Search PubMed⌕ Search

Biomedical subjects

T Takeda

Publications and source records attributed to T Takeda.

At least 1,315 records · Page 73Linked to original sources
PubMed1980

Bilateral visual inputs to the dorsal cap of inferior olive: differential localization and inhibitory interactions.

There are two pathways known to mediate optic signals to the rabbit flocculus through the dorsal cap of the inferior olive; one (I) projects from the contralateral and the other (II) from the ipsilateral eye, both to the contralateral flocculus. The present study on anesthetized rabbits revealed that the two pathways are relayed at different loci of the dorsal cap, I at its caudal and II its rostral half. The ventrolateral outgrowth of the inferior olive also projects to the flocculus, but a prominent field potential could not be produced by optic signals. The present study further demonstrated that transmission in pathway I accompanies an inhibitory action upon that in II. Since direct stimulation of these pathways at preolivary levels revealed similar inhibition, and since this was reflected in the depression of antidromic invasion of dorsal cap neurons, it is concluded that inhibition occurs postsynaptically in dorsal cap neurons. Inhibition is unidirectional, as activities in pathway II did not influence those in I.

Afferent Pathways↗
PubMed1980

Species differences in inner ear fluids.

Inner ear fluids of guinea pigs and cats were analyzed for sodium, potassium, chloride, glucose, and total protein to determine species differences in chemical compositions. In the scala vestibuli perilymph and scala tympani perilymph, sodium potassium, and choride levels in the guinea pig were lower than in the cat. The protein levels in the scala vestibuli perilymph and scala tympani perilymph of the guinea pig were lower than those of the cat. The glucose levels in the guinea pig were higher in the scala vestibuli perilymph and scala tympani perilymph, as compared to findings in the cat. Regarding the utricular endolymph, there were significant differences between guinea pigs and cats in sodium and potassium concentrations; the concentration in the former being higher in sodium and lower in potassium. These findings are pertinent for the phylogenetic studies on inner ear fluid biochemistry.

Animals↗
PubMed1980

Cytological diagnosis of leiomyogenic tumors of the stomach.

The cytological findings in 7 cases of leiomyoma and 4 cases of leiomyosarcoma can be summarized as follows: For differential diagnosis of these lesions, in comparison with leiomyoma cells, leiomyosarcoma cells were found to have 1) increased minor axis diameters of the nuclei and nuclear anisokaryosis, 2) dark nuclear staining, 3) enlarged and darkly stained chromocenters, 4) dark staining and thickening of the nuclear rim, 5) enlargement of nuclear clear areas, 6) increased numbers of oval nuclei and greater pleomorphism, 7) an increase in size and number of the nucleoli, 8) a strong tendency for cell atypism, such as anisocytosis and pleomorphism. In the light of these findings, it is believed that differential diagnosis is indeed possible.

Cell Nucleus↗
PubMed1980

[A study of the effect of dihydrotestosterone on cultured human fibroblasts--with special reference to the hormone effect on the synthesis of proteins, collagen and DNA in fibroblasts (author's transl)].

Fibroblasts grown from abdominal and vulvar skin from normal women were tested for their dihydrotestosterone binding activity. Fibroblasts from either source had 8S cytoplasmic dihydrotestosterone receptors as confirmed by sucrose gradient analysis and the concentration of dihydrotestosterone binding sites and the apparent dissociation constant were 21.6 +/- 1.1 fmoles/mg protein and 0.50 +/- 0.028 nM, respectively, for fibroblasts from abdominal skin and 35.9 +/- 1.7 fmoles/mg protein and 0.24 +/- 0.015 nM, respectively, for fibroblasts from vulvar skin. The dihydrotestosterone binding activity was greater in fibroblasts from vulvar skin than from abdominal skin (P less than 0.01). Fibroblasts formed dense monolayers were treated with various concentrations of dihydrotestosterone (10, 100, 1,000 ng/ml) for six days and were assessed for hormone effect. The production of proteins was elevated in fibroblasts from either source with a slightly greater hormonal response occurring in fibroblasts from vulvar skin than from abdominal skin. Collagen production was elevated significantly in fibroblasts from vulvar skin but only marginally in fibroblasts from abdominal skin. The synthesis of DNA was not affected in fibroblasts from either source. These results than response to dihydrotestosterone was greater in fibroblasts with a higher hormone binding activity may reflect the hormone effect being mediated through dihydrotestosterone receptors.

Cells, Cultured↗
PubMed1979

Origin of descending afferents to the rostral part of dorsal cap of inferior olive which transfers contralateral optic activities to the flocculus. A horseradish peroxidase study.

Neurons in the ventromedial tegmental area that project to the rostral part of the dorsal cap of the ipsilateral inferior olive were labeled by the technique of retrograde transport of horseradish peroxidase (HRP). Following iontophoresis of HRP into the rostral part of the dorsal cap a cluster of labeled cells are found ipsilaterally in the area ventromedial to the red nucleus as well as in the area between the substantia nigra and the medial lemniscus. Following HRP injections into the medial accessory olive just beneath the dorsal cap, labeled cells are found exclusively contralaterally in the deep layers of the superior colliculus and in the adjacent reticular formation. These findings clearly show that neuronal groups in the ventromedial tegmental area are involved in the pathway which from the contralateral eye via the rostral half of the dorsal cap conveys impulses to the flocculus, recorded as climbing fiber activation.

Animals↗
PubMed1979

Purification and partial characterization of heat-stable enterotoxin of enterotoxigenic Escherichia coli.

