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Biomedical subjects

T Noda

Publications and source records attributed to T Noda.

At least 343 records · Page 19Linked to original sources
PubMed1989

Structure-activity relationship of calcitonin gene related peptide.

Chicken, human(alpha) CGRPs and their analogues were synthesized to investigate the relationship between structure and serum calcium and phosphate lowering effects. The native hormones contain 37 amino acid residues with only four positions (3, 14, 15 and 23) that differ. Chicken CGRP exhibits stronger and longer lasting activities than the human hormone. A chicken CGRP analogue with replacement of Asp at position 14 by Gly (as in human CGRP) showed great reduction of activity. The converse replacement of Gly at position 14 by Asp in human CGRP enhanced this analogue activity. Among the synthetic analogues, des-1-Ala-des-alpha-amino chicken CGRP, exhibited the most potent and long lasting biological activity.

Amino Acid Sequence↗
PubMed1989

Skin typing, sun exposure, and sunscreen use in a population of Japanese.

Distribution of Japanese skin types (JSTs), sun-exposure habits, and sunscreen use were surveyed in 379 new outpatients. The largest number of subjects belonged to JST class J-II (65%), J-I was second (24%), and J-III was third (11%). Eighty-eight percent of those studied had occasional or habitual sun exposure during the summer, and many were exposed for more than three hours per exposure day (80% of those with occasional exposure; 62% of those with habitual exposure). More younger persons had occasional sun exposure than older persons. More males had habitual sun exposure than females. Sunscreens were used by 41% of the subjects, but 77% of these used them without accurate understanding of the definition of the sun protection factor. More J-I subjects used sunscreens than J-II and J-III subjects. More females than males used them.

Adolescent↗
PubMed1989

Characterization of the CA125 antigen secreted from a newly established human ovarian cancer cell line (SHIN-3).

A cell line (designated SHIN-3) was established from a 56-year-old Japanese woman diagnosed as having serous cystadenocarcinoma of the ovary. SHIN-3 produced the tumor marker, CA125. When samples were subjected to one-dimensional gel electrophoresis followed by immunoblotting with anti-CA125 antibody, antigen binding appeared in the low-molecular-mass fraction around 50 KDa as well as in the high-molecular-mass fraction (more than 200 KDa). Subsequent resolution of this lower-molecular-mass component by two-dimensional gel electrophoresis gave rise to bands of 49 KDa near an isoelectric point of 7.3. Treatment of the CA125 antigen with 10 mM periodic acid resulted in no loss of activity. However, reduction and alkylation in 6 M guanidine-HCl or treatment at 100 degrees C for 20 min resulted in complete loss of activity. Treatment with various glycosidases did not inhibit activity, whereas digestion with proteases (except for papain) produced complete loss of activity, suggesting that the CA125 antigenic determinant is composed of conformationally dependent peptides.

Antigens, Tumor-Associated, Carbohydrate↗
PubMed1989

Identification of a transforming gene of human papillomavirus type 16.

Previously, we observed sequential two-step alteration, growth stimulation, and progression to a more malignant state in NIH 3T3 cells transfected by human papillomavirus type 16 (HPV-16) DNA. In this study, we prepared a cDNA library from RNA extracted from cells transfected with the HPV-16 DNA and isolated cDNA clones which had growth-stimulating activity. Analysis of these cDNA clones indicated that the E7 open reading frame alone is responsible for inducing both steps of this cell transformation.

Animals↗
PubMed1989

Calcitonin gene-related peptide receptor antagonist human CGRP-(8-37).

From this study, we predicted that the human calcitonin gene-related peptide (hCGRP) fragment hCGRP-(8-37) would be a selective antagonist for CGRP receptors but an agonist for calcitonin (CT) receptors. In rat liver plasma membrane, where CGRP receptors predominate and CT appears to act through these receptors, hCGRP-(8-37) dose dependently displaced 125I-[Tyr0]rat CGRP binding. However, hCGRP-(8-37) had no effect on adenylate cyclase activity in liver plasma membrane. Furthermore, hCGRP-(8-37) inhibited adenylate cyclase activation induced not only by hCGRP but also by hCT. On the other hand, in LLC-PK1 cells, where calcitonin receptors are abundant and CGRP appears to act via these receptors, the bindings of 125I-[Tyr0]rat CGRP and 125I-hCT were both inhibited by hCGRP-(8-37). In contrast to liver membranes, interaction of hCGRP-(8-37) with these receptors led to stimulation of adenosine 3',5'-cyclic monophosphate (cAMP) production in LLC-PK1 cells, and moreover, this fragment did not inhibit the increased production of cAMP induced not only by hCT but also by hCGRP. Thus hCGRP-(8-37) appears to be a useful tool for determining whether the action of CGRP as well as that of CT is mediated via specific CGRP receptors or CT receptors.

