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Biomedical subjects

T Takeda

Publications and source records attributed to T Takeda.

At least 1,351 records · Page 75Linked to original sources
PubMed1978

Comparison of bacterial cardiotoxins: thermostable direct hemolysin from Vibrio parahaemolyticus, streptolysin O and hemolysin from Listeria monocytogenes.

Some properties of the bacterial cardiotoxins, thermostable direct hemolysin from Vibrio parahaemolyticus (vibriolysin), and streptolysin O and hemolysin from Listeria monocytogenes (listeriolysin), were compared. These toxins had rapid lethal effects on mice when injected intravenously. The electrocardiographic changes of rats after intravenous injections of these toxins were very similar, showing bradycardia and inhibition of atrio-ventricular conduction. These toxins also caused cessation of the spontaneous beating and degeneration of cultured foetal mouse heart cells. When equal hemolytic units of these three toxins were administered, vibriolysin had the most potent effects on mice and cultured mouse heart cells. Differences in the kinetics of the hemolysis by each toxin and in the effects of cholesterol of their hemolytic actions suggest that the mode of action of vibriolysin is different from those of streptolysin O and listeriolysin.

Bacterial Toxins↗
PubMed1977

Convergence of retinal inputs onto visual cortical cells: II. A study of the cells disynaptically excited from the lateral geniculate body.

Spike discharges were recorded extracellularly from cells located in the superficial layer of cat's visual cortex. These cells were disynaptically excited and trisynaptically inhibited from the lateral geniculate cells. The vast majority of them responded to stationary and moving light stimuli. Their ON and OFF responses to a flash of light slit consisted of three components: (1) initial excitation, (2) depression and (3) later rebound. The three components were evoked from a broad area of the retinal receptive field. In one group of these cells, exploration of the receptive field with light slits of different lengths revealed a strong depressant zone at one or both ends of the excitatory receptive area, the characteristics property of 'hypercomplex' cell of Hubel and Wiesel. Another population of cells, however, did not show such length-specificity, and apparently correspond to 'complex' cell. In both groups of cells electrical stimulation at the excitatory receptive area produced a sequence of excitation, depression and later rebound, but only depression was evoked from the depressant zones. The latency of the excitation (6 msec) and the depression (7 msec) are in accordance with the view that the excitation is transmitted through disynaptic pathway and the depression through trisynaptic pathway after being mediated by the lateral geniculate cells.

Animals↗
PubMed1977

Administration of prostaglandin F2alpha for the recovery of fertilized eggs from the vaginas of rabbits.

Nonsurgical recovery of fertilized eggs from the vaginas of prostaglandin F2alpha (PGF2alpha)-treated rabbits was attempted. Eighty-two eggs were collected by the vaginal washing method, and the earliest egg was recovered 20 hours after mating. Twenty eggs recovered vaginally 24 to 107 hours after mating were transferred to six synchronized, pseudopregnant rabbits to test the viability of the eggs; four newborns, two fetuses, and one implantation site were obtained. These results indicate that some viable eggs are discharged into the vaginas of rabbits treated with PGF2alpha.

Animals↗
PubMed1977

Antibody titer and clinical course of pemphigus.

The relationship between the titer of pemphigus antibody and the clinical course of the disease was investigated in 15 patients with various types of pemphigus. Although antibody titers generally ran parallel to fluctuations of the clinical course, the elevation of antibody titer appeared to follow the re-appearance or exacerbation of the lesions on 10 occasions in 6 patients, while it appeared to precede the latter only on 2 occasions in 2 patients. In addition, the disappearance of the antibody was considerably delayed after disappearance of skin lesions in 2 cases. These findings suggest that the pemphigus antibody is the result of skin damage, rather than the cause of pemphigus itself.

Adult↗
PubMed1976

Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin.

Lethal toxin was purified extensively from the culture filtrate of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus. The purified toxin was a protein, and its homogeneity was demonstrated by sodium dodecyl sulfate-polyacrylamide gel disc electrophoresis and analytical ultracentrifugation. It was demonstrated that the thermostable direct hemolysin was identical to the lethal toxin and that it was the main, if not only, lethal toxin in the culture filtrate. The purified toxin had a lethal effect when injected into mice either intravenously or intraperitoneally. Its lethal effect was very rapid, a dose of 5 mug of toxin per mouse killing the animals within 1 min. The lethal activity was inhibited by a ganglioside mixture. Some physicochemical properties of the purified toxin are reported.

Animals↗
PubMed1976

Inactivation of the biological activities of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside Gt1.

Biological activities of the thermostable direct hemolysin produced by Vibrio parahaemolyticus, such as its hemolytic activity and lethal activity, were inhibited by neuraminidase-sensitive gangliosides, of which GT1 ganglioside was the most inhibitory. Neuraminidase-resistant gangliosides did not affect the activities of the hemolysin. Results showed that horse erythrocytes, which are resistant to the hemolysin, do not contain the neuraminidase-sensitive gangliosides GT1 and GD1a. Therefore, we propose that neuraminidase-sensitive gangliosides, and especially GT1 ganglioside, may be the receptor sites on the membranes for the thermostable direct hemolysin of V. parahaemolyticus.

Binding Sites, Antibody↗