Biomedical subjects

T Noda

Publications and source records attributed to T Noda.

At least 325 records · Page 18Linked to original sources

PubMed1990

Hepatotoxicity of geniposide in rats.

The hepatotoxic effects of geniposide were investigated in rats. Increases in serum alanine aminotransferase and aspartate aminotransferase activities as a result of oral administration of 320 mg geniposide/kg body weight were suppressed when geniposide was administered ip or when the rats were pretreated with chloramphenicol. The non-protein sulphydryl content of the liver 4 hr after oral administration of geniposide decrease in a dose-dependent manner. Genipin, the aglycone of geniposide, had a marked reactivity with sulphydryl groups of glutathione and cysteine in vitro. The hepatotoxic effects of ip administration of genipin at a dose of 80 mg/kg body weight were comparable with those of oral administration of geniposide at a dose of 320 mg/kg. Buthionine sulphoximine pretreatment enhanced the toxicity of geniposide, while cysteine pretreatment completely suppressed it. These results suggest that the conversion of geniposide to genipin is causally related to the hepatotoxicity of geniposide and that hepatic non-protein sulphydryls are important in modulating the toxicity.

Administration, Oral↗

PubMed1990

Reproduction of the skin lesions of polymorphous light eruption: a case report and a review of the Japanese literature.

A case of polymorphous light eruption with plaque-type lesions is reported. A 55-year-old male had pruritic erythemas and plaques on the head, the face, the nape, the back of the hands, and the left loin within days following sun exposure. The patient's minimal erythema dose (MED) (60 mJ/cm2) of sunlamp irradiation for ultraviolet (UV) B was normal. Three once-daily exposures to 2 MED of UVB (Epstein's repeat phototest technique) produced pruritic papules with a similar microscopic appearance to that of the patient's skin lesion, indicating that the action spectrum causing the lesion seemed to be within the UVB waveband.

Humans↗

PubMed1990

Establishment and characterization of a human ovarian serous cystadenocarcinoma cell line that produces the tumor markers CA-125 and tissue polypeptide antigen.

A cultured cell line (SHIN-3) derived from a human ovarian serous cystadenocarcinoma which consistently produces two tumor markers, CA-125 and tissue polypeptide antigen (TPA) was established. After 1 week of culture of 1 x 10(5) cells, high levels of tumor marker were observed (the total CA-125 release was 1,500 U and the total TPA release was 37.5 U). Expression of CA-125 and TPA was also demonstrated in cultured cells immunohistochemically. The volume of CA-125 release per cell was highest just before the start of the logarithmic growth phase. TPA production was increased in the logarithmic growth phase, but its relationship to the total number of cells was not clear.

Animals↗

PubMed1990

Physical training in patients with valvular heart disease after surgery.

The necessity, the methodology and clinical benefit of physical training were evaluated in 85 patients with valvular heart surgery. In 21 patients who had undergone the exercise stress test before surgery, corrective valvular surgery increased maximal oxygen uptake (max.VO2) from 15.4 before to 18.9 ml/min/kg, while 11 (52%) of them failed to increase exercise capacity over 5 METs. Of the total 85 patients, 41 (58%) again showed reduced exercise capacity in the initial postoperative stress test, suggesting that a return to work might be difficult without rehabilitation in these patients. We then compared two training programs: program A of short duration and frequent exercise and program B of longer duration and less frequent exercise, both using a bicycle exercise set at an intensity of 70% of the max.VO2. Both programs similarly increased max.VO2, while patients preferred program A, suggesting that exercise of longer duration could not be tolerated because of deconditioning. Program A was then prescribed to 62 patients, and it increased max.VO2 from 18.2 to 20.7 ml/min/kg after 4 weeks training without any complication. In 9 patients who served as controls undergoing no physical training, no spontaneous improvement in exercise capacity was observed. Of the 76 patients who received either program A or B, 28 patients failed to increase the max.VO2 by 10% or more. These patients presented atrial fibrillation, a cardiothoracic ratio greater than or equal to 60% or exercise-induced ST depression more frequently, suggesting that residual cardiac dysfunction might inhibit the training effects.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1990

[Experimental positional nystagmus induced by argon laser irradiation to the oval window].