Adenylyl Cyclases↗
PubMed1989

Mechanical adaptation of heart rate change for coronary circulation in patients with and without ventricular hypertrophy.

To clarify the mechanical adaptation and interference of coronary vessels, we studied hemodynamics of coronary circulation in control and 4 different pacing rates (80, 100, 120, 150/min) in 5 patients with angina pectoris (AP) and in 5 patients with hypertrophic cardiomyopathy (HCM). Coronary sinus flow (CSF) was measured by a Webster's thermodilution catheter, and we applied ascorbic acid-platinum reaction for the mean transit time measurement in left coronary flow (t0-t2). Coronary vascular bed (CVB) was obtained by multiplying CSF and t0-t2. CSF in AP gradually increased from 104 +/- 21 ml/min at 72/min to 148 +/- 42 ml/min at 120/min, while CSF in HCM changed slightly from 91 +/- 25 ml/min at 64/min to 94 ml/min at 120/min. Average t0-t2 in HCM was 6.0 +/- 1.6 sec in control which was significantly lower than that in AP (7.8 +/- 0.7 sec). Calculated CVB in AP increased at any given heart rate up to 120/min (13.5 +/- 2.4, 15.8 +/- 1.7, 15.0 +/- 4.7, 15.1 +/- 4.3 ml), but CVB in HCM decreased from 9.1 +/- 2.3 ml at 64/min to 8.1 +/- 1.7 ml at 120/min. These data suggest that myocardial compression and suction at different heart rates and with different cardiac muscle structures play an important role for beat to beat adjustment of coronary circulation in cardiac cycle.

Adaptation, Physiological↗
PubMed1989

[Evaluation of super-elasticity characteristics of orthodontic Ni-Ti alloy wire].

Mechanical properties of orthodontic Ni-Ti alloy wires, especially their super-elasticity characteristics, were investigated in order to use them effectively in the clinics. In this study, twenty commercial orthodontic Ni-Ti wires were tested and two parameters were devised for the load-deflection curve and introduced to evaluate the practical mechanical properties, one being the "super-elastic index: SEI" for super-elasticity, and the other being the "E-load" for the load in the super-elastic region. There was a "flat area" in the load-deflection curve of the super-elastic type of Ni-Ti alloy wires, whose SEI was above seventy and a continuous force could be used through the wide range of deflection. The load of the work-hardened type of Ni-Ti wires, SEI below thirty, was nearly proportional to the deflection. And the middle type showed intermediate properties of the two. The E-load values of the super-elastic type wires are thought to indicate the load level in the super-elastic region. The quantitative evaluation of the super-elasticity characteristics of the orthodontic Ni-Ti wires became possible by the introduction of these parameters.

Dental Alloys↗
PubMed1989

[Clinical studies on 16 cases of odontoma in children].

16 cases of odontoma (male: 12, female: 4) in young patients from 1 year 2 months to 14 years of age at the Pedodontic Clinic of Niigata University Dental Hospital in a recent 9 year period were investigated clinically. As for the pathologic histology, 12 cases were of the compound type, 2 were of the complex type, 1 was of the intermediate type between compound and complex, and 1 was of ameloblastic fibro-odontoma type. 9 cases occurred in the maxilla, 7 cases occurred in the mandible. Most of the compound type were observed in the area of the maxillary incisors and cuspids, and the complex type cases were noted in the anterior part of the maxilla. In many cases, there were clinical symptoms such as retarded eruption. Some cases of odontoma in children were small in scale and showed a low degree of calcification. Therefore they showed a tendency not to be found out at radiography, and it is difficult to diagnose them as odontoma. By finding odontoma prematurely and removing it in time, the retarded eruption teeth were able to erupt spontaneously or to be guided to a normal position.

Child↗
PubMed1989

[Dental findings in a case of idiopathic hypoparathyroidism].