Heat-stable enterotoxin was purified from a strain of enterotoxigenic Escherichia coli 53402 A-1 from human intestine. The cells were cultured in Casamino Acids-yeast extract-salts medium, and the purification procedure consisted of protamine sulfate treatment of the culture supernatant, ultrafiltration with an Amicon PM-10 membrane, diethylaminoethyl-cellulose column chromatography, hydroxyapatite column chromatography, Bio-Gel P-10 gel filtration, 90% ethanol extraction, and preparative polyacrylamide gel disc electrophoresis. About 300-fold purification was achieved, with a yield of about 12%. However, the homogeneity of the purified heat-stable enterotoxin was not rigorously demonstrated. The purified heat-stable enterotoxin had an absorption maximum at about 275 nm, and its isoelectric point was about 3.90. The molecular weight of the purified heat-stable enterotoxin was ca. 4,000 by Sephadex G-100 gel filtration. The minimum effective dose of purified heat-stable enterotoxin was about 2.5 ng in the suckling mouse assay. The purified heat-stable enterotoxin gave a positive reaction in not only the suckling mouse assay but also the mouse intestinal loop test and the guinea pig skin permeability test.

Electrophoresis, Polyacrylamide Gel↗
PubMed1979

Mechanical factors and patellofemoral osteoarthrosis.

This paper investigates the possible role of mechanical stress in the development of the osteoarthrotic lesions frequently observed in the patellofemoral compartment of the knee joint. First the location of these destructive lesions was determined by studying the location and pattern of contact in the patellofemoral joint. The study was carried out on 39 cadaveric knees for the range of flexion 0 degrees -120 degrees. It was shown that the lesions were localised to the areas corresponding to the range of flexion 40 degrees -80 degrees. These areas have been shown to be subjected to a low stress for most of the time and to a much higher stress for only part of the time. This mode of stressing this area of the cartilage is a consequence of the style of life of the average Western man in which the most predominant activity is level walking, during which the load and in turn the stress are much lower than they are during other ambulatory activities such as ramp and stair ascent and descent. The same area of the cartilage seems to be subject to a similar mode of stress during sedentary occupations. It is suggested that this mode of stressing the cartilage conditions it chemically, and hence mechanically, to transmit low stresses, so that when the much less frequent but higher stresses are applied it cannot transmit them without sustaining some damage.

Activities of Daily Living↗
PubMed1979

Measurement of eye deviation under the nonfixative condition in patients with peripheral labyrinthine disorders, especially Meniere's disease.

Among 232 Japanese patients with peripheral disorders, 98 had a unilateral Meniere's disease. In these patients and 20 healthy adults, recordings of eye deviation were made utilizing DC-ENG under two conditions, i.e. eyes closed (EC) and eyes open in the absolute darkness (AD). (1) 90 % tolerance interval of AD. was significantly smaller than that of EC in controls. (2) Appearance of nystagmus in 5 groups with labyrinthine lesions was compared with the direction of deviation toward and against the affected side in both conditions. (3) Concerning unilateral Meniere's, the relationship among deviation, nystagmus and caloric response was analyzed statistically and the clinical application discussed.

Electronystagmography↗
PubMed1979

Evidence of the origin of the gelatinous masses in the oviducts of mares.

The gelatinous masses known to occur in the mare oviduct were examined by light and scanning electron microscopy. Many (44%) of the masses which occurred in 16 of the 24 oviducts studied were connected with the mucosa of the ampulla near the ampullary-isthmic junction. The masses consisted of lobules of fine fibres probably derived from the fibrous connective tissue of the lamina propria in the oviductal mucosa.

Animals↗
PubMed1979

The effect of angiotensin-converting enzyme inhibition on regional blood flow in salt-depleted and salt-loaded normotensive conscious rats.

The effect of angiotensin-converting enzyme inhibition on regional blood flow was studied in a total of 21 normotensive Wistar rats fed on either low or high salt diet. A new potent angiotensin-converting enzyme inhibitor (CEI), SQ 14,225 was administered intravenously in a dose of 2 mg/Kg to the conscious animals, and changes in fractional distribution of cardiac output were determined with a microsphere method. Prior to administration of CEI, there was no significant difference in mean arterial pressure (MAP) or regional blood flow between salt-depleted and salt-loaded rats. With CEI, MAP did not change significantly in either group. Fractional distribution of cardiac output increased to the kidneys (p less than 0.002), and decreased to the stomach, spleen, and skeletal muscle (p less than 0.02, p less than 0.002, and p less than 0.01, respectively) in the salt-depleted group, while a pattern of blood flow distribution was not changed in the salt-loaded group. These results suggest that angiotensin II plays an important role in regulating regional blood flow in salt-depleted conscious animals, but not in salt-loaded ones.

Angiotensin-Converting Enzyme Inhibitors↗
PubMed1979

Prevention of tissue factor generation in mononuclear cells by agents known to increase intracellular cyclic AMP.

Agents known to increase intracellular levels of cyclic 3', 5'-adenosine monophosphate (cyclic AMP) were examined for their effect on tissue factor generation by mononuclear cells cultured with E. coli endotoxin. Aminophylline, an inhibitor of phosphodiesterase, and epinephrine, a beta-adrenergic agent, showed an inhibitory effect, and these effects were reversible. Moreover, dibutyryl cyclic AMP also exhibited the effect. Dibutyryl cyclic GMP, however, did not enhance the tissue factor generation by mononuclear cells. On the basis of these observations, it was concluded that the phenomenon of tissue factor generation by mononuclear cells is a biological event, and that intracellular cyclic AMP has a possible role in modulating this phenomenon.

Aminophylline↗