvae was studied for the purpose of developing an immunodiagnostic test for trichinosis. Ultrathin sections of fixed larvae were treated with sera from patients with a variety of parasitic infections including trichinosis, anisakiasis, gnathostomiasis, fascioliasis, dirofilariasis, severe trichuriasis, moderate trichuriasis, paragonimiasis, cysticercosis cellulosae hominis and diphyllobothriasis, and then treated with protein A-tagged colloidal gold. The cuticle surface and stichocyte alpha 0- and alpha 1-granules reacted predominantly with sera from trichinosis and severe trichuriasis patients, and negligibly with the others. By contrast, the cuticle inner layer, hypodermis, and hemolymph reacted with all sera examined though there was some variability in the intensity of the staining. These results suggest that the cuticle surface and stichocyte alpha-granules possess a high degree of specificity for antibody-produced in T. spiralis infection, whereas the other larval components exhibit nonspecificity.
Animals↗
A spectrum of antibody response with time after Trichinella spiralis infection in rats.
A chronology of class-specific antibody response against the Trichinella spiralis infection in Fischer rats was investigated. G-class antibodies against the cuticle inner layer(s), hypodermis, hemolymph, glycogen aggregates, discrete areas in genital primordial cells, intestinal gland cell granules, and cytoplasmic granules in the cords were detectable 2 wk after infection (the rapid-responding group), whereas G-class antibodies against the cuticle surface, stichocyte granules, and the esophagus-occupying substance were detected 6 wk after infection (the slow-responding group). M-class antibodies recognized a narrower spectrum of antigens than did G-class antibodies; M-class antibodies against hemolymph, cord granules, and intestinal gland cell granules were not detectable. M-class antibodies tended to decrease in titer with time after infection. This tendency was more striking with antibodies against the rapid-responding group than with those against the slow-responding group. This information sheds light upon antibody response against many antigenic components of T. spiralis muscle larvae.
Animals↗
The intestinal gland of Trichinella spiralis with emphasis on morphology and antigenicity.
The intestinal gland of muscle larvae and adult worms of Trichinella spiralis was investigated with emphasis on its morphology and antigenicity. The gland is situated at the junction between the stichosome and the ampullar portion of the midgut. The cytoplasm was characterized by the presence of cytoplasmic granules. The granules, measuring 1 micron at maximum, were round or sometimes irregular in shape, and of homogeneous appearance with medium to high electron density. Rough endoplasmic reticulum was not prominent. Glycogen granules were absent. The gland was surrounded externally by the basal lamina and hemolymph. The cell membrane was extensively invaginated, and coated pits and vesicles were often observed. The cytoplasm was rather eosinophilic, PAS-negative, and stained red or yellow by AZAN. The cytoplasmic granules were antigenic against trichinosis sera from humans and rats, and cross-reacted with sera from paragonimiasis, trichuriasis and gnathostomiasis patients.
Animals↗
Ultrastructural localization of antigenic substances in Trichinella spiralis.
The in situ localization of antigenic substances in Trichinella spiralis muscle larvae was demonstrated at the subcellular level. Larvae recovered from mouse muscle were fixed with half-strength Karnovsky fixative, dehydrated with alcohol, and embedded in LR White resin. Ultrathin sections were incubated with sera from infected Wistar rats and, subsequently, protein A-gold complex. The specificity of the immunostaining was confirmed by a control experiment. Positively immunostaining structures included the stichocyte granules, body cuticle, hindgut cuticle, hypodermis, hemolymph, glycogen aggregates, esophagus-occupying substance (EOS), midgut-occupying substance (MOS), brush border, cytoplasmic granules in the cord, intestinal gland cell granules, and discrete areas in the genital primordial cell. However, the esophageal cuticle, nucleus, nucleolus, mitochondria, rough endoplasmic reticulum, and muscle fibers were negative by immunostaining.
Animals↗
Trichinella spiralis: antigenic substances associated with the alimentary tract.
Postembedding immunogold and immunoperoxidase staining methods revealed that substances occupying the lumen of the esophagus and the midgut were antigenic for both Wistar rats and humans. Specificity of the alimentary tract-associated antigen was assessed by reacting these substances with a panel of serum pools from patients with non-Trichinella helminth infections, including anisakiasis, paragonimiasis, gnathostomiasis, fascioliasis, dirofilariasis, and trichuriasis. The substances had no, or negligible, cross-reactivity among the serum pools with the only exception of severe trichuriasis serum. Cytochemical staining revealed that the substances are PAS positive and are stained red by azan, and the midgut-occupying substance was equipped with exposed concanavalin A-binding sites. The present data suggest the alimentary tract-associated antigen can be purified by lectin affinity chromatography and may be used as a fairly specific antigen for immunodiagnostic purposes.
Animals↗
Studies on prodrugs. 11. Synthesis and antimicrobial activity of N-[(4-methyl-5-methylene-2-oxo-1,3-dioxolan-4-yl)oxy]norfloxacin .