Idiopathic hypoparathyroidism is characterized by a deficiency of the parathyroid hormone, origin unknown, and causes a low serum calcium and a high serum inorganic phosphorus. This report concerns a clinical observation of a case of idiopathic hypoparathyroidism. This case was examined from the dental point of view. The findings were as follows: 1. The roentgenographic findings revealed that there was no demonstrable abnormality concerning bone age indicated between the ages of 5 and 6 years. 2. Severe dental caries were noted in the deciduous teeth and the permanent teeth. 3. Hypoplasia and hypocalcemia were recognized in most of the permanent teeth. 4. Large pulp chambers were observed in the deciduous teeth and the permanent teeth, and thickening of the lamina dura was observed in the permanent teeth. 5. The histological findings of the extracted deciduous teeth and the upper right first molar were such that: (1) there was hypoplasia and hypocalcemia in the dentin of deciduous teeth, and there were multiple resorptions in the cementum. (2) there was hypoplasia in the enamel of the upper right first molar. 6. Electron probe microanalysis of the upper left second molar revealed that the degree of mineralization and concentration of Ca, P and Mg in the dentin of the root showed a lower intensity than in the dentin of the normal one. 7. It was assumed from the dental point of view that this hypoparathyroid condition first appeared at about 11'months approximately 18'months of age.

Child, Preschool↗
PubMed1989

[Examination and treatment of dental caries in the Niigata Prefectural Institute of Total Training for the Mentally Retarded, colony Niigata Shiroiwa-no-Sato].

The purpose of this study was to learn about the prevalence of dental caries in mentally retarded individuals and to follow up the treatment of the caries. The study group comprised 144 severely retarded individuals (79 males and 65 females) aged 6-19 at the time of their admission to the Institute. Following results were obtained by analyses of their dental examination over a period of records in 15 years from 1973 to 1987 and of their treatment charts over a period of 8 years from 1980 to 1987. 1. The DMFT index at the time of their admission showed a tendency to increase gradually with age. In the DMFT index by age, there was no significant difference between that of the Institute and that of the Survey of Dental Disease by Ministry of Health and Welfare Japan in 1981. 2. The FT index was lower and the MT index was higher than the indices of the Survey, and each of differences showed a statistical significance for almost of all ages. Also in the proportion of both indices to the DMFT index, there were significant differences respectively. 3. During the 8 years between 1980 and 1987, 740 teeth were treated with 388 amalgam fillings (52.4%), 174 resin fillings (23.5%), 22 metal crowns (3.0%), 55 endodontic therapies (7.4%) and 101 extractions (13.7%). 4. After Our department started the treatment program in 1980, there were under 0.5 untreated teeth in each individual for each year. Moreover, missing teeth increased slightly in individuals who were on the register more than 2 years. 5. One of the 237 filled teeth (0.4%) and 2 of 21 endodontically treated teeth (9.5%) were extracted because of unfavorable prognosis during 8 years of the follow-up survey period.

Adolescent↗
PubMed1989

[Estrogen and androgen receptors in hepatocellular carcinoma and in noncancerous liver tissue].

We have assayed both estrogen and androgen receptors in hepatocellular carcinomas, in the surrounding noncancerous liver tissue, and in normal liver tissue. Estrogen receptors (ERs) were detected in 5 out of 22 HCC tissues, 14 out of 22 surrounding noncancerous tissues, and in 5 out of 6 normal liver tissues. Androgen receptors (Ars) were detected in 4 out of 21 HCC tissues, in 7 out of 21 surrounding noncancerous tissues, and in 2 out of 5 normal liver tissues. These results suggest that the pathogenesis of some HCC may be dependent on estrogen or androgen. Hence, it may be beneficial to provide hormonal therapy for HCC, especially in cases that show high concentrations of ER and/or AR.

Adult↗
PubMed1989

[Study of the mechanism of tumor marker CA125 in serum].

Ascites fluid, chest effusion, cyst fluid, and serum mainly obtained from malignant-ovarian-cancer patients were applied to quantitate the concentration of the tumor marker CA125. The same procedure was also performed for ascites, cyst fluid, and serum mainly obtained from benign-ovarian-tumor patients. To know how CA125 proceeds from tumor cells into the circulation, a CA125-producing, ovarian-cancer-cell line (SHIN-3) was transplanted sub-cutaneously into nude mice. Then the relationship between the grown tumor size and CA125 concentration in serum was analyzed and the histo-pathological background was also evaluated. Quantitative analysis of the ascites and cyst fluid showed no remarkable difference between the malignant-tumor patients and benign ones: however, the CA125 concentration in serum of malignant-tumor patients was up to 90 times higher than in benign ones. The proceeding ratio of CA125 in its productive site (cyst fluid, for example) to that in the serum of patients with malignant lesions was 24 times as high as with benign lesions. No relationship between tumor sizes and the CA125 concentration in serum in nude mice was observed. And in the mice which had high CA125 in serum, the capsules around the tumor tended to be destroyed and vessel infiltration by tumor cells was remarkable.