Experimental positional nystagmus (EPN) similar to benign paroxysmal positional nystagmus (BPPN) was induced in 8 of 20 rabbits by argon laser irradiation to the footplate. The power of the argon beam used was 2.0 watts and irradiation time was 1 or 2 sec. EPN and BPPN showed the following similarities maintained (1) When the head was maintained in the affected ear down position and the head positioned from the sitting to the supine position nystagmus directed to the affected ear or vertical nystagmus corresponding to rotatory nystagmus in man were provoked. (2) Delayed onset for provocation of nystagmus. (3) Limited duration. (4) Fatigability. (5) The direction of nystagmus was reversed when the head returned to the initial position (in two rabbits). Histological examination of the temporal bones showed separation of otoconia from the otolithic membrane of the saccule and bleeding in the saccule in both conditions. No lesions in the utricle and semicircular canals were evident. It was assumed that ablation of otoconia of the saccule causes this experimental positional nystagmus.

Animals↗

PubMed1990

[A new monoclonal antibody (SH-9) recognizes the low molecular mass of ovarian cancer antigen CA125].

The SHIN-3 cell line producing CA125 was established from an ovarian cancer patient. Using the SHIN-3 cell line, we found that the low-molecular-mass antigen (about 50 KDa) might be the main antigenic determinant in CA125-immunoreactive species. A new monoclonal antibody to this low-molecular-mass was raised to examine a new cancer associated antigen by a hybridoma technique. Using enzyme linked immuno sorbent assay, ten clones were selected from among 398 clones. Two clones were IgG1 and eight were IgM. By immunostaining (ABC assay), a new antibody (named SH-9) reacted with normal pulmonary bronchus and uterine cervical glands. No positivity, however, was observed in endometriosis (adenomyosis). In tumorous lesions of ovary, SH-9 antibody reacted specifically with mucinous cystadenoma-benign, borderline or malignant. However, no positivity was found in serous cystadenocarcinoma. In any other carcinomas, only lung cancer (adenocarcinoma, squamous cell carcinoma) showed a clear positivity. Immuno blotting analysis showed that SH-9 antibody recognized a low molecular mass. Therefore, SH-9 is seen to be an extremely unique antibody when compared with OC125 biochemically and histochemically.

Antibodies, Monoclonal↗

PubMed1990

[Pure white cell aplasia (PWCA) with an inhibitor against colony-forming unit of granulocyte-macrophage (CFU-GM)].

We reported here a case of pure white cell aplasia (PWCA). A 23-year-old man was admitted to our hospital in September 1989 because of agranulocytosis, fever, and anal pain. He had no history of toxic-drug exposure or blood transfusion. Laboratory studies were all within the normal range except white blood cell count of 2,300/microliters with no neutrophils and low serum IgA level (28 mg/dl). Bone marrow examination showed hypocellular with erythroid predominance and no granulocyte maturation beyond the myelocyte. Complement-dependent suppression of autologous and heterologous granulocyte-macrophage colony-forming units by the patient's serum could be demonstrated. Though corticosteroid administration was ineffective, neutropenia improved by plasmapheresis. Furthermore, recombinant granulocyte colony stimulating factor (rG-CSF) could release him from persistent bacterial infection of anal fistula by transient improvement of neutropenia. These findings suggest a humoral autoimmune mechanism for the pathogenesis of PWCA and the effectiveness of rG-CSF for the patient with severe infections.

Adult↗

PubMed1990

[A clinical observation of epithelial pearls in newborn babies. 1. Appearances and continuous changes].