The chemical oxidation of N-[(5-methyl-2-oxo-1,3-dioxol-4-yl)methyl] norfloxacin (2) was carried out to afford N-[(4-methyl-5-methylene-2-oxo-1,3-dioxolan-4-yl)oxy]norfloxacin (4). In vitro, 4 exhibited lower activity than that of norfloxacin (NFLX, 1) for both Gram-positive and Gram-negative bacteria. However, in vivo the activity of 4 was higher than that of NFLX. Bioavailability studies in mice showed that 4 liberated a higher concentration of NFLX in plasma than NFLX itself when administered orally. From these data, 4 obtained by the chemical oxidation of 2 functioned as a prodrug of NFLX as well as did 2. The mechanism of the formation of 4 is interpreted in terms of [2,3]-sigmatropic rearrangement.
Animals↗
Identification of regions functioning in substrate interaction of rabbit liver cytochrome P-450 (laurate (omega-1)-hydroxylase).
The nucleotide sequence of cDNA for rabbit liver cytochrome P-450 (laurate (omega-1) hydroxylase) was replaced with that for rabbit liver cytochrome P-450 (testosterone 16 alpha-hydroxylase) in various regions coding for the amino acid sequence between residues 43 and 261. Six chimeric cDNAs thus constructed were cloned into expression vector pAAH5, and expressed in Saccharomyces cerevisiae AH22 cells under the control of yeast ADH1 promoter. Chimeric P-450s synthesized in the transformed yeast cells were purified partially and their catalytic and spectral properties were examined and compared with those of the chimeric P-450 which is considered to possess the same catalytic properties as the wild-type P-450. In the oxidized state the chimeric P-450s exhibited a low-and high-spin mixed-type absorption spectrum of cytochrome P-450 and the spectrum was converted to a typical high-spin type on addition of laurate or caprate, indicating the binding of the fatty acids to the substrate site of the chimeric P-450s. However the affinities of the fatty acids for the chimeras devoid of the sequence of P-450 (laurate (omega-1)-hydroxylase) in either of the regions spanning residues 90-125 and 210-261 were 10 to 20 times lower than those for the chimeras containing the sequence of the wild-type P-450 in both regions. The latter chimeras have about the same affinities as the chimera which is essentially the wild-type P-450.(ABSTRACT TRUNCATED AT 250 WORDS)
Animals↗
Structure-function relationships of cytochrome P-450 laurate (omega-1)-hydroxylase.
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Animals↗
[A case of congenital laryngeal web with subglottic stenosis].
A severe case of congenital laryngeal web with subglottic stenosis was reported. The patient had weak crying after birth and stridor was noticed from one month old. He had tracheostomy at two month old and the incision of the web was attempted under laryngomicrosurgery with CO2 LASER, although the attempt failed due to combined subglottic stenosis. The "trough method" was applied at the age of one year. The web was incised and subglottic granulation including the excessive cartilaginous tissue was removed through a laryngotracheal fissure. A piece of silicone membrane was inserted between the vocal cords preventing from adhesion and a silicone tube stent was also inserted into the subglottic space and trachea to keep the laryngotracheal space open widely. After one month and a half, the silicon membrane and silicon tube were removed and a silicon T-tube was inserted instead of the silicon tube. The anterior wall of the laryngotracheal space was closed using a hinge flap. The patient can vocalize and does not complain of dyspnea at present, one year after the removal of the T-tube.
Humans↗
[Enzyme immunoassay for arginine vasopressin (AVP). 1. Enzyme labelling of AVP and its application for AVP standard curve].
Enzyme immunoassay for arginine vasopressin (AVP) was developed in this study. Enzyme-labelling of AVP and then application of the enzyme-labelled AVP to make an AVP standard curve were done. Also, the anti-AVP antibody was obtained from the immunized rabbits. The enzyme (beta-D-galactosidase) was combined with AVP by using the N-hydroxysuccinimide ester of N-(4-carboxycyclohexylmethyl)-maleimide. The AVP-beta-D-galactosidase complex was examined for the competitive binding with AVP to the anti-AVP antibody. Then, the solid phase (polystylene bead) coupled with IgG fraction of anti-rabbit IgG serum (goat) was used to separate the antigen-antibody complex. The enzyme activity of this complex was measured to obtain an AVP standard curve. As a result, enzyme immunoassay for AVP described here was sufficiently sensitive and specific. Thus, this enzyme immunoassay could be applied for the determination of physiological levels of AVP in plasma. It is advantageous that the AVP-beta-D-galactosidase complex was stable for several years with respect to enzyme activity and immunological activity. The specific anti-AVP antibody with high titer was gained from the immunized rabbits using bovine thyroid globulin as a carrier protein.
Arginine Vasopressin↗
[A case report of large cell lung cancer detected in the course of antituberculous therapy after a giant bullectomy].
A case of a 60-year-old who male developed lung cancer after surgical resection of a emphysematous giant bulla. At first, he showed a emphysematous giant bulla associated with infected bulla in the right lung on the chest roentgenograph. After a giant bullectomy the resected specimen, suggested possible pulmonary tuberculosis. Thereafter, he was followed for one year with antituberculous therapy. Nevertheless, the chest roentgenograph taken one year after surgery, showed a new tumor density (1.5 x 2.0 cm) in the right lung. Due to the rapid growth of the lung tumor, right upper lobectomy was performed. The histological diagnosis was a large cell lung cancer (giant cell type). The postoperative staging of the tumor proved to be T2N0M0. He was treated with systemic chemotherapy of CDDP 120 mg, VDS 4.5 mg, and MMC 12 mg. Unfortunately, he died of cancer recurrence 5 months after lobectomy. Clinical evaluation of the emphysematous giant bulla associated with lung cancer was performed.
Carcinoma, Small Cell↗
[Experimental study on the changes in circulatory kinetics due to an increase of intraabdominal pressure: the effective monitoring of the pressure of inferior vena cava].
We examined the change in circulatory kinetics due to an increase in intraabdominal pressure by adding pressure quantitatively to the abdominal wall by means of the manchette technique by using mongrel dogs, and obtained the following results: 1) By adding to the pressure to the abdominal wall, the pressure of inferior vena cava at the confluence of right and left common iliac veins showed a significantly higher value (p less than 0.05) than those at other 4 areas of the vena cava from just above the diaphragm to the above confluence. 2) All the dogs in the group with 20mmHg pressure survived for 24 hours, while those in the group with 40mmHg pressure died in all after survival between 13.8 +/- 2.05 hours. 3) The pressures of the inferior vena cava at the confluence of right and left common iliac veins with 20 and 40mmHg showed no significant difference immediately after the pressurization, but thereafter showed a increasing tendency in the group with 40mmHg (p less than 0.05). This increase in the pressure of the inferior vena cava lead to peripheral circulatory insufficiency. 4) The measurement of the pressure of the inferior vena cava at the confluence of right and left iliac veins in case of increased intraabdominal pressure seems to be an effective index for the risk of intraabdominal pressure.
Abdomen↗
[Enzyme immunoassay for arginine vasopressin (AVP). 2. Serum interference and the measurement of plasma samples].
The effect of serum interference on enzyme immunoassay (EIA) for arginine vasopressin (AVP) was studied. Before measurement, plasma was applied on an affinity chromatography to remove AVP and then treated with the acetone-ether method which is usually used for AVP extraction of plasma samples. The AVP-free plasma extract was used to obtain a plasma AVP standard which was compared with various buffer standards of AVP. As a result, the enzyme activities of plasma AVP standard were always lower than those of buffer standards even though the concentration of buffer was increased or added by gelatin. Therefore, EIA for AVP was clinically applicable by using the AVP-free plasma for the standard. Plasma samples were drawn in various clinical conditions and measured for AVP by plasma AVP standard to examine reproducibility and correlation with radioimmunoassay (RIA). The values measured repetitively for the same samples were reproducible. The values measured by EIA correlated well with those measured by RIA (y = 0.67 + 1.13x, r = 0.96). These indicates that EIA for AVP could be applied clinically to measure physiological levels of plasma AVP.
Arginine Vasopressin↗
[A case of adenocarcinoma of the nasal cavity associated with syndrome of inappropriate secretion of antidiuretic hormone(SIADH)].
An inappropriate antidiuretic hormone secretion (SIADH) has been recognized as the cause of hypotonic hyponatremia, and the occurrence of this syndrome, accompanied by an ADH-producing adenocarcinoma in the nasal cavity, is reported. In February, 1987, a 50-year-old male, showing sights of delirium, disorientation, and irritability was admitted to the hospital. The patient was observed to be healthy, except for a neck lymphnode metastasis that was present up to the time of his hospitalization. The hyponatremia was incidentally found, although dehydration or intravascular volume depletion were not noted. These neuropsychiatric symptoms were considered to be associated with hyponatremia due to SIADH. He had had a partial maxillectomy, a neck dissection, and irradiation to the nose and nasal cavity 32 months earlier, and then underwent a surgical resection of the neck metastasis; he had a total of 10 other operations before the onset of the symptoms. Upon initial inspection, since neither an intracranial invasion nor a brain metastasis was found, we diagnosed that his symptoms were due to an autonomic disturbance caused by surgical and mental "stress". When he died of cardiac failure due to a mediastinal invasion 8 months after the onset of SIADH, tumor tissues was extirpated in an autopsy and was then cultured. In this manner, it was proved that the tumor cells had been producing ADH. This procedure clarified that the syndrome had resulted from an ADH-producing tumor of the nasal cavity.
Adenocarcinoma↗
The morphology of Trichinella spiralis: ultrastructural study of the mid- and hindgut of the muscle larvae.
The ultrastructure of the gut of Trichinella spiralis muscle larvae is comprehensively described, based on extensive observations. The mid-gut was composed of a single cell layer of epithelium over the basal lamina, the brush border, the septate junction, prominent glycogen aggregates, and other common cell organelles. The epithelial cells of the midgut were basically uniform, but the lumen presented a remarkably diversified appearance. In the ampullar portion, the midgut accommodated an amorphous substance of medium electron density, the middle portion was collapsed, and the terminal portion again dilated, but without any particular contents. The mid- and hindgut were devoid of muscle cells that could be responsible for the peristaltic movement of the gut.
Animals↗