Animals↗
PubMed1989

[Treatment of Riga-Fede's disease by resin-coverage of the incisal edges and seven cases of natal and neonatal teeth].

This report concerned 2 cases (1-month and 8-day-old boy and 5-month and 27-day-old boy) of Riga-Fede's disease. The cases were treated with photopolymerized resin coverage of the incisal edges. Generally, the treatment methods for Riga-Fede's disease are discing and extraction of the traumatized teeth. The photopolymerized resin coverage proved to help in the rapid healing of the ulcerations. This method is useful because it is easy and is able to avoid a false pulp exposure of the hypoplastic natal and neonatal teeth by discing. 7 cases of natal and neonatal teeth were encountered at the Pedodontic Clinic of Niigata University Dental Hospital during 8 years from September 1979 to August 1987. 1) 7 cases were 3 boys and 4 girls, aging from 21 days after birth to 3 months 19 days. 2) Only one baby had a low birth weight. 3) The natal teeth accounted for 6 cases and the neonatal teeth accounted for 1 case. All were lower central primary incisors (5 on the right side, 1 on the left side and 1 bilateral). There were no supernumerary teeth. 4) 5 cases were of hypoplastic teeth. 5) Riga-Fede's disease was recognized in 3 cases. 6) The prognosis was 1 case of shedding, 2 cases extracted and 4 cases remaining. 7) Mesial inclination of the neighboring teeth and narrowness of the extracted space wee observed in the cases where extraction was performed at an early age. 8) Microradiogram and undemineralized specimens of the extracted tooth revealed that the tooth was very thin and mineralization was so poor and there was almost no root formation.

Child, Preschool↗
PubMed1989

[A case of horizontal impaction of mandibular left and right deciduous central incisors].

This paper reports on a rare case of horizontal impaction of the mandibular left and right deciduous central incisors. The patient was a 4 year and 7-month-old-girl at the time of first visit to our clinic. She had no previous history, and there was no family history or anomaly in her general physical condition. All the deciduous teeth except the mandibular left and right deciduous central incisors had already erupted. The right deciduous central incisor was impacted completely and the left one erupted with its incisal edge about 2 mm at the lingual gingiva. In the X-ray examination, the horizontal impaction of the mandibular left and right deciduous incisors were noted. There were no cysts, odontoma or tumors. Both of them were extracted to prevent loss of space for the permanent central incisors. Each root of both teeth was curved 90 degrees in the lingual direction at half of length of the root. There was no anomaly of figure and color in their crowns. In the histopathologic examination, there was no anomaly except for the small root resorption at the labial and lingual sides. 3 years and 3 months after extraction of the deciduous central incisors, approximately two thirds of the crowns of the left and right mandibular permanent central incisors have erupted. Both of them incline to the lingual direction and rotate and there is a space of about 5 mm between them. This case is still under observation.

Child, Preschool↗
PubMed1989

[Chromosomal abnormality of trisomy 4 in a patient with acute nonlymphocytic leukemia (FAB: M2)].

We report a patient with acute nonlymphocytic leukemia (FAB classification: M 2) with trisomy 4, which is the first case in our country. A 42-year-old man was admitted to our hospital because of fever and general fatigue in May, 1988. His WBC count was 8,100/microliters with 90% of leukemic cells and bone marrow smear showed 76.1% leukemic cells. The chromosomal analysis of marrow cells by G-banding revealed 47, XY, +4. In spite of administration of chemotherapy complete remission was not obtained, and he died of septic shock and severe liver damage 4 months after making the diagnosis. Chromosomal abnormality of trisomy 4 has been reported to be associated with predominantly either M 4 or M 2, and to be less than 0.1% of incidence in ANLL, according to the Second MIC Cooperative Study Group. It is suggested that trisomy 4 may be caused by exposure to some environmental factors such as toxic substances, since this chromosomal abnormality has been reported in the last 10 to 20 years.

Adult↗
PubMed1989

An acoustic investigation caused by pontic formation.

Prosthetics for cases with large defects of alveolar bone often tend to be difficult. We researched the acoustic effects of various pontic forms to satisfy a patient's expectations for both phonetics and aesthetics.

Alveolar Process↗