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Biomedical subjects

T Ochiai

Publications and source records attributed to T Ochiai.

At least 325 records · Page 18Linked to original sources
PubMed1976

Functional characterization of a naturally occurring antibody cytotoxic for a subpopulation of splenic T cells.

Sera from old (greater than 6 months) New Zealand Black (NZB) mice were shown to contain an IgM antibody which was cytotoxic for thymus-derived (T) lymphocytes in the presence of rabbit complement. This antibody in the presence of complement markedly decreased the response of normal spleen cells to the T cell mitogen concanvalin A (Con A) but not to another T cell mitogen phytohemagglutinin-P (PHA-P). Responses of these treated cells to the polyclonal B cell mitogens, bacterial lipopolysaccharide (LPS), and polyriboinosinic-polyribocytidylic acid (poly[I]-poly[C]) were unimpaired. This naturally occurring NZB antibody was different from conventional anti-theta serum which abolished the response to both PHA-P and Con A. Moreover, the NZB serum affected spleen cells from both theta-C3H and theta-AKR mice. The ability of such serum to influence, differentially, Con A reactive cells as opposed to PHA-P-reactive cells, suggest that the NZB antiserum recognizes a subpopulation of T lymphocytes.

Animals↗
PubMed1976

The mixed kidney cell-lymphocyte reaction in rats.

Optimal conditions for obtaining the proliverative response in mixed kidney cell-lymphocyte culture (MKLC) have been determined in rats. The addition of 2-mercaptoethanol to the culture medium was essential for successful stimulation of lymphocytes by allogeneic kidney cells. Proliferative responses by standard MLC and MKLC were compared in rats with the same or a different Ag-B locus. In most strain combinations, there was a significant correlation between MLC and MKLC reactions, However, the DA-ACI combination, compatible at the Ag-B locus, consistently showed negative MLC and positive MKLC reactions. This suggested the possibility that this technique allowed the expression of kidney specific antigens.

Animals↗
PubMed1976

Oxygenation properties and intraerythrocytic constituents of human blood when stored in different media of ACD and CPD.

In the blood stored in acid-citrate-dextrose solution (ACD blood), the oxygen affinity and red cell 2, 3-diphosphoglycerate (2, 3-DPG) content showed parallel exponential decays with half-lives of 3 to 4 days. In the blood stored in citrate-phosphate-dextrose solution (CPD blood), the two parameters increased during the first 4 days before showing the same decay as that seen in the ACD blood. There was no significant change in the transmembrane pH gradient of the red cells, and thus the intracellular pH at the plasma pH of 7.40 was always in the range of 7.17 +/- 0.02 throughout the period of storage in ACD medium. In both ACD and CPD blood, the Hill exponent n was always normal (approximately 2.8) while the Bohr coefficient (delta log P50/delta pH) rose along with the lapse of time for preservation. The oxygen affinity of the CPD blood was less influenced by the red cell 2, 3-DPG than was that of the ACD blood. This phenomenon was thought to derive from higher concentration of salts within the CPD-stored red cells. The efficiency of blood oxygen transport in ACD and CPD blood was compared.

Blood Preservation↗
PubMed1976

[Pharmacological studies of antipsychotic drug, penfluridol. 2. General pharmacological properties].

General pharmacological properties of penfluridol (TLP-607) a long-acting antipsychotic drug, were examined in experimental animals and the following results were obtained. 1) TLP-607 produced a lowering of arterial blood pressure and bradycardia but had almost no effect on respiration and peripheral blood flow. 2) TLP-607 slightly hypertension induced by dopamine, adrenaline and noradrenaline, but had no effect on hypotension induced by acetylcholine and histamine. 3) Antagonistic actions of TLP-607 on such spasmogens as acetylcholine, histamine and barium chloride were slightly stronger than those of haloperidol and chlorpromazine. 4) TLP-607 had neither ganglionic nor neuromuscular blocking action. 5) TLP-607 had a slight or no effect in the following experiments; protection against stomach ulcer, activity of ileum and uterus in vivo and in vitro, urinary volume and electrolytes excretion, and gastro-intestinal propulsion. These results suggest that TLP-607 has no striking peripheral actions in experimental animals.

Animals↗
PubMed1975

Specificity and immunosuppressive potency of a rabbit antimouse T cell-specific antiserum.

An attempt was made to prepare a specific heterologous rabbit antimouse T cell antiserum (anti-MTLA) by absorbing rabbit antimouse thymocyte globulin (ATG) with spleen cells from BALB/c TXBM mice. Cytotoxicity data showed that whereas ATG was cytotoxic to both T and B cells, anti-MTLA was highly cytotoxic to only T cells. Whereas spleen cells treated with ATG and complement (C) failed to respond in all assays studied, spleen cells treated with anti-MTLA and C: (1) responded to the B cell mitogens but failed to respond to the T cell mitogens; (2) were able to stimulate allogeneic spleen cells but failed to respond to mixed lymphocyte culture (3) failed to act as T killer cells in the CML reaction but retained their ability to kill antibody-coated target cells; and (4) did not cause a graft-versus-host reaction when injected in allogeneic mice and increased their survival significantly. Furthermore, anti-MTLA was just as immunosuppressive in vivo as ATG in its ability to suppress the immune response to sheep red blood cells and prolong skin allograft survival. Anti-MTLA was found to be different in specificities from anti-theta serum by several points: (1) it was cytotoxic for T cells from both theta-C3H and theta-AKR mice; (2) it was highly immunosuppressive in vivo when compared to anti-theta serum; (3) absorption of anti-MTLA with mouse brain did not decrease the immunosuppressive activity; and (4) rabbit antimouse brain antiserum failed to show any immunosuppressive activity. These data indicate that anti-MTLA is a specific antiserum against a unique marker on T cells distinct from the theta marker.

Animals↗