The purpose of this study was to learn about the appearances and continuous changes of epithelial pearls. The study group comprised 517 normal newborn babies (261 males and 256 females) aged from 24 hours of birth to 22 days. Furthermore, we made the follow-up survey on the continuous changes in 21 newborn babies at the Niigata University Medical Hospital. The following results were obtained by analyses of the examination of the oral cavity in newborn babies during the period from December 1987 to December 1988. 1) Yellow-white in colour, pinhead shaped, 1-3 mm diameter in size, elevated firm nodules were seen in the alveolar ridge mucosa of the maxilla, mandible and/or palate. The average number of epithelial pearls was 1-3. 2) The number of newborn babies who had epithelial pearls was 226 (109 males and 117 females) out of 517 (43.7%). There was no predominant sex preference. 3) The most common location was the maxillary alveolar mucosa (309, 72.5%) followed by the median palatine raphe (97, 22.8%) and mandibular alveolar mucosa (20, 4.7%). 4) In this study, the relationship between the environmental factors of newborn babies in the prenatal and postnatal periods (mother's age, the course of the pregnancy, trouble at birth, the period of pregnancy, baby weight at birth etc.) and the rate of appearances of the epithelial pearls was examined. These factors showed no significant relationships. 5) Based on the findings of the clinical investigation of epithelial pearls during neonatal period, some cases were found and involved thickening and/or thinning in changes in the quantity and number.

Dental Enamel↗

PubMed1990

An acoustically investigated case of phonetic change in higher vertical dimension.

This report presents the phonetic changes of a patient whose occlusal vertical dimension is extended. His chief complaint is esthetic restoration of his anterior teeth, but his occlusal vertical dimension is abnormally shortened by long-time attrition. Therefore we extended his occlusal vertical dimension, and reconstructed his occlusion, and carried out acoustic observations.

Aged↗

PubMed1990

[Clinico-statistical observation of the dental surgery in the pedodontics clinic of Niigata University Dental Hospital during the period from 1979 to 1989].

Clinico-statistical observation was performed on the dental surgery in the Pedodontic Clinic of Niigata University Dental Hospital during the period from August 1979 to September 1989. 1. The number of surgical operations amounted to 656 cases (males 384 and female 272) and the highest age incidence was in the 6-8 year old range. 2. The incidence of the impacted teeth (62.8%) and the abnormality of the frenum (22.8%) occupied over 85% of all the cases. 3. Based on sex, the impacted supernumerary teeth and the abnormality of the buccal frenum, odotoma were more frequent in males, while the abnormality of the upper frenum and the mucous cysts were more frequent in females. 4. About 40% of the operative patients accepted were introduced from other medical institutions. 5. Regarding the methods of surgery, the impacted permanent teeth were frequent revealed by surgical exposure, while the diseases of salivary glands were revealed by extirpation. The benign tumors were removed by extirpation, the diseases of soft tissues by excision, cysts in the jaws were all surgically removed by marsupialization. 6. In regard to the age at the time of the operation, the abnormal frenum were often seen under 4 years of age but large increases were found in the impacted teeth from 4 to 8 years of age. The diseases of salivary glands and of soft tissues were seen more frequently under 10 years of age, and cysts in the jaws were noted after 7 years of age. 7. Children undergoing surgery who suffered from general diseases amounted to 31 cases and were found most commonly in cases of nervous mental diseases.(ABSTRACT TRUNCATED AT 250 WORDS)

Age Factors↗

PubMed1990

[An investigation into the actual condition of children with blood diseases in the pedodontic clinic of Niigata University Dental Hospital].

Children with blood diseases do not infrequently visit the dental clinics. Therefore, it is important to recognize the actual condition of blood diseases in association with the oral treatment of patients. During the period from Sept. 1979 to June 1989, 68 patients who had blood diseases visited the Pedodontic Clinic of Niigata University Dental Hospital. This report is the investigation of the dental situation of these patients. The result were as follows: 1. The ratio of patients with blood diseases in our clinic was 0.7%. The patients with leukemia were most frequent. 2. Most of the first visit-patients with blood diseases were children aged 6, while the general patients group was younger. The ages of first visit-patients corresponded to the most frequent ages of primary diseases. 3. The majority of their chief complaints were "the treatment of decayed teeth for caries". Others were "the extraction of loose teeth for exchange" or "the diseases of oral soft tissues". 4. The patients visited our clinic from all over Niigata prefecture, some of which visited from a great distance and were introduced by Niigata University Medical Hospital. 5. As to the treatment of decayed teeth for caries, the patients with hemophilia had a large number of treated teeth as compared with the other diseases. In case of leukemia, anaphylactoid purpura and idiopathic thrombocytopenic purpura (ITP), the outpatients generally tend to be subject to more severe caries than the inpatients in Niigata University Medical Hospital. 6. The percentage of recall of patients with blood disease was lower than that of general children at the rate of about 20%.(ABSTRACT TRUNCATED AT 250 WORDS)

Blood Coagulation Disorders↗

PubMed1990

New ligands for human C-reactive protein: calcium-dependent binding with epsilon-aminocaproic acid-agarose and calcium-independent binding with omega-aminohexyl-agarose.

Human C-reactive protein (CRP) is an acute phase protein which increases in concentration in response to inflammation. CRP has been known to bind with phosphorylcholine in a calcium-dependent manner. In this study, CRP is found to bind to affinity chromatography of negatively-charged epsilon-aminocaproic acid-agarose when calcium ions are present, and to the affinity chromatography of positively-charged omega-aminohexyl-agarose if a normal ionic strength of NaCl exists but calcium ion is not present.

Aminocaproates↗

PubMed1990

Assessment of Kupffer cell function in rats with chronic liver injury caused by CCl4.

Kupffer cell function was assessed by using scintigraphy to evaluate the turnover of a metabolizable tracer (99mTc-millimicrosphered albumin). The organ uptake rate of the tracer, and new parameters concerned with the degradative functions of Kupffer cells obtained from analysis of the excretion phase of the hepatic time-uptake rate curve, were measured in rats with two different types of chronic liver damage induced by carbon tetrachloride (fatty liver group and liver cirrhosis group). The hepatic uptake rate in chronic liver injury decreased, while in contrast the splenic and pulmonary uptake rates increased. A particularly high uptake by the lungs was observed. The data demonstrated a reduced phagocytic activity of the Kupffer cells in rats with chronic liver injury. The new parameters concerned with Kupffer cell degradative function; i.e. the excretion rate (K) and the degradation rate in the first 60-min (D60), were markedly decreased even in the early stage of chronic liver injury. The data showed that the impairment of Kupffer cell degradative function occurred even earlier in liver damage than impairment of the phagocytic activity, so that the K value and the D60 value were the more sensitive indicators of Kupffer cell function.

Animals↗

PubMed1990

[Intraperitoneal (i.p.) chemotherapy in the management of ovarian cancer--pharmacokinetics and methods of sequential i.p. chemotherapy].

We studied the systemic efficacy of intraperitoneal (i.p.) chemotherapy which has been used as a locoregional chemotherapy for ovarian cancer. The following results were obtained. 1) The CDDP-based i.v. and i.p. regimen obtained a 90% response rate for ascites and a 35% response rate for measurable lesions. 2) According to the pharmacokinetic analysis of i.p.-chemotherapy, BH-AC showed a regional advantage because of a high AUC-ratio. Meanwhile CDDP and etoposide demonstrated a low AUC-ratio, that has direct effects in the abdominal cavity and anti-tumor effects from the vascular side of the tumor. 3) We performed sequential i.p.-chemotherapy using three types of drainage tubes according to the stage and the size of the residual tumor at operation. The abdomino-centesis using a peel-away introducer for the poor risk patients was very easy and this method could carry out sequential CDDP-i.p.. No patients had signs or symptoms of chemical peritonitis. 4) On the base of these date, we have designed a protocol of i.p. chemotherapy for patients with ovarian cancer.

Adult↗

PubMed1989

The pretectum as a site for relaying dorsal column input to thalamic VL neurons.

Intracellular recording techniques were used to study dorsal column input to 122 feline ventral thalamus (VL) relay neurons, before (61 cells) and after (61 cells) lesioning the pretectum. Prior to the lesions, 75% (46/61) of the neurons responded with short and longer latency postsynaptic potentials to dorsal column stimulation. Latencies of the postsynaptic potentials ranged from 4 (short) to 20 ms (long). After the lesions, only long latency responses were encountered, and those responses were seen in only 16% (10/61) of the cells. These data indicate that the pretectum may play an important role in mediating dorsal column information to VL, ultimately influencing cerebellar commands to the motor cortex.

Action Potentials↗

PubMed1989

Diastolic compliance of the left atrium in man: a determinant of preload of the left ventricle.

During the ventricular slow-filling period, both the left atrium and left ventricle fill passively, and their respective internal pressures equalize, becoming evenly elevated. If the diastolic chamber compliance of the left atrium is smaller than that of the left ventricle, we expect the inflowing blood to be distributed more to the left ventricle than to the left atrium during this period. We examined the magnitude of the diastolic compliance of the left atrium and the left ventricle at the end of the slow-filling period. We studied 10 patients, mostly with a mild degree of coronary artery disease, in whom hemodynamic variables were almost within normal limits. To estimate the compliance of the left atrium, we recorded the left atrial pressure directly (by the Brockenbrough technique) and determined the left atrial volume by biplane cineatriography. We determined the diastolic compliance of the left atrium from the pressure-volume relations between the nadir of the x trough and the peak of the v wave by fitting them to an exponential equation. P = b.eaV (P = pressure, V = volume, a, b = constants). The diastolic compliance of the left ventricle was determined from the pressure-volume relations during the ventricular slow-filling period. The compliances of the left atrium and the left ventricle at the pressure at the end of the ventricular slow-filling period were 1.60 +/- 0.41 (mean +/- SD) ml.mmHg-1.m-2 and 4.22 +/- 1.12, respectively. The ratio of compliance of the left ventricle to that of the left atrium was 2.60 +/- 0.71.(ABSTRACT TRUNCATED AT 250 WORDS)

Blood Pressure↗

PubMed1989

Effects of human PTH-related peptide and human PTH on cyclic AMP production and cytosolic free calcium in an osteoblastic cell clone.

Recently, human parathyroid hormone-related peptide (hPTHrP) has been purified and its amino acid sequence determined. Within the amino-terminal 13 residues of hPTHrP, 8 amino acids were found homologous between hPTHrP and human PTH (hPTH). This peptide was reported to stimulate cyclic AMP (cAMP) production in osteoblastic cell lines (UMR106 and ROS17/2.8). However, whether or not this peptide affects another second messenger, i.e., cytosolic free calcium ([Ca2+]i), in osteoblasts has not yet been determined. Therefore, in the present study, we examined the effects of synthetic amino-terminal fragments of hPTHrP (Tyr40hPTHrP1-40 and hPTHrP1-34) on intracellular cAMP production and [Ca2+]i in an osteoblastic cell line (MC3T3-E1) and compared them with those of hPTH1-34. Human PTHrP1-34, Tyr40hPTHrP1-40 and hPTH1-34 stimulated cAMP production in an equipotent manner at concentrations ranging from 2.5 x 10(-10) to 1.3 x 10(-6) M. Human PTH1-34 at concentrations from 2.5 x 10(-7) to 1.3 x 10(-6) M significantly (P less than 0.05) increased [Ca2+]i, but hPTHrP1-34 and Tyr40hPTHrP1-40 at the same concentrations did not. These results suggest a different receptor-mediated mechanism for [Ca2+]i increase between hPTHrP and hPTH, although these two peptides appear to share the same receptor site(s) which is coupled to the cAMP system in MC3T3-E1.

Animals↗

PubMed1989

Serotonin- and catecholamine-related substances in the brain of ornithine transcarbamylase-deficient Sparse-fur mice in the hyperammonemic state: comparison of two procedures for obtaining brain extract, decapitation and microwave irradiation.

Increased flux through the 5-HT pathway during hyperammonemia was indicated by a significant positive correlation between Gln concentration and the ratio of 5-HIAA to 5-HT in the brain of spf mice taken after microwave irradiation to prevent postmortem changes in metabolites. There were no significant changes in the catecholamine pathway in the brain during hyperammonemia despite the increases in Try and Phe.

Ammonia